UniProtKB - Q00977 (CXB2_MOUSE)
Protein
Gap junction beta-2 protein
Gene
Gjb2
Organism
Mus musculus (Mouse)
Status
Functioni
Structural component of gap junctions (PubMed:15692151). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed:15692151).By similarity1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 42 | Calcium; shared with neighboring subunitBy similarity | 1 | |
Metal bindingi | 45 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 47 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- gap junction channel activity Source: UniProtKB
- gap junction channel activity involved in cell communication by electrical coupling Source: MGI
- identical protein binding Source: MGI
GO - Biological processi
- aging Source: Ensembl
- cell-cell signaling Source: UniProtKB
- cellular response to dexamethasone stimulus Source: Ensembl
- cellular response to glucagon stimulus Source: Ensembl
- cellular response to oxidative stress Source: Ensembl
- decidualization Source: Ensembl
- epididymis development Source: Ensembl
- gap junction assembly Source: MGI
- gap junction-mediated intercellular transport Source: UniProtKB
- inner ear development Source: Ensembl
- response to antibiotic Source: Ensembl
- response to estradiol Source: Ensembl
- response to human chorionic gonadotropin Source: Ensembl
- response to ischemia Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to progesterone Source: Ensembl
- response to retinoic acid Source: Ensembl
- sensory perception of sound Source: UniProtKB-KW
- transmembrane transport Source: MGI
Keywordsi
Biological process | Hearing |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-190872, Transport of connexons to the plasma membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Gap junction beta-2 proteinAlternative name(s): Connexin-26 Short name: Cx26 |
Gene namesi | Name:Gjb2 Synonyms:Cxn-26 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95720, Gjb2 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein By similarity
Other locations
- gap junction 2 Publications
Note: Colocalizes with GJB4 at gap junction plaques in the cochlea.1 Publication
Cytosol
- cytosol Source: MGI
Plasma Membrane
- connexin complex Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- lateral plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- astrocyte projection Source: MGI
- cell body Source: MGI
- cell junction Source: MGI
- cytoplasm Source: MGI
- gap junction Source: MGI
- perinuclear region of cytoplasm Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Intramembranei | 2 – 13 | By similarityAdd BLAST | 12 | |
Topological domaini | 14 – 20 | CytoplasmicCurated | 7 | |
Transmembranei | 21 – 40 | HelicalBy similarityAdd BLAST | 20 | |
Topological domaini | 41 – 73 | ExtracellularCuratedAdd BLAST | 33 | |
Transmembranei | 74 – 94 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 95 – 135 | CytoplasmicCuratedAdd BLAST | 41 | |
Transmembranei | 136 – 156 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 157 – 189 | ExtracellularCuratedAdd BLAST | 33 | |
Transmembranei | 190 – 210 | HelicalBy similarityAdd BLAST | 21 | |
Topological domaini | 211 – 226 | CytoplasmicCuratedAdd BLAST | 16 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Gap junction, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057858 | 1 – 226 | Gap junction beta-2 proteinAdd BLAST | 226 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 53 ↔ 180 | By similarity | ||
Disulfide bondi | 60 ↔ 174 | By similarity | ||
Disulfide bondi | 64 ↔ 169 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | Q00977 |
PaxDbi | Q00977 |
PeptideAtlasi | Q00977 |
PRIDEi | Q00977 |
PTM databases
iPTMneti | Q00977 |
PhosphoSitePlusi | Q00977 |
Expressioni
Tissue specificityi
Detected in cochlea (at protein level) (PubMed:15692151). Detected in cochlea (PubMed:15692151). Liver, kidney, intestine, lung, spleen, stomach, testis and brain, but not heart and adult skeletal muscle.1 Publication
Developmental stagei
Detected in cochlea after 14.5 dpc. Detected in the spiral limbus in neoneates at 2, 8 and 12 days after birth, before the onset of hearing.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000046352, Expressed in cochlea and 256 other tissues |
ExpressionAtlasi | Q00977, baseline and differential |
Genevisiblei | Q00977, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199932, 1 interactor |
IntActi | Q00977, 1 interactor |
STRINGi | 10090.ENSMUSP00000054343 |
Miscellaneous databases
RNActi | Q00977, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QWM8, Eukaryota |
GeneTreei | ENSGT00990000203622 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | Q00977 |
OMAi | YAFYFMY |
OrthoDBi | 1043502at2759 |
PhylomeDBi | Q00977 |
TreeFami | TF329606 |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002268, Connexin26 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01139, CONNEXINB2 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q00977-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDWGTLQSIL GGVNKHSTSI GKIWLTVLFI FRIMILVVAA KEVWGDEQAD
60 70 80 90 100
FVCNTLQPGC KNVCYDHHFP ISHIRLWALQ LIMVSTPALL VAMHVAYRRH
110 120 130 140 150
EKKRKFMKGE IKNEFKDIEE IKTQKVRIEG SLWWTYTTSI FFRVIFEAVF
160 170 180 190 200
MYVFYIMYNG FFMQRLVKCN AWPCPNTVDC FISRPTEKTV FTVFMISVSG
210 220
ICILLNITEL CYLFVRYCSG KSKRPV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81445 Genomic DNA Translation: AAA37495.1 M63803 mRNA Translation: AAA37276.1 BC013634 mRNA Translation: AAH13634.1 BC051437 mRNA Translation: AAH51437.1 |
CCDSi | CCDS27153.1 |
RefSeqi | NP_032151.1, NM_008125.3 |
Genome annotation databases
Ensembli | ENSMUST00000055698; ENSMUSP00000054343; ENSMUSG00000046352 |
GeneIDi | 14619 |
KEGGi | mmu:14619 |
UCSCi | uc007ucx.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M81445 Genomic DNA Translation: AAA37495.1 M63803 mRNA Translation: AAA37276.1 BC013634 mRNA Translation: AAH13634.1 BC051437 mRNA Translation: AAH51437.1 |
CCDSi | CCDS27153.1 |
RefSeqi | NP_032151.1, NM_008125.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 199932, 1 interactor |
IntActi | Q00977, 1 interactor |
STRINGi | 10090.ENSMUSP00000054343 |
PTM databases
iPTMneti | Q00977 |
PhosphoSitePlusi | Q00977 |
Proteomic databases
jPOSTi | Q00977 |
PaxDbi | Q00977 |
PeptideAtlasi | Q00977 |
PRIDEi | Q00977 |
Protocols and materials databases
Antibodypediai | 4587, 319 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000055698; ENSMUSP00000054343; ENSMUSG00000046352 |
GeneIDi | 14619 |
KEGGi | mmu:14619 |
UCSCi | uc007ucx.2, mouse |
Organism-specific databases
CTDi | 2706 |
MGIi | MGI:95720, Gjb2 |
Phylogenomic databases
eggNOGi | ENOG502QWM8, Eukaryota |
GeneTreei | ENSGT00990000203622 |
HOGENOMi | CLU_037388_4_1_1 |
InParanoidi | Q00977 |
OMAi | YAFYFMY |
OrthoDBi | 1043502at2759 |
PhylomeDBi | Q00977 |
TreeFami | TF329606 |
Enzyme and pathway databases
Reactomei | R-MMU-190872, Transport of connexons to the plasma membrane |
Miscellaneous databases
BioGRID-ORCSi | 14619, 0 hits in 17 CRISPR screens |
ChiTaRSi | Gjb2, mouse |
PROi | PR:Q00977 |
RNActi | Q00977, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000046352, Expressed in cochlea and 256 other tissues |
ExpressionAtlasi | Q00977, baseline and differential |
Genevisiblei | Q00977, MM |
Family and domain databases
Gene3Di | 1.20.1440.80, 1 hit |
InterProi | View protein in InterPro IPR000500, Connexin IPR002268, Connexin26 IPR019570, Connexin_CCC IPR017990, Connexin_CS IPR013092, Connexin_N IPR038359, Connexin_N_sf |
PANTHERi | PTHR11984, PTHR11984, 1 hit |
Pfami | View protein in Pfam PF00029, Connexin, 1 hit |
PRINTSi | PR00206, CONNEXIN PR01139, CONNEXINB2 |
SMARTi | View protein in SMART SM00037, CNX, 1 hit SM01089, Connexin_CCC, 1 hit |
PROSITEi | View protein in PROSITE PS00407, CONNEXINS_1, 1 hit PS00408, CONNEXINS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CXB2_MOUSE | |
Accessioni | Q00977Primary (citable) accession number: Q00977 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families