UniProtKB - Q00536 (CDK16_HUMAN)
Cyclin-dependent kinase 16
CDK16
Functioni
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 194 | ATPCurated | 1 | |
Active sitei | 286 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 171 – 179 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
- protein serine/threonine kinase activity Source: UniProtKB
GO - Biological processi
- exocytosis Source: UniProtKB
- growth hormone secretion Source: UniProtKB
- neuron projection development Source: UniProtKB
- protein phosphorylation Source: GO_Central
- regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
- regulation of transcription involved in G1/S transition of mitotic cell cycle Source: GO_Central
- spermatogenesis Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Differentiation, Spermatogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 2681 |
PathwayCommonsi | Q00536 |
SignaLinki | Q00536 |
SIGNORi | Q00536 |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclin-dependent kinase 16 (EC:2.7.11.22)Alternative name(s): Cell division protein kinase 16 PCTAIRE-motif protein kinase 1 Serine/threonine-protein kinase PCTAIRE-1 |
Gene namesi | Name:CDK16 Synonyms:PCTAIRE1, PCTK1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8749, CDK16 |
MIMi | 311550, gene |
neXtProti | NX_Q00536 |
VEuPathDBi | HostDB:ENSG00000102225.15 |
Subcellular locationi
Plasma membrane
Other locations
- Cytoplasm
- secretory vesicle
- synaptosome By similarity
Note: Colocalizes with insulin in pancreas islets. Recruited to the cell membrane by CCNY.
Cytoskeleton
- microtubule cytoskeleton Source: HPA
Cytosol
- cytosol Source: HPA
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: UniProtKB
- neuron projection Source: UniProtKB-SubCell
- synaptic vesicle Source: Ensembl
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 12 | S → A: Abolishes phosphorylation by BRSK2. Abolishes effect on insulin secretion. 1 Publication | 1 | |
Mutagenesisi | 119 | S → A: Strongly reduces interaction with CCNY. 1 Publication | 1 | |
Mutagenesisi | 153 | S → A: Constitutively activated, due to loss of an inhibitory phosphorylation site. Increases interaction with CCNY. 2 Publications | 1 | |
Mutagenesisi | 153 | S → D: Abolishes interaction with CCNY. 2 Publications | 1 | |
Mutagenesisi | 194 | K → A: Loss of kinase activity. Abolishes effect on insulin secretion. 2 Publications | 1 | |
Mutagenesisi | 194 | K → R: Loss of kinase activity. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 5127 |
OpenTargetsi | ENSG00000102225 |
PharmGKBi | PA33095 |
Miscellaneous databases
Pharosi | Q00536, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4597 |
DrugBanki | DB07766, (2Z,3E)-2,3'-biindole-2',3(1H,1'H)-dione 3-{O-[(3R)-3,4-dihydroxybutyl]oxime} DB12010, Fostamatinib |
DrugCentrali | Q00536 |
Genetic variation databases
BioMutai | CDK16 |
DMDMi | 266425 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086484 | 1 – 496 | Cyclin-dependent kinase 16Add BLAST | 496 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | Phosphoserine; by BRSK2Combined sources1 Publication | 1 | |
Modified residuei | 42 | PhosphoserineCombined sources | 1 | |
Modified residuei | 65 | PhosphoserineCombined sources | 1 | |
Modified residuei | 78 | PhosphoserineCombined sources | 1 | |
Modified residuei | 82 | PhosphoserineCombined sources | 1 | |
Modified residuei | 95 | Phosphoserine; by CDK5Combined sources | 1 | |
Modified residuei | 110 | PhosphoserineBy similarity | 1 | |
Modified residuei | 119 | PhosphoserineCombined sources | 1 | |
Modified residuei | 138 | PhosphoserineCombined sources | 1 | |
Modified residuei | 153 | PhosphoserineCombined sources | 1 | |
Modified residuei | 155 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q00536 |
jPOSTi | Q00536 |
MassIVEi | Q00536 |
MaxQBi | Q00536 |
PaxDbi | Q00536 |
PeptideAtlasi | Q00536 |
PRIDEi | Q00536 |
ProteomicsDBi | 57854 [Q00536-1] |
PTM databases
iPTMneti | Q00536 |
PhosphoSitePlusi | Q00536 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000102225, Expressed in right hemisphere of cerebellum and 233 other tissues |
ExpressionAtlasi | Q00536, baseline and differential |
Genevisiblei | Q00536, HS |
Organism-specific databases
HPAi | ENSG00000102225, Tissue enhanced (skeletal) |
Interactioni
Subunit structurei
Found in a complex containing CABLES1, CDK17 and TDRD7.
Interacts with YWHAH, YWHAQ and YWHAZ.
Interacts with NSF.
Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1 (By similarity).
Interacts with BRSK2.
Interacts with CCNY; this increases the CDK16 kinase activity.
By similarity3 PublicationsBinary interactionsi
Hide detailsQ00536
Isoform 3 [Q00536-3]
With | #Exp. | IntAct |
---|---|---|
CCND3 [P30281] | 3 | EBI-12401765,EBI-375013 |
ZBTB14 [O43829] | 3 | EBI-12401765,EBI-10176632 |
Protein-protein interaction databases
BioGRIDi | 111154, 101 interactors |
IntActi | Q00536, 39 interactors |
MINTi | Q00536 |
STRINGi | 9606.ENSP00000276052 |
Chemistry databases
BindingDBi | Q00536 |
Miscellaneous databases
RNActi | Q00536, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q00536 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 165 – 446 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 282 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000156963 |
InParanoidi | Q00536 |
OMAi | CSHSEAA |
OrthoDBi | 1010560at2759 |
PhylomeDBi | Q00536 |
TreeFami | TF106508 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDRMKKIKRQ LSMTLRGGRG IDKTNGAPEQ IGLDESGGGG GSDPGEAPTR
60 70 80 90 100
AAPGELRSAR GPLSSAPEIV HEDLKMGSDG ESDQASATSS DEVQSPVRVR
110 120 130 140 150
MRNHPPRKIS TEDINKRLSL PADIRLPEGY LEKLTLNSPI FDKPLSRRLR
160 170 180 190 200
RVSLSEIGFG KLETYIKLDK LGEGTYATVY KGKSKLTDNL VALKEIRLEH
210 220 230 240 250
EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF EYLDKDLKQY
260 270 280 290 300
LDDCGNIINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL
310 320 330 340 350
KLADFGLARA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS TQIDMWGVGC
360 370 380 390 400
IFYEMATGRP LFPGSTVEEQ LHFIFRILGT PTEETWPGIL SNEEFKTYNY
410 420 430 440 450
PKYRAEALLS HAPRLDSDGA DLLTKLLQFE GRNRISAEDA MKHPFFLSLG
460 470 480 490
ERIHKLPDTT SIFALKEIQL QKEASLRSSS MPDSGRPAFR VVDTEF
Computationally mapped potential isoform sequencesi
There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE5RGN0 | E5RGN0_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 393 | Annotation score: | ||
E5RJM2 | E5RJM2_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 189 | Annotation score: | ||
E5RIU4 | E5RIU4_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 153 | Annotation score: | ||
E5RIY8 | E5RIY8_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 150 | Annotation score: | ||
A0A087WZU2 | A0A087WZU2_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 448 | Annotation score: | ||
H0YBX5 | H0YBX5_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 152 | Annotation score: | ||
H0YAZ9 | H0YAZ9_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 260 | Annotation score: | ||
H0YC36 | H0YC36_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 84 | Annotation score: | ||
H0YC60 | H0YC60_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 166 | Annotation score: | ||
E5RGV7 | E5RGV7_HUMAN | Cyclin-dependent kinase 16 | CDK16 | 83 | Annotation score: | ||
There is more potential isoformShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 25 | N → D in BAH13564 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046134 | 1 | M → MPLYGRARDHVTHPSILGTR PGRPMAGPITAAVPEKICNG AFCSCSGAFPLDPNNPSLGP LPSISHLNLRTQIAM in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_046342 | 1 | M → MQSEIAM in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66363 mRNA Translation: CAA47006.1 BT006827 mRNA Translation: AAP35473.1 AK290145 mRNA Translation: BAF82834.1 AK301832 mRNA Translation: BAH13564.1 AL096791 Genomic DNA No translation available. AL513366 Genomic DNA No translation available. CH471164 Genomic DNA Translation: EAW59295.1 CH471164 Genomic DNA Translation: EAW59297.1 BC001048 mRNA Translation: AAH01048.1 Different initiation. BC015607 mRNA Translation: AAH15607.1 Different initiation. |
CCDSi | CCDS14276.1 [Q00536-1] CCDS48101.1 [Q00536-3] CCDS55408.1 [Q00536-2] |
PIRi | S23385 |
RefSeqi | NP_001163931.1, NM_001170460.1 [Q00536-2] NP_006192.1, NM_006201.4 [Q00536-1] NP_148978.2, NM_033018.3 [Q00536-3] XP_016885059.1, XM_017029570.1 [Q00536-1] XP_016885060.1, XM_017029571.1 [Q00536-1] XP_016885061.1, XM_017029572.1 [Q00536-1] XP_016885062.1, XM_017029573.1 [Q00536-1] |
Genome annotation databases
Ensembli | ENST00000276052; ENSP00000276052; ENSG00000102225 [Q00536-2] ENST00000357227; ENSP00000349762; ENSG00000102225 [Q00536-1] ENST00000457458; ENSP00000405798; ENSG00000102225 [Q00536-3] ENST00000518022; ENSP00000429751; ENSG00000102225 [Q00536-1] |
GeneIDi | 5127 |
KEGGi | hsa:5127 |
UCSCi | uc004dho.4, human [Q00536-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X66363 mRNA Translation: CAA47006.1 BT006827 mRNA Translation: AAP35473.1 AK290145 mRNA Translation: BAF82834.1 AK301832 mRNA Translation: BAH13564.1 AL096791 Genomic DNA No translation available. AL513366 Genomic DNA No translation available. CH471164 Genomic DNA Translation: EAW59295.1 CH471164 Genomic DNA Translation: EAW59297.1 BC001048 mRNA Translation: AAH01048.1 Different initiation. BC015607 mRNA Translation: AAH15607.1 Different initiation. |
CCDSi | CCDS14276.1 [Q00536-1] CCDS48101.1 [Q00536-3] CCDS55408.1 [Q00536-2] |
PIRi | S23385 |
RefSeqi | NP_001163931.1, NM_001170460.1 [Q00536-2] NP_006192.1, NM_006201.4 [Q00536-1] NP_148978.2, NM_033018.3 [Q00536-3] XP_016885059.1, XM_017029570.1 [Q00536-1] XP_016885060.1, XM_017029571.1 [Q00536-1] XP_016885061.1, XM_017029572.1 [Q00536-1] XP_016885062.1, XM_017029573.1 [Q00536-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3MTL | X-ray | 2.40 | A | 163-478 | [»] | |
5G6V | X-ray | 2.20 | A/B | 163-478 | [»] | |
SMRi | Q00536 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111154, 101 interactors |
IntActi | Q00536, 39 interactors |
MINTi | Q00536 |
STRINGi | 9606.ENSP00000276052 |
Chemistry databases
BindingDBi | Q00536 |
ChEMBLi | CHEMBL4597 |
DrugBanki | DB07766, (2Z,3E)-2,3'-biindole-2',3(1H,1'H)-dione 3-{O-[(3R)-3,4-dihydroxybutyl]oxime} DB12010, Fostamatinib |
DrugCentrali | Q00536 |
PTM databases
iPTMneti | Q00536 |
PhosphoSitePlusi | Q00536 |
Genetic variation databases
BioMutai | CDK16 |
DMDMi | 266425 |
Proteomic databases
EPDi | Q00536 |
jPOSTi | Q00536 |
MassIVEi | Q00536 |
MaxQBi | Q00536 |
PaxDbi | Q00536 |
PeptideAtlasi | Q00536 |
PRIDEi | Q00536 |
ProteomicsDBi | 57854 [Q00536-1] |
Protocols and materials databases
Antibodypediai | 374, 216 antibodies |
DNASUi | 5127 |
Genome annotation databases
Ensembli | ENST00000276052; ENSP00000276052; ENSG00000102225 [Q00536-2] ENST00000357227; ENSP00000349762; ENSG00000102225 [Q00536-1] ENST00000457458; ENSP00000405798; ENSG00000102225 [Q00536-3] ENST00000518022; ENSP00000429751; ENSG00000102225 [Q00536-1] |
GeneIDi | 5127 |
KEGGi | hsa:5127 |
UCSCi | uc004dho.4, human [Q00536-1] |
Organism-specific databases
CTDi | 5127 |
DisGeNETi | 5127 |
GeneCardsi | CDK16 |
HGNCi | HGNC:8749, CDK16 |
HPAi | ENSG00000102225, Tissue enhanced (skeletal) |
MIMi | 311550, gene |
neXtProti | NX_Q00536 |
OpenTargetsi | ENSG00000102225 |
PharmGKBi | PA33095 |
VEuPathDBi | HostDB:ENSG00000102225.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000156963 |
InParanoidi | Q00536 |
OMAi | CSHSEAA |
OrthoDBi | 1010560at2759 |
PhylomeDBi | Q00536 |
TreeFami | TF106508 |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 2681 |
PathwayCommonsi | Q00536 |
SignaLinki | Q00536 |
SIGNORi | Q00536 |
Miscellaneous databases
BioGRID-ORCSi | 5127, 4 hits in 533 CRISPR screens |
ChiTaRSi | CDK16, human |
GeneWikii | PCTK1 |
GenomeRNAii | 5127 |
Pharosi | Q00536, Tchem |
PROi | PR:Q00536 |
RNActi | Q00536, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000102225, Expressed in right hemisphere of cerebellum and 233 other tissues |
ExpressionAtlasi | Q00536, baseline and differential |
Genevisiblei | Q00536, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK16_HUMAN | |
Accessioni | Q00536Primary (citable) accession number: Q00536 Secondary accession number(s): A8K280 J3KQP7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | February 10, 2021 | |
This is version 202 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human and mouse protein kinases
Human and mouse protein kinases: classification and index