PTK2

Functionⁱ

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development, embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), ephrin receptors, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Regulates P53/TP53 activity and stability. Phosphorylates SRC; this increases SRC kinase activity. Isoform 2 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling.6 Publications

Catalytic activityⁱ

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Activity regulationⁱ

Subject to autoinhibition, mediated by interactions between the FERM domain and the kinase domain. Activated by autophosphorylation at Tyr-397. This promotes interaction with SRC and phosphorylation at Tyr-576 and Tyr-577 in the kinase activation loop. Phosphorylation at Tyr-576 and Tyr-577 is required for maximal kinase activity. Inhibited by TAE226.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	454	ATPCurated		1
Active siteⁱ	546	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	428 – 434	ATPCurated		7
Nucleotide bindingⁱ	500 – 502	ATPCurated		3

GO - Molecular functionⁱ

actin binding Source: Ensembl
ATP binding Source: UniProtKB-KW
calcium-dependent cysteine-type endopeptidase activity
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 17721515
- 20802513
integrin binding
Source: AgBase
Inferred from physical interactionⁱ
- 17604018
JUN kinase binding Source: Ensembl
non-membrane spanning protein tyrosine kinase activity Source: GO_Central
protease binding
Source: AgBase
Inferred from physical interactionⁱ
- 15340073
protein tyrosine kinase activity
Source: AgBase
Inferred from direct assayⁱ
- 1594631
SH2 domain binding Source: Ensembl
signaling receptor binding Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin filament organization
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
angiogenesis involved in wound healing
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15537495
central nervous system neuron axonogenesis Source: Ensembl
endothelial cell migration Source: Ensembl
ephrin receptor signaling pathway Source: Ensembl
epidermal growth factor receptor signaling pathway Source: GO_Central
extracellular matrix organization Source: Ensembl
growth hormone receptor signaling pathway Source: Ensembl
innate immune response Source: GO_Central
integrin-mediated signaling pathway Source: Ensembl
microtubule cytoskeleton organization Source: Ensembl
negative regulation of anoikis
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
negative regulation of autophagy Source: Ensembl
negative regulation of axonogenesis Source: Ensembl
negative regulation of cell-cell adhesion Source: Ensembl
negative regulation of cell-substrate adhesion
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
negative regulation of organ growth Source: Ensembl
negative regulation of protein autophosphorylation
Source: AgBase
Inferred from mutant phenotypeⁱ
- 23393139
negative regulation of synapse assembly Source: Ensembl
nuclear migration Source: Ensembl
peptidyl-tyrosine autophosphorylation Source: GO_Central
positive regulation of cardiac muscle hypertrophy Source: Ensembl
positive regulation of cell proliferation
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
positive regulation of focal adhesion assembly
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
positive regulation of protein binding
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
positive regulation of protein kinase B signaling Source: Ensembl
positive regulation of protein tyrosine kinase activity
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
positive regulation of substrate-dependent cell migration, cell attachment to substrate
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
positive regulation of ubiquitin-dependent protein catabolic process Source: Ensembl
protein autophosphorylation
Source: AgBase
Inferred from direct assayⁱ
- 10409118
pyramidal neuron migration
Source: AgBase
Inferred from mutant phenotypeⁱ
- 21832197
regulation of cell adhesion mediated by integrin Source: Ensembl
regulation of cell shape Source: Ensembl
regulation of epithelial cell migration Source: Ensembl
regulation of osteoblast differentiation Source: Ensembl
regulation of substrate adhesion-dependent cell spreading Source: Ensembl
response to muscle stretch
Source: AgBase
Inferred from direct assayⁱ
- 10409118
response to pH
Source: AgBase
Inferred from mutant phenotypeⁱ
- 23393139
signal complex assembly Source: InterPro
transforming growth factor beta receptor signaling pathway Source: Ensembl
vasculogenesis Source: Ensembl
wound healing, spreading of cells
Source: AgBase
Inferred from mutant phenotypeⁱ
- 15340073
- 23393139

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Developmental protein, Kinase, Transferase, Tyrosine-protein kinase
Biological process	Angiogenesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.10.2 1306
Reactomeⁱ	R-GGA-111465 Apoptotic cleavage of cellular proteins R-GGA-2029482 Regulation of actin dynamics for phagocytic cup formation R-GGA-354192 Integrin alphaIIb beta3 signaling R-GGA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins R-GGA-372708 p130Cas linkage to MAPK signaling for integrins R-GGA-375165 NCAM signaling for neurite out-growth R-GGA-3928662 EPHB-mediated forward signaling R-GGA-418885 DCC mediated attractive signaling R-GGA-4420097 VEGFA-VEGFR2 Pathway R-GGA-5663213 RHO GTPases Activate WASPs and WAVEs R-GGA-5673001 RAF/MAP kinase cascade R-GGA-8874081 MET activates PTK2 signaling

BRENDAⁱ

2.7.10.2 1306

Reactomeⁱ

R-GGA-111465 Apoptotic cleavage of cellular proteins
R-GGA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-GGA-354192 Integrin alphaIIb beta3 signaling
R-GGA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-GGA-372708 p130Cas linkage to MAPK signaling for integrins
R-GGA-375165 NCAM signaling for neurite out-growth
R-GGA-3928662 EPHB-mediated forward signaling
R-GGA-418885 DCC mediated attractive signaling
R-GGA-4420097 VEGFA-VEGFR2 Pathway
R-GGA-5663213 RHO GTPases Activate WASPs and WAVEs
R-GGA-5673001 RAF/MAP kinase cascade
R-GGA-8874081 MET activates PTK2 signaling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Focal adhesion kinase 1 (EC:2.7.10.2) Short name: FADK 1 Alternative name(s): Focal adhesion kinase-related nonkinase Short name: FRNK Short name: p41/p43FRNK Protein-tyrosine kinase 2 p125FAK pp125FAK
Gene namesⁱ	Name:PTK2 Synonyms:FAK, FAK1
Organismⁱ	Gallus gallus (Chicken)
Taxonomic identifierⁱ	9031 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Sauropsida › Sauria › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus
Proteomesⁱ	UP000000539 Componentⁱ: Chromosome 2

Keywords - Cellular componentⁱ

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	395	D → A: Abolishes interaction with PIK3R1. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000088076	1 – 1053	Focal adhesion kinase 1Add BLAST		1053

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	397	Phosphotyrosine; by autocatalysis1 Publication	1
Modified residueⁱ	407	PhosphotyrosineBy similarity	1
Modified residueⁱ	576	Phosphotyrosine; by SRC2 Publications	1
Modified residueⁱ	577	Phosphotyrosine; by SRCBy similarity	1
Modified residueⁱ	863	Phosphotyrosine1 Publication	1
Modified residueⁱ	911	Phosphoserine1 Publication	1
Modified residueⁱ	926	PhosphotyrosineBy similarity	1

Post-translational modificationⁱ

Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. Isoform 2 is phosphorylated on serine or threonine residues, but apparently not on tyrosine residues.4 Publications

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q00944
PRIDEⁱ	Q00944

PTM databases

iPTMnetⁱ	Q00944

iPTMnetⁱ

Q00944

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSGALG00000016171 Expressed in 10 organ(s), highest expression level in colon

Bgeeⁱ

ENSGALG00000016171 Expressed in 10 organ(s), highest expression level in colon

Interactionⁱ

Subunit structureⁱ

Interacts with ARHGAP26, GRB7, DCC, PIK3R1, PXN and SRC. Interacts with the ARP2/3 complex.8 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-2896409,EBI-2896409
ACTR3	P61157	5	EBI-2896409,EBI-351419	From Bos taurus.
MET	P08581	5	EBI-2896409,EBI-1039152	From Homo sapiens.
PXN	P49024	2	EBI-2896409,EBI-2896280
SRC	P00523	3	EBI-2896409,EBI-848039

GO - Molecular functionⁱ

actin binding Source: Ensembl
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 17721515
- 20802513
integrin binding
Source: AgBase
Inferred from physical interactionⁱ
- 17604018
JUN kinase binding Source: Ensembl
protease binding
Source: AgBase
Inferred from physical interactionⁱ
- 15340073
SH2 domain binding Source: Ensembl
signaling receptor binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	676665, 2 interactors
ELMⁱ	Q00944
IntActⁱ	Q00944, 11 interactors
STRINGⁱ	9031.ENSGALP00000026014

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	36 – 40	Combined sources	5
Helixⁱ	49 – 51	Combined sources	3
Beta strandⁱ	53 – 58	Combined sources	6
Helixⁱ	64 – 74	Combined sources	11
Helixⁱ	80 – 82	Combined sources	3
Beta strandⁱ	83 – 89	Combined sources	7
Beta strandⁱ	95 – 98	Combined sources	4
Beta strandⁱ	100 – 103	Combined sources	4
Helixⁱ	104 – 112	Combined sources	9
Helixⁱ	117 – 119	Combined sources	3
Beta strandⁱ	120 – 126	Combined sources	7
Helixⁱ	133 – 137	Combined sources	5
Helixⁱ	141 – 158	Combined sources	18
Turnⁱ	159 – 162	Combined sources	4
Helixⁱ	165 – 179	Combined sources	15
Turnⁱ	180 – 182	Combined sources	3
Helixⁱ	187 – 189	Combined sources	3
Helixⁱ	191 – 199	Combined sources	9
Turnⁱ	200 – 202	Combined sources	3
Helixⁱ	203 – 205	Combined sources	3
Helixⁱ	209 – 213	Combined sources	5
Helixⁱ	217 – 229	Combined sources	13
Turnⁱ	230 – 233	Combined sources	4
Helixⁱ	236 – 247	Combined sources	12
Turnⁱ	248 – 250	Combined sources	3
Beta strandⁱ	256 – 262	Combined sources	7
Beta strandⁱ	264 – 266	Combined sources	3
Beta strandⁱ	268 – 275	Combined sources	8
Turnⁱ	276 – 278	Combined sources	3
Beta strandⁱ	279 – 285	Combined sources	7
Beta strandⁱ	290 – 294	Combined sources	5
Helixⁱ	296 – 298	Combined sources	3
Beta strandⁱ	299 – 305	Combined sources	7
Beta strandⁱ	308 – 310	Combined sources	3
Beta strandⁱ	314 – 320	Combined sources	7
Beta strandⁱ	327 – 333	Combined sources	7
Helixⁱ	334 – 352	Combined sources	19
Beta strandⁱ	398 – 402	Combined sources	5
Helixⁱ	412 – 414	Combined sources	3
Helixⁱ	419 – 421	Combined sources	3
Beta strandⁱ	422 – 431	Combined sources	10
Beta strandⁱ	434 – 441	Combined sources	8
Beta strandⁱ	444 – 446	Combined sources	3
Beta strandⁱ	449 – 455	Combined sources	7
Turnⁱ	457 – 460	Combined sources	4
Helixⁱ	462 – 475	Combined sources	14
Beta strandⁱ	486 – 490	Combined sources	5
Beta strandⁱ	492 – 494	Combined sources	3
Beta strandⁱ	496 – 500	Combined sources	5
Helixⁱ	507 – 514	Combined sources	8
Turnⁱ	515 – 517	Combined sources	3
Helixⁱ	520 – 539	Combined sources	20
Helixⁱ	549 – 551	Combined sources	3
Beta strandⁱ	552 – 556	Combined sources	5
Beta strandⁱ	559 – 562	Combined sources	4
Helixⁱ	567 – 570	Combined sources	4
Helixⁱ	572 – 575	Combined sources	4
Helixⁱ	577 – 579	Combined sources	3
Helixⁱ	586 – 588	Combined sources	3
Helixⁱ	591 – 596	Combined sources	6
Helixⁱ	601 – 616	Combined sources	16
Turnⁱ	617 – 619	Combined sources	3
Turnⁱ	622 – 625	Combined sources	4
Helixⁱ	628 – 630	Combined sources	3
Helixⁱ	631 – 636	Combined sources	6
Helixⁱ	649 – 658	Combined sources	10
Helixⁱ	663 – 665	Combined sources	3
Helixⁱ	669 – 684	Combined sources	16
Turnⁱ	919 – 921	Combined sources	3
Helixⁱ	922 – 941	Combined sources	20
Turnⁱ	942 – 945	Combined sources	4
Helixⁱ	948 – 975	Combined sources	28
Turnⁱ	978 – 980	Combined sources	3
Helixⁱ	982 – 1005	Combined sources	24
Beta strandⁱ	1008 – 1012	Combined sources	5
Helixⁱ	1014 – 1047	Combined sources	34

Show more details

3D structure databases

ProteinModelPortalⁱ	Q00944
SMRⁱ	Q00944
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q00944

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	35 – 355	FERMPROSITE-ProRule annotationAdd BLAST		321
Domainⁱ	422 – 680	Protein kinasePROSITE-ProRule annotationAdd BLAST		259

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	712 – 733	Pro-richAdd BLAST		22
Compositional biasⁱ	865 – 914	Pro-richAdd BLAST		50

Domainⁱ

The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG4257 Eukaryota ENOG410ZH9Y LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118799
HOGENOMⁱ	HOG000069938
HOVERGENⁱ	HBG004018
InParanoidⁱ	Q00944
KEGG Orthology (KO) More...KOⁱ	K05725
OMAⁱ	VQTNHYQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03BN
PhylomeDBⁱ	Q00944

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd14473 FERM_B-lobe, 1 hit
Gene3Dⁱ	1.20.80.10, 1 hit 2.30.29.30, 1 hit
InterProⁱ	View protein in InterPro IPR019749 Band_41_domain IPR014352 FERM/acyl-CoA-bd_prot_sf IPR035963 FERM_2 IPR019748 FERM_central IPR000299 FERM_domain IPR036137 Focal_adhe_kin_target_dom_sf IPR005189 Focal_adhesion_kin_target_dom IPR011009 Kinase-like_dom_sf IPR011993 PH-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom IPR029071 Ubiquitin-like_domsf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00373 FERM_M, 1 hit PF03623 Focal_AT, 1 hit PF07714 Pkinase_Tyr, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD006413 Focal_adhesion_target_reg, 1 hit
SMARTⁱ	View protein in SMART SM00295 B41, 1 hit SM00219 TyrKc, 1 hit
SUPFAMⁱ	SSF47031 SSF47031, 1 hit SSF54236 SSF54236, 1 hit SSF56112 SSF56112, 1 hit SSF68993 SSF68993, 1 hit
PROSITEⁱ	View protein in PROSITE PS00661 FERM_2, 1 hit PS50057 FERM_3, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative promoter usage. Align Add to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.ⁱShow all

Isoform 1 (identifier: Q00944-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAYLDPNL NHTPSSSAKT HLGTGMERSP GAMERVLKVF HYFENSSEPT 
        60         70         80         90        100
TWASIIRHGD ATDVRGIIQK IVDCHKVKNV ACYGLRLSHL QSEEVHWLHL 
       110        120        130        140        150
DMGVSNVREK FELAHPPEEW KYELRIRYLP KGFLNQFTED KPTLNFFYQQ 
       160        170        180        190        200
VKNDYMLEIA DQVDQEIALK LGCLEIRRSY GEMRGNALEK KSNYEVLEKD 
       210        220        230        240        250
VGLRRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI LKFFEILSPV 
       260        270        280        290        300
YRFDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGANP THLADFNQVQ 
       310        320        330        340        350
TIQYSNSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL 
       360        370        380        390        400
VNGATQSFII RPQKEGERAL PSIPKLANNE KQGVRSHTVS VSETDDYAEI 
       410        420        430        440        450
IDEEDTYTMP STRDYEIQRE RIELGRCIGE GQFGDVHQGI YMSPENPAMA 
       460        470        480        490        500
VAIKTCKNCT SDSVREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME 
       510        520        530        540        550
LCTLGELRSF LQVRKFSLDL ASLILYAYQL STALAYLESK RFVHRDIAAR 
       560        570        580        590        600
NVLVSATDCV KLGDFGLSRY MEDSTYYKAS KGKLPIKWMA PESINFRRFT 
       610        620        630        640        650
SASDVWMFGV CMWEILMHGV KPFQGVKNND VIGRIENGER LPMPPNCPPT 
       660        670        680        690        700
LYSLMTKCWA YDPSRRPRFT ELKAQLSTIL EEEKLQQEER MRMESRRQVT 
       710        720        730        740        750
VSWDSGGSDE APPKPSRPGY PSPRSSEGFY PSPQHMVQPN HYQVSGYSGS 
       760        770        780        790        800
HGIPAMAGSI YPGQASLLDQ TDSWNHRPQE VSAWQPNMED SGTLDVRGMG 
       810        820        830        840        850
QVLPTHLMEE RLIRQQQEME EDQRWLEKEE RFLVMKPDVR LSRGSIERED 
       860        870        880        890        900
GGLQGPAGNQ HIYQPVGKPD HAAPPKKPPR PGAPHLGSLA SLNSPVDSYN 
       910        920        930        940        950
EGVKIKPQEI SPPPTANLDR SNDKVYENVT GLVKAVIEMS SKIQPAPPEE 
       960        970        980        990       1000
YVPMVKEVGL ALRTLLATVD ESLPVLPAST HREIEMAQKL LNSDLAELIN 
      1010       1020       1030       1040       1050
KMKLAQQYVM TSLQQEYKKQ MLTAAHALAV DAKNLLDVID QARLKMISQS 

RPH

Length:1,053

Mass (Da):119,207

Last modified:February 1, 1994 - v2

Checksum:ⁱ8051154219B953B9

GO

Isoform 2 (identifier: Q00944-2) [UniParc]FASTA Add to basket

Also known as: FRNK

The sequence of this isoform differs from the canonical sequence as follows:
1-692: Missing.

« Hide

        10         20         30         40         50
MESRRQVTVS WDSGGSDEAP PKPSRPGYPS PRSSEGFYPS PQHMVQPNHY 
        60         70         80         90        100
QVSGYSGSHG IPAMAGSIYP GQASLLDQTD SWNHRPQEVS AWQPNMEDSG 
       110        120        130        140        150
TLDVRGMGQV LPTHLMEERL IRQQQEMEED QRWLEKEERF LVMKPDVRLS 
       160        170        180        190        200
RGSIEREDGG LQGPAGNQHI YQPVGKPDHA APPKKPPRPG APHLGSLASL 
       210        220        230        240        250
NSPVDSYNEG VKIKPQEISP PPTANLDRSN DKVYENVTGL VKAVIEMSSK 
       260        270        280        290        300
IQPAPPEEYV PMVKEVGLAL RTLLATVDES LPVLPASTHR EIEMAQKLLN 
       310        320        330        340        350
SDLAELINKM KLAQQYVMTS LQQEYKKQML TAAHALAVDA KNLLDVIDQA 
       360 
RLKMISQSRP H

Show »

Length:361

Mass (Da):40,032

Checksum:ⁱ61E6AD4B50CCCC49

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	A0A1D5NY34	A0A1D5NY34_CHICK		Focal adhesion kinase 1 Focal adhesion kinase 1	PTK2	1,053	Annotation score:
	A0A1L1RJ83	A0A1L1RJ83_CHICK		Focal adhesion kinase 1 Focal adhesion kinase 1	PTK2	359	Annotation score:

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_042173	1 – 692	Missing in isoform 2. CuratedAdd BLAST		692

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M86656 mRNA Translation: AAA48765.1 L08402 mRNA Translation: AAA48766.1
PIRⁱ	A45388 PC1239
NCBI Reference Sequences More...RefSeqⁱ	NP_990766.1, NM_205435.1 [Q00944-1]
UniGeneⁱ	Gga.15614

Genome annotation databases

Ensemblⁱ	ENSGALT00000072414; ENSGALP00000044406; ENSGALG00000031741 [Q00944-1]
GeneIDⁱ	396416
KEGGⁱ	gga:396416

Keywords - Coding sequence diversityⁱ

Alternative promoter usage

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q00944	Isoform 2 of Focal adhesion kinase 1		CHICK		361	UniRef100_Q00944
UPI000157348B		CHICK		656
UPI000181CD3C		CHICK		276

Protein	Similar proteins		Species	Score	Length	Source
Q00944	Focal adhesion kinase 1	)	HUMAN		1052	UniRef90_Q05397
Focal adhesion kinase 1		XENLA		1068
Focal adhesion kinase 1		RAT		1055
focal adhesion kinase 1 isoform X3		ERIEU		1112
focal adhesion kinase 1 isoform X4		TARSY		1112
+739

Protein	Similar proteins		Species	Score	Length	Source
Q00944	Focal adhesion kinase 1	)	HUMAN		1052	UniRef50_Q05397
Focal adhesion kinase 1		XENLA		1068
Focal adhesion kinase 1		RAT		1055
Focal adhesion kinase 1	)	MOUSE		1090
focal adhesion kinase 1 isoform X2		Enhydra lutris kenyoni		1112
+1801

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M86656 mRNA Translation: AAA48765.1 L08402 mRNA Translation: AAA48766.1
PIRⁱ	A45388 PC1239
RefSeqⁱ	NP_990766.1, NM_205435.1 [Q00944-1]
UniGeneⁱ	Gga.15614

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1KTM	NMR	-	A	916-1053	[»]
	1PV3	NMR	-	A	920-1053	[»]
	1QVX	NMR	-	A	920-1053	[»]
	2AEH	X-ray	2.53	A/B	31-399	[»]
	2AL6	X-ray	2.35	A/B	31-405	[»]
	2J0J	X-ray	2.80	A	31-686	[»]
	2J0K	X-ray	3.00	A/B	31-686	[»]
	2J0L	X-ray	2.30	A	411-686	[»]
	2J0M	X-ray	2.80	A	31-399	[»]
				B	411-686	[»]
	2JKK	X-ray	2.00	A	411-686	[»]
	2JKM	X-ray	2.31	A	411-686	[»]
	2JKO	X-ray	1.65	A	411-686	[»]
	2JKQ	X-ray	2.60	A	411-686	[»]
	2L6F	NMR	-	A	916-1053	[»]
	2L6G	NMR	-	A	916-1053	[»]
	2L6H	NMR	-	A	916-1053	[»]
	3ZDT	X-ray	3.15	A/B	31-405	[»]
	4BRX	X-ray	2.05	A	411-686	[»]
	4C7T	X-ray	2.05	A	411-686	[»]
	4CYE	X-ray	3.20	A/B	31-405	[»]
	4D4R	X-ray	1.55	A/B	411-686	[»]
	4D4S	X-ray	2.00	A/B	411-686	[»]
	4D4V	X-ray	2.10	A/B	411-686	[»]
	4D4Y	X-ray	1.80	A/B	411-686	[»]
	4D55	X-ray	2.30	A	411-686	[»]
	4D58	X-ray	1.95	A/B	411-686	[»]
	4D5H	X-ray	1.75	A/B	411-686	[»]
	4D5K	X-ray	1.75	A/B	411-686	[»]
ProteinModelPortalⁱ	Q00944
SMRⁱ	Q00944
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	676665, 2 interactors
ELMⁱ	Q00944
IntActⁱ	Q00944, 11 interactors
STRINGⁱ	9031.ENSGALP00000026014

PTM databases

iPTMnetⁱ	Q00944

iPTMnetⁱ

Q00944

Proteomic databases

PaxDbⁱ	Q00944
PRIDEⁱ	Q00944

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSGALT00000072414; ENSGALP00000044406; ENSGALG00000031741 [Q00944-1]
GeneIDⁱ	396416
KEGGⁱ	gga:396416

Organism-specific databases

CTDⁱ	5747

CTDⁱ

5747

Phylogenomic databases

eggNOGⁱ	KOG4257 Eukaryota ENOG410ZH9Y LUCA
GeneTreeⁱ	ENSGT00760000118799
HOGENOMⁱ	HOG000069938
HOVERGENⁱ	HBG004018
InParanoidⁱ	Q00944
KOⁱ	K05725
OMAⁱ	VQTNHYQ
OrthoDBⁱ	EOG091G03BN
PhylomeDBⁱ	Q00944

Enzyme and pathway databases

BRENDAⁱ	2.7.10.2 1306
Reactomeⁱ	R-GGA-111465 Apoptotic cleavage of cellular proteins R-GGA-2029482 Regulation of actin dynamics for phagocytic cup formation R-GGA-354192 Integrin alphaIIb beta3 signaling R-GGA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins R-GGA-372708 p130Cas linkage to MAPK signaling for integrins R-GGA-375165 NCAM signaling for neurite out-growth R-GGA-3928662 EPHB-mediated forward signaling R-GGA-418885 DCC mediated attractive signaling R-GGA-4420097 VEGFA-VEGFR2 Pathway R-GGA-5663213 RHO GTPases Activate WASPs and WAVEs R-GGA-5673001 RAF/MAP kinase cascade R-GGA-8874081 MET activates PTK2 signaling

BRENDAⁱ

2.7.10.2 1306

Reactomeⁱ

R-GGA-111465 Apoptotic cleavage of cellular proteins
R-GGA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-GGA-354192 Integrin alphaIIb beta3 signaling
R-GGA-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-GGA-372708 p130Cas linkage to MAPK signaling for integrins
R-GGA-375165 NCAM signaling for neurite out-growth
R-GGA-3928662 EPHB-mediated forward signaling
R-GGA-418885 DCC mediated attractive signaling
R-GGA-4420097 VEGFA-VEGFR2 Pathway
R-GGA-5663213 RHO GTPases Activate WASPs and WAVEs
R-GGA-5673001 RAF/MAP kinase cascade
R-GGA-8874081 MET activates PTK2 signaling

Miscellaneous databases

EvolutionaryTraceⁱ	Q00944
Protein Ontology More...PROⁱ	PR:Q00944

Gene expression databases

Bgeeⁱ	ENSGALG00000016171 Expressed in 10 organ(s), highest expression level in colon

Bgeeⁱ

ENSGALG00000016171 Expressed in 10 organ(s), highest expression level in colon

Family and domain databases

CDDⁱ	cd14473 FERM_B-lobe, 1 hit
Gene3Dⁱ	1.20.80.10, 1 hit 2.30.29.30, 1 hit
InterProⁱ	View protein in InterPro IPR019749 Band_41_domain IPR014352 FERM/acyl-CoA-bd_prot_sf IPR035963 FERM_2 IPR019748 FERM_central IPR000299 FERM_domain IPR036137 Focal_adhe_kin_target_dom_sf IPR005189 Focal_adhesion_kin_target_dom IPR011009 Kinase-like_dom_sf IPR011993 PH-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom IPR029071 Ubiquitin-like_domsf
Pfamⁱ	View protein in Pfam PF00373 FERM_M, 1 hit PF03623 Focal_AT, 1 hit PF07714 Pkinase_Tyr, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD006413 Focal_adhesion_target_reg, 1 hit
SMARTⁱ	View protein in SMART SM00295 B41, 1 hit SM00219 TyrKc, 1 hit
SUPFAMⁱ	SSF47031 SSF47031, 1 hit SSF54236 SSF54236, 1 hit SSF56112 SSF56112, 1 hit SSF68993 SSF68993, 1 hit
PROSITEⁱ	View protein in PROSITE PS00661 FERM_2, 1 hit PS50057 FERM_3, 1 hit PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FAK1_CHICK
Accessionⁱ	Q00944Primary (citable) accession number: Q00944
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
	Last sequence update:	February 1, 1994
	Last modified:	September 12, 2018
	This is version 177 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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Supporting data

UniProtKB - Q00944 (FAK1_CHICK)

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Focal adhesion kinase 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Plasma membrane

Cytoskeleton

Other locations

Cytoskeleton

Cytosol

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Computationally mapped potential isoform sequencesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