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Protein

Cyclin-dependent kinase 16

Gene

CDK16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (By similarity). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).By similarity3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei194ATPCurated1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi171 – 179ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • exocytosis Source: UniProtKB
  • growth hormone secretion Source: UniProtKB
  • neuron projection development Source: UniProtKB
  • protein phosphorylation Source: ProtInc
  • regulation of cell cycle Source: GOC
  • regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • spermatogenesis Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Spermatogenesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22 2681
SignaLinkiQ00536
SIGNORiQ00536

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 16 (EC:2.7.11.22)
Alternative name(s):
Cell division protein kinase 16
PCTAIRE-motif protein kinase 1
Serine/threonine-protein kinase PCTAIRE-1
Gene namesi
Name:CDK16
Synonyms:PCTAIRE1, PCTK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000102225.15
HGNCiHGNC:8749 CDK16
MIMi311550 gene
neXtProtiNX_Q00536

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi12S → A: Abolishes phosphorylation by BRSK2. Abolishes effect on insulin secretion. 1 Publication1
Mutagenesisi119S → A: Strongly reduces interaction with CCNY. 1 Publication1
Mutagenesisi153S → A: Constitutively activated, due to loss of an inhibitory phosphorylation site. Increases interaction with CCNY. 2 Publications1
Mutagenesisi153S → D: Abolishes interaction with CCNY. 2 Publications1
Mutagenesisi194K → A: Loss of kinase activity. Abolishes effect on insulin secretion. 2 Publications1
Mutagenesisi194K → R: Loss of kinase activity. 2 Publications1

Organism-specific databases

DisGeNETi5127
OpenTargetsiENSG00000102225
PharmGKBiPA33095

Chemistry databases

ChEMBLiCHEMBL4597

Polymorphism and mutation databases

BioMutaiCDK16
DMDMi266425

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864841 – 496Cyclin-dependent kinase 16Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12Phosphoserine; by BRSK2Combined sources1 Publication1
Modified residuei42PhosphoserineCombined sources1
Modified residuei65PhosphoserineCombined sources1
Modified residuei78PhosphoserineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei95Phosphoserine; by CDK5Combined sources1
Modified residuei110PhosphoserineBy similarity1
Modified residuei119PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei153PhosphoserineCombined sources1
Modified residuei155PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-153 inhibits kinase activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ00536
MaxQBiQ00536
PaxDbiQ00536
PeptideAtlasiQ00536
PRIDEiQ00536
ProteomicsDBi57854

PTM databases

iPTMnetiQ00536
PhosphoSitePlusiQ00536

Expressioni

Tissue specificityi

Detected in pancreas islets (at protein level). Detected in brain and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000102225 Expressed in 219 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_PCTK1
ExpressionAtlasiQ00536 baseline and differential
GenevisibleiQ00536 HS

Organism-specific databases

HPAiCAB016535
HPA000974
HPA001366

Interactioni

Subunit structurei

Found in a complex containing CABLES1, CDK17 and TDRD7. Interacts with YWHAH, YWHAQ and YWHAZ. Interacts with NSF. Identified in a complex with NSF, syntaxin-1, synaptotagmin, SYN1, SYP and CDK5R1 (By similarity). Interacts with BRSK2. Interacts with CCNY; this increases the CDK16 kinase activity.By similarity3 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi111154, 91 interactors
IntActiQ00536, 29 interactors
MINTiQ00536
STRINGi9606.ENSP00000276052

Chemistry databases

BindingDBiQ00536

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ00536
SMRiQ00536
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 446Protein kinasePROSITE-ProRule annotationAdd BLAST282

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
GeneTreeiENSGT00900000140869
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiQ00536
KOiK08820
OMAiGRGIDKT
OrthoDBiEOG091G0CH0
PhylomeDBiQ00536
TreeFamiTF106508

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q00536-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDRMKKIKRQ LSMTLRGGRG IDKTNGAPEQ IGLDESGGGG GSDPGEAPTR
60 70 80 90 100
AAPGELRSAR GPLSSAPEIV HEDLKMGSDG ESDQASATSS DEVQSPVRVR
110 120 130 140 150
MRNHPPRKIS TEDINKRLSL PADIRLPEGY LEKLTLNSPI FDKPLSRRLR
160 170 180 190 200
RVSLSEIGFG KLETYIKLDK LGEGTYATVY KGKSKLTDNL VALKEIRLEH
210 220 230 240 250
EEGAPCTAIR EVSLLKDLKH ANIVTLHDII HTEKSLTLVF EYLDKDLKQY
260 270 280 290 300
LDDCGNIINM HNVKLFLFQL LRGLAYCHRQ KVLHRDLKPQ NLLINERGEL
310 320 330 340 350
KLADFGLARA KSIPTKTYSN EVVTLWYRPP DILLGSTDYS TQIDMWGVGC
360 370 380 390 400
IFYEMATGRP LFPGSTVEEQ LHFIFRILGT PTEETWPGIL SNEEFKTYNY
410 420 430 440 450
PKYRAEALLS HAPRLDSDGA DLLTKLLQFE GRNRISAEDA MKHPFFLSLG
460 470 480 490
ERIHKLPDTT SIFALKEIQL QKEASLRSSS MPDSGRPAFR VVDTEF
Length:496
Mass (Da):55,716
Last modified:April 1, 1993 - v1
Checksum:i9A5DDD34A5E5CBBB
GO
Isoform 2 (identifier: Q00536-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPLYGRARDH...HLNLRTQIAM

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):63,458
Checksum:i9D3020F5CADD1899
GO
Isoform 3 (identifier: Q00536-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MQSEIAM

Note: No experimental confirmation available.
Show »
Length:502
Mass (Da):56,375
Checksum:iAC4B7353DFF4D005
GO

Computationally mapped potential isoform sequencesi

There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E5RGN0E5RGN0_HUMAN
Cyclin-dependent kinase 16
CDK16
393Annotation score:
E5RJM2E5RJM2_HUMAN
Cyclin-dependent kinase 16
CDK16
189Annotation score:
E5RIY8E5RIY8_HUMAN
Cyclin-dependent kinase 16
CDK16
150Annotation score:
E5RIU4E5RIU4_HUMAN
Cyclin-dependent kinase 16
CDK16
153Annotation score:
A0A087WZU2A0A087WZU2_HUMAN
Cyclin-dependent kinase 16
CDK16
448Annotation score:
H0YAZ9H0YAZ9_HUMAN
Cyclin-dependent kinase 16
CDK16
260Annotation score:
H0YC60H0YC60_HUMAN
Cyclin-dependent kinase 16
CDK16
166Annotation score:
H0YC36H0YC36_HUMAN
Cyclin-dependent kinase 16
CDK16
84Annotation score:
H0YBX5H0YBX5_HUMAN
Cyclin-dependent kinase 16
CDK16
152Annotation score:
E5RJQ8E5RJQ8_HUMAN
Cyclin-dependent kinase 16
CDK16
57Annotation score:
There is more potential isoformShow all

Sequence cautioni

The sequence AAH01048 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH15607 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25N → D in BAH13564 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0461341M → MPLYGRARDHVTHPSILGTR PGRPMAGPITAAVPEKICNG AFCSCSGAFPLDPNNPSLGP LPSISHLNLRTQIAM in isoform 2. 1 Publication1
Alternative sequenceiVSP_0463421M → MQSEIAM in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66363 mRNA Translation: CAA47006.1
BT006827 mRNA Translation: AAP35473.1
AK290145 mRNA Translation: BAF82834.1
AK301832 mRNA Translation: BAH13564.1
AL096791 Genomic DNA No translation available.
AL513366 Genomic DNA No translation available.
CH471164 Genomic DNA Translation: EAW59295.1
CH471164 Genomic DNA Translation: EAW59297.1
BC001048 mRNA Translation: AAH01048.1 Different initiation.
BC015607 mRNA Translation: AAH15607.1 Different initiation.
CCDSiCCDS14276.1 [Q00536-1]
CCDS48101.1 [Q00536-3]
CCDS55408.1 [Q00536-2]
PIRiS23385
RefSeqiNP_001163931.1, NM_001170460.1 [Q00536-2]
NP_006192.1, NM_006201.4 [Q00536-1]
NP_148978.2, NM_033018.3 [Q00536-3]
XP_016885059.1, XM_017029570.1 [Q00536-1]
XP_016885060.1, XM_017029571.1 [Q00536-1]
XP_016885061.1, XM_017029572.1 [Q00536-1]
XP_016885062.1, XM_017029573.1 [Q00536-1]
UniGeneiHs.496068

Genome annotation databases

EnsembliENST00000276052; ENSP00000276052; ENSG00000102225 [Q00536-2]
ENST00000357227; ENSP00000349762; ENSG00000102225 [Q00536-1]
ENST00000457458; ENSP00000405798; ENSG00000102225 [Q00536-3]
ENST00000518022; ENSP00000429751; ENSG00000102225 [Q00536-1]
GeneIDi5127
KEGGihsa:5127
UCSCiuc004dho.4 human [Q00536-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66363 mRNA Translation: CAA47006.1
BT006827 mRNA Translation: AAP35473.1
AK290145 mRNA Translation: BAF82834.1
AK301832 mRNA Translation: BAH13564.1
AL096791 Genomic DNA No translation available.
AL513366 Genomic DNA No translation available.
CH471164 Genomic DNA Translation: EAW59295.1
CH471164 Genomic DNA Translation: EAW59297.1
BC001048 mRNA Translation: AAH01048.1 Different initiation.
BC015607 mRNA Translation: AAH15607.1 Different initiation.
CCDSiCCDS14276.1 [Q00536-1]
CCDS48101.1 [Q00536-3]
CCDS55408.1 [Q00536-2]
PIRiS23385
RefSeqiNP_001163931.1, NM_001170460.1 [Q00536-2]
NP_006192.1, NM_006201.4 [Q00536-1]
NP_148978.2, NM_033018.3 [Q00536-3]
XP_016885059.1, XM_017029570.1 [Q00536-1]
XP_016885060.1, XM_017029571.1 [Q00536-1]
XP_016885061.1, XM_017029572.1 [Q00536-1]
XP_016885062.1, XM_017029573.1 [Q00536-1]
UniGeneiHs.496068

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MTLX-ray2.40A163-478[»]
5G6VX-ray2.20A/B163-478[»]
ProteinModelPortaliQ00536
SMRiQ00536
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111154, 91 interactors
IntActiQ00536, 29 interactors
MINTiQ00536
STRINGi9606.ENSP00000276052

Chemistry databases

BindingDBiQ00536
ChEMBLiCHEMBL4597

PTM databases

iPTMnetiQ00536
PhosphoSitePlusiQ00536

Polymorphism and mutation databases

BioMutaiCDK16
DMDMi266425

Proteomic databases

EPDiQ00536
MaxQBiQ00536
PaxDbiQ00536
PeptideAtlasiQ00536
PRIDEiQ00536
ProteomicsDBi57854

Protocols and materials databases

DNASUi5127
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276052; ENSP00000276052; ENSG00000102225 [Q00536-2]
ENST00000357227; ENSP00000349762; ENSG00000102225 [Q00536-1]
ENST00000457458; ENSP00000405798; ENSG00000102225 [Q00536-3]
ENST00000518022; ENSP00000429751; ENSG00000102225 [Q00536-1]
GeneIDi5127
KEGGihsa:5127
UCSCiuc004dho.4 human [Q00536-1]

Organism-specific databases

CTDi5127
DisGeNETi5127
EuPathDBiHostDB:ENSG00000102225.15
GeneCardsiCDK16
HGNCiHGNC:8749 CDK16
HPAiCAB016535
HPA000974
HPA001366
MIMi311550 gene
neXtProtiNX_Q00536
OpenTargetsiENSG00000102225
PharmGKBiPA33095
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0594 Eukaryota
ENOG410XPP3 LUCA
GeneTreeiENSGT00900000140869
HOGENOMiHOG000233024
HOVERGENiHBG014652
InParanoidiQ00536
KOiK08820
OMAiGRGIDKT
OrthoDBiEOG091G0CH0
PhylomeDBiQ00536
TreeFamiTF106508

Enzyme and pathway databases

BRENDAi2.7.11.22 2681
SignaLinkiQ00536
SIGNORiQ00536

Miscellaneous databases

ChiTaRSiCDK16 human
GeneWikiiPCTK1
GenomeRNAii5127
PROiPR:Q00536
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102225 Expressed in 219 organ(s), highest expression level in right hemisphere of cerebellum
CleanExiHS_PCTK1
ExpressionAtlasiQ00536 baseline and differential
GenevisibleiQ00536 HS

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCDK16_HUMAN
AccessioniPrimary (citable) accession number: Q00536
Secondary accession number(s): A8K280
, B7Z7C8, J3KN74, J3KQP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 12, 2018
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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