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Protein

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B

Gene

PDE1B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate (By similarity).By similarity

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Type I PDE are activated by the binding of calmodulin in the presence of Ca2+. Regulated by phosphorylation by CaM-dependent protein kinase II.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei221Proton donorBy similarity1
Metal bindingi225Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi261Divalent metal cation 1; via tele nitrogenBy similarity1
Metal bindingi262Divalent metal cation 1By similarity1
Metal bindingi262Divalent metal cation 2By similarity1
Metal bindingi368Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
LigandcAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-111957 Cam-PDE 1 activation
R-BTA-418457 cGMP effects
R-BTA-418555 G alpha (s) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B (EC:3.1.4.17)
Short name:
Cam-PDE 1B
Alternative name(s):
63 kDa Cam-PDE
Gene namesi
Name:PDE1B
Synonyms:PDE1B1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Organism-specific databases

VGNCiVGNC:32672 PDE1B

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2650

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987871 – 534Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1BAdd BLAST534

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei464PhosphoserineBy similarity1
Modified residuei512PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ01061
PRIDEiQ01061

Expressioni

Tissue specificityi

Expressed in central nervous system regions. Most abundant in basal ganglia. Also found in kidney papilla and adrenal medulla.

Gene expression databases

BgeeiENSBTAG00000004337
ExpressionAtlasiQ01061 baseline and differential

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi159261, 2 interactors
STRINGi9913.ENSBTAP00000005680

Chemistry databases

BindingDBiQ01061

Structurei

3D structure databases

ProteinModelPortaliQ01061
SMRiQ01061
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini144 – 501PDEasePROSITE-ProRule annotationAdd BLAST358

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 47Calmodulin-bindingSequence analysisAdd BLAST21

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3688 Eukaryota
ENOG410XQDD LUCA
GeneTreeiENSGT00760000118889
HOGENOMiHOG000231888
HOVERGENiHBG056120
InParanoidiQ01061
KOiK13755

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
InterProiView protein in InterPro
IPR003607 HD/PDEase_dom
IPR023088 PDEase
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
IPR013706 PDEase_N
PfamiView protein in Pfam
PF00233 PDEase_I, 1 hit
PF08499 PDEase_I_N, 1 hit
PRINTSiPR00387 PDIESTERASE1
SMARTiView protein in SMART
SM00471 HDc, 1 hit
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q01061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSPRSPPE MLESDCPSPL ELKSAPSKKM WIKLRSLLRY MVKQLENGEV
60 70 80 90 100
NIEELKKNLE YTASLLEAVY IDETRQILDT EDELQELRSD AVPSEVRDWL
110 120 130 140 150
ASTFTQQTRA KGPSEEKPKF RSIVHAVQAG IFVERMFRRT YTSVGPTYST
160 170 180 190 200
AVLNCLKNVD LWCFDVFSLN RAADDHALRT IVFELLTRHN LISRFKIPTV
210 220 230 240 250
FLMTFLDALE TGYGKYKNPY HNQIHAADVT QTVHCFLLRT GMVHCLSEIE
260 270 280 290 300
VLAIIFAAAI HDYEHTGTTN SFHIQTKSEC AILYNDRSVL ENHHISSVFR
310 320 330 340 350
MMQDDEMNIF INLTKDEFVE LRALVIEMVL ATDMSCHFQQ VKSMKTALQQ
360 370 380 390 400
LERIDKSKAL SLLLHAADIS HPTKQWSVHS RWTKALMEEF FRQGDKEAEL
410 420 430 440 450
GLPFSPLCDR TSTLVAQSQI GFIDFIVEPT FSVLTDVAEK SVQPTGDDDS
460 470 480 490 500
KSKNQPSFQW RQPSLDVEVG DPNPDVVSFR STWTKYIQEN KQKWKERAAS
510 520 530
GITNQMSIDE LSPCEEEAPA SPAEDEHNQN GNLD
Length:534
Mass (Da):61,006
Last modified:June 1, 1994 - v1
Checksum:iC86C3F48E0AE9B69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94867 mRNA Translation: AAA74558.1
PIRiA44162
RefSeqiNP_776840.1, NM_174415.2
UniGeneiBt.3910

Genome annotation databases

EnsembliENSBTAT00000003552; ENSBTAP00000003552; ENSBTAG00000004337
GeneIDi281970
KEGGibta:281970

Similar proteinsi

Entry informationi

Entry nameiPDE1B_BOVIN
AccessioniPrimary (citable) accession number: Q01061
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 20, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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