UniProtKB - Q01147 (CREB1_MOUSE)
Cyclic AMP-responsive element-binding protein 1
Creb1
Functioni
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 300 | Required for binding TORCsBy similarity | 1 |
GO - Molecular functioni
- arrestin family protein binding Source: MGI
- cAMP response element binding Source: ARUK-UCL
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: ARUK-UCL
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- double-stranded DNA binding Source: MGI
- enzyme binding Source: MGI
- histone acetyltransferase binding Source: MGI
- Hsp70 protein binding Source: MGI
- identical protein binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription cis-regulatory region binding Source: MGI
- transcription coactivator binding Source: MGI
GO - Biological processi
- axonogenesis Source: MGI
- cellular response to growth factor stimulus Source: BHF-UCL
- cellular response to hepatocyte growth factor stimulus Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- cellular response to retinoic acid Source: ARUK-UCL
- cellular response to zinc ion Source: MGI
- chemotaxis to arachidonic acid Source: MGI
- circadian rhythm Source: UniProtKB
- lactation Source: MGI
- lung epithelium development Source: MGI
- lung saccule development Source: MGI
- mammary gland development Source: MGI
- memory Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of neuron death Source: MGI
- negative regulation of transcription by competitive promoter binding Source: MGI
- pituitary gland development Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of cardiac muscle tissue development Source: MGI
- positive regulation of fat cell differentiation Source: UniProtKB
- positive regulation of hormone secretion Source: MGI
- positive regulation of lipid biosynthetic process Source: UniProtKB
- positive regulation of long-term synaptic potentiation Source: MGI
- positive regulation of multicellular organism growth Source: MGI
- positive regulation of osteoclast differentiation Source: MGI
- positive regulation of RNA polymerase II transcription preinitiation complex assembly Source: MGI
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of transforming growth factor beta3 production Source: MGI
- protein phosphorylation Source: MGI
- protein stabilization Source: UniProtKB
- regulation of apoptotic process Source: MGI
- regulation of cell size Source: MGI
- regulation of circadian rhythm Source: MGI
- regulation of fibroblast proliferation Source: MGI
- regulation of glial cell proliferation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: ComplexPortal
- response to glucagon Source: UniProtKB
- response to organic substance Source: MGI
- response to xenobiotic stimulus Source: MGI
- secretory granule organization Source: MGI
- transcription by RNA polymerase II Source: MGI
- transforming growth factor beta receptor signaling pathway Source: MGI
- type I pneumocyte differentiation Source: MGI
- visual learning Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Biological rhythms, Differentiation, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-198693, AKT phosphorylates targets in the nucleus R-MMU-199920, CREB phosphorylation R-MMU-375165, NCAM signaling for neurite out-growth R-MMU-9031628, NGF-stimulated transcription R-MMU-9634638, Estrogen-dependent nuclear events downstream of ESR-membrane signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclic AMP-responsive element-binding protein 1Short name: CREB-1 Short name: cAMP-responsive element-binding protein 1 |
Gene namesi | Name:Creb1 Synonyms:Creb-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88494, Creb1 |
VEuPathDBi | HostDB:ENSMUSG00000025958 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Mitochondrion
- mitochondrial matrix Source: MGI
- mitochondrion Source: MGI
Nucleus
- ATF4-CREB1 transcription factor complex Source: ParkinsonsUK-UCL
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- RNA polymerase II transcription regulator complex Source: ComplexPortal
Other locations
- axon Source: MGI
- chromatin Source: BHF-UCL
- euchromatin Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 119 | S → A: Loss of phosphorylation by RPS6KA4 and RPS6KA5. 1 Publication | 1 | |
Mutagenesisi | 123 – 124 | IL → AA: Abolishes CREBBP binding. 1 Publication | 2 | |
Mutagenesisi | 128 | S → A: Attenuates light-induced phase shifts of locomotion and expression of c-Fos and mPer1 in the SCN. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076598 | 1 – 327 | Cyclic AMP-responsive element-binding protein 1Add BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 119 | Phosphoserine; by CaMK1, CaMK2, CaMK4, PKB/AKT1 or PKB/AKT2, RPS6KA3, RPS6KA4, RPS6KA5 and SGK1PROSITE-ProRule annotation3 Publications | 1 | |
Cross-linki | 122 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 128 | Phosphoserine; by CaMK2PROSITE-ProRule annotationBy similarity | 1 | |
Modified residuei | 257 | Phosphoserine; by HIPK2PROSITE-ProRule annotationBy similarity | 1 | |
Cross-linki | 271 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity | ||
Cross-linki | 290 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q01147 |
jPOSTi | Q01147 |
PaxDbi | Q01147 |
PeptideAtlasi | Q01147 |
PRIDEi | Q01147 |
ProteomicsDBi | 284119 [Q01147-2] 284120 [Q01147-2] |
PTM databases
iPTMneti | Q01147 |
PhosphoSitePlusi | Q01147 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000025958, Expressed in mesenteric lymph node and 305 other tissues |
Genevisiblei | Q01147, MM |
Interactioni
Subunit structurei
Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions.
Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3.
Interacts (phosphorylated form) with TOX3 (By similarity). When phosphorylated on Ser-119, binds CREBBP.
Interacts with ARRB1. Binds to HIPK2 (By similarity).
Interacts with SGK1 (By similarity).
Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity.
Interacts with TSSK4; this interaction facilitates phosphorylation on Ser-119 (By similarity).
Forms a complex with KMT2A and CREBBP (By similarity).
By similarity3 PublicationsBinary interactionsi
GO - Molecular functioni
- arrestin family protein binding Source: MGI
- enzyme binding Source: MGI
- histone acetyltransferase binding Source: MGI
- Hsp70 protein binding Source: MGI
- identical protein binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
- transcription coactivator binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198873, 27 interactors |
ComplexPortali | CPX-6, bZIP transcription factor complex, Atf4-Creb1 |
CORUMi | Q01147 |
IntActi | Q01147, 10 interactors |
MINTi | Q01147 |
STRINGi | 10090.ENSMUSP00000140112 |
Miscellaneous databases
RNActi | Q01147, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q01147 |
SMRi | Q01147 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q01147 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 87 – 146 | KIDPROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 269 – 327 | bZIPPROSITE-ProRule annotationAdd BLAST | 59 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 27 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 94 – 113 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 126 – 151 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 270 – 295 | Basic motifPROSITE-ProRule annotationAdd BLAST | 26 | |
Regioni | 297 – 318 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3584, Eukaryota |
GeneTreei | ENSGT00940000155408 |
InParanoidi | Q01147 |
OMAi | SVIHTTQ |
PhylomeDBi | Q01147 |
TreeFami | TF106464 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR003102, Coactivator_CBP_pKID IPR029802, CREB1 IPR001630, Leuzip_CREB |
PANTHERi | PTHR45879, PTHR45879, 1 hit PTHR45879:SF1, PTHR45879:SF1, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit PF02173, pKID, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit PS50953, KID, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTMESGADNQ QSGDAAVTEA ENQQMTVQAQ PQIATLAQVS MPAAHATSSA
60 70 80 90 100
PTVTLVQLPN GQTVQVHGVI QAAQPSVIQS PQVQTVQIST IAESEDSQES
110 120 130 140 150
VDSVTDSQKR REILSRRPSY RKILNDLSSD APGVPRIEEE KSEEETSAPA
160 170 180 190 200
ITTVTVPTPI YQTSSGQYIA ITQGGAIQLA NNGTDGVQGL QTLTMTNAAA
210 220 230 240 250
TQPGTTILQY AQTTDGQQIL VPSNQVVVQA ASGDVQTYQI RTAPTSTIAP
260 270 280 290 300
GVVMASSPAL PTQPAEEAAR KREVRLMKNR EAARECRRKK KEYVKCLENR
310 320
VAVLENQNKT LIEELKALKD LYCHKSD
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ62347 | Q62347_MOUSE | Cyclic AMP-responsive element-bindi... | Creb1 | 287 | Annotation score: | ||
Q61441 | Q61441_MOUSE | C-AMP-responsive-element binding pr... | Creb1 CREB | 90 | Annotation score: | ||
A0A087WRI6 | A0A087WRI6_MOUSE | Cyclic AMP-responsive element-bindi... | Creb1 | 317 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060704 | 86 | V → VQSSCKDLKRLFSGT in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67719 , X67721, X67724, X67725, X67726, X67727, X67728 Genomic DNA Translation: CAA47953.1 X67719 , X67721, X67722, X67724, X67725, X67726, X67727, X67728 Genomic DNA Translation: CAA47954.1 M95106 mRNA Translation: AAA37456.1 |
CCDSi | CCDS15004.1 [Q01147-1] CCDS15005.1 [Q01147-2] |
PIRi | S20955 S42699 |
RefSeqi | NP_034082.1, NM_009952.2 [Q01147-1] NP_598589.2, NM_133828.2 [Q01147-2] XP_006495713.1, XM_006495650.3 [Q01147-1] XP_006495714.1, XM_006495651.3 [Q01147-2] XP_017169569.1, XM_017314080.1 [Q01147-1] XP_017169588.1, XM_017314099.1 [Q01147-2] |
