UniProtKB - Q01628 (IFM3_HUMAN)
Protein
Interferon-induced transmembrane protein 3
Gene
IFITM3
Organism
Homo sapiens (Human)
Status
Functioni
IFN-induced antiviral protein which disrupts intracellular cholesterol homeostasis. Inhibits the entry of viruses to the host cell cytoplasm by preventing viral fusion with cholesterol depleted endosomes. May inactivate new enveloped viruses which buds out of the infected cell, by letting them go out with a cholesterol depleted membrane. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV), human immunodeficiency virus type 1 (HIV-1) and vesicular stomatitis virus (VSV). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry, SARS-CoV S protein-mediated viral entry and VSV G protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state.8 Publications
GO - Biological processi
- defense response to virus Source: UniProtKB
- immune response Source: ProtInc
- negative regulation of viral entry into host cell Source: UniProtKB
- negative regulation of viral genome replication Source: UniProtKB
- negative regulation of viral transcription Source: UniProtKB
- response to interferon-alpha Source: UniProtKB
- response to interferon-beta Source: UniProtKB
- response to interferon-gamma Source: UniProtKB
- response to virus Source: UniProtKB
- type I interferon signaling pathway Source: GO_Central
Keywordsi
Biological process | Antiviral defense, Immunity, Innate immunity |
Enzyme and pathway databases
PathwayCommonsi | Q01628 |
Reactomei | R-HSA-909733, Interferon alpha/beta signaling |
Protein family/group databases
TCDBi | 8.A.58.1.3, the dispanin (dispanin) family |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon-induced transmembrane protein 3Alternative name(s): Dispanin subfamily A member 2b Short name: DSPA2b Interferon-inducible protein 1-8U |
Gene namesi | Name:IFITM3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:5414, IFITM3 |
MIMi | 605579, gene |
neXtProti | NX_Q01628 |
VEuPathDBi | HostDB:ENSG00000142089.15 |
Subcellular locationi
Lysosome
- Lysosome membrane 1 Publication; Single-pass type II membrane protein Curated
Plasma membrane
- Cell membrane 2 Publications; Single-pass type II membrane protein 2 Publications
Endosome
- Late endosome membrane 1 Publication; Single-pass type II membrane protein Curated
Other locations
- perinuclear region 1 Publication
Note: Co-localizes with BRI3 isoform 1 at the perinuclear region.1 Publication
Endosome
- late endosome membrane Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- protein-containing complex Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 57 | CytoplasmicSequence analysisAdd BLAST | 57 | |
Intramembranei | 58 – 78 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 79 – 107 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 108 – 128 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 129 – 133 | ExtracellularSequence analysis | 5 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Endosome, Lysosome, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 10410 |
MalaCardsi | IFITM3 |
MIMi | 614680, phenotype |
OpenTargetsi | ENSG00000142089 |
PharmGKBi | PA29655 |
Miscellaneous databases
Pharosi | Q01628, Tbio |
Genetic variation databases
BioMutai | IFITM3 |
DMDMi | 20178301 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153729 | 1 – 133 | Interferon-induced transmembrane protein 3Add BLAST | 133 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 24 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Lipidationi | 71 | S-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 72 | S-palmitoyl cysteine1 Publication | 1 | |
Cross-linki | 83 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 88 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 104 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Lipidationi | 105 | S-palmitoyl cysteine1 Publication | 1 |
Post-translational modificationi
Palmitoylation on membrane-proximal cysteines controls clustering in membrane compartments and antiviral activity against influenza virus.2 Publications
Not glycosylated.
Polyubiquitinated with both 'Lys-48' and 'Lys-63' linkages. Ubiquitination negatively regulates antiviral activity. Lys-24 is the most prevalent ubiquitination site.1 Publication
Keywords - PTMi
Isopeptide bond, Lipoprotein, Palmitate, Ubl conjugationProteomic databases
EPDi | Q01628 |
jPOSTi | Q01628 |
MassIVEi | Q01628 |
PaxDbi | Q01628 |
PeptideAtlasi | Q01628 |
PRIDEi | Q01628 |
ProteomicsDBi | 57973 |
TopDownProteomicsi | Q01628 |
PTM databases
iPTMneti | Q01628 |
PhosphoSitePlusi | Q01628 |
SwissPalmi | Q01628 |
Expressioni
Inductioni
By IFN-alpha and IFNG/IFN-gamma.
