Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Focal adhesion kinase 1

Gene

PTK2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development, embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), ephrin receptors, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Regulates P53/TP53 activity and stability. Phosphorylates SRC; this increases SRC kinase activity. Isoform 2 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling.6 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Activity regulationi

Subject to autoinhibition, mediated by interactions between the FERM domain and the kinase domain. Activated by autophosphorylation at Tyr-397. This promotes interaction with SRC and phosphorylation at Tyr-576 and Tyr-577 in the kinase activation loop. Phosphorylation at Tyr-576 and Tyr-577 is required for maximal kinase activity. Inhibited by TAE226.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei454ATPCurated1
Active sitei546Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi428 – 434ATPCurated7
Nucleotide bindingi500 – 502ATPCurated3

GO - Molecular functioni

  • actin binding Source: Ensembl
  • ATP binding Source: UniProtKB-KW
  • calcium-dependent cysteine-type endopeptidase activity Source: AgBase
  • identical protein binding Source: IntAct
  • integrin binding Source: AgBase
  • JUN kinase binding Source: Ensembl
  • non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  • protease binding Source: AgBase
  • protein tyrosine kinase activity Source: AgBase
  • SH2 domain binding Source: Ensembl
  • signaling receptor binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase, Tyrosine-protein kinase
Biological processAngiogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 1306
ReactomeiR-GGA-111465 Apoptotic cleavage of cellular proteins
R-GGA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-GGA-354192 Integrin alphaIIb beta3 signaling
R-GGA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-GGA-372708 p130Cas linkage to MAPK signaling for integrins
R-GGA-375165 NCAM signaling for neurite out-growth
R-GGA-3928662 EPHB-mediated forward signaling
R-GGA-418885 DCC mediated attractive signaling
R-GGA-4420097 VEGFA-VEGFR2 Pathway
R-GGA-5663213 RHO GTPases Activate WASPs and WAVEs
R-GGA-5673001 RAF/MAP kinase cascade
R-GGA-8874081 MET activates PTK2 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Focal adhesion kinase 1 (EC:2.7.10.2)
Short name:
FADK 1
Alternative name(s):
Focal adhesion kinase-related nonkinase
Short name:
FRNK
Short name:
p41/p43FRNK
Protein-tyrosine kinase 2
p125FAK
pp125FAK
Gene namesi
Name:PTK2
Synonyms:FAK, FAK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi395D → A: Abolishes interaction with PIK3R1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880761 – 1053Focal adhesion kinase 1Add BLAST1053

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei397Phosphotyrosine; by autocatalysis1 Publication1
Modified residuei407PhosphotyrosineBy similarity1
Modified residuei576Phosphotyrosine; by SRC2 Publications1
Modified residuei577Phosphotyrosine; by SRCBy similarity1
Modified residuei863Phosphotyrosine1 Publication1
Modified residuei911Phosphoserine1 Publication1
Modified residuei926PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. Isoform 2 is phosphorylated on serine or threonine residues, but apparently not on tyrosine residues.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ00944
PRIDEiQ00944

PTM databases

iPTMnetiQ00944

Expressioni

Gene expression databases

BgeeiENSGALG00000016171 Expressed in 10 organ(s), highest expression level in colon

Interactioni

Subunit structurei

Interacts with ARHGAP26, GRB7, DCC, PIK3R1, PXN and SRC. Interacts with the ARP2/3 complex.8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi676665, 2 interactors
ELMiQ00944
IntActiQ00944, 11 interactors
STRINGi9031.ENSGALP00000026014

Structurei

Secondary structure

11053
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ00944
SMRiQ00944
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00944

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 355FERMPROSITE-ProRule annotationAdd BLAST321
Domaini422 – 680Protein kinasePROSITE-ProRule annotationAdd BLAST259

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi712 – 733Pro-richAdd BLAST22
Compositional biasi865 – 914Pro-richAdd BLAST50

Domaini

The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4257 Eukaryota
ENOG410ZH9Y LUCA
GeneTreeiENSGT00760000118799
HOGENOMiHOG000069938
HOVERGENiHBG004018
InParanoidiQ00944
KOiK05725
OMAiVQTNHYQ
OrthoDBiEOG091G03BN
PhylomeDBiQ00944

