UniProtKB - Q01217 (ARG56_YEAST)
Protein
Protein ARG5,6, mitochondrial
Gene
ARG5,6
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 1180 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = H+ + N-acetyl-L-glutamyl 5-phosphate + NADPHEC:1.2.1.38
- EC:2.7.2.8
Activity regulationi
The kinase activity is inhibited by arginine.
: L-arginine biosynthesis Pathwayi
This protein is involved in step 2 and 3 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (ARG7), Amino-acid acetyltransferase, mitochondrial (ARG2)
- Protein ARG5,6, mitochondrial (ARG5,6)
- Protein ARG5,6, mitochondrial (ARG5,6)
- Acetylornithine aminotransferase, mitochondrial (ARG8)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 675 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- acetyl-CoA:L-glutamate N-acetyltransferase activity Source: GO_Central
- acetylglutamate kinase activity Source: SGD
- arginine binding Source: GO_Central
- ATP binding Source: UniProtKB-KW
- N-acetyl-gamma-glutamyl-phosphate reductase activity Source: SGD
- NAD binding Source: InterPro
- protein dimerization activity Source: InterPro
GO - Biological processi
- arginine biosynthetic process Source: GO_Central
- ornithine biosynthetic process Source: SGD
- regulation of transcription, DNA-templated Source: SGD
Keywordsi
Molecular function | Kinase, Multifunctional enzyme, Oxidoreductase, Transferase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Ligand | ATP-binding, NADP, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.2.8, 984 |
Reactomei | R-SCE-70635, Urea cycle |
UniPathwayi | UPA00068;UER00107 UPA00068;UER00108 |
Protein family/group databases
MoonProti | Q01217 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein ARG5,6, mitochondrialCleaved into the following 2 chains: Alternative name(s): N-acetyl-glutamate semialdehyde dehydrogenase Short name: NAGSA dehydrogenase Alternative name(s): N-acetyl-L-glutamate 5-phosphotransferase NAG kinase Short name: AGK |
Gene namesi | Name:ARG5,6 Ordered Locus Names:YER069W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YER069W |
SGDi | S000000871, ARG5,6 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrial matrix Source: SGD
- mitochondrion Source: SGD
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 65 | MitochondrionSequence analysisAdd BLAST | 65 | |
ChainiPRO_0000002073 | 66 – 532 | Acetylglutamate kinaseSequence analysisAdd BLAST | 467 | |
ChainiPRO_0000002074 | 533 – 863 | N-acetyl-gamma-glutamyl-phosphate reductaseSequence analysisAdd BLAST | 331 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 359 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
The protein precursor is cleaved into the two biologically active enzymes, the kinase and the reductase.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q01217 |
PaxDbi | Q01217 |
PRIDEi | Q01217 |
PTM databases
iPTMneti | Q01217 |
Interactioni
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 36812, 23 interactors |
ComplexPortali | CPX-1151, N-acetylglutamate synthase NAGS/NAGK complex |
DIPi | DIP-5348N |
IntActi | Q01217, 3 interactors |
MINTi | Q01217 |
STRINGi | 4932.YER069W |
Miscellaneous databases
RNActi | Q01217, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q01217 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 353 – 505 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 153 |
Sequence similaritiesi
In the N-terminal section; belongs to the acetylglutamate kinase family.Curated
In the C-terminal section; belongs to the NAGSA dehydrogenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2436, Eukaryota KOG4354, Eukaryota |
HOGENOMi | CLU_006384_4_0_1 |
InParanoidi | Q01217 |
OMAi | TSRDIRN |
Family and domain databases
CDDi | cd04252, AAK_NAGK-fArgBP, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit |
HAMAPi | MF_00150, ArgC_type1, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR004662, AcgluKinase_fam IPR023013, AGPR_AS IPR000706, AGPR_type-1 IPR001048, Asp/Glu/Uridylate_kinase IPR036291, NAD(P)-bd_dom_sf IPR041734, NAGK-fArgBP IPR011241, NAGK/NAGSA IPR000534, Semialdehyde_DH_NAD-bd IPR012280, Semialdhyde_DH_dimer_dom IPR006855, Vertebrate-like_GNAT_dom |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF04768, NAT, 1 hit PF01118, Semialdhyde_dh, 1 hit PF02774, Semialdhyde_dhC, 1 hit |
PIRSFi | PIRSF036440, ARG5-6, 1 hit |
SMARTi | View protein in SMART SM00859, Semialdhyde_dh, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF53633, SSF53633, 1 hit |
TIGRFAMsi | TIGR00761, argB, 1 hit TIGR01850, argC, 1 hit |
PROSITEi | View protein in PROSITE PS01224, ARGC, 1 hit PS51731, GNAT_NAGS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q01217-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSASLLVST KRLNASKFQK FVSSLNKSTI AGFASVPLRA PPSVAFTRKK
60 70 80 90 100
VGYSKRYVSS TNGFSATRST VIQLLNNIST KREVEQYLKY FTSVSQQQFA
110 120 130 140 150
VIKVGGAIIS DNLHELASCL AFLYHVGLYP IVLHGTGPQV NGRLEAQGIE
160 170 180 190 200
PDYIDGIRIT DEHTMAVVRK CFLEQNLKLV TALEQLGVRA RPITSGVFTA
210 220 230 240 250
DYLDKDKYKL VGNIKSVTKE PIEASIKAGA LPILTSLAET ASGQMLNVNA
260 270 280 290 300
DVAAGELARV FEPLKIVYLN EKGGIINGST GEKISMINLD EEYDDLMKQS
310 320 330 340 350
WVKYGTKLKI REIKELLDYL PRSSSVAIIN VQDLQKELFT DSGAGTMIRR
360 370 380 390 400
GYKLVKRSSI GEFPSADALR KALQRDAGIS SGKESVASYL RYLENSDFVS
410 420 430 440 450
YADEPLEAVA IVKKDTNVPT LDKFVCSDAA WLNNVTDNVF NVLRRDFPAL
460 470 480 490 500
QWVVSENDAN IAWHFDKSQG SYLKGGKVLF WYGIDDINTI SELVENFVKS
510 520 530 540 550
CDTASTLNSS ASSGVFANKK SARSYSTRST PRPEGVNTNP GRVALIGARG
560 570 580 590 600
YTGKNLVSLI NGHPYLEVAH VSSRELKGQK LQDYTKSEII YESLQIQDIR
610 620 630 640 650
KLEEQNAVDF WVMALPNKVC EPFVETIQSV HGKSKIIDLS ADHRFVSESD
660 670 680 690 700
WAYGLPELND RAKIANAAKI ANPGCYATGS QLTISPLTKY INGLPTVFGV
710 720 730 740 750
SGYSGAGTKP SPKNDPKFLN NNLIPYALSD HIHEREISAR IGHNVAFMPH
760 770 780 790 800
VGQWFQGISL TVSIPIKKGS LSIDEIRKLY RNFYEDEKLV HVIDDIPLVK
810 820 830 840 850
DIEGTHGVVI GGFKLNDAED RVVVCATIDN LLKGAATQCL QNINLAMGYG
860
EYAGIPENKI IGV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57017 Genomic DNA Translation: CAA40336.1 U18813 Genomic DNA Translation: AAB64605.1 BK006939 Genomic DNA Translation: DAA07728.1 |
PIRi | S16807 |
RefSeqi | NP_010992.1, NM_001178960.1 |
Genome annotation databases
EnsemblFungii | YER069W_mRNA; YER069W; YER069W |
GeneIDi | 856800 |
KEGGi | sce:YER069W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X57017 Genomic DNA Translation: CAA40336.1 U18813 Genomic DNA Translation: AAB64605.1 BK006939 Genomic DNA Translation: DAA07728.1 |
PIRi | S16807 |
RefSeqi | NP_010992.1, NM_001178960.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ZZF | X-ray | 2.20 | A/B/C/D | 58-356 | [»] | |
3ZZG | X-ray | 2.95 | A/B/C/D | 58-356 | [»] | |
3ZZH | X-ray | 2.10 | A/B/C/D | 58-356 | [»] | |
3ZZI | X-ray | 3.80 | A/B/C/D/E/F/G/H | 58-513 | [»] | |
4AB7 | X-ray | 3.25 | A/B/C/D/E/F/G/H | 58-513 | [»] | |
SMRi | Q01217 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36812, 23 interactors |
ComplexPortali | CPX-1151, N-acetylglutamate synthase NAGS/NAGK complex |
DIPi | DIP-5348N |
IntActi | Q01217, 3 interactors |
MINTi | Q01217 |
STRINGi | 4932.YER069W |
Protein family/group databases
MoonProti | Q01217 |
PTM databases
iPTMneti | Q01217 |
Proteomic databases
MaxQBi | Q01217 |
PaxDbi | Q01217 |
PRIDEi | Q01217 |
Genome annotation databases
EnsemblFungii | YER069W_mRNA; YER069W; YER069W |
GeneIDi | 856800 |
KEGGi | sce:YER069W |
Organism-specific databases
EuPathDBi | FungiDB:YER069W |
SGDi | S000000871, ARG5,6 |
Phylogenomic databases
eggNOGi | KOG2436, Eukaryota KOG4354, Eukaryota |
HOGENOMi | CLU_006384_4_0_1 |
InParanoidi | Q01217 |
OMAi | TSRDIRN |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00107 UPA00068;UER00108 |
BRENDAi | 2.7.2.8, 984 |
Reactomei | R-SCE-70635, Urea cycle |
Miscellaneous databases
PROi | PR:Q01217 |
RNActi | Q01217, protein |
Family and domain databases
CDDi | cd04252, AAK_NAGK-fArgBP, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit |
HAMAPi | MF_00150, ArgC_type1, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR004662, AcgluKinase_fam IPR023013, AGPR_AS IPR000706, AGPR_type-1 IPR001048, Asp/Glu/Uridylate_kinase IPR036291, NAD(P)-bd_dom_sf IPR041734, NAGK-fArgBP IPR011241, NAGK/NAGSA IPR000534, Semialdehyde_DH_NAD-bd IPR012280, Semialdhyde_DH_dimer_dom IPR006855, Vertebrate-like_GNAT_dom |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF04768, NAT, 1 hit PF01118, Semialdhyde_dh, 1 hit PF02774, Semialdhyde_dhC, 1 hit |
PIRSFi | PIRSF036440, ARG5-6, 1 hit |
SMARTi | View protein in SMART SM00859, Semialdhyde_dh, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF53633, SSF53633, 1 hit |
TIGRFAMsi | TIGR00761, argB, 1 hit TIGR01850, argC, 1 hit |
PROSITEi | View protein in PROSITE PS01224, ARGC, 1 hit PS51731, GNAT_NAGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARG56_YEAST | |
Accessioni | Q01217Primary (citable) accession number: Q01217 Secondary accession number(s): D3DLX4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 199 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome V
Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD