UniProtKB - Q00623 (APOA1_MOUSE)
Apolipoprotein A-I
Apoa1
Functioni
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
GO - Molecular functioni
- amyloid-beta binding Source: MGI
- apolipoprotein A-I receptor binding Source: MGI
- apolipoprotein receptor binding Source: MGI
- chemorepellent activity Source: MGI
- cholesterol binding Source: MGI
- cholesterol transfer activity Source: MGI
- enzyme binding Source: MGI
- heat shock protein binding Source: MGI
- high-density lipoprotein particle binding Source: MGI
- high-density lipoprotein particle receptor binding Source: MGI
- identical protein binding Source: MGI
- lipase inhibitor activity Source: MGI
- lipid binding Source: MGI
- lipid transporter activity Source: MGI
- lipoprotein particle binding Source: MGI
- phosphatidylcholine binding Source: GO_Central
- phosphatidylcholine-sterol O-acyltransferase activator activity Source: MGI
- phospholipid binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- signaling receptor binding Source: MGI
GO - Biological processi
- adrenal gland development Source: MGI
- animal organ regeneration Source: MGI
- blood vessel endothelial cell migration Source: MGI
- cholesterol biosynthetic process Source: MGI
- cholesterol efflux Source: MGI
- cholesterol homeostasis Source: MGI
- cholesterol import Source: MGI
- cholesterol metabolic process Source: MGI
- cholesterol transport Source: MGI
- endothelial cell proliferation Source: MGI
- glucocorticoid metabolic process Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- high-density lipoprotein particle assembly Source: MGI
- high-density lipoprotein particle remodeling Source: MGI
- integrin-mediated signaling pathway Source: MGI
- lipid storage Source: MGI
- lipoprotein biosynthetic process Source: MGI
- lipoprotein metabolic process Source: GO_Central
- negative chemotaxis Source: MGI
- negative regulation of cell adhesion molecule production Source: MGI
- negative regulation of cytokine production involved in immune response Source: MGI
- negative regulation of heterotypic cell-cell adhesion Source: MGI
- negative regulation of hydrolase activity Source: MGI
- negative regulation of inflammatory response Source: MGI
- negative regulation of interleukin-1 beta production Source: MGI
- negative regulation of lipase activity Source: MGI
- negative regulation of response to cytokine stimulus Source: MGI
- negative regulation of tumor necrosis factor-mediated signaling pathway Source: MGI
- negative regulation of very-low-density lipoprotein particle remodeling Source: MGI
- peptidyl-methionine modification Source: UniProtKB
- phosphatidylcholine biosynthetic process Source: MGI
- phosphatidylcholine metabolic process Source: GO_Central
- phospholipid efflux Source: MGI
- phospholipid homeostasis Source: MGI
- phospholipid metabolic process Source: MGI
- phospholipid transport Source: MGI
- positive regulation of cholesterol efflux Source: UniProtKB
- positive regulation of cholesterol esterification Source: MGI
- positive regulation of fatty acid biosynthetic process Source: GO_Central
- positive regulation of hydrolase activity Source: MGI
- positive regulation of lipid biosynthetic process Source: GO_Central
- positive regulation of lipoprotein lipase activity Source: GO_Central
- positive regulation of phagocytosis Source: UniProtKB
- positive regulation of phospholipid efflux Source: UniProtKB
- positive regulation of Rho protein signal transduction Source: MGI
- positive regulation of stress fiber assembly Source: MGI
- positive regulation of substrate adhesion-dependent cell spreading Source: MGI