Genome annotation databases
Ensembli | ENSMUST00000049932; ENSMUSP00000059973; ENSMUSG00000025958 [Q01147-1] ENSMUST00000087366; ENSMUSP00000084624; ENSMUSG00000025958 [Q01147-2] ENSMUST00000185594; ENSMUSP00000139995; ENSMUSG00000025958 [Q01147-2] ENSMUST00000190348; ENSMUSP00000140112; ENSMUSG00000025958 [Q01147-1] |
GeneIDi | 12912 |
KEGGi | mmu:12912 |
UCSCi | uc007bgr.1, mouse [Q01147-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X67719 , X67721, X67724, X67725, X67726, X67727, X67728 Genomic DNA Translation: CAA47953.1 X67719 , X67721, X67722, X67724, X67725, X67726, X67727, X67728 Genomic DNA Translation: CAA47954.1 M95106 mRNA Translation: AAA37456.1 |
CCDSi | CCDS15004.1 [Q01147-1] CCDS15005.1 [Q01147-2] |
PIRi | S20955 S42699 |
RefSeqi | NP_034082.1, NM_009952.2 [Q01147-1] NP_598589.2, NM_133828.2 [Q01147-2] XP_006495713.1, XM_006495650.3 [Q01147-1] XP_006495714.1, XM_006495651.3 [Q01147-2] XP_017169569.1, XM_017314080.1 [Q01147-1] XP_017169588.1, XM_017314099.1 [Q01147-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DH3 | X-ray | 3.00 | A/C | 271-325 | [»] | |
BMRBi | Q01147 | |||||
SMRi | Q01147 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198873, 27 interactors |
ComplexPortali | CPX-6, bZIP transcription factor complex, Atf4-Creb1 |
CORUMi | Q01147 |
IntActi | Q01147, 10 interactors |
MINTi | Q01147 |
STRINGi | 10090.ENSMUSP00000140112 |
PTM databases
iPTMneti | Q01147 |
PhosphoSitePlusi | Q01147 |
Proteomic databases
EPDi | Q01147 |
jPOSTi | Q01147 |
PaxDbi | Q01147 |
PeptideAtlasi | Q01147 |
PRIDEi | Q01147 |
ProteomicsDBi | 284119 [Q01147-2] 284120 [Q01147-2] |
Protocols and materials databases
ABCDi | Q01147, 1 sequenced antibody |
Antibodypediai | 3536, 2220 antibodies from 54 providers |
DNASUi | 12912 |
Genome annotation databases
Ensembli | ENSMUST00000049932; ENSMUSP00000059973; ENSMUSG00000025958 [Q01147-1] ENSMUST00000087366; ENSMUSP00000084624; ENSMUSG00000025958 [Q01147-2] ENSMUST00000185594; ENSMUSP00000139995; ENSMUSG00000025958 [Q01147-2] ENSMUST00000190348; ENSMUSP00000140112; ENSMUSG00000025958 [Q01147-1] |
GeneIDi | 12912 |
KEGGi | mmu:12912 |
UCSCi | uc007bgr.1, mouse [Q01147-2] |
Organism-specific databases
CTDi | 1385 |
MGIi | MGI:88494, Creb1 |
VEuPathDBi | HostDB:ENSMUSG00000025958 |
Phylogenomic databases
eggNOGi | KOG3584, Eukaryota |
GeneTreei | ENSGT00940000155408 |
InParanoidi | Q01147 |
OMAi | SVIHTTQ |
PhylomeDBi | Q01147 |
TreeFami | TF106464 |
Enzyme and pathway databases
Reactomei | R-MMU-198693, AKT phosphorylates targets in the nucleus R-MMU-199920, CREB phosphorylation R-MMU-375165, NCAM signaling for neurite out-growth R-MMU-9031628, NGF-stimulated transcription R-MMU-9634638, Estrogen-dependent nuclear events downstream of ESR-membrane signaling |
Miscellaneous databases
BioGRID-ORCSi | 12912, 11 hits in 67 CRISPR screens |
ChiTaRSi | Creb1, mouse |
EvolutionaryTracei | Q01147 |
PROi | PR:Q01147 |
RNActi | Q01147, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025958, Expressed in mesenteric lymph node and 305 other tissues |
Genevisiblei | Q01147, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR003102, Coactivator_CBP_pKID IPR029802, CREB1 IPR001630, Leuzip_CREB |
PANTHERi | PTHR45879, PTHR45879, 1 hit PTHR45879:SF1, PTHR45879:SF1, 1 hit |
Pfami | View protein in Pfam PF00170, bZIP_1, 1 hit PF02173, pKID, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit PS00036, BZIP_BASIC, 1 hit PS50953, KID, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CREB1_MOUSE | |
Accessioni | Q01147Primary (citable) accession number: Q01147 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | October 7, 2020 | |
Last modified: | February 23, 2022 | |
This is version 211 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families