DNA polymerase alpha-binding protein

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• chromatin binding Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Ctf4p facilitates Mcm10p to promote DNA replication in budding yeast."
    Wang J., Wu R., Lu Y., Liang C.
    Biochem Biophys Res Commun 395:336-341(2010) [PubMed] [Europe PMC] [Abstract]
• identical protein binding Source: IntAct <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "Defining the budding yeast chromatin-associated interactome."
    Lambert J.P., Fillingham J., Siahbazi M., Greenblatt J., Baetz K., Figeys D.
    Mol Syst Biol 6:448-448(2010) [PubMed] [Europe PMC] [Abstract]
  • "A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome."
    Simon A.C., Zhou J.C., Perera R.L., van Deursen F., Evrin C., Ivanova M.E., Kilkenny M.L., Renault L., Kjaer S., Matak-Vinkovic D., Labib K., Costa A., Pellegrini L.
    Nature 510:293-297(2014) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• cellular response to DNA damage stimulus Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• DNA-dependent DNA replication Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • Ref.1

    "Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo."
    Miles J., Formosa T.
    Mol. Cell. Biol. 12:5724-5735(1992) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
• DNA repair Source: SGDInferred from mutant phenotypeⁱ
  • Ref.2

    "CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae."
    Kouprina N.Y., Kroll E.S., Bannikov V.M., Bliskovsky V.V., Gizatullin R.Z., Kirillov A.V., Shestopalov B.V., Zakharyev V.M., Hieter P., Spencer F., Larionov V.
    Mol. Cell. Biol. 12:5736-5747(1992) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  • Ref.1

    "Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo."
    Miles J., Formosa T.
    Mol. Cell. Biol. 12:5724-5735(1992) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21.
• DNA replication initiation Source: SGDInferred from mutant phenotypeⁱ
  • "Identification of novel factors involved in or regulating initiation of DNA replication by a genome-wide phenotypic screen in Saccharomyces cerevisiae."
    Ma L., Zhai Y., Feng D., Chan T.C., Lu Y., Fu X., Wang J., Chen Y., Li J., Xu K., Liang C.
    Cell Cycle 9:4399-4410(2010) [PubMed] [Europe PMC] [Abstract]
• double-strand break repair via break-induced replication Source: SGDInferred from mutant phenotypeⁱ
  • "Break-induced replication requires all essential DNA replication factors except those specific for pre-RC assembly."
    Lydeard J.R., Lipkin-Moore Z., Sheu Y.J., Stillman B., Burgers P.M., Haber J.E.
    Genes Dev 24:1133-1144(2010) [PubMed] [Europe PMC] [Abstract]
• establishment of sister chromatid cohesion Source: SGDInferred from mutant phenotypeⁱ
  • "An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae."
    Borges V., Smith D.J., Whitehouse I., Uhlmann F.
    Chromosoma 122:121-134(2013) [PubMed] [Europe PMC] [Abstract]
• mitotic cell cycle Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• mitotic sister chromatid cohesion Source: SGDInferred from mutant phenotypeⁱ
  • "Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion."
    Hanna J.S., Kroll E.S., Lundblad V., Spencer F.A.
    Mol. Cell. Biol. 21:3144-3158(2001) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• identical protein binding Source: IntActInferred from physical interactionⁱ
  • "Defining the budding yeast chromatin-associated interactome."
    Lambert J.P., Fillingham J., Siahbazi M., Greenblatt J., Baetz K., Figeys D.
    Mol Syst Biol 6:448-448(2010) [PubMed] [Europe PMC] [Abstract]
  • "A Ctf4 trimer couples the CMG helicase to DNA polymerase alpha in the eukaryotic replisome."
    Simon A.C., Zhou J.C., Perera R.L., van Deursen F., Evrin C., Ivanova M.E., Kilkenny M.L., Renault L., Kjaer S., Matak-Vinkovic D., Labib K., Costa A., Pellegrini L.
    Nature 510:293-297(2014) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingⁱ: The displayed sequence is further processed into a mature form.

        10         20         30         40         50
MVSVIDKLVF DFGGKTLVSL APDNNTLCVA NKNGLTKILK TNNPEEEPET 
        60         70         80         90        100
LDSSKLVSSI KCYSNSHFLM TTMQGDALRY NIDSSQEELL ARFALPLRDC 
       110        120        130        140        150
CVIHSGKMAV FGGDDLELIL LELDDETHKK HAIKIDEQVS QISYNSQMNI 
       160        170        180        190        200
LAVSMINGKV QIFSLTSTIP NKVHELNDYI VANSYDDTHR DKILSNMMDD 
       210        220        230        240        250
IDKDNDNDLS ETADPDENNV ADPEFCAANR ICTRVAWHPK GLHFALPCAD 
       260        270        280        290        300
DTVKIFSIKG YSLQKTLSTN LSSTKAHFID LQFDPLRGTY IAAVDLNNKL 
       310        320        330        340        350
TVWNWETSEI HYTREFKRKI TNIAWKIQAD SKTLDLVLGT WSGSIAIVQN 
       360        370        380        390        400
LAESVVSNIP DQSVAESSTK HGLFVDSESD LENLEGNDDI NKSDKLFSDI 
       410        420        430        440        450
TQEANAEDVF TQTHDGPSGL SEKRKYNFED EEDFIDDDDG AGYISGKKPH 
       460        470        480        490        500
NEHSYSRVHK THSFPISLAN TGKFRYMPFS PAGTPFGFTD RRYLTMNEVG 
       510        520        530        540        550
YVSTVKNSEQ YSITVSFFDV GRFREYHFED LFGYDLCFLN EKGTLFGQSK 
       560        570        580        590        600
TGQIQYRPHD SIHSNWTKII PLQAGERITS VAATPVRVIV GTSLGYFRSF 
       610        620        630        640        650
NQFGVPFAVE KTSPIVALTA QNYRVFSVHY SQFHGLSYSL SELGTSSKRY 
       660        670        680        690        700
YKRECPLPMS LPNINSDMKK DANLDYYNFN PMGIKSLFFS SYGDPCIFGS 
       710        720        730        740        750
DNTLLLLSKW RSPEESKWLP ILDSNMEIWK MSGGKETTDI HVWPLALAYD 
       760        770        780        790        800
TLNCILVKGK HIWPEFPLPL PSEMEIRMPV FVKSKLLEEN KAILNKKNEI 
       810        820        830        840        850
GADTEAEEGE EDKEIQIPVS MAAEEEYLRS KVLSELLTDT LENDGEMYGN 
       860        870        880        890        900
ENEVLAALNG AYDKALLRLF ASACSDQNVE KALSLAHELK QDRALTAAVK 
       910        920 
ISERAELPSL VKKINNIREA RYEQQLK                          

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
2. Yeast chromosome XVI
   Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
3. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references