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR000299 FERM_domain
IPR036137 Focal_adhe_kin_target_dom_sf
IPR005189 Focal_adhesion_kin_target_dom
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00373 FERM_M, 1 hit
PF03623 Focal_AT, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006413 Focal_adhesion_target_reg, 1 hit
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
SSF68993 SSF68993, 1 hit
PROSITEiView protein in PROSITE
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q00944-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAYLDPNL NHTPSSSAKT HLGTGMERSP GAMERVLKVF HYFENSSEPT
60 70 80 90 100
TWASIIRHGD ATDVRGIIQK IVDCHKVKNV ACYGLRLSHL QSEEVHWLHL
110 120 130 140 150
DMGVSNVREK FELAHPPEEW KYELRIRYLP KGFLNQFTED KPTLNFFYQQ
160 170 180 190 200
VKNDYMLEIA DQVDQEIALK LGCLEIRRSY GEMRGNALEK KSNYEVLEKD
210 220 230 240 250
VGLRRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI LKFFEILSPV
260 270 280 290 300
YRFDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGANP THLADFNQVQ
310 320 330 340 350
TIQYSNSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL
360 370 380 390 400
VNGATQSFII RPQKEGERAL PSIPKLANNE KQGVRSHTVS VSETDDYAEI
410 420 430 440 450
IDEEDTYTMP STRDYEIQRE RIELGRCIGE GQFGDVHQGI YMSPENPAMA
460 470 480 490 500
VAIKTCKNCT SDSVREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME
510 520 530 540 550
LCTLGELRSF LQVRKFSLDL ASLILYAYQL STALAYLESK RFVHRDIAAR
560 570 580 590 600
NVLVSATDCV KLGDFGLSRY MEDSTYYKAS KGKLPIKWMA PESINFRRFT
610 620 630 640 650
SASDVWMFGV CMWEILMHGV KPFQGVKNND VIGRIENGER LPMPPNCPPT
660 670 680 690 700
LYSLMTKCWA YDPSRRPRFT ELKAQLSTIL EEEKLQQEER MRMESRRQVT
710 720 730 740 750
VSWDSGGSDE APPKPSRPGY PSPRSSEGFY PSPQHMVQPN HYQVSGYSGS
760 770 780 790 800
HGIPAMAGSI YPGQASLLDQ TDSWNHRPQE VSAWQPNMED SGTLDVRGMG
810 820 830 840 850
QVLPTHLMEE RLIRQQQEME EDQRWLEKEE RFLVMKPDVR LSRGSIERED
860 870 880 890 900
GGLQGPAGNQ HIYQPVGKPD HAAPPKKPPR PGAPHLGSLA SLNSPVDSYN
910 920 930 940 950
EGVKIKPQEI SPPPTANLDR SNDKVYENVT GLVKAVIEMS SKIQPAPPEE
960 970 980 990 1000
YVPMVKEVGL ALRTLLATVD ESLPVLPAST HREIEMAQKL LNSDLAELIN
1010 1020 1030 1040 1050
KMKLAQQYVM TSLQQEYKKQ MLTAAHALAV DAKNLLDVID QARLKMISQS

RPH
Length:1,053
Mass (Da):119,207
Last modified:February 1, 1994 - v2
Checksum:i8051154219B953B9
GO
Isoform 2 (identifier: Q00944-2) [UniParc]FASTAAdd to basket
Also known as: FRNK

The sequence of this isoform differs from the canonical sequence as follows:
     1-692: Missing.

Show »
Length:361
Mass (Da):40,032
Checksum:i61E6AD4B50CCCC49
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1D5NY34A0A1D5NY34_CHICK
Focal adhesion kinase 1
PTK2
1,053Annotation score:
A0A1L1RJ83A0A1L1RJ83_CHICK
Focal adhesion kinase 1
PTK2
359Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0421731 – 692Missing in isoform 2. CuratedAdd BLAST692

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86656 mRNA Translation: AAA48765.1
L08402 mRNA Translation: AAA48766.1
PIRiA45388
PC1239
RefSeqiNP_990766.1, NM_205435.1 [Q00944-1]
UniGeneiGga.15614

