UniProtKB - Q00690 (LYAM2_MOUSE)
E-selectin
Sele
Functioni
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1 (PubMed:1375914).
May have a role in capillary morphogenesis.
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 101 | CalciumBy similarity | 1 | |
Metal bindingi | 103 | CalciumBy similarity | 1 | |
Metal bindingi | 109 | CalciumBy similarity | 1 | |
Metal bindingi | 126 | CalciumBy similarity | 1 | |
Metal bindingi | 127 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- oligosaccharide binding Source: MGI
- phospholipase binding Source: MGI
- sialic acid binding Source: MGI
- transmembrane signaling receptor activity Source: MGI
GO - Biological processi
- actin filament-based process Source: MGI
- activation of phospholipase C activity Source: MGI
- heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
- inflammatory response Source: MGI
- leukocyte cell-cell adhesion Source: MGI
- leukocyte tethering or rolling Source: MGI
- positive regulation of leukocyte migration Source: MGI
- positive regulation of leukocyte tethering or rolling Source: UniProtKB
- positive regulation of receptor internalization Source: MGI
- response to cytokine Source: GO_Central
- response to interleukin-1 Source: MGI
Keywordsi
Biological process | Cell adhesion |
Ligand | Calcium, Lectin, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-202733, Cell surface interactions at the vascular wall |
Names & Taxonomyi
Protein namesi | Recommended name: E-selectinAlternative name(s): CD62 antigen-like family member E Endothelial leukocyte adhesion molecule 11 Publication Short name: ELAM-1 Leukocyte-endothelial cell adhesion molecule 2 Short name: LECAM2 CD_antigen: CD62E |
Gene namesi | Name:Sele Synonyms:Elam-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:98278, Sele |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein By similarity
Cytoskeleton
- cortical cytoskeleton Source: MGI
Extracellular region or secreted
- extracellular space Source: MGI
Plasma Membrane
- caveola Source: MGI
- clathrin-coated pit Source: MGI
- external side of plasma membrane Source: GO_Central
- integral component of plasma membrane Source: MGI
Other locations
- membrane raft Source: MGI
- perinuclear region of cytoplasm Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 557 | ExtracellularSequence analysisAdd BLAST | 536 | |
Transmembranei | 558 – 579 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 580 – 612 | CytoplasmicSequence analysisAdd BLAST | 33 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | By similarityAdd BLAST | 21 | |
ChainiPRO_0000017493 | 22 – 612 | E-selectinAdd BLAST | 591 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 25 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 40 ↔ 138 | By similarity | ||
Disulfide bondi | 111 ↔ 130 | By similarity | ||
Disulfide bondi | 143 ↔ 154 | By similarity | ||
Glycosylationi | 145 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 148 ↔ 163 | By similarity | ||
Disulfide bondi | 165 ↔ 174 | By similarity | ||
Disulfide bondi | 180 ↔ 225 | By similarity | ||
Glycosylationi | 192 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 193 ↔ 206 | By similarity | ||
Glycosylationi | 203 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 210 ↔ 238 | By similarity | ||
Disulfide bondi | 243 ↔ 287 | By similarity | ||
Disulfide bondi | 256 ↔ 269 | By similarity | ||
Glycosylationi | 266 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 273 ↔ 300 | By similarity | ||
Disulfide bondi | 305 ↔ 350 | By similarity | ||
Glycosylationi | 333 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 336 ↔ 363 | By similarity | ||
Disulfide bondi | 368 ↔ 413 | By similarity | ||
Glycosylationi | 391 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 399 ↔ 426 | By similarity | ||
Disulfide bondi | 431 ↔ 476 | By similarity | ||
Disulfide bondi | 462 ↔ 489 | By similarity | ||
Disulfide bondi | 494 ↔ 535 | By similarity | ||
Glycosylationi | 504 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 521 ↔ 548 | By similarity | ||
Glycosylationi | 528 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q00690 |
PRIDEi | Q00690 |
ProteomicsDBi | 291975 |
PTM databases
GlyGeni | Q00690, 9 sites |
iPTMneti | Q00690 |
PhosphoSitePlusi | Q00690 |
Interactioni
Subunit structurei
Interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction.
By similarityGO - Molecular functioni
- phospholipase binding Source: MGI
Protein-protein interaction databases
DIPi | DIP-59328N |
IntActi | Q00690, 3 interactors |
STRINGi | 10090.ENSMUSP00000027874 |
Chemistry databases
BindingDBi | Q00690 |
Miscellaneous databases
RNActi | Q00690, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 139 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 118 | |
Domaini | 140 – 175 | EGF-likePROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 178 – 240 | Sushi 1PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 241 – 302 | Sushi 2PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 303 – 365 | Sushi 3PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 366 – 428 | Sushi 4PROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 430 – 491 | Sushi 5PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 492 – 550 | Sushi 6PROSITE-ProRule annotationAdd BLAST | 59 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 101 – 109 | Carbohydrate bindingBy similarity | 9 | |
Regioni | 113 – 118 | Carbohydrate bindingBy similarity | 6 | |
Regioni | 126 – 128 | Carbohydrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
EGF-like domain, Repeat, Signal, Sushi, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
InParanoidi | Q00690 |
OrthoDBi | 445079at2759 |
PhylomeDBi | Q00690 |
Family and domain databases
CDDi | cd00033, CCP, 6 hits cd03592, CLECT_selectins_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR016187, CTDL_fold IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR033991, Selectin_CTLD IPR002396, Selectin_superfamily IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00059, Lectin_C, 1 hit PF00084, Sushi, 6 hits |
PRINTSi | PR00343, SELECTIN |
SMARTi | View protein in SMART SM00032, CCP, 6 hits SM00034, CLECT, 1 hit SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit SSF57535, SSF57535, 6 hits |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS50923, SUSHI, 6 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MNASRFLSAL VFVLLAGEST AWYYNASSEL MTYDEASAYC QRDYTHLVAI
60 70 80 90 100
QNKEEINYLN SNLKHSPSYY WIGIRKVNNV WIWVGTGKPL TEEAQNWAPG
110 120 130 140 150
EPNNKQRNED CVEIYIQRTK DSGMWNDERC NKKKLALCYT ASCTNASCSG
160 170 180 190 200
HGECIETINS YTCKCHPGFL GPNCEQAVTC KPQEHPDYGS LNCSHPFGPF
210 220 230 240 250
SYNSSCSFGC KRGYLPSSME TTVRCTSSGE WSAPAPACHV VECEALTHPA
260 270 280 290 300
HGIRKCSSNP GSYPWNTTCT FDCVEGYRRV GAQNLQCTSS GIWDNETPSC
310 320 330 340 350
KAVTCDAIPQ PQNGFVSCSH STAGELAFKS SCNFTCEQSF TLQGPAQVEC
360 370 380 390 400
SAQGQWTPQI PVCKAVQCEA LSAPQQGNMK CLPSASGPFQ NGSSCEFSCE
410 420 430 440 450
EGFELKGSRR LQCGPRGEWD SKKPTCSAVK CDDVPRPQNG VMECAHATTG
460 470 480 490 500
EFTYKSSCAF QCNEGFSLHG SAQLECTSQG KWTQEVPSCQ VVQCPSLDVP
510 520 530 540 550
GKMNMSCSGT AVFGTVCEFT CPDDWTLNGS AVLTCGATGR WSGMPPTCEA
560 570 580 590 600
PVSPTRPLVV ALSAAGTSLL TSSSLLYLLM RYFRKKAKKF VPASSCQSLQ
610
SFENYHVPSY NV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ3U5F6 | Q3U5F6_MOUSE | E-selectin | Sele | 619 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80778 Genomic DNA Translation: AAA37547.1 M87862 mRNA Translation: AAA37577.1 Different initiation. |
PIRi | S23174, B42755 |
RefSeqi | NP_035475.1, NM_011345.2 XP_006496778.1, XM_006496715.2 |
Genome annotation databases
GeneIDi | 20339 |
KEGGi | mmu:20339 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding E-selectin |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80778 Genomic DNA Translation: AAA37547.1 M87862 mRNA Translation: AAA37577.1 Different initiation. |
PIRi | S23174, B42755 |
RefSeqi | NP_035475.1, NM_011345.2 XP_006496778.1, XM_006496715.2 |
3D structure databases
SMRi | Q00690 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-59328N |
IntActi | Q00690, 3 interactors |
STRINGi | 10090.ENSMUSP00000027874 |
Chemistry databases
BindingDBi | Q00690 |
ChEMBLi | CHEMBL2545 |
PTM databases
GlyGeni | Q00690, 9 sites |
iPTMneti | Q00690 |
PhosphoSitePlusi | Q00690 |
Proteomic databases
PaxDbi | Q00690 |
PRIDEi | Q00690 |
ProteomicsDBi | 291975 |
Protocols and materials databases
ABCDi | Q00690, 1 sequenced antibody |
DNASUi | 20339 |
Genome annotation databases
GeneIDi | 20339 |
KEGGi | mmu:20339 |
Organism-specific databases
CTDi | 6401 |
MGIi | MGI:98278, Sele |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
InParanoidi | Q00690 |
OrthoDBi | 445079at2759 |
PhylomeDBi | Q00690 |
Enzyme and pathway databases
Reactomei | R-MMU-202733, Cell surface interactions at the vascular wall |
Miscellaneous databases
BioGRID-ORCSi | 20339, 0 hits in 64 CRISPR screens |
PROi | PR:Q00690 |
RNActi | Q00690, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00033, CCP, 6 hits cd03592, CLECT_selectins_like, 1 hit |
Gene3Di | 3.10.100.10, 1 hit |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR016187, CTDL_fold IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR033991, Selectin_CTLD IPR002396, Selectin_superfamily IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00059, Lectin_C, 1 hit PF00084, Sushi, 6 hits |
PRINTSi | PR00343, SELECTIN |
SMARTi | View protein in SMART SM00032, CCP, 6 hits SM00034, CLECT, 1 hit SM00181, EGF, 1 hit SM00179, EGF_CA, 1 hit |
SUPFAMi | SSF56436, SSF56436, 1 hit SSF57535, SSF57535, 6 hits |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS50923, SUSHI, 6 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | LYAM2_MOUSE | |
Accessioni | Q00690Primary (citable) accession number: Q00690 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | February 23, 2022 | |
This is version 160 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families