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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

Uchl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).By similarity

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei90NucleophilePROSITE-ProRule annotation1
Active sitei161Proton donorPROSITE-ProRule annotation1
Sitei176Important for enzyme activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.19.12 5301
ReactomeiR-RNO-5689603 UCH proteinases

Protein family/group databases

MEROPSiC12.001

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name:
PGP9.5
Ubiquitin thioesterase L1
Gene namesi
Name:Uchl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi3928 Uchl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002110601 – 220Ubiquitin carboxyl-terminal hydrolase isozyme L1Add BLAST220
PropeptideiPRO_0000414315221 – 223Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei125PhosphoserineCombined sources1
Lipidationi220S-farnesyl cysteineBy similarity1

Post-translational modificationi

O-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ00981
PRIDEiQ00981

2D gel databases

World-2DPAGEi0004:Q00981

PTM databases

iPTMnetiQ00981
PhosphoSitePlusiQ00981
SwissPalmiQ00981

Expressioni

Gene expression databases

BgeeiENSRNOG00000002343 Expressed in 9 organ(s), highest expression level in brain
GenevisibleiQ00981 RN

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 (By similarity). Interacts with SNCA.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248179, 2 interactors
IntActiQ00981, 2 interactors
STRINGi10116.ENSRNOP00000003248

Structurei

3D structure databases

ProteinModelPortaliQ00981
SMRiQ00981
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 10Interaction with ubiquitinBy similarity6
Regioni211 – 216Interaction with ubiquitinBy similarity6

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiKOG1415 Eukaryota
ENOG4111HNA LUCA
GeneTreeiENSGT00510000046640
HOGENOMiHOG000182400
HOVERGENiHBG075483
InParanoidiQ00981
KOiK05611
OMAiFISFVKG
OrthoDBiEOG091G0LBS
PhylomeDBiQ00981

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
IPR030297 UCHL1
PANTHERiPTHR10589 PTHR10589, 1 hit
PTHR10589:SF19 PTHR10589:SF19, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PRINTSiPR00707 UBCTHYDRLASE
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00140 UCH_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q00981-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQLKPMEINP EMLNKVLAKL GVAGQWRFAD VLGLEEETLG SVPSPACALL
60 70 80 90 100
LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA
110 120 130 140 150
NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDSVAQEG
160 170 180 190 200
QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DSLLQDAAKV
210 220
CREFTEREQG EVRFSAVALC KAA
Length:223
Mass (Da):24,838
Last modified:July 5, 2005 - v2
Checksum:iF1402BF7B0C077EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38T → I in BAA01541 (PubMed:1331034).Curated1
Sequence conflicti96I → M in BAA01541 (PubMed:1331034).Curated1
Sequence conflicti105 – 107KLE → NLG in BAA01541 (PubMed:1331034).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10699 mRNA Translation: BAA01541.1
BC060573 mRNA Translation: AAH60573.1
PIRiJX0222
RefSeqiNP_058933.2, NM_017237.3
UniGeneiRn.107213

Genome annotation databases

EnsembliENSRNOT00000003248; ENSRNOP00000003248; ENSRNOG00000002343
GeneIDi29545
KEGGirno:29545
UCSCiRGD:3928 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10699 mRNA Translation: BAA01541.1
BC060573 mRNA Translation: AAH60573.1
PIRiJX0222
RefSeqiNP_058933.2, NM_017237.3
UniGeneiRn.107213

3D structure databases

ProteinModelPortaliQ00981
SMRiQ00981
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248179, 2 interactors
IntActiQ00981, 2 interactors
STRINGi10116.ENSRNOP00000003248

Protein family/group databases

MEROPSiC12.001

PTM databases

iPTMnetiQ00981
PhosphoSitePlusiQ00981
SwissPalmiQ00981

2D gel databases

World-2DPAGEi0004:Q00981

Proteomic databases

PaxDbiQ00981
PRIDEiQ00981

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003248; ENSRNOP00000003248; ENSRNOG00000002343
GeneIDi29545
KEGGirno:29545
UCSCiRGD:3928 rat

Organism-specific databases

CTDi7345
RGDi3928 Uchl1

Phylogenomic databases

eggNOGiKOG1415 Eukaryota
ENOG4111HNA LUCA
GeneTreeiENSGT00510000046640
HOGENOMiHOG000182400
HOVERGENiHBG075483
InParanoidiQ00981
KOiK05611
OMAiFISFVKG
OrthoDBiEOG091G0LBS
PhylomeDBiQ00981

Enzyme and pathway databases

BRENDAi3.4.19.12 5301
ReactomeiR-RNO-5689603 UCH proteinases

Miscellaneous databases

PROiPR:Q00981

Gene expression databases

BgeeiENSRNOG00000002343 Expressed in 9 organ(s), highest expression level in brain
GenevisibleiQ00981 RN

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
IPR030297 UCHL1
PANTHERiPTHR10589 PTHR10589, 1 hit
PTHR10589:SF19 PTHR10589:SF19, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PRINTSiPR00707 UBCTHYDRLASE
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS00140 UCH_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUCHL1_RAT
AccessioniPrimary (citable) accession number: Q00981
Secondary accession number(s): Q6P9V8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 5, 2005
Last modified: November 7, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
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Main funding by: National Institutes of Health

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