Gene expression databases
Bgeei | ENSG00000142089, Expressed in ectocervix and 242 other tissues |
ExpressionAtlasi | Q01628, baseline and differential |
Genevisiblei | Q01628, HS |
Organism-specific databases
HPAi | ENSG00000142089, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with ATP6V0B (By similarity).
Interacts with CD81 (By similarity).
Interacts with SPP1; the interaction reduces OPN expression (PubMed:19901966).
Interacts with VAPA (PubMed:23601107).
Interacts with BRI3 (isoforms 1 and 2); the interaction with isoform 2 is weaker than with isoform 1 (PubMed:30983867).
By similarity3 PublicationsBinary interactionsi
Hide detailsQ01628
Protein-protein interaction databases
BioGRIDi | 115681, 108 interactors |
IntActi | Q01628, 29 interactors |
MINTi | Q01628 |
STRINGi | 9606.ENSP00000382707 |
Miscellaneous databases
RNActi | Q01628, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 60 – 93 | Interaction with SPP11 PublicationAdd BLAST | 34 | |
Regioni | 108 – 133 | Interaction with VAPA1 PublicationAdd BLAST | 26 |
Sequence similaritiesi
Belongs to the CD225/Dispanin family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S9XK, Eukaryota |
GeneTreei | ENSGT00950000182857 |
InParanoidi | Q01628 |
OMAi | TLYLNFC |
OrthoDBi | 1555189at2759 |
PhylomeDBi | Q01628 |
TreeFami | TF334894 |
Family and domain databases
InterProi | View protein in InterPro IPR007593, CD225/Dispanin_fam |
Pfami | View protein in Pfam PF04505, CD225, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q01628-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNHTVQTFFS PVNSGQPPNY EMLKEEHEVA VLGAPHNPAP PTSTVIHIRS
60 70 80 90 100
ETSVPDHVVW SLFNTLFMNP CCLGFIAFAY SVKSRDRKMV GDVTGAQAYA
110 120 130
STAKCLNIWA LILGILMTIL LIVIPVLIFQ AYG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PS44 | E9PS44_HUMAN | Interferon-induced transmembrane pr... | IFITM3 | 112 | Annotation score: | ||
R4GNG9 | R4GNG9_HUMAN | Interferon-induced transmembrane pr... | IFITM3 | 55 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | N → S in CAA40626 (PubMed:1906403).Curated | 1 | |
Sequence conflicti | 34 | A → G in CAA40626 (PubMed:1906403).Curated | 1 |
Polymorphismi
Genetic variations in IFITM3 are responsible for susceptibility to severe influenza virus infection [MIMi:614680].2 Publications
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_053810 | 3 | H → Q1 PublicationCorresponds to variant dbSNP:rs1136853Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57352 mRNA Translation: CAA40626.1 JQ610571 Genomic DNA Translation: AFF60305.1 JQ610572 Genomic DNA Translation: AFF60306.1 JQ610573 Genomic DNA Translation: AFF60307.1 JQ610574 Genomic DNA Translation: AFF60308.1 JQ610575 Genomic DNA Translation: AFF60309.1 JQ610576 Genomic DNA Translation: AFF60310.1 JQ610577 Genomic DNA Translation: AFF60311.1 JQ610578 Genomic DNA Translation: AFF60312.1 JQ610579 Genomic DNA Translation: AFF60313.1 JQ610580 Genomic DNA Translation: AFF60314.1 JQ610581 Genomic DNA Translation: AFF60315.1 JQ610582 Genomic DNA Translation: AFF60316.1 JQ610583 Genomic DNA Translation: AFF60317.1 JQ610585 Genomic DNA Translation: AFF60319.1 JQ610586 Genomic DNA Translation: AFF60320.1 JQ610587 Genomic DNA Translation: AFF60321.1 JQ610588 Genomic DNA Translation: AFF60322.1 JQ610589 Genomic DNA Translation: AFF60323.1 JQ610590 Genomic DNA Translation: AFF60324.1 JQ610591 Genomic DNA Translation: AFF60325.1 JQ610592 Genomic DNA Translation: AFF60326.1 JQ610593 Genomic DNA Translation: AFF60327.1 JQ610594 Genomic DNA Translation: AFF60328.1 JQ610595 Genomic DNA Translation: AFF60329.1 JQ610596 Genomic DNA Translation: AFF60330.1 JQ610597 Genomic DNA Translation: AFF60331.1 JQ610598 Genomic DNA Translation: AFF60332.1 JQ610599 Genomic DNA Translation: AFF60333.1 JQ610600 Genomic DNA Translation: AFF60334.1 JQ610601 Genomic DNA Translation: AFF60335.1 JQ610602 Genomic DNA Translation: AFF60336.1 JQ610603 Genomic DNA Translation: AFF60337.1 JQ610604 Genomic DNA Translation: AFF60338.1 JQ610605 Genomic DNA Translation: AFF60339.1 JQ610606 Genomic DNA Translation: AFF60340.1 JQ610607 Genomic DNA Translation: AFF60341.1 JQ610608 Genomic DNA Translation: AFF60342.1 JQ610609 Genomic DNA Translation: AFF60343.1 JQ610610 Genomic DNA Translation: AFF60344.1 JQ610611 Genomic DNA Translation: AFF60345.1 JQ610613 Genomic DNA Translation: AFF60347.1 JQ610614 Genomic DNA Translation: AFF60348.1 JQ610615 Genomic DNA Translation: AFF60349.1 JQ610616 Genomic DNA Translation: AFF60350.1 JQ610617 Genomic DNA Translation: AFF60351.1 JQ610618 Genomic DNA Translation: AFF60352.1 JQ610620 Genomic DNA Translation: AFF60354.1 JQ610621 Genomic DNA Translation: AFF60355.1 BT006892 mRNA Translation: AAP35538.1 AK292173 mRNA Translation: BAF84862.1 AC136475 Genomic DNA No translation available. CH891444 Genomic DNA Translation: EAW50866.1 BC006794 mRNA Translation: AAH06794.1 BC008417 mRNA Translation: AAH08417.1 BC022439 mRNA Translation: AAH22439.1 BC070243 mRNA Translation: AAH70243.1 |
CCDSi | CCDS41585.1 |
PIRi | S17182 |
RefSeqi | NP_066362.2, NM_021034.2 |
Genome annotation databases
Ensembli | ENST00000399808; ENSP00000382707; ENSG00000142089 |
GeneIDi | 10410 |
KEGGi | hsa:10410 |
UCSCi | uc001lpa.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57352 mRNA Translation: CAA40626.1 JQ610571 Genomic DNA Translation: AFF60305.1 JQ610572 Genomic DNA Translation: AFF60306.1 JQ610573 Genomic DNA Translation: AFF60307.1 JQ610574 Genomic DNA Translation: AFF60308.1 JQ610575 Genomic DNA Translation: AFF60309.1 JQ610576 Genomic DNA Translation: AFF60310.1 JQ610577 Genomic DNA Translation: AFF60311.1 JQ610578 Genomic DNA Translation: AFF60312.1 JQ610579 Genomic DNA Translation: AFF60313.1 JQ610580 Genomic DNA Translation: AFF60314.1 JQ610581 Genomic DNA Translation: AFF60315.1 JQ610582 Genomic DNA Translation: AFF60316.1 JQ610583 Genomic DNA Translation: AFF60317.1 JQ610585 Genomic DNA Translation: AFF60319.1 JQ610586 Genomic DNA Translation: AFF60320.1 JQ610587 Genomic DNA Translation: AFF60321.1 JQ610588 Genomic DNA Translation: AFF60322.1 JQ610589 Genomic DNA Translation: AFF60323.1 JQ610590 Genomic DNA Translation: AFF60324.1 JQ610591 Genomic DNA Translation: AFF60325.1 JQ610592 Genomic DNA Translation: AFF60326.1 JQ610593 Genomic DNA Translation: AFF60327.1 JQ610594 Genomic DNA Translation: AFF60328.1 JQ610595 Genomic DNA Translation: AFF60329.1 JQ610596 Genomic DNA Translation: AFF60330.1 JQ610597 Genomic DNA Translation: AFF60331.1 JQ610598 Genomic DNA Translation: AFF60332.1 JQ610599 Genomic DNA Translation: AFF60333.1 JQ610600 Genomic DNA Translation: AFF60334.1 JQ610601 Genomic DNA Translation: AFF60335.1 JQ610602 Genomic DNA Translation: AFF60336.1 JQ610603 Genomic DNA Translation: AFF60337.1 JQ610604 Genomic DNA Translation: AFF60338.1 JQ610605 Genomic