UniProtKB - Q00684 (CDC14_YEAST)
Protein
Tyrosine-protein phosphatase CDC14
Gene
CDC14
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Protein phosphatase which antagonizes mitotic cyclin-dependent kinase CDC28, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Inhibits transcription of ribosomal genes (rDNA) during anaphase and controls segregation of nucleolus by facilitating condensin targeting to rDNA chromatin in anaphase. Dephosphorylates SIC1, a CDC28 inhibitor, and SWI5, a transcription factor for SIC1, and induces degradation of mitotic cyclins, likely by dephosphorylating the activator of mitotic cyclin degradation, CDH1. Dephosphorylates the microtubule bundling factor ASE1 which is required to define a centered and focused mitotic spindle midzone that can drive continuous spindle elongation. Dephosphorylates the anaphase-promoting complex inhibitor ACM1, leading to its degradation. Facilitates INN1-CYK3 complex formation which promotes cytokinesis through the dephosphorylation of CDC28-phosphosphorylated INN1. Reverts also the inhibitory CDC28 phosphorylation of CHS2 for endoplasmic reticulum export, ensuring that septum formation is contingent upon chromosome separation and exit from mitosis. Additional substrates for CDC14 are the formins BNI1 and BNR1, as well as CDC6, DBP2, DSN1, INCENP, KAR9, MCM3, ORC2, ORC6, SLD2, and SWI6. Activity is inhibited by interaction with NET1 which sequesters it to the nucleolus.30 Publications
Miscellaneous
Present with 8550 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphatePROSITE-ProRule annotation2 PublicationsEC:3.1.3.48PROSITE-ProRule annotation2 Publications
Kineticsi
- KM=4 mM for p-nitrophenyl phosphate1 Publication
pH dependencei
Optimum pH is 6.9.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 283 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- phosphoprotein phosphatase activity Source: SGD
- protein serine/threonine phosphatase activity Source: SGD
- protein tyrosine/serine/threonine phosphatase activity Source: InterPro
- protein tyrosine phosphatase activity Source: GO_Central
GO - Biological processi
- autophagy of mitochondrion Source: SGD
- cell division Source: UniProtKB-KW
- cellular response to osmotic stress Source: SGD
- chromosome segregation Source: SGD
- meiotic spindle disassembly Source: SGD
- microtubule cytoskeleton organization Source: GO_Central
- mitotic cell cycle Source: SGD
- mitotic cell cycle arrest Source: GO_Central
- peptidyl-serine dephosphorylation Source: SGD
- positive regulation of autophagosome assembly Source: SGD
- positive regulation of cytokinesis Source: SGD
- positive regulation of mitotic actomyosin contractile ring assembly Source: SGD
- protein dephosphorylation Source: SGD
- regulation of exit from mitosis Source: SGD
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Cell cycle, Cell division, Meiosis, Mitosis |
Enzyme and pathway databases
BioCyci | YEAST:G3O-30477-MONOMER |
Reactomei | R-SCE-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-SCE-5687128, MAPK6/MAPK4 signaling |
SABIO-RKi | Q00684 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CDC14 Synonyms:OAF3 Ordered Locus Names:YFR028C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YFR028C |
SGDi | S000001924, CDC14 |
Subcellular locationi
Nucleus
Other locations
Note: Sequestered in the nucleolus for most of the cell cycle by the nucleolar proteins NET1 and TOF2, and is released into the nucleus and cytoplasm during anaphase. CDC55 maintains CDC14 sequestration in the nucleolus during early meiosis, which is essential for the assembly of the meiosis I spindle. In anaphase, the CDC14 early anaphase release (FEAR) network (including CDC5, ESP1, and SLK19), and the mitotic exit network (including the DBF2-MOB1 complex) coordinately trigger the release of CDC14 from the nucleolus.
Cytoskeleton
- mitotic spindle Source: GO_Central
- mitotic spindle midzone Source: GO_Central
- mitotic spindle pole body Source: GO_Central
- spindle pole Source: GO_Central
- spindle pole body Source: SGD
Nucleus
- nucleolus Source: SGD
- nucleus Source: SGD
- RENT complex Source: SGD
Other locations
- cellular bud neck Source: UniProtKB-SubCell
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 253 | D → A: Inactivates catalytic activity and leads to substrate retention. 1 Publication | 1 | |
Mutagenesisi | 280 | A → V: Leads to temperature sensitivity. 1 Publication | 1 | |
Mutagenesisi | 283 | C → S: Inactivates catalytic activity and leads to substrate retention. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094875 | 1 – 551 | Tyrosine-protein phosphatase CDC14Add BLAST | 551 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 467 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q00684 |
PaxDbi | Q00684 |
PRIDEi | Q00684 |
PTM databases
iPTMneti | Q00684 |
Interactioni
Subunit structurei
Component of the RENT (regulator of nucleolar silencing and telophase) complex which is composed of at least NET1, CDC14 and SIR2.
Interacts with CDC5, CRM1, SIC1, TOF2 and UTP7.
7 PublicationsBinary interactionsi
Hide detailsQ00684
Protein-protein interaction databases
BioGRIDi | 31182, 531 interactors |
ComplexPortali | CPX-1669, RENT complex |
DIPi | DIP-5116N |
IntActi | Q00684, 179 interactors |
MINTi | Q00684 |
STRINGi | 4932.YFR028C |
Miscellaneous databases
RNActi | Q00684, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q00684 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 190 – 342 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 153 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1720, Eukaryota |
GeneTreei | ENSGT00940000170847 |
HOGENOMi | CLU_017787_1_2_1 |
InParanoidi | Q00684 |
OMAi | WSPHQVL |
Family and domain databases
Gene3Di | 3.90.190.10, 2 hits |
InterProi | View protein in InterPro IPR026070, CDC14 IPR029260, DSPn IPR000340, Dual-sp_phosphatase_cat-dom IPR029021, Prot-tyrosine_phosphatase-like IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
PANTHERi | PTHR23339:SF27, PTHR23339:SF27, 1 hit |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF14671, DSPn, 1 hit |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 2 hits |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
i Sequence
Sequence statusi: Complete.
Q00684-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRSVYLDNT IEFLRGRVYL GAYDYTPEDT DELVFFTVED AIFYNSFHLD
60 70 80 90 100
FGPMNIGHLY RFAVIFHEIL NDPENANKAV VFYSSASTRQ RANAACMLCC
110 120 130 140 150
YMILVQAWTP HQVLQPLAQV DPPFMPFRDA GYSNADFEIT IQDVVYGVWR
160 170 180 190 200
AKEKGLIDLH SFNLESYEKY EHVEFGDFNV LTPDFIAFAS PQEDHPKGYL
210 220 230 240 250
ATKSSHLNQP FKSVLNFFAN NNVQLVVRLN SHLYNKKHFE DIGIQHLDLI
260 270 280 290 300
FEDGTCPDLS IVKNFVGAAE TIIKRGGKIA VHCKAGLGRT GCLIGAHLIY
310 320 330 340 350
TYGFTANECI GFLRFIRPGM VVGPQQHWLY LHQNDFREWK YTTRISLKPS
360 370 380 390 400
EAIGGLYPLI SLEEYRLQKK KLKDDKRVAQ NNIEGELRDL TMTPPSNGHG
410 420 430 440 450
ALSARNSSQP STANNGSNSF KSSAVPQTSP GQPRKGQNGS NTIEDINNNR
460 470 480 490 500
NPTSHANRKV VIESNNSDDE SMQDTNGTSN HYPKVSRKKN DISSASSSRM
510 520 530 540 550
EDNEPSATNI NNAADDTILR QLLPKNRRVT SGRRTTSAAG GIRKISGSIK
K
Sequence cautioni
The sequence AAA34477 differs from that shown. Reason: Frameshift.Curated
The sequence CAA52971 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 118 | A → P in BAA09533 (PubMed:8668128).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M61194 Genomic DNA Translation: AAA34477.1 Frameshift. D55715 Genomic DNA Translation: BAA09533.1 D50617 Genomic DNA Translation: BAA09267.1 X75077 Genomic DNA Translation: CAA52971.1 Frameshift. BK006940 Genomic DNA Translation: DAA12468.1 |
PIRi | S56283 |
RefSeqi | NP_116684.3, NM_001179993.3 |
Genome annotation databases
EnsemblFungii | YFR028C_mRNA; YFR028C; YFR028C |
GeneIDi | 850585 |
KEGGi | sce:YFR028C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M61194 Genomic DNA Translation: AAA34477.1 Frameshift. D55715 Genomic DNA Translation: BAA09533.1 D50617 Genomic DNA Translation: BAA09267.1 X75077 Genomic DNA Translation: CAA52971.1 Frameshift. BK006940 Genomic DNA Translation: DAA12468.1 |
PIRi | S56283 |
RefSeqi | NP_116684.3, NM_001179993.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4ZJ7 | X-ray | 2.40 | B | 517-551 | [»] | |
5XW4 | X-ray | 1.85 | A/B | 1-374 | [»] | |
5XW5 | X-ray | 1.85 | A/B | 1-374 | [»] | |
6G84 | X-ray | 2.47 | A/B | 1-374 | [»] | |
6G85 | X-ray | 1.53 | A/B | 1-374 | [»] | |
6G86 | X-ray | 1.74 | A/B | 1-374 | [»] | |
SMRi | Q00684 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31182, 531 interactors |
ComplexPortali | CPX-1669, RENT complex |
DIPi | DIP-5116N |
IntActi | Q00684, 179 interactors |
MINTi | Q00684 |
STRINGi | 4932.YFR028C |
PTM databases
iPTMneti | Q00684 |
Proteomic databases
MaxQBi | Q00684 |
PaxDbi | Q00684 |
PRIDEi | Q00684 |
Genome annotation databases
EnsemblFungii | YFR028C_mRNA; YFR028C; YFR028C |
GeneIDi | 850585 |
KEGGi | sce:YFR028C |
Organism-specific databases
EuPathDBi | FungiDB:YFR028C |
SGDi | S000001924, CDC14 |
Phylogenomic databases
eggNOGi | KOG1720, Eukaryota |
GeneTreei | ENSGT00940000170847 |
HOGENOMi | CLU_017787_1_2_1 |
InParanoidi | Q00684 |
OMAi | WSPHQVL |
Enzyme and pathway databases
BioCyci | YEAST:G3O-30477-MONOMER |
Reactomei | R-SCE-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-SCE-5687128, MAPK6/MAPK4 signaling |
SABIO-RKi | Q00684 |
Miscellaneous databases
PROi | PR:Q00684 |
RNActi | Q00684, protein |
Family and domain databases
Gene3Di | 3.90.190.10, 2 hits |
InterProi | View protein in InterPro IPR026070, CDC14 IPR029260, DSPn IPR000340, Dual-sp_phosphatase_cat-dom IPR029021, Prot-tyrosine_phosphatase-like IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
PANTHERi | PTHR23339:SF27, PTHR23339:SF27, 1 hit |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit PF14671, DSPn, 1 hit |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 2 hits |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDC14_YEAST | |
Accessioni | Q00684Primary (citable) accession number: Q00684 Secondary accession number(s): D6VTQ8, Q05180, Q05673 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | November 1, 1995 | |
Last modified: | December 2, 2020 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD