UniProtKB - Q00422 (GABPA_MOUSE)

BLAST

>sp|Q00422|GABPA_MOUSE GA-binding protein alpha chain OS=Mus musculus OX=10090 GN=Gabpa PE=1 SV=2
MTKREAEELIEIEIDGTEKAECTEESIVEQTYTPAECVSQAIDINEPIGNLKKLLEPRLQ
CSLDAHEICLQDIQLDPDRSLFDQGVKTDGTVQLSVQVISYQGMEPKLNILEIVKTAETV
EVVIDPDAHHAEAEAHLVEEAQVITLDGTKHITTISDETSEQVTRWAAALEGYRKEQERL
GIPYDPIHWSTDQVLHWVVWVMKEFSMTDIDLTTLNISGRELCSLNQEDFFQRVPRGEIL
WSHLELLRKYVLASQEQQMNEIVTIDQPVQIIPASVPPATPTTIKVINSSAKAAKVQRSP
RISGEDRSSPGNRTGNNGQIQLWQFLLELLTDKDARDCISWVGDEGEFKLNQPELVAQKW
GQRKNKPTMNYEKLSRALRYYYDGDMICKVQGKRFVYKFVCDLKTLIGYSAAELNRLVIE
CEQKKLARMQLHGIAQPVTAVALAATSLQADKEI

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History
Entry version 174 (07 Nov 2018)
Sequence version 2 (27 Jul 2011)
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Protein

GA-binding protein alpha chain

Gene

Gabpa

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Transcription factor capable of interacting with purine rich repeats (GA repeats).

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	320 – 400	ETSPROSITE-ProRule annotationAdd BLAST		81

GO - Molecular functionⁱ

activating transcription factor binding Source: MGI
chromatin binding
Source: MGI
Inferred from direct assayⁱ
- 16757682
DNA binding
Source: MGI
Inferred from direct assayⁱ
- 11004506
- 15361867
DNA-binding transcription activator activity, RNA polymerase II-specific
Source: MGI
Inferred from direct assayⁱ
- 22780989
DNA-binding transcription factor activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein heterodimerization activity
Source: MGI
Inferred from sequence orthologyⁱ
- 9857059
proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
transcription regulatory region DNA binding
Source: MGI
Inferred from sequence orthologyⁱ
- 9857059

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

blastocyst formation
Source: MGI
Inferred from mutant phenotypeⁱ
- 27869233
cell differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
cellular response to dopamine Source: MGI
in utero embryonic development
Source: MGI
Inferred from mutant phenotypeⁱ
- 17485447
negative regulation of megakaryocyte differentiation
Source: MGI
Inferred from mutant phenotypeⁱ
- 16757682
negative regulation of transcription by RNA polymerase II
Source: MGI
Inferred from mutant phenotypeⁱ
- 17485447
positive regulation of gene expression Source: MGI
positive regulation of transcription by RNA polymerase II
Source: MGI
Inferred from sequence orthologyⁱ
- 9857059
regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 15361867

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2151201 Transcriptional activation of mitochondrial biogenesis

Reactomeⁱ

R-MMU-2151201 Transcriptional activation of mitochondrial biogenesis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: GA-binding protein alpha chain Short name: GABP subunit alpha
Gene namesⁱ	Name:Gabpa Synonyms:E4tf1a
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:95610 Gabpa

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000204128	1 – 454	GA-binding protein alpha chainAdd BLAST		454

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	303	PhosphoserineBy similarity		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q00422
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q00422
PaxDbⁱ	Q00422
PeptideAtlas More...PeptideAtlasⁱ	Q00422
PRIDEⁱ	Q00422

PTM databases

iPTMnetⁱ	Q00422
PhosphoSitePlusⁱ	Q00422

Expressionⁱ

Tissue specificityⁱ

Ubiquitous.

Gene expression databases

Bgeeⁱ	ENSMUSG00000008976 Expressed in 322 organ(s), highest expression level in cleaving embryo
CleanExⁱ	MM_GABPA
Genevisibleⁱ	Q00422 MM

Interactionⁱ

Subunit structureⁱ

Heterotetramer of two alpha and two beta subunits.

GO - Molecular functionⁱ

activating transcription factor binding Source: MGI
protein heterodimerization activity
Source: MGI
Inferred from sequence orthologyⁱ
- 9857059

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	199795, 1 interactor
Database of interacting proteins More...DIPⁱ	DIP-156N
IntActⁱ	Q00422