DNA Translation: AFF60339.1 JQ610606 Genomic DNA Translation: AFF60340.1 JQ610607 Genomic DNA Translation: AFF60341.1 JQ610608 Genomic DNA Translation: AFF60342.1 JQ610609 Genomic DNA Translation: AFF60343.1 JQ610610 Genomic DNA Translation: AFF60344.1 JQ610611 Genomic DNA Translation: AFF60345.1 JQ610613 Genomic DNA Translation: AFF60347.1 JQ610614 Genomic DNA Translation: AFF60348.1 JQ610615 Genomic DNA Translation: AFF60349.1 JQ610616 Genomic DNA Translation: AFF60350.1 JQ610617 Genomic DNA Translation: AFF60351.1 JQ610618 Genomic DNA Translation: AFF60352.1 JQ610620 Genomic DNA Translation: AFF60354.1 JQ610621 Genomic DNA Translation: AFF60355.1 BT006892 mRNA Translation: AAP35538.1 AK292173 mRNA Translation: BAF84862.1 AC136475 Genomic DNA No translation available. CH891444 Genomic DNA Translation: EAW50866.1 BC006794 mRNA Translation: AAH06794.1 BC008417 mRNA Translation: AAH08417.1 BC022439 mRNA Translation: AAH22439.1 BC070243 mRNA Translation: AAH70243.1 |
CCDSi | CCDS41585.1 |
PIRi | S17182 |
RefSeqi | NP_066362.2, NM_021034.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 115681, 108 interactors |
IntActi | Q01628, 29 interactors |
MINTi | Q01628 |
STRINGi | 9606.ENSP00000382707 |
Protein family/group databases
TCDBi | 8.A.58.1.3, the dispanin (dispanin) family |
PTM databases
iPTMneti | Q01628 |
PhosphoSitePlusi | Q01628 |
SwissPalmi | Q01628 |
Genetic variation databases
BioMutai | IFITM3 |
DMDMi | 20178301 |
Proteomic databases
EPDi | Q01628 |
jPOSTi | Q01628 |
MassIVEi | Q01628 |
PaxDbi | Q01628 |
PeptideAtlasi | Q01628 |
PRIDEi | Q01628 |
ProteomicsDBi | 57973 |
TopDownProteomicsi | Q01628 |
Protocols and materials databases
Antibodypediai | 1023, 340 antibodies |
DNASUi | 10410 |
Genome annotation databases
Ensembli | ENST00000399808; ENSP00000382707; ENSG00000142089 |
GeneIDi | 10410 |
KEGGi | hsa:10410 |
UCSCi | uc001lpa.3, human |
Organism-specific databases
CTDi | 10410 |
DisGeNETi | 10410 |
GeneCardsi | IFITM3 |
HGNCi | HGNC:5414, IFITM3 |
HPAi | ENSG00000142089, Low tissue specificity |
MalaCardsi | IFITM3 |
MIMi | 605579, gene 614680, phenotype |
neXtProti | NX_Q01628 |
OpenTargetsi | ENSG00000142089 |
PharmGKBi | PA29655 |
VEuPathDBi | HostDB:ENSG00000142089.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S9XK, Eukaryota |
GeneTreei | ENSGT00950000182857 |
InParanoidi | Q01628 |
OMAi | TLYLNFC |
OrthoDBi | 1555189at2759 |
PhylomeDBi | Q01628 |
TreeFami | TF334894 |
Enzyme and pathway databases
PathwayCommonsi | Q01628 |
Reactomei | R-HSA-909733, Interferon alpha/beta signaling |
Miscellaneous databases
BioGRID-ORCSi | 10410, 645 hits in 962 CRISPR screens |
ChiTaRSi | IFITM3, human |
GeneWikii | IFITM3 |
GenomeRNAii | 10410 |
Pharosi | Q01628, Tbio |
PROi | PR:Q01628 |
RNActi | Q01628, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000142089, Expressed in ectocervix and 242 other tissues |
ExpressionAtlasi | Q01628, baseline and differential |
Genevisiblei | Q01628, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007593, CD225/Dispanin_fam |
Pfami | View protein in Pfam PF04505, CD225, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IFM3_HUMAN | |
Accessioni | Q01628Primary (citable) accession number: Q01628 Secondary accession number(s): Q53Y76, Q96HK8, Q96J15 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | April 16, 2002 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families