- positive regulation of triglyceride catabolic process Source: GO_Central
- protein oxidation Source: UniProtKB
- protein stabilization Source: UniProtKB
- regulation of Cdc42 protein signal transduction Source: MGI
- regulation of intestinal cholesterol absorption Source: MGI
- regulation of protein phosphorylation Source: MGI
- reverse cholesterol transport Source: MGI
- triglyceride homeostasis Source: MGI
- very-low-density lipoprotein particle remodeling Source: GO_Central
- vitamin transport Source: MGI
Keywordsi
Biological process | Cholesterol metabolism, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-1369062, ABC transporters in lipid homeostasis R-MMU-2168880, Scavenging of heme from plasma R-MMU-3000471, Scavenging by Class B Receptors R-MMU-3000480, Scavenging by Class A Receptors R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275, Post-translational protein phosphorylation R-MMU-8963888, Chylomicron assembly R-MMU-8963896, HDL assembly R-MMU-8963901, Chylomicron remodeling R-MMU-8964011, HDL clearance R-MMU-8964058, HDL remodeling R-MMU-9707616, Heme signaling R-MMU-975634, Retinoid metabolism and transport |
Names & Taxonomyi
Protein namesi | Recommended name: Apolipoprotein A-IShort name: Apo-AI Short name: ApoA-I Alternative name(s): Apolipoprotein A1 Cleaved into the following 2 chains: |
Gene namesi | Name:Apoa1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88049, Apoa1 |
VEuPathDBi | HostDB:ENSMUSG00000032083 |
Subcellular locationi
Extracellular region or secreted
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- chylomicron Source: GO_Central
- discoidal high-density lipoprotein particle Source: MGI
- extracellular region Source: MGI
- extracellular space Source: MGI
- high-density lipoprotein particle Source: MGI
- intermediate-density lipoprotein particle Source: MGI
- low-density lipoprotein particle Source: MGI
- spherical high-density lipoprotein particle Source: MGI
- very-low-density lipoprotein particle Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cell surface Source: MGI
- cytoplasmic vesicle Source: MGI
- endocytic vesicle Source: MGI
Keywords - Cellular componenti
HDL, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | By similarityAdd BLAST | 18 | |
ChainiPRO_0000425326 | 19 – 264 | Proapolipoprotein A-IAdd BLAST | 246 | |
ChainiPRO_0000001946 | 25 – 264 | Apolipoprotein A-IAdd BLAST | 240 | |
ChainiPRO_0000416576 | 25 – 263 | Truncated apolipoprotein A-IBy similarityAdd BLAST | 239 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 109 | Methionine sulfoxideBy similarity | 1 | |
Modified residuei | 193 | Methionine sulfoxide1 Publication | 1 | |
Modified residuei | 240 | Methionine sulfoxide1 Publication | 1 | |
Modified residuei | 242 | Methionine sulfoxide1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Lipoprotein, Oxidation, Palmitate, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3892 |
jPOSTi | Q00623 |
PaxDbi | Q00623 |
PeptideAtlasi | Q00623 |
PRIDEi | Q00623 |
ProteomicsDBi | 281800 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00121209 Q00623 |
SWISS-2DPAGEi | Q00623 |
PTM databases
iPTMneti | Q00623 |
PhosphoSitePlusi | Q00623 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000032083, Expressed in liver and 137 other tissues |
ExpressionAtlasi | Q00623, baseline and differential |
Genevisiblei | Q00623, MM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with APOA1BP and CLU.
Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin.
Interacts with NDRG1.
Interacts with SCGB3A2 (By similarity).
Interacts with NAXE and YJEFN3 (By similarity).
By similarityBinary interactionsi
Q00623
With | #Exp. | IntAct |
---|---|---|
Kcnma1 [Q08460] | 4 | EBI-1634106,EBI-1633915 |
GO - Molecular functioni
- apolipoprotein A-I receptor binding Source: MGI
- apolipoprotein receptor binding Source: MGI
- chemorepellent activity Source: MGI
- enzyme binding Source: MGI
- heat shock protein binding Source: MGI
- high-density lipoprotein particle receptor binding Source: MGI
- identical protein binding Source: MGI
- protein homodimerization activity Source: UniProtKB
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 198155, 30 interactors |
CORUMi | Q00623 |
IntActi | Q00623, 3 interactors |
STRINGi | 10090.ENSMUSP00000034588 |
Miscellaneous databases
RNActi | Q00623, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q00623 |
SASBDBi | Q00623 |
SMRi | Q00623 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 67 – 88 | 1Add BLAST | 22 | |
Repeati | 89 – 110 | 2Add BLAST | 22 | |
Repeati | 111 – 121 | 3; half-lengthAdd BLAST | 11 | |
Repeati | 122 – 143 | 4Add BLAST | 22 | |
Repeati | 144 – 165 | 5Add BLAST | 22 | |
Repeati | 166 – 187 | 6Add BLAST | 22 | |
Repeati | 188 – 207 | 7; truncatedAdd BLAST | 20 | |
Repeati | 208 – 229 | 8Add BLAST | 22 | |
Repeati | 230 – 240 | 9; half-lengthAdd BLAST | 11 | |
Repeati | 241 – 264 | 10Add BLAST | 24 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 67 – 264 | 10 X approximate tandem repeatsAdd BLAST | 198 |
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502S1XQ, Eukaryota |
GeneTreei | ENSGT00950000182929 |
HOGENOMi | CLU_058447_1_0_1 |
InParanoidi | Q00623 |
OMAi | KEVREMW |
OrthoDBi | 1553412at2759 |
PhylomeDBi | Q00623 |
TreeFami | TF334458 |
Family and domain databases
InterProi | View protein in InterPro IPR000074, ApoA_E |
Pfami | View protein in Pfam PF01442, Apolipoprotein, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MKAVVLAVAL VFLTGSQAWH VWQQDEPQSQ WDKVKDFANV YVDAVKDSGR
60 70 80 90 100
DYVSQFESSS LGQQLNLNLL ENWDTLGSTV SQLQERLGPL TRDFWDNLEK
110 120 130 140 150
ETDWVRQEMN KDLEEVKQKV QPYLDEFQKK WKEDVELYRQ KVAPLGAELQ
160 170 180 190 200
ESARQKLQEL QGRLSPVAEE FRDRMRTHVD SLRTQLAPHS EQMRESLAQR
210 220 230 240 250
LAELKSNPTL NEYHTRAKTH LKTLGEKARP ALEDLRHSLM PMLETLKTQV
260
QSVIDKASET LTAQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1STX7 | A0A1L1STX7_MOUSE | Apolipoprotein A-I | Apoa1 | 122 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 143 | A → G in AAB58424 (Ref. 3) Curated | 1 | |
Sequence conflicti | 143 | A → G in AAB58425 (Ref. 3) Curated | 1 | |
Sequence conflicti | 143 | A → G in AAB58426 (Ref. 3) Curated | 1 | |
Sequence conflicti | 143 | A → G in AAB58427 (Ref. 3) Curated | 1 | |
Sequence conflicti | 146 | Missing in AAB58424 (Ref. 3) Curated | 1 | |
Sequence conflicti | 146 | Missing in AAB58425 (Ref. 3) Curated | 1 | |
Sequence conflicti | 146 | Missing in AAB58426 (Ref. 3) Curated | 1 | |
Sequence conflicti | 146 | Missing in AAB58427 (Ref. 3) Curated | 1 |
Mass spectrometryi
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 249 – 250 | QV → KA in strain: BALB/c, C3H and ICR. | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64262 mRNA Translation: CAA45560.1 X64263 Genomic DNA Translation: CAA45561.1 L04149 Genomic DNA No translation available. L04151 mRNA No translation available. U79572 Genomic DNA Translation: AAB58424.1 U79574 Genomic DNA Translation: AAB58426.1 U79573 Genomic DNA Translation: AAB58425.1 U79575 Genomic DNA Translation: AAB58427.1 AK076187 mRNA Translation: BAC36241.1 AK149576 mRNA Translation: BAE28968.1 AK161536 mRNA Translation: BAE36448.1 AC116503 Genomic DNA No translation available. BC012253 mRNA Translation: AAH12253.1 BC019837 mRNA Translation: AAH19837.1 |
