UniProtKB - Q01454 (CTF4_YEAST)
Protein
DNA polymerase alpha-binding protein
Gene
CTF4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Accessory factor for DNA replication. It plays a role in accurately duplicating the genome in vivo.
Miscellaneous
Present with 3280 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- chromatin binding Source: SGD
- identical protein binding Source: IntAct
GO - Biological processi
- cellular response to DNA damage stimulus Source: GO_Central
- DNA-dependent DNA replication Source: SGD
- DNA repair Source: SGD
- DNA replication initiation Source: SGD
- double-strand break repair via break-induced replication Source: SGD
- establishment of sister chromatid cohesion Source: SGD
- mitotic cell cycle Source: GO_Central
- mitotic sister chromatid cohesion Source: SGD
Keywordsi
Biological process | DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: DNA polymerase alpha-binding proteinAlternative name(s): Chromosome replication protein CHL15 Chromosome transmission fidelity protein 4 Protein POB1 |
Gene namesi | Name:CTF4 Synonyms:CHL15, POB1 Ordered Locus Names:YPR135W ORF Names:P9659.7 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006339, CTF4 |
VEuPathDBi | FungiDB:YPR135W |
Subcellular locationi
Nucleus
Nucleus
- nuclear chromosome Source: SGD
- nuclear replication fork Source: SGD
- nucleus Source: SGD
- replication fork protection complex Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000050948 | 2 – 927 | DNA polymerase alpha-binding proteinAdd BLAST | 926 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 377 | PhosphoserineCombined sources | 1 | |
Modified residuei | 379 | PhosphoserineCombined sources | 1 | |
Modified residuei | 398 | PhosphoserineCombined sources | 1 | |
Modified residuei | 401 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 411 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 463 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q01454 |
PaxDbi | Q01454 |
PRIDEi | Q01454 |
PTM databases
iPTMneti | Q01454 |
Interactioni
Binary interactionsi
Hide detailsQ01454
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 36302, 608 interactors |
ComplexPortali | CPX-1165, CUL8-MMS1-MMS22-CTF4 E3 ubiquitin ligase complex |
DIPi | DIP-4640N |
IntActi | Q01454, 27 interactors |
MINTi | Q01454 |
STRINGi | 4932.YPR135W |
Miscellaneous databases
RNActi | Q01454, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q01454 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 10 – 49 | WD 1Add BLAST | 40 | |
Repeati | 134 – 173 | WD 2Add BLAST | 40 | |
Repeati | 227 – 266 | WD 3Add BLAST | 40 | |
Repeati | 273 – 313 | WD 4Add BLAST | 41 | |
Repeati | 699 – 739 | WD 5Add BLAST | 41 |
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG1274, Eukaryota |
GeneTreei | ENSGT00390000002030 |
HOGENOMi | CLU_004219_2_0_1 |
InParanoidi | Q01454 |
OMAi | NAWFPIC |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR022100, Mcl1_mid IPR015943, WD40/YVTN_repeat-like_dom_sf IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12341, Mcl1_mid, 1 hit |
SUPFAMi | SSF50978, SSF50978, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q01454-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET
60 70 80 90 100
LDSSKLVSSI KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC
110 120 130 140 150
CVIHSGKMAV FGGDDLELIL LELDDETHKK HAIKIDEQVS QISYNSQMNI
160 170 180 190 200
LAVSMINGKV QIFSLTSTIP NKVHELNDYI VANSYDDTHR DKILSNMMDD
210 220 230 240 250
IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK GLHFALPCAD
260 270 280 290 300
DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL
310 320 330 340 350
TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN
360 370 380 390 400
LAESVVSNIP DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI
410 420 430 440 450
TQEANAEDVF TQTHDGPSGL SEKRKYNFED EEDFIDDDDG AGYISGKKPH
460 470 480 490 500
NEHSYSRVHK THSFPISLAN TGKFRYMPFS PAGTPFGFTD RRYLTMNEVG
510 520 530 540 550
YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN EKGTLFGQSK
560 570 580 590 600
TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF
610 620 630 640 650
NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY
660 670 680 690 700
YKRECPLPMS LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS
710 720 730 740 750
DNTLLLLSKW RSPEESKWLP ILDSNMEIWK MSGGKETTDI HVWPLALAYD
760 770 780 790 800
TLNCILVKGK HIWPEFPLPL PSEMEIRMPV FVKSKLLEEN KAILNKKNEI
810 820 830 840 850
GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT LENDGEMYGN
860 870 880 890 900
ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK
910 920
ISERAELPSL VKKINNIREA RYEQQLK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | G → E (PubMed:1341195).Curated | 1 | |
Sequence conflicti | 112 | G → E (PubMed:8316240).Curated | 1 | |
Sequence conflicti | 588 | V → F (PubMed:1341195).Curated | 1 | |
Sequence conflicti | 590 | V → F (PubMed:1341195).Curated | 1 | |
Sequence conflicti | 601 | N → I (PubMed:1341195).Curated | 1 | |
Sequence conflicti | 835 | E → K (PubMed:1341195).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94769 Genomic DNA Translation: AAA34887.1 S63246 Genomic DNA Translation: AAB27308.1 U40829 Genomic DNA Translation: AAB68276.1 BK006949 Genomic DNA Translation: DAA11548.1 |
PIRi | A45039 |
RefSeqi | NP_015461.1, NM_001184232.1 |
Genome annotation databases
EnsemblFungii | YPR135W_mRNA; YPR135W; YPR135W |
GeneIDi | 856254 |
KEGGi | sce:YPR135W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M94769 Genomic DNA Translation: AAA34887.1 S63246 Genomic DNA Translation: AAB27308.1 U40829 Genomic DNA Translation: AAB68276.1 BK006949 Genomic DNA Translation: DAA11548.1 |
PIRi | A45039 |
RefSeqi | NP_015461.1, NM_001184232.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4C8H | X-ray | 2.69 | A/B/C | 471-927 | [»] | |
4C8S | X-ray | 3.00 | A/B/C | 471-927 | [»] | |
4C93 | X-ray | 2.69 | A/B/C | 471-927 | [»] | |
4C95 | X-ray | 2.69 | A/B/C | 471-927 | [»] | |
5HOG | X-ray | 3.09 | A/B/C | 471-927 | [»] | |
5HOI | X-ray | 3.30 | A/B/C | 472-927 | [»] | |
5NXQ | X-ray | 2.41 | A/B/C | 471-927 | [»] | |
6PTJ | electron microscopy | 3.80 | E/F/G | 1-927 | [»] | |
6PTN | electron microscopy | 5.80 | E/F/G | 1-927 | [»] | |
6PTO | electron microscopy | 7.00 | X/Y/Z | 1-927 | [»] | |
6SKL | electron microscopy | 3.70 | F/G/H | 1-927 | [»] | |
SMRi | Q01454 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36302, 608 interactors |
ComplexPortali | CPX-1165, CUL8-MMS1-MMS22-CTF4 E3 ubiquitin ligase complex |
DIPi | DIP-4640N |
IntActi | Q01454, 27 interactors |
MINTi | Q01454 |
STRINGi | 4932.YPR135W |
PTM databases
iPTMneti | Q01454 |
Proteomic databases
MaxQBi | Q01454 |
PaxDbi | Q01454 |
PRIDEi | Q01454 |
Genome annotation databases
EnsemblFungii | YPR135W_mRNA; YPR135W; YPR135W |
GeneIDi | 856254 |
KEGGi | sce:YPR135W |
Organism-specific databases
SGDi | S000006339, CTF4 |
VEuPathDBi | FungiDB:YPR135W |
Phylogenomic databases
eggNOGi | KOG1274, Eukaryota |
GeneTreei | ENSGT00390000002030 |
HOGENOMi | CLU_004219_2_0_1 |
InParanoidi | Q01454 |
OMAi | NAWFPIC |
Miscellaneous databases
PROi | PR:Q01454 |
RNActi | Q01454, protein |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR022100, Mcl1_mid IPR015943, WD40/YVTN_repeat-like_dom_sf IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12341, Mcl1_mid, 1 hit |
SUPFAMi | SSF50978, SSF50978, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CTF4_YEAST | |
Accessioni | Q01454Primary (citable) accession number: Q01454 Secondary accession number(s): D6W4D2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references