Insulinoma-associated protein 1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• chromatin DNA binding Source: UniProtKB <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.
• cyclin binding Source: UniProtKB <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.
  • Ref.9

    "Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest."
    Zhang T., Liu W.D., Saunee N.A., Breslin M.B., Lan M.S.
    J. Biol. Chem. 284:5574-5581(2009) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1, MUTAGENESIS OF 43-PRO--PRO-58.
• DNA-binding transcription factor activity Source: UniProtKBInferred from direct assayⁱ
  • Ref.4

    "Neuroendocrine differentiation factor, IA-1, is a transcriptional repressor and contains a specific DNA-binding domain: identification of consensus IA-1 binding sequence."
    Breslin M.B., Zhu M., Notkins A.L., Lan M.S.
    Nucleic Acids Res. 30:1038-1045(2002) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING.
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.
• DNA-binding transcription repressor activity, RNA polymerase II-specific Source: NTNU_SBInferred from direct assayⁱ
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
• histone deacetylase binding Source: UniProtKBInferred from physical interactionⁱ
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.
• metal ion binding Source: UniProtKB-KW
• RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKBInferred from direct assayⁱ
  • Ref.4

    "Neuroendocrine differentiation factor, IA-1, is a transcriptional repressor and contains a specific DNA-binding domain: identification of consensus IA-1 binding sequence."
    Breslin M.B., Zhu M., Notkins A.L., Lan M.S.
    Nucleic Acids Res. 30:1038-1045(2002) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.

GO - Biological processⁱ

• adrenal chromaffin cell differentiation Source: UniProtKB
• cell cycle Source: UniProtKB-KW
• endocrine pancreas development Source: Reactome
• negative regulation of cell population proliferation Source: UniProtKBInferred from direct assayⁱ
  • Ref.9

    "Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest."
    Zhang T., Liu W.D., Saunee N.A., Breslin M.B., Lan M.S.
    J. Biol. Chem. 284:5574-5581(2009) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1, MUTAGENESIS OF 43-PRO--PRO-58.
• negative regulation of protein phosphorylation Source: UniProtKBInferred from direct assayⁱ
  • Ref.9

    "Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest."
    Zhang T., Liu W.D., Saunee N.A., Breslin M.B., Lan M.S.
    J. Biol. Chem. 284:5574-5581(2009) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1, MUTAGENESIS OF 43-PRO--PRO-58.
• negative regulation of transcription by RNA polymerase II Source: UniProtKBInferred from direct assayⁱ
  • Ref.4

    "Neuroendocrine differentiation factor, IA-1, is a transcriptional repressor and contains a specific DNA-binding domain: identification of consensus IA-1 binding sequence."
    Breslin M.B., Zhu M., Notkins A.L., Lan M.S.
    Nucleic Acids Res. 30:1038-1045(2002) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING.
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.
  • Ref.9

    "Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest."
    Zhang T., Liu W.D., Saunee N.A., Breslin M.B., Lan M.S.
    J. Biol. Chem. 284:5574-5581(2009) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1, MUTAGENESIS OF 43-PRO--PRO-58.
• neuron differentiation Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• noradrenergic neuron development Source: UniProtKB
• norepinephrine biosynthetic process Source: UniProtKB
• pancreatic A cell differentiation Source: UniProtKB
• positive regulation of cell cycle arrest Source: UniProtKBInferred from direct assayⁱ
  • Ref.9

    "Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest."
    Zhang T., Liu W.D., Saunee N.A., Breslin M.B., Lan M.S.
    J. Biol. Chem. 284:5574-5581(2009) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1, MUTAGENESIS OF 43-PRO--PRO-58.
• positive regulation of cell differentiation Source: UniProtKB
• positive regulation of cell migration Source: Ensembl
• positive regulation of neural precursor cell proliferation Source: UniProtKB
• regulation of cell cycle process Source: GO_CentralInferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• regulation of gene expression Source: UniProtKB
• regulation of protein-containing complex assembly Source: UniProtKBInferred from direct assayⁱ
  • Ref.9

    "Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest."
    Zhang T., Liu W.D., Saunee N.A., Breslin M.B., Lan M.S.
    J. Biol. Chem. 284:5574-5581(2009) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1, MUTAGENESIS OF 43-PRO--PRO-58.
• sympathetic ganglion development Source: UniProtKB
• transdifferentiation Source: UniProtKBInferred from direct assayⁱ
  • Ref.7

    "IA1 is NGN3-dependent and essential for differentiation of the endocrine pancreas."
    Mellitzer G., Bonne S., Luco R.F., Van De Casteele M., Lenne-Samuel N., Collombat P., Mansouri A., Lee J., Lan M., Pipeleers D., Nielsen F.C., Ferrer J., Gradwohl G., Heimberg H.
    EMBO J. 25:1344-1352(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, INDUCTION.
• type B pancreatic cell development Source: Ensembl
• type B pancreatic cell differentiation Source: UniProtKB

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

Mutagenesis

Organism-specific databases

Miscellaneous databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityⁱ

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductionⁱ

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

GO - Molecular functionⁱ

• cyclin binding Source: UniProtKBInferred from physical interactionⁱ
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.
  • Ref.9

    "Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest."
    Zhang T., Liu W.D., Saunee N.A., Breslin M.B., Lan M.S.
    J. Biol. Chem. 284:5574-5581(2009) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1, MUTAGENESIS OF 43-PRO--PRO-58.
• histone deacetylase binding Source: UniProtKBInferred from physical interactionⁱ
  • Ref.6

    "INSM1 functions as a transcriptional repressor of the neuroD/beta2 gene through the recruitment of cyclin D1 and histone deacetylases."
    Liu W.D., Wang H.W., Muguira M., Breslin M.B., Lan M.S.
    Biochem. J. 397:169-177(2006) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1; HDAC1 AND HDAC3.
  • Ref.8

    "Identification of an INSM1-binding site in the insulin promoter: negative regulation of the insulin gene transcription."
    Wang H.W., Muguira M., Liu W.D., Zhang T., Chen C., Aucoin R., Breslin M.B., Lan M.S.
    J. Endocrinol. 198:29-39(2008) [PubMed] [Europe PMC] [Abstract]

    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CCND1 AND HDAC3.

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Region

Compositional bias

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Zinc finger

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MPRGFLVKRS KKSTPVSYRV RGGEDGDRAL LLSPSCGGAR AEPPAPSPVP 
        60         70         80         90        100
GPLPPPPPAE RAHAALAAAL ACAPGPQPPP QGPRAAHFGN PEAAHPAPLY 
       110        120        130        140        150
SPTRPVSREH EKHKYFERSF NLGSPVSAES FPTPAALLGG GGGGGASGAG 
       160        170        180        190        200
GGGTCGGDPL LFAPAELKMG TAFSAGAEAA RGPGPGPPLP PAAALRPPGK 
       210        220        230        240        250
RPPPPTAAEP PAKAVKAPGA KKPKAIRKLH FEDEVTTSPV LGLKIKEGPV 
       260        270        280        290        300
EAPRGRAGGA ARPLGEFICQ LCKEEYADPF ALAQHKCSRI VRVEYRCPEC 
       310        320        330        340        350
AKVFSCPANL ASHRRWHKPR PAPAAARAPE PEAAARAEAR EAPGGGSDRD 
       360        370        380        390        400
TPSPGGVSES GSEDGLYECH HCAKKFRRQA YLRKHLLAHH QALQAKGAPL 
       410        420        430        440        450
APPAEDLLAL YPGPDEKAPQ EAAGDGEGAG VLGLSASAEC HLCPVCGESF 
       460        470        480        490        500
ASKGAQERHL RLLHAAQVFP CKYCPATFYS SPGLTRHINK CHPSENRQVI 
       510
LLQVPVRPAC                                             

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
2. SIMILARITY comments
   Index of protein domains and families
3. MIM cross-references
   Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
4. Human chromosome 20
   Human chromosome 20: entries, gene names and cross-references to MIM