CCDSi | CCDS40610.1 |
PIRi | S22420 |
RefSeqi | NP_033822.2, NM_009692.4 |
Genome annotation databases
Ensembli | ENSMUST00000034588; ENSMUSP00000034588; ENSMUSG00000032083 |
GeneIDi | 11806 |
KEGGi | mmu:11806 |
UCSCi | uc009phb.3, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X64262 mRNA Translation: CAA45560.1 X64263 Genomic DNA Translation: CAA45561.1 L04149 Genomic DNA No translation available. L04151 mRNA No translation available. U79572 Genomic DNA Translation: AAB58424.1 U79574 Genomic DNA Translation: AAB58426.1 U79573 Genomic DNA Translation: AAB58425.1 U79575 Genomic DNA Translation: AAB58427.1 AK076187 mRNA Translation: BAC36241.1 AK149576 mRNA Translation: BAE28968.1 AK161536 mRNA Translation: BAE36448.1 AC116503 Genomic DNA No translation available. BC012253 mRNA Translation: AAH12253.1 BC019837 mRNA Translation: AAH19837.1 |
CCDSi | CCDS40610.1 |
PIRi | S22420 |
RefSeqi | NP_033822.2, NM_009692.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LEM | NMR | - | A | 25-240 | [»] | |
AlphaFoldDBi | Q00623 | |||||
SASBDBi | Q00623 | |||||
SMRi | Q00623 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198155, 30 interactors |
CORUMi | Q00623 |
IntActi | Q00623, 3 interactors |
STRINGi | 10090.ENSMUSP00000034588 |
PTM databases
iPTMneti | Q00623 |
PhosphoSitePlusi | Q00623 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00121209 Q00623 |
SWISS-2DPAGEi | Q00623 |
Proteomic databases
CPTACi | non-CPTAC-3892 |
jPOSTi | Q00623 |
PaxDbi | Q00623 |
PeptideAtlasi | Q00623 |
PRIDEi | Q00623 |
ProteomicsDBi | 281800 |
Protocols and materials databases
Antibodypediai | 32291, 1601 antibodies from 48 providers |
DNASUi | 11806 |
Genome annotation databases
Ensembli | ENSMUST00000034588; ENSMUSP00000034588; ENSMUSG00000032083 |
GeneIDi | 11806 |
KEGGi | mmu:11806 |
UCSCi | uc009phb.3, mouse |
Organism-specific databases
CTDi | 335 |
MGIi | MGI:88049, Apoa1 |
VEuPathDBi | HostDB:ENSMUSG00000032083 |
Phylogenomic databases
eggNOGi | ENOG502S1XQ, Eukaryota |
GeneTreei | ENSGT00950000182929 |
HOGENOMi | CLU_058447_1_0_1 |
InParanoidi | Q00623 |
OMAi | KEVREMW |
OrthoDBi | 1553412at2759 |
PhylomeDBi | Q00623 |
TreeFami | TF334458 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-1369062, ABC transporters in lipid homeostasis R-MMU-2168880, Scavenging of heme from plasma R-MMU-3000471, Scavenging by Class B Receptors R-MMU-3000480, Scavenging by Class A Receptors R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-8957275, Post-translational protein phosphorylation R-MMU-8963888, Chylomicron assembly R-MMU-8963896, HDL assembly R-MMU-8963901, Chylomicron remodeling R-MMU-8964011, HDL clearance R-MMU-8964058, HDL remodeling R-MMU-9707616, Heme signaling R-MMU-975634, Retinoid metabolism and transport |
Miscellaneous databases
BioGRID-ORCSi | 11806, 1 hit in 75 CRISPR screens |
ChiTaRSi | Apoa1, mouse |
PROi | PR:Q00623 |
RNActi | Q00623, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032083, Expressed in liver and 137 other tissues |
ExpressionAtlasi | Q00623, baseline and differential |
Genevisiblei | Q00623, MM |
Family and domain databases
InterProi | View protein in InterPro IPR000074, ApoA_E |
Pfami | View protein in Pfam PF01442, Apolipoprotein, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | APOA1_MOUSE | |
Accessioni | Q00623Primary (citable) accession number: Q00623 Secondary accession number(s): O08855, O09042, Q8BPD5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | October 3, 2012 | |
Last modified: | May 25, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families