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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

PDE1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.1 Publication

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cation1 PublicationNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.1 Publication

Activity regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei223Proton donorBy similarity1
Metal bindingi227Divalent metal cation 1; via tele nitrogenCombined sources1 Publication1
Metal bindingi263Divalent metal cation 1; via tele nitrogenCombined sources1 Publication1
Metal bindingi264Divalent metal cation 1Combined sources1 Publication1
Metal bindingi264Divalent metal cation 2Combined sources1 Publication1
Metal bindingi370Divalent metal cation 1Combined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
LigandcAMP, cGMP, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.17 2681
ReactomeiR-HSA-111957 Cam-PDE 1 activation
R-HSA-418457 cGMP effects
R-HSA-418555 G alpha (s) signalling events
SIGNORiQ01064

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
Short name:
Cam-PDE 1B
Alternative name(s):
63 kDa Cam-PDE
Gene namesi
Name:PDE1B
Synonyms:PDE1B1, PDES1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000123360.11
HGNCiHGNC:8775 PDE1B
MIMi171891 gene
neXtProtiNX_Q01064

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi5153
OpenTargetsiENSG00000123360
PharmGKBiPA33123

Chemistry databases

ChEMBLiCHEMBL4425
DrugBankiDB01244 Bepridil
DB00201 Caffeine
DB01023 Felodipine
DB00622 Nicardipine
GuidetoPHARMACOLOGYi1295

Polymorphism and mutation databases

BioMutaiPDE1B
DMDMi3183514

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987891 – 536Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BAdd BLAST536

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei466PhosphoserineBy similarity1
Modified residuei514PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ01064
PaxDbiQ01064
PeptideAtlasiQ01064
PRIDEiQ01064
ProteomicsDBi57904
57905 [Q01064-2]

PTM databases

iPTMnetiQ01064
PhosphoSitePlusiQ01064

Expressioni

Gene expression databases

BgeeiENSG00000123360 Expressed in 109 organ(s), highest expression level in caudate nucleus
CleanExiHS_PDE1B
ExpressionAtlasiQ01064 baseline and differential
GenevisibleiQ01064 HS

Organism-specific databases

HPAiHPA018492

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
POT1Q9NUX52EBI-7413304,EBI-752420

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111179, 3 interactors
IntActiQ01064, 2 interactors
MINTiQ01064
STRINGi9606.ENSP00000243052

Chemistry databases

BindingDBiQ01064

Structurei

Secondary structure

1536
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ01064
SMRiQ01064
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ01064

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 503PDEasePROSITE-ProRule annotationAdd BLAST358

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 47Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3688 Eukaryota
ENOG410XQDD LUCA
GeneTreeiENSGT00760000118889
HOGENOMiHOG000231888
HOVERGENiHBG056120
KOiK13755
OMAiTRHTEDN
OrthoDBiEOG091G04C4
PhylomeDBiQ01064
TreeFamiTF314638

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR023088 PDEase
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
IPR013706 PDEase_N
PfamiView protein in Pfam
PF00233 PDEase_I, 1 hit
PF08499 PDEase_I_N, 1 hit
PRINTSiPR00387 PDIESTERASE1
SMARTiView protein in SMART
SM00471 HDc, 1 hit
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform PDE1B1 (identifier: Q01064-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELSPRSPPE MLEESDCPSP LELKSAPSKK MWIKLRSLLR YMVKQLENGE
60 70 80 90 100
INIEELKKNL EYTASLLEAV YIDETRQILD TEDELQELRS DAVPSEVRDW
110 120 130 140 150
LASTFTQQAR AKGRRAEEKP KFRSIVHAVQ AGIFVERMFR RTYTSVGPTY
160 170 180 190 200
STAVLNCLKN LDLWCFDVFS LNQAADDHAL RTIVFELLTR HNLISRFKIP
210 220 230 240 250
TVFLMSFLDA LETGYGKYKN PYHNQIHAAD VTQTVHCFLL RTGMVHCLSE
260 270 280 290 300
IELLAIIFAA AIHDYEHTGT TNSFHIQTKS ECAIVYNDRS VLENHHISSV
310 320 330 340 350
FRLMQDDEMN IFINLTKDEF VELRALVIEM VLATDMSCHF QQVKTMKTAL
360 370 380 390 400
QQLERIDKPK ALSLLLHAAD ISHPTKQWLV HSRWTKALME EFFRQGDKEA
410 420 430 440 450
ELGLPFSPLC DRTSTLVAQS QIGFIDFIVE PTFSVLTDVA EKSVQPLADE
460 470 480 490 500
DSKSKNQPSF QWRQPSLDVE VGDPNPDVVS FRSTWVKRIQ ENKQKWKERA
510 520 530
ASGITNQMSI DELSPCEEEA PPSPAEDEHN QNGNLD
Length:536
Mass (Da):61,380
Last modified:July 15, 1998 - v2
Checksum:iD15211AC32C756A4
GO
Isoform PDE1B2 (identifier: Q01064-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MELSPRSPPEMLEESDCP → MANPVPVQRSHLQGPILR
     19-38: Missing.

