Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein spitz

Gene

spi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for the EGF receptor (Gurken). Involved in a number of unrelated developmental choices, for example, dorsal-ventral axis formation, glial migration, sensory organ determination, and muscle development. It is required for photoreceptor determination.1 Publication

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: FlyBase

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Neurogenesis

Enzyme and pathway databases

SignaLinkiQ01083

Names & Taxonomyi

Protein namesi
Recommended name:
Protein spitz
Gene namesi
Name:spi
ORF Names:CG10334
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0005672 spi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 143LumenalSequence analysisAdd BLAST115
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 234CytoplasmicSequence analysisAdd BLAST70

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000000773729 – 234Protein spitzAdd BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi74N-linked (GlcNAc...) asparagine1
Disulfide bondi82 ↔ 97PROSITE-ProRule annotation1 Publication
Disulfide bondi91 ↔ 110PROSITE-ProRule annotation1 Publication
Disulfide bondi112 ↔ 121PROSITE-ProRule annotation1 Publication

Post-translational modificationi

Proteolytic processing by Rhomboid occurs in the Golgi. Cleavage takes place within the transmembrane domain close to residue 144 and the active growth factor is released.1 Publication
N-glycosylated and O-glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ01083
PRIDEiQ01083

PTM databases

SwissPalmiQ01083

Miscellaneous databases

PMAP-CutDBiQ01083

Expressioni

Tissue specificityi

Expressed throughout the embryo.

Gene expression databases

BgeeiFBgn0005672 Expressed in 49 organ(s), highest expression level in embryo
ExpressionAtlasiQ01083 baseline and differential
GenevisibleiQ01083 DM

Interactioni

Subunit structurei

Interacts with Star via the lumenal domain.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi61229, 72 interactors
DIPiDIP-18037N
IntActiQ01083, 6 interactors
STRINGi7227.FBpp0080808

Structurei

Secondary structure

1234
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ01083
SMRiQ01083
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01083

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 122EGF-likePROSITE-ProRule annotationAdd BLAST45

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi65 – 70Poly-Thr6
Compositional biasi186 – 189Poly-Asp4

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYKD Eukaryota
ENOG4111N7J LUCA
GeneTreeiENSGT00520000062215
InParanoidiQ01083
KOiK20229
OMAiEHALMSF
OrthoDBiEOG091G0XGN
PhylomeDBiQ01083

Family and domain databases

InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
SMARTiView protein in SMART
SM00181 EGF, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q01083-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHSTMSVQHG LVALVLIGCL AHPWHVEACS SRTVPKPRSS ISSSMSGTAL
60 70 80 90 100
PPTQAPVTSS TTMRTTTTTT PRPNITFPTY KCPETFDAWY CLNDAHCFAV
110 120 130 140 150
KIADLPVYSC ECAIGFMGQR CEYKEIDNTY LPKRPRPMLE KASIASGAMC
160 170 180 190 200
ALVFMLFVCL AFYLRFEQRA AKKAYELEQE LQQEYDDDDG QCECCRNRCC
210 220 230
PDGQEPVILE RKLPYHMRLE HALMSFAIRR SNKL
Length:234
Mass (Da):26,424
Last modified:July 19, 2004 - v2
Checksum:i237F7A3B03DBD685
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A4V0W1A4V0W1_DROME
FI04747p
spi anon-WO0118547.158, CT29014, Dmel\CG10334, EP(2)2378, l(2)01068 spi anon-WO0118547.158, CT29014, Dmel\CG10334, EP(2)2378, l(2)01068, l(2)37Fa, l(2)s3547, s-spi, Spi, spi-RF, Spitz, sSpi, CG10334, Dmel_CG10334
234Annotation score:

Sequence cautioni

The sequence AAA28894 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119Q → R in AAL90431 (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95199 mRNA Translation: AAA28894.1 Different initiation.
AE014134 Genomic DNA Translation: AAF53831.2
AY089693 mRNA Translation: AAL90431.1
PIRiA44074
RefSeqiNP_001027281.1, NM_001032110.2
NP_476909.2, NM_057561.6
NP_599118.2, NM_134291.5
NP_599119.2, NM_134292.3
NP_599120.2, NM_134293.3
UniGeneiDm.4573

Genome annotation databases

EnsemblMetazoaiFBtr0081267; FBpp0080808; FBgn0005672
FBtr0081268; FBpp0080809; FBgn0005672
FBtr0081269; FBpp0080810; FBgn0005672
FBtr0081270; FBpp0080811; FBgn0005672
FBtr0100597; FBpp0100054; FBgn0005672
GeneIDi35253
KEGGidme:Dmel_CG10334
UCSCiCG10334-RA d. melanogaster

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95199 mRNA Translation: AAA28894.1 Different initiation.
AE014134 Genomic DNA Translation: AAF53831.2
AY089693 mRNA Translation: AAL90431.1
PIRiA44074
RefSeqiNP_001027281.1, NM_001032110.2
NP_476909.2, NM_057561.6
NP_599118.2, NM_134291.5
NP_599119.2, NM_134292.3
NP_599120.2, NM_134293.3
UniGeneiDm.4573

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C9AX-ray1.60C/D76-127[»]
3CA7X-ray1.50A76-127[»]
3LTFX-ray3.20B/D76-133[»]
3LTGX-ray3.40D76-126[»]
ProteinModelPortaliQ01083
SMRiQ01083
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61229, 72 interactors
DIPiDIP-18037N
IntActiQ01083, 6 interactors
STRINGi7227.FBpp0080808

PTM databases

SwissPalmiQ01083

Proteomic databases

PaxDbiQ01083
PRIDEiQ01083

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081267; FBpp0080808; FBgn0005672
FBtr0081268; FBpp0080809; FBgn0005672
FBtr0081269; FBpp0080810; FBgn0005672
FBtr0081270; FBpp0080811; FBgn0005672
FBtr0100597; FBpp0100054; FBgn0005672
GeneIDi35253
KEGGidme:Dmel_CG10334
UCSCiCG10334-RA d. melanogaster

Organism-specific databases

CTDi35253
FlyBaseiFBgn0005672 spi

Phylogenomic databases

eggNOGiENOG410IYKD Eukaryota
ENOG4111N7J LUCA
GeneTreeiENSGT00520000062215
InParanoidiQ01083
KOiK20229
OMAiEHALMSF
OrthoDBiEOG091G0XGN
PhylomeDBiQ01083

Enzyme and pathway databases

SignaLinkiQ01083

Miscellaneous databases

ChiTaRSispi fly
EvolutionaryTraceiQ01083
GenomeRNAii35253
PMAP-CutDBiQ01083
PROiPR:Q01083

Gene expression databases

BgeeiFBgn0005672 Expressed in 49 organ(s), highest expression level in embryo
ExpressionAtlasiQ01083 baseline and differential
GenevisibleiQ01083 DM

Family and domain databases

InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
SMARTiView protein in SMART
SM00181 EGF, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPITZ_DROME
AccessioniPrimary (citable) accession number: Q01083
Secondary accession number(s): Q8SXE0, Q9VIT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 19, 2004
Last modified: September 12, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again