Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucose-6-phosphate 1-dehydrogenase X

Gene

G6pdx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Miscellaneous

Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase X (G6pdx), Glucose-6-phosphate 1-dehydrogenase 2 (G6pd2), Glucose-6-phosphate 1-dehydrogenase (G6pdx), Glucose-6-phosphate 1-dehydrogenase (G6pd2), Glucose-6-phosphate 1-dehydrogenase (G6pd2), Glucose-6-phosphate 1-dehydrogenase (G6pdx), Glucose-6-phosphate 1-dehydrogenase (G6pdx)
  2. 6-phosphogluconolactonase (Pgls)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (Pgd), 6-phosphogluconate dehydrogenase, decarboxylating (Pgd), 6-phosphogluconate dehydrogenase, decarboxylating (Pgd), 6-phosphogluconate dehydrogenase, decarboxylating (Pgd)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72NADP 1By similarity1
Binding sitei147NADP 1By similarity1
Binding sitei171NADP 1; via carbonyl oxygenBy similarity1
Binding sitei171SubstrateBy similarity1
Binding sitei239SubstrateBy similarity1
Binding sitei258SubstrateBy similarity1
Active sitei263Proton acceptorBy similarity1
Binding sitei357NADP 2By similarity1
Binding sitei360SubstrateBy similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei366NADP 2By similarity1
Binding sitei370NADP 2By similarity1
Binding sitei393NADP 2By similarity1
Binding sitei395SubstrateBy similarity1
Binding sitei487NADP 2By similarity1
Binding sitei503NADP 2By similarity1
Binding sitei509NADP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45NADP 1By similarity8
Nucleotide bindingi401 – 403NADP 2By similarity3
Nucleotide bindingi421 – 423NADP 2By similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

ReactomeiR-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-71336 Pentose phosphate pathway
UniPathwayi
UPA00115;UER00408

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase X (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:G6pdx
Synonyms:G6pd, G6pd-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:105979 G6pdx

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000680852 – 515Glucose-6-phosphate 1-dehydrogenase XAdd BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei507PhosphotyrosineCombined sources1

Post-translational modificationi

Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ00612
PaxDbiQ00612
PeptideAtlasiQ00612
PRIDEiQ00612

2D gel databases

REPRODUCTION-2DPAGEiIPI00228385
Q00612

PTM databases

iPTMnetiQ00612
PhosphoSitePlusiQ00612
SwissPalmiQ00612

Expressioni

Gene expression databases

BgeeiENSMUSG00000031400 Expressed in 293 organ(s), highest expression level in secondary oocyte
CleanExiMM_G6PDX
ExpressionAtlasiQ00612 baseline and differential
GenevisibleiQ00612 MM

Interactioni

Subunit structurei

Homotetramer; dimer of dimers. Interacts with SIRT2; the interaction is enhanced by H2O2 treatment (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199790, 2 interactors
IntActiQ00612, 3 interactors
MINTiQ00612
STRINGi10090.ENSMUSP00000004327

Structurei

3D structure databases

ProteinModelPortaliQ00612
SMRiQ00612
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni201 – 205Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
COG0364 LUCA
GeneTreeiENSGT00530000063435
HOGENOMiHOG000046192
HOVERGENiHBG000856
InParanoidiQ00612
KOiK00036
OMAiVEICVYE
OrthoDBiEOG091G06FN
PhylomeDBiQ00612
TreeFamiTF300584

Family and domain databases

HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PIRSFiPIRSF000110 G6PD, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00871 zwf, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q00612-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP
60 70 80 90 100
TIWWLFRDGL LPEDTFIVGY ARSRLTVDDI RKQSEPFFKA TPEERPKLEE
110 120 130 140 150
FFARNSYVAG QYDDAASYKH LNSHMNALHQ GMQANRLFYL ALPPTVYEAV
160 170 180 190 200
TKNIQETCMS QTGWNRIIVE KPFGRDLQSS NQLSNHISSL FREDQIYRID
210 220 230 240 250
HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF
260 270 280 290 300
DEFGIIRDVM QNHLLQMLCL VAMEKPATTG SDDVRDEKVK VLKCISEVET
310 320 330 340 350
DNVVLGQYVG NPNGEGEAAN GYLDDPTVPH GSTTATFAAA VLYVENERWD
360 370 380 390 400
GVPFILRCGK ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV
410 420 430 440 450
YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM
460 470 480 490 500
HFVRSDELRE AWRIFTPLLH KIDREKPQPI PYVYGSRGPT EADELMKRVG
510
FQYEGTYKWV NPHKL
Length:515
Mass (Da):59,263
Last modified:March 20, 2007 - v3
Checksum:iC8AF0D4099B7AEC3
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3KG36A3KG36_MOUSE
Glucose-6-phosphate 1-dehydrogenase
G6pdx RP23-436K3.17-004
396Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24D → Y in CAA37679 (PubMed:1874446).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11911 mRNA Translation: CAA77967.1
AK088135 mRNA Translation: BAC40166.1
X53617 Genomic DNA Translation: CAA37679.1
U88533 Genomic DNA Translation: AAB52998.1
U88534 Genomic DNA Translation: AAB52999.1
CCDSiCCDS30232.1
PIRiA56686
RefSeqiNP_032088.1, NM_008062.2
UniGeneiMm.27210

Genome annotation databases

EnsembliENSMUST00000004327; ENSMUSP00000004327; ENSMUSG00000031400
GeneIDi14381
KEGGimmu:14381
UCSCiuc009toy.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11911 mRNA Translation: CAA77967.1
AK088135 mRNA Translation: BAC40166.1
X53617 Genomic DNA Translation: CAA37679.1
U88533 Genomic DNA Translation: AAB52998.1
U88534 Genomic DNA Translation: AAB52999.1
CCDSiCCDS30232.1
PIRiA56686
RefSeqiNP_032088.1, NM_008062.2
UniGeneiMm.27210

3D structure databases

ProteinModelPortaliQ00612
SMRiQ00612
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199790, 2 interactors
IntActiQ00612, 3 interactors
MINTiQ00612
STRINGi10090.ENSMUSP00000004327

PTM databases

iPTMnetiQ00612
PhosphoSitePlusiQ00612
SwissPalmiQ00612

2D gel databases

REPRODUCTION-2DPAGEiIPI00228385
Q00612

Proteomic databases

EPDiQ00612
PaxDbiQ00612
PeptideAtlasiQ00612
PRIDEiQ00612

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004327; ENSMUSP00000004327; ENSMUSG00000031400
GeneIDi14381
KEGGimmu:14381
UCSCiuc009toy.1 mouse

Organism-specific databases

CTDi14381
MGIiMGI:105979 G6pdx

Phylogenomic databases

eggNOGiKOG0563 Eukaryota
COG0364 LUCA
GeneTreeiENSGT00530000063435
HOGENOMiHOG000046192
HOVERGENiHBG000856
InParanoidiQ00612
KOiK00036
OMAiVEICVYE
OrthoDBiEOG091G06FN
PhylomeDBiQ00612
TreeFamiTF300584

Enzyme and pathway databases

UniPathwayi
UPA00115;UER00408

ReactomeiR-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-71336 Pentose phosphate pathway

Miscellaneous databases

PROiPR:Q00612
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031400 Expressed in 293 organ(s), highest expression level in secondary oocyte
CleanExiMM_G6PDX
ExpressionAtlasiQ00612 baseline and differential
GenevisibleiQ00612 MM

Family and domain databases

HAMAPiMF_00966 G6PD, 1 hit
InterProiView protein in InterPro
IPR001282 G6P_DH
IPR019796 G6P_DH_AS
IPR022675 G6P_DH_C
IPR022674 G6P_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23429 PTHR23429, 1 hit
PfamiView protein in Pfam
PF02781 G6PD_C, 1 hit
PF00479 G6PD_N, 1 hit
PIRSFiPIRSF000110 G6PD, 1 hit
PRINTSiPR00079 G6PDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00871 zwf, 1 hit
PROSITEiView protein in PROSITE
PS00069 G6P_DEHYDROGENASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD1_MOUSE
AccessioniPrimary (citable) accession number: Q00612
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 20, 2007
Last modified: September 12, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again