UniProtKB - Q00764 (TPS1_YEAST)
Protein
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit
Gene
TPS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Synthase catalytic subunit of the trehalose synthase complex that catalyzes the production of trehalose from glucose-6-phosphate and UDP-alpha-D-glucose in a two step process. Can function independently of the complex.1 Publication
Miscellaneous
Present with 10900 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:2.4.1.15
Activity regulationi
Activated by fructose 6-phosphate. Inorganic phosphate inhibits the synthase activity in the complex, but activates the synthase activity in the free monomeric form.1 Publication
Kineticsi
- KM=3.5 mM for D-glucose 6-phosphate1 Publication
- KM=0.5 mM for UDP-alpha-D-glucose1 Publication
GO - Molecular functioni
- alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: SGD
- trehalose-phosphatase activity Source: SGD
GO - Biological processi
- cellular carbohydrate metabolic process Source: SGD
- cellular response to desiccation Source: SGD
- cellular response to heat Source: SGD
- cellular response to osmotic stress Source: SGD
- cellular response to oxidative stress Source: SGD
- trehalose biosynthetic process Source: SGD
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | MetaCyc:YBR126C-MONOMER |
BRENDAi | 2.4.1.15, 984 |
Protein family/group databases
CAZyi | GT20, Glycosyltransferase Family 20 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunit (EC:2.4.1.15)Alternative name(s): General glucose sensor subunit 1 Glycogen metabolism control protein GLC6 Trehalose synthase complex catalytic subunit TPS1 Trehalose-6-phosphate synthase UDP-glucose-glucosephosphate glucosyltransferase |
Gene namesi | Name:TPS1 Synonyms:BYP1, CIF1, FDP1, GGS1, GLC6, TSS1 Ordered Locus Names:YBR126C ORF Names:YBR0922 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YBR126C |
SGDi | S000000330, TPS1 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: GO_Central
Other locations
- alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) Source: SGD
- cytoplasm Source: SGD
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 223 | H → Y in GLC6-1; low glycogen accumulation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122502 | 1 – 495 | Alpha,alpha-trehalose-phosphate synthase [UDP-forming] 56 kDa subunitAdd BLAST | 495 |
Proteomic databases
MaxQBi | Q00764 |
PaxDbi | Q00764 |
PRIDEi | Q00764 |
TopDownProteomicsi | Q00764 |
PTM databases
CarbonylDBi | Q00764 |
iPTMneti | Q00764 |
Expressioni
Inductioni
Either partial repression by glucose or induction by galactose. Induced by heat shock.
Interactioni
Subunit structurei
The trehalose synthase complex is composed of the two catalytic subunits TPS1 and TPS2 and at least one of the two regulatory subunits TPS3 or TSL1.
1 PublicationBinary interactionsi
Hide detailsQ00764
With | #Exp. | IntAct |
---|---|---|
TPS2 [P31688] | 7 | EBI-19430,EBI-19440 |
TPS3 [P38426] | 5 | EBI-19430,EBI-19448 |
TSL1 [P38427] | 6 | EBI-19430,EBI-19638 |
Protein-protein interaction databases
BioGRIDi | 32827, 482 interactors |
ComplexPortali | CPX-582, Trehalose-6-phosphate synthase/phosphatase variant 1 CPX-583, Trehalose-6-phosphate synthase/phosphatase variant 2 |
DIPi | DIP-744N |
IntActi | Q00764, 39 interactors |
MINTi | Q00764 |
STRINGi | 4932.YBR126C |
Miscellaneous databases
RNActi | Q00764, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 20 family.Curated
Phylogenomic databases
eggNOGi | KOG1050, Eukaryota |
GeneTreei | ENSGT00940000167933 |
HOGENOMi | CLU_002351_7_2_1 |
InParanoidi | Q00764 |
OMAi | YIACQQE |
Family and domain databases
InterProi | View protein in InterPro IPR001830, Glyco_trans_20 IPR012766, Trehalose_OtsA |
Pfami | View protein in Pfam PF00982, Glyco_transf_20, 1 hit |
TIGRFAMsi | TIGR02400, trehalose_OtsA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q00764-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTDNAKAQL TSSSGGNIIV VSNRLPVTIT KNSSTGQYEY AMSSGGLVTA
60 70 80 90 100
LEGLKKTYTF KWFGWPGLEI PDDEKDQVRK DLLEKFNAVP IFLSDEIADL
110 120 130 140 150
HYNGFSNSIL WPLFHYHPGE INFDENAWLA YNEANQTFTN EIAKTMNHND
160 170 180 190 200
LIWVHDYHLM LVPEMLRVKI HEKQLQNVKV GWFLHTPFPS SEIYRILPVR
210 220 230 240 250
QEILKGVLSC DLVGFHTYDY ARHFLSSVQR VLNVNTLPNG VEYQGRFVNV
260 270 280 290 300
GAFPIGIDVD KFTDGLKKES VQKRIQQLKE TFKGCKIIVG VDRLDYIKGV
310 320 330 340 350
PQKLHAMEVF LNEHPEWRGK VVLVQVAVPS RGDVEEYQYL RSVVNELVGR
360 370 380 390 400
INGQFGTVEF VPIHFMHKSI PFEELISLYA VSDVCLVSST RDGMNLVSYE
410 420 430 440 450
YIACQEEKKG SLILSEFTGA AQSLNGAIIV NPWNTDDLSD AINEALTLPD
460 470 480 490
VKKEVNWEKL YKYISKYTSA FWGENFVHEL YSTSSSSTSS SATKN
Sequence cautioni
The sequence AAA66891 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | K → M in CAA48296 (PubMed:1425702).Curated | 1 | |
Sequence conflicti | 101 | H → L in CAA48296 (PubMed:1425702).Curated | 1 | |
Sequence conflicti | 104 | G → W in CAA48296 (PubMed:1425702).Curated | 1 | |
Sequence conflicti | 129 – 130 | LA → FG in CAA43580 (PubMed:1315471).Curated | 2 | |
Sequence conflicti | 217 | T → S in CAA48296 (PubMed:1425702).Curated | 1 | |
Sequence conflicti | 288 | I → L in CAA48296 (PubMed:1425702).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04450 Genomic DNA Translation: AAA66891.1 Frameshift. X68214 mRNA Translation: CAA48296.1 X68496 Genomic DNA Translation: CAA48510.1 X67499 Genomic DNA Translation: CAA47834.1 X61275 Genomic DNA Translation: CAA43580.1 L21999 Genomic DNA Translation: AAA53672.1 X78993 Genomic DNA Translation: CAA55627.1 Sequence problems. X75891 Genomic DNA Translation: CAA53485.1 AF061037 mRNA Translation: AAC16974.1 AY598966 Genomic DNA Translation: AAT27376.1 AY598964 Genomic DNA Translation: AAT27374.1 Z35995 Genomic DNA Translation: CAA85083.1 AY693147 Genomic DNA Translation: AAT93166.1 BK006936 Genomic DNA Translation: DAA07243.1 |
PIRi | S34979 |
RefSeqi | NP_009684.1, NM_001178474.1 |
Genome annotation databases
EnsemblFungii | YBR126C_mRNA; YBR126C; YBR126C |
GeneIDi | 852423 |
KEGGi | sce:YBR126C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04450 Genomic DNA Translation: AAA66891.1 Frameshift. X68214 mRNA Translation: CAA48296.1 X68496 Genomic DNA Translation: CAA48510.1 X67499 Genomic DNA Translation: CAA47834.1 X61275 Genomic DNA Translation: CAA43580.1 L21999 Genomic DNA Translation: AAA53672.1 X78993 Genomic DNA Translation: CAA55627.1 Sequence problems. X75891 Genomic DNA Translation: CAA53485.1 AF061037 mRNA Translation: AAC16974.1 AY598966 Genomic DNA Translation: AAT27376.1 AY598964 Genomic DNA Translation: AAT27374.1 Z35995 Genomic DNA Translation: CAA85083.1 AY693147 Genomic DNA Translation: AAT93166.1 BK006936 Genomic DNA Translation: DAA07243.1 |
PIRi | S34979 |
RefSeqi | NP_009684.1, NM_001178474.1 |
3D structure databases
SMRi | Q00764 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32827, 482 interactors |
ComplexPortali | CPX-582, Trehalose-6-phosphate synthase/phosphatase variant 1 CPX-583, Trehalose-6-phosphate synthase/phosphatase variant 2 |
DIPi | DIP-744N |
IntActi | Q00764, 39 interactors |
MINTi | Q00764 |
STRINGi | 4932.YBR126C |
Protein family/group databases
CAZyi | GT20, Glycosyltransferase Family 20 |
PTM databases
CarbonylDBi | Q00764 |
iPTMneti | Q00764 |
Proteomic databases
MaxQBi | Q00764 |
PaxDbi | Q00764 |
PRIDEi | Q00764 |
TopDownProteomicsi | Q00764 |
Genome annotation databases
EnsemblFungii | YBR126C_mRNA; YBR126C; YBR126C |
GeneIDi | 852423 |
KEGGi | sce:YBR126C |
Organism-specific databases
EuPathDBi | FungiDB:YBR126C |
SGDi | S000000330, TPS1 |
Phylogenomic databases
eggNOGi | KOG1050, Eukaryota |
GeneTreei | ENSGT00940000167933 |
HOGENOMi | CLU_002351_7_2_1 |
InParanoidi | Q00764 |
OMAi | YIACQQE |
Enzyme and pathway databases
BioCyci | MetaCyc:YBR126C-MONOMER |
BRENDAi | 2.4.1.15, 984 |
Miscellaneous databases
PROi | PR:Q00764 |
RNActi | Q00764, protein |
Family and domain databases
InterProi | View protein in InterPro IPR001830, Glyco_trans_20 IPR012766, Trehalose_OtsA |
Pfami | View protein in Pfam PF00982, Glyco_transf_20, 1 hit |
TIGRFAMsi | TIGR02400, trehalose_OtsA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TPS1_YEAST | |
Accessioni | Q00764Primary (citable) accession number: Q00764 Secondary accession number(s): D6VQC3 Q6J5J4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | July 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 200 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names