UniProtKB - Q01519 (COX12_YEAST)
Cytochrome c oxidase subunit 12, mitochondrial
COX12
Functioni
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of COX2 and heme A of COX1 to the active site in COX1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
1 PublicationMiscellaneous
: oxidative phosphorylation Pathwayi
This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.
GO - Molecular functioni
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- cellular respiration Source: ComplexPortal
- mitochondrial cytochrome c oxidase assembly Source: SGD
- mitochondrial electron transport, cytochrome c to oxygen Source: ComplexPortal
- proton transmembrane transport Source: GOC
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
UniPathwayi | UPA00705 |
Protein family/group databases
TCDBi | 3.D.4.8.1, the proton-translocating cytochrome oxidase (cox) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c oxidase subunit 12, mitochondrialAlternative name(s): Cytochrome c oxidase polypeptide VIb |
Gene namesi | Name:COX12 Ordered Locus Names:YLR038C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004028, COX12 |
VEuPathDBi | FungiDB:YLR038C |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 2 Publications
Note: Imported into the mitochondria via the mitochondrial MIA40-ERV1 machinery.
Mitochondrion
- mitochondrial intermembrane space Source: SGD
- mitochondrial membrane Source: ComplexPortal
- mitochondrial respiratory chain complex IV Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed3 Publications | |||
ChainiPRO_0000194925 | 2 – 83 | Cytochrome c oxidase subunit 12, mitochondrialAdd BLAST | 82 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 27 ↔ 59 | PROSITE-ProRule annotation | ||
Disulfide bondi | 37 ↔ 48 | PROSITE-ProRule annotation | ||
Modified residuei | 82 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
MaxQBi | Q01519 |
PaxDbi | Q01519 |
PRIDEi | Q01519 |
2D gel databases
UCD-2DPAGEi | Q01519 |
PTM databases
iPTMneti | Q01519 |
Interactioni
Subunit structurei
Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 12 subunits. The complex is composed of a catalytic core of 3 subunits COX1, COX2 and COX3, encoded in the mitochondrial DNA, and 9 supernumerary subunits COX4, COX5A (or COX5B), COX6, COX7, COX8, COX9, COX12, COX13 and COX26, which are encoded in the nuclear genome (PubMed:7851399, PubMed:30598556, PubMed:30598554). The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with a dimer of ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), resulting in 2 different assemblies (supercomplexes III2IV and III2IV2) (PubMed:10775262, PubMed:10764779, PubMed:30598556, PubMed:30598554).
5 PublicationsProtein-protein interaction databases
BioGRIDi | 31314, 299 interactors |
ComplexPortali | CPX-1721, Mitochondrial respiratory chain complex IV, COX5A variant CPX-1722, Mitochondrial respiratory chain complex IV, COX5B variant |
DIPi | DIP-4520N |
IntActi | Q01519, 24 interactors |
STRINGi | 4932.YLR038C |
Miscellaneous databases
RNActi | Q01519, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q01519 |
SMRi | Q01519 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 67 | CHCHPROSITE-ProRule annotationAdd BLAST | 44 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 27 – 37 | Cx9C motifPROSITE-ProRule annotationAdd BLAST | 11 | |
Motifi | 48 – 59 | Cx10C motifPROSITE-ProRule annotationAdd BLAST | 12 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3057, Eukaryota |
GeneTreei | ENSGT00940000174930 |
HOGENOMi | CLU_133964_2_0_1 |
InParanoidi | Q01519 |
OMAi | CPNEWIA |
Family and domain databases
CDDi | cd00926, Cyt_c_Oxidase_VIb, 1 hit |
Gene3Di | 1.10.10.140, 1 hit |
InterProi | View protein in InterPro IPR003213, Cyt_c_oxidase_su6B IPR036549, Cyt_c_oxidase_su6B_sf |
PANTHERi | PTHR11387, PTHR11387, 1 hit |
Pfami | View protein in Pfam PF02297, COX6B, 1 hit |
PIRSFi | PIRSF000278, Cyt_c_oxidase_6B, 1 hit |
SUPFAMi | SSF47694, SSF47694, 1 hit |
PROSITEi | View protein in PROSITE PS51808, CHCH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MADQENSPLH TVGFDARFPQ QNQTKHCWQS YVDYHKCVNM KGEDFAPCKV
60 70 80
FWKTYNALCP LDWIEKWDDQ REKGIFAGDI NSD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98332 Genomic DNA Translation: AAA34510.1 Z73210 Genomic DNA Translation: CAA97566.1 AY557925 Genomic DNA Translation: AAS56251.1 BK006945 Genomic DNA Translation: DAA09357.1 |
PIRi | S31256 |
RefSeqi | NP_013139.1, NM_001181925.1 |
Genome annotation databases
EnsemblFungii | YLR038C_mRNA; YLR038C; YLR038C |
GeneIDi | 850727 |
KEGGi | sce:YLR038C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M98332 Genomic DNA Translation: AAA34510.1 Z73210 Genomic DNA Translation: CAA97566.1 AY557925 Genomic DNA Translation: AAS56251.1 BK006945 Genomic DNA Translation: DAA09357.1 |
PIRi | S31256 |
RefSeqi | NP_013139.1, NM_001181925.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6GIQ | electron microscopy | 3.23 | j | 1-83 | [»] | |
6HU9 | electron microscopy | 3.35 | j/v | 2-83 | [»] | |
6T0B | electron microscopy | 2.80 | j/w | 2-83 | [»] | |
6T15 | electron microscopy | 3.29 | j | 2-83 | [»] | |
6YMX | electron microscopy | 3.17 | j | 6-83 | [»] | |
6YMY | electron microscopy | 3.41 | j | 6-83 | [»] | |
AlphaFoldDBi | Q01519 | |||||
SMRi | Q01519 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31314, 299 interactors |
ComplexPortali | CPX-1721, Mitochondrial respiratory chain complex IV, COX5A variant CPX-1722, Mitochondrial respiratory chain complex IV, COX5B variant |
DIPi | DIP-4520N |
IntActi | Q01519, 24 interactors |
STRINGi | 4932.YLR038C |
Protein family/group databases
TCDBi | 3.D.4.8.1, the proton-translocating cytochrome oxidase (cox) superfamily |
PTM databases
iPTMneti | Q01519 |
2D gel databases
UCD-2DPAGEi | Q01519 |
Proteomic databases
MaxQBi | Q01519 |
PaxDbi | Q01519 |
PRIDEi | Q01519 |
Genome annotation databases
EnsemblFungii | YLR038C_mRNA; YLR038C; YLR038C |
GeneIDi | 850727 |
KEGGi | sce:YLR038C |
Organism-specific databases
SGDi | S000004028, COX12 |
VEuPathDBi | FungiDB:YLR038C |
Phylogenomic databases
eggNOGi | KOG3057, Eukaryota |
GeneTreei | ENSGT00940000174930 |
HOGENOMi | CLU_133964_2_0_1 |
InParanoidi | Q01519 |
OMAi | CPNEWIA |
Enzyme and pathway databases
UniPathwayi | UPA00705 |
Miscellaneous databases
PROi | PR:Q01519 |
RNActi | Q01519, protein |
Family and domain databases
CDDi | cd00926, Cyt_c_Oxidase_VIb, 1 hit |
Gene3Di | 1.10.10.140, 1 hit |
InterProi | View protein in InterPro IPR003213, Cyt_c_oxidase_su6B IPR036549, Cyt_c_oxidase_su6B_sf |
PANTHERi | PTHR11387, PTHR11387, 1 hit |
Pfami | View protein in Pfam PF02297, COX6B, 1 hit |
PIRSFi | PIRSF000278, Cyt_c_oxidase_6B, 1 hit |
SUPFAMi | SSF47694, SSF47694, 1 hit |
PROSITEi | View protein in PROSITE PS51808, CHCH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | COX12_YEAST | |
Accessioni | Q01519Primary (citable) accession number: Q01519 Secondary accession number(s): D6VY41, Q9URA6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XII
Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families