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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene

PLCB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.1 Publication

Miscellaneous

The receptor-mediated activation of PLC-beta-2 is most effectively mediated by one G-protein alpha subunit, alpha-16.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+Note: Binds 1 Ca2+ ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei327PROSITE-ProRule annotation1
Metal bindingi328Calcium1
Metal bindingi357Calcium1
Metal bindingi359Calcium1
Active sitei374PROSITE-ProRule annotation1
Metal bindingi408Calcium1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • phosphatidylinositol phospholipase C activity Source: AgBase
  • phospholipase C activity Source: Reactome

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS06408-MONOMER
BRENDAi3.1.4.11 2681
ReactomeiR-HSA-112043 PLC beta mediated events
R-HSA-1855204 Synthesis of IP3 and IP4 in the cytosol
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-4086398 Ca2+ pathway
R-HSA-416476 G alpha (q) signalling events
R-HSA-418217 G beta:gamma signalling through PLC beta
R-HSA-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-HSA-500657 Presynaptic function of Kainate receptors
SABIO-RKiQ00722
SignaLinkiQ00722
SIGNORiQ00722

Chemistry databases

SwissLipidsiSLP:000000948

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-2
Phospholipase C-beta-2
Short name:
PLC-beta-2
Gene namesi
Name:PLCB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000137841.11
HGNCiHGNC:9055 PLCB2
MIMi604114 gene
neXtProtiNX_Q00722

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52Q → A: Strongly reduces interaction with RAC1. 1 Publication1

Organism-specific databases

DisGeNETi5330
OpenTargetsiENSG00000137841
PharmGKBiPA33385

Polymorphism and mutation databases

BioMutaiPLCB2
DMDMi215273902

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000884891 – 11851-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2Add BLAST1185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei953PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ00722
MaxQBiQ00722
PaxDbiQ00722
PeptideAtlasiQ00722
PRIDEiQ00722
ProteomicsDBi57870
57871 [Q00722-2]

PTM databases

iPTMnetiQ00722
PhosphoSitePlusiQ00722

Expressioni

Gene expression databases

BgeeiENSG00000137841 Expressed in 149 organ(s), highest expression level in blood
CleanExiHS_PLCB2
ExpressionAtlasiQ00722 baseline and differential
GenevisibleiQ00722 HS

Organism-specific databases

HPAiCAB009443
HPA041298

Interactioni

Subunit structurei

Interacts with RAC1 (PubMed:1644792). Forms a complex composed of at least WDR26, a G-beta:gamma unit, and PLCB2 (PubMed:23625927).2 Publications

Protein-protein interaction databases

BioGridi111346, 13 interactors
DIPiDIP-29259N
IntActiQ00722, 1 interactor
STRINGi9606.ENSP00000260402

Structurei

Secondary structure

11185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ00722
SMRiQ00722
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ00722

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini312 – 463PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST152
Domaini546 – 662PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini669 – 767C2PROSITE-ProRule annotationAdd BLAST99

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili988 – 1147Sequence analysisAdd BLAST160

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi500 – 545Glu-richAdd BLAST46

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiQ00722
KOiK05858
OMAiDEIFTSH
OrthoDBiEOG091G00XL
PhylomeDBiQ00722
TreeFamiTF313216

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR028403 PLC-beta2
IPR014815 PLC-beta_C
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF10 PTHR10336:SF10, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF08703 PLC-beta_C, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q00722-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLLNPVLLP PKVKAYLSQG ERFIKWDDET TVASPVILRV DPKGYYLYWT
60 70 80 90 100
YQSKEMEFLD ITSIRDTRFG KFAKMPKSQK LRDVFNMDFP DNSFLLKTLT
110 120 130 140 150
VVSGPDMVDL TFHNFVSYKE NVGKAWAEDV LALVKHPLTA NASRSTFLDK
160 170 180 190 200
ILVKLKMQLN SEGKIPVKNF FQMFPADRKR VEAALSACHL PKGKNDAINP
210 220 230 240 250
EDFPEPVYKS FLMSLCPRPE IDEIFTSYHA KAKPYMTKEH LTKFINQKQR
260 270 280 290 300
DSRLNSLLFP PARPDQVQGL IDKYEPSGIN AQRGQLSPEG MVWFLCGPEN
310 320 330 340 350
SVLAQDKLLL HHDMTQPLNH YFINSSHNTY LTAGQFSGLS SAEMYRQVLL
360 370 380 390 400
SGCRCVELDC WKGKPPDEEP IITHGFTMTT DIFFKEAIEA IAESAFKTSP
410 420 430 440 450
YPIILSFENH VDSPRQQAKM AEYCRTIFGD MLLTEPLEKF PLKPGVPLPS
460 470 480 490 500
PEDLRGKILI KNKKNQFSGP TSSSKDTGGE AEGSSPPSAP AGEGTVWAGE
510 520 530 540 550
EGTELEEEEV EEEEEEESGN LDEEEIKKMQ SDEGTAGLEV TAYEEMSSLV
560 570 580 590 600
NYIQPTKFVS FEFSAQKNRS YVISSFTELK AYDLLSKASV QFVDYNKRQM
610 620 630 640 650
SRIYPKGTRM DSSNYMPQMF WNAGCQMVAL NFQTMDLPMQ QNMAVFEFNG
660 670 680 690 700
QSGYLLKHEF MRRPDKQFNP FSVDRIDVVV ATTLSITVIS GQFLSERSVR
710 720 730 740 750
TYVEVELFGL PGDPKRRYRT KLSPSTNSIN PVWKEEPFVF EKILMPELAS
760 770 780 790 800
LRVAVMEEGN KFLGHRIIPI NALNSGYHHL CLHSESNMPL TMPALFIFLE
810 820 830 840 850
MKDYIPGAWA DLTVALANPI KFFSAHDTKS VKLKEAMGGL PEKPFPLASP
860 870 880 890 900
VASQVNGALA PTSNGSPAAR AGAREEAMKE AAEPRTASLE ELRELKGVVK
910 920 930 940 950
LQRRHEKELR ELERRGARRW EELLQRGAAQ LAELGPPGVG GVGACKLGPG
960 970 980 990 1000
KGSRKKRSLP REESAGAAPG EGPEGVDGRV RELKDRLELE LLRQGEEQYE
1010 1020 1030 1040 1050
CVLKRKEQHV AEQISKMMEL AREKQAAELK ALKETSENDT KEMKKKLETK
1060 1070 1080 1090 1100
RLERIQGMTK VTTDKMAQER LKREINNSHI QEVVQVIKQM TENLERHQEK
1110 1120 1130 1140 1150
LEEKQAACLE QIREMEKQFQ KEALAEYEAR MKGLEAEVKE SVRACLRTCF
1160 1170 1180
PSEAKDKPER ACECPPELCE QDPLIAKADA QESRL
Length:1,185
Mass (Da):134,024
Last modified:November 25, 2008 - v2
Checksum:iA0397309D943672A
GO
Isoform 2 (identifier: Q00722-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     492-495: Missing.

Show »
Length:1,181
Mass (Da):133,680
Checksum:i7A6889F204D17FA4
GO
Isoform 3 (identifier: Q00722-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     868-882: Missing.

Note: No experimental confirmation available.
Show »
Length:1,170
Mass (Da):132,511
Checksum:iBC9DC96247E5762D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YNI4H0YNI4_HUMAN
1-phosphatidylinositol 4,5-bisphosp...
PLCB2
74Annotation score:
H0YKR9H0YKR9_HUMAN
1-phosphatidylinositol 4,5-bisphosp...
PLCB2
87Annotation score:
Q9BVT6Q9BVT6_HUMAN
1-phosphatidylinositol 4,5-bisphosp...
PLCB2 hCG_38956
199Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119K → R in BAF84346 (PubMed:14702039).Curated1
Sequence conflicti1060K → R in BAF84346 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_047509324N → I. Corresponds to variant dbSNP:rs45628633Ensembl.1
Natural variantiVAR_047510598R → H. Corresponds to variant dbSNP:rs8025153Ensembl.1
Natural variantiVAR_047511664P → L. Corresponds to variant dbSNP:rs9972332Ensembl.1
Natural variantiVAR_047512712G → R. Corresponds to variant dbSNP:rs28395835Ensembl.1
Natural variantiVAR_0475131095E → G1 PublicationCorresponds to variant dbSNP:rs936212Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035770492 – 495Missing in isoform 2. 1 Publication4
Alternative sequenceiVSP_054490868 – 882Missing in isoform 3. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95678 mRNA Translation: AAA36453.1
AK291657 mRNA Translation: BAF84346.1
AC020658 Genomic DNA No translation available.
BC136467 mRNA Translation: AAI36468.1
CCDSiCCDS42020.1 [Q00722-1]
CCDS61591.1 [Q00722-3]
CCDS61592.1 [Q00722-2]
PIRiA43346
RefSeqiNP_001271226.1, NM_001284297.1 [Q00722-2]
NP_001271227.1, NM_001284298.1 [Q00722-3]
NP_004564.2, NM_004573.2 [Q00722-1]
UniGeneiHs.355888

Genome annotation databases

EnsembliENST00000260402; ENSP00000260402; ENSG00000137841 [Q00722-1]
ENST00000456256; ENSP00000411991; ENSG00000137841 [Q00722-3]
ENST00000557821; ENSP00000453975; ENSG00000137841 [Q00722-2]
GeneIDi5330
KEGGihsa:5330
UCSCiuc001zld.4 human [Q00722-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95678 mRNA Translation: AAA36453.1
AK291657 mRNA Translation: BAF84346.1
AC020658 Genomic DNA No translation available.
BC136467 mRNA Translation: AAI36468.1
CCDSiCCDS42020.1 [Q00722-1]
CCDS61591.1 [Q00722-3]
CCDS61592.1 [Q00722-2]
PIRiA43346
RefSeqiNP_001271226.1, NM_001284297.1 [Q00722-2]
NP_001271227.1, NM_001284298.1 [Q00722-3]
NP_004564.2, NM_004573.2 [Q00722-1]
UniGeneiHs.355888

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FJUX-ray2.20B1-803[»]
2ZKMX-ray1.62X1-803[»]
ProteinModelPortaliQ00722
SMRiQ00722
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111346, 13 interactors
DIPiDIP-29259N
IntActiQ00722, 1 interactor
STRINGi9606.ENSP00000260402

Chemistry databases

SwissLipidsiSLP:000000948

PTM databases

iPTMnetiQ00722
PhosphoSitePlusiQ00722

Polymorphism and mutation databases

BioMutaiPLCB2
DMDMi215273902

Proteomic databases

EPDiQ00722
MaxQBiQ00722
PaxDbiQ00722
PeptideAtlasiQ00722
PRIDEiQ00722
ProteomicsDBi57870
57871 [Q00722-2]

Protocols and materials databases

DNASUi5330
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260402; ENSP00000260402; ENSG00000137841 [Q00722-1]
ENST00000456256; ENSP00000411991; ENSG00000137841 [Q00722-3]
ENST00000557821; ENSP00000453975; ENSG00000137841 [Q00722-2]
GeneIDi5330
KEGGihsa:5330
UCSCiuc001zld.4 human [Q00722-1]

Organism-specific databases

CTDi5330
DisGeNETi5330
EuPathDBiHostDB:ENSG00000137841.11
GeneCardsiPLCB2
H-InvDBiHIX0012125
HGNCiHGNC:9055 PLCB2
HPAiCAB009443
HPA041298
MIMi604114 gene
neXtProtiNX_Q00722
OpenTargetsiENSG00000137841
PharmGKBiPA33385
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiQ00722
KOiK05858
OMAiDEIFTSH
OrthoDBiEOG091G00XL
PhylomeDBiQ00722
TreeFamiTF313216

Enzyme and pathway databases

BioCyciMetaCyc:HS06408-MONOMER
BRENDAi3.1.4.11 2681
ReactomeiR-HSA-112043 PLC beta mediated events
R-HSA-1855204 Synthesis of IP3 and IP4 in the cytosol
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-4086398 Ca2+ pathway
R-HSA-416476 G alpha (q) signalling events
R-HSA-418217 G beta:gamma signalling through PLC beta
R-HSA-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-HSA-500657 Presynaptic function of Kainate receptors
SABIO-RKiQ00722
SignaLinkiQ00722
SIGNORiQ00722

Miscellaneous databases

ChiTaRSiPLCB2 human
EvolutionaryTraceiQ00722
GeneWikiiPLCB2
GenomeRNAii5330
PROiPR:Q00722
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137841 Expressed in 149 organ(s), highest expression level in blood
CleanExiHS_PLCB2
ExpressionAtlasiQ00722 baseline and differential
GenevisibleiQ00722 HS

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR028403 PLC-beta2
IPR014815 PLC-beta_C
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF10 PTHR10336:SF10, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF08703 PLC-beta_C, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLCB2_HUMAN
AccessioniPrimary (citable) accession number: Q00722
Secondary accession number(s): A8K6J2, B9EGH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 25, 2008
Last modified: November 7, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
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