Show »
Length:516
Mass (Da):59,051
Checksum:i0A9093462479DC5C
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B4DK72B4DK72_HUMAN
Phosphodiesterase
PDE1B
495Annotation score:
F8VZK3F8VZK3_HUMAN
Calcium/calmodulin-dependent 3',5'-...
PDE1B
191Annotation score:
A0A087WTW8A0A087WTW8_HUMAN
Calcium/calmodulin-dependent 3',5'-...
PDE1B
50Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225 – 226QI → SM in AAA58405 (PubMed:9419816).Curated2
Sequence conflicti337S → A in AAA58405 (PubMed:9419816).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0386431 – 18MELSP…ESDCP → MANPVPVQRSHLQGPILR in isoform PDE1B2. 2 PublicationsAdd BLAST18
Alternative sequenceiVSP_03864419 – 38Missing in isoform PDE1B2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56976 mRNA Translation: AAC50769.1
U86078 mRNA Translation: AAC51872.1
AJ401609 mRNA Translation: CAC82207.1
AK302931 mRNA Translation: BAG64092.1
CH471054 Genomic DNA Translation: EAW96790.1
BC032226 mRNA Translation: AAH32226.1
M94539 mRNA Translation: AAA58405.1
CCDSiCCDS53800.1 [Q01064-2]
CCDS8882.1 [Q01064-1]
PIRiJC6129
RefSeqiNP_000915.1, NM_000924.3 [Q01064-1]
NP_001159447.1, NM_001165975.2 [Q01064-2]
NP_001275697.1, NM_001288768.1
NP_001275698.1, NM_001288769.1
NP_001302463.1, NM_001315534.1
NP_001302464.1, NM_001315535.1
UniGeneiHs.530871

Genome annotation databases

EnsembliENST00000243052; ENSP00000243052; ENSG00000123360 [Q01064-1]
ENST00000550620; ENSP00000448519; ENSG00000123360 [Q01064-2]
GeneIDi5153
KEGGihsa:5153
UCSCiuc001sgd.3 human [Q01064-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56976 mRNA Translation: AAC50769.1
U86078 mRNA Translation: AAC51872.1
AJ401609 mRNA Translation: CAC82207.1
AK302931 mRNA Translation: BAG64092.1
CH471054 Genomic DNA Translation: EAW96790.1
BC032226 mRNA Translation: AAH32226.1
M94539 mRNA Translation: AAA58405.1
CCDSiCCDS53800.1 [Q01064-2]
CCDS8882.1 [Q01064-1]
PIRiJC6129
RefSeqiNP_000915.1, NM_000924.3 [Q01064-1]
NP_001159447.1, NM_001165975.2 [Q01064-2]
NP_001275697.1, NM_001288768.1
NP_001275698.1, NM_001288769.1
NP_001302463.1, NM_001315534.1
NP_001302464.1, NM_001315535.1
UniGeneiHs.530871

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LXRmodel-A158-461[»]
1TAZX-ray1.77A146-506[»]
4NPVX-ray2.40A142-507[»]
4NPWX-ray1.90A142-507[»]
5B25X-ray1.90A/B/C/D146-506[»]
5UOYX-ray1.82A146-506[»]
5UP0X-ray2.04A146-506[»]
5W6EX-ray1.90A142-507[»]
ProteinModelPortaliQ01064
SMRiQ01064
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111179, 3 interactors
IntActiQ01064, 2 interactors
MINTiQ01064
STRINGi9606.ENSP00000243052

Chemistry databases

BindingDBiQ01064
ChEMBLiCHEMBL4425
DrugBankiDB01244 Bepridil
DB00201 Caffeine
DB01023 Felodipine
DB00622 Nicardipine
GuidetoPHARMACOLOGYi1295

PTM databases

iPTMnetiQ01064
PhosphoSitePlusiQ01064

Polymorphism and mutation databases

BioMutaiPDE1B
DMDMi3183514

Proteomic databases

MaxQBiQ01064
PaxDbiQ01064
PeptideAtlasiQ01064
PRIDEiQ01064
ProteomicsDBi57904
57905 [Q01064-2]

Protocols and materials databases

DNASUi5153
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000243052; ENSP00000243052; ENSG00000123360 [Q01064-1]
ENST00000550620; ENSP00000448519; ENSG00000123360 [Q01064-2]
GeneIDi5153
KEGGihsa:5153
UCSCiuc001sgd.3 human [Q01064-1]

Organism-specific databases

CTDi5153
DisGeNETi5153
EuPathDBiHostDB:ENSG00000123360.11
GeneCardsiPDE1B
HGNCiHGNC:8775 PDE1B
HPAiHPA018492
MIMi171891 gene
neXtProtiNX_Q01064
OpenTargetsiENSG00000123360
PharmGKBiPA33123
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3688 Eukaryota
ENOG410XQDD LUCA
GeneTreeiENSGT00760000118889
HOGENOMiHOG000231888
HOVERGENiHBG056120
KOiK13755
OMAiTRHTEDN
OrthoDBiEOG091G04C4
PhylomeDBiQ01064
TreeFamiTF314638

Enzyme and pathway databases

BRENDAi3.1.4.17 2681
ReactomeiR-HSA-111957 Cam-PDE 1 activation
R-HSA-418457 cGMP effects
R-HSA-418555 G alpha (s) signalling events
SIGNORiQ01064

Miscellaneous databases

EvolutionaryTraceiQ01064
GeneWikiiPDE1B
GenomeRNAii5153
PROiPR:Q01064
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123360 Expressed in 109 organ(s), highest expression level in caudate nucleus
CleanExiHS_PDE1B
ExpressionAtlasiQ01064 baseline and differential
GenevisibleiQ01064 HS

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR023088 PDEase
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
IPR013706 PDEase_N
PfamiView protein in Pfam
PF00233 PDEase_I, 1 hit
PF08499 PDEase_I_N, 1 hit
PRINTSiPR00387 PDIESTERASE1
SMARTiView protein in SMART
SM00471 HDc, 1 hit
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPDE1B_HUMAN
AccessioniPrimary (citable) accession number: Q01064
Secondary accession number(s): Q92825, Q96KP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1998
Last modified: November 7, 2018
This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
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