Genome annotation databases

EnsembliENSGALT00000072414; ENSGALP00000044406; ENSGALG00000031741 [Q00944-1]
GeneIDi396416
KEGGigga:396416

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86656 mRNA Translation: AAA48765.1
L08402 mRNA Translation: AAA48766.1
PIRiA45388
PC1239
RefSeqiNP_990766.1, NM_205435.1 [Q00944-1]
UniGeneiGga.15614

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KTMNMR-A916-1053[»]
1PV3NMR-A920-1053[»]
1QVXNMR-A920-1053[»]
2AEHX-ray2.53A/B31-399[»]
2AL6X-ray2.35A/B31-405[»]
2J0JX-ray2.80A31-686[»]
2J0KX-ray3.00A/B31-686[»]
2J0LX-ray2.30A411-686[»]
2J0MX-ray2.80A31-399[»]
B411-686[»]
2JKKX-ray2.00A411-686[»]
2JKMX-ray2.31A411-686[»]
2JKOX-ray1.65A411-686[»]
2JKQX-ray2.60A411-686[»]
2L6FNMR-A916-1053[»]
2L6GNMR-A916-1053[»]
2L6HNMR-A916-1053[»]
3ZDTX-ray3.15A/B31-405[»]
4BRXX-ray2.05A411-686[»]
4C7TX-ray2.05A411-686[»]
4CYEX-ray3.20A/B31-405[»]
4D4RX-ray1.55A/B411-686[»]
4D4SX-ray2.00A/B411-686[»]
4D4VX-ray2.10A/B411-686[»]
4D4YX-ray1.80A/B411-686[»]
4D55X-ray2.30A411-686[»]
4D58X-ray1.95A/B411-686[»]
4D5HX-ray1.75A/B411-686[»]
4D5KX-ray1.75A/B411-686[»]
ProteinModelPortaliQ00944
SMRiQ00944
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676665, 2 interactors
ELMiQ00944
IntActiQ00944, 11 interactors
STRINGi9031.ENSGALP00000026014

PTM databases

iPTMnetiQ00944

Proteomic databases

PaxDbiQ00944
PRIDEiQ00944

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000072414; ENSGALP00000044406; ENSGALG00000031741 [Q00944-1]
GeneIDi396416
KEGGigga:396416

Organism-specific databases

CTDi5747

Phylogenomic databases

eggNOGiKOG4257 Eukaryota
ENOG410ZH9Y LUCA
GeneTreeiENSGT00760000118799
HOGENOMiHOG000069938
HOVERGENiHBG004018
InParanoidiQ00944
KOiK05725
OMAiVQTNHYQ
OrthoDBiEOG091G03BN
PhylomeDBiQ00944

Enzyme and pathway databases

BRENDAi2.7.10.2 1306
ReactomeiR-GGA-111465 Apoptotic cleavage of cellular proteins
R-GGA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-GGA-354192 Integrin alphaIIb beta3 signaling
R-GGA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-GGA-372708 p130Cas linkage to MAPK signaling for integrins
R-GGA-375165 NCAM signaling for neurite out-growth
R-GGA-3928662 EPHB-mediated forward signaling
R-GGA-418885 DCC mediated attractive signaling
R-GGA-4420097 VEGFA-VEGFR2 Pathway
R-GGA-5663213 RHO GTPases Activate WASPs and WAVEs
R-GGA-5673001 RAF/MAP kinase cascade
R-GGA-8874081 MET activates PTK2 signaling

Miscellaneous databases

EvolutionaryTraceiQ00944
PROiPR:Q00944

Gene expression databases

BgeeiENSGALG00000016171 Expressed in 10 organ(s), highest expression level in colon

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR000299 FERM_domain
IPR036137 Focal_adhe_kin_target_dom_sf
IPR005189 Focal_adhesion_kin_target_dom
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF00373 FERM_M, 1 hit
PF03623 Focal_AT, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00109 TYRKINASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006413 Focal_adhesion_target_reg, 1 hit
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
SSF56112 SSF56112, 1 hit
SSF68993 SSF68993, 1 hit
PROSITEiView protein in PROSITE
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFAK1_CHICK
AccessioniPrimary (citable) accession number: Q00944
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1994
Last modified: September 12, 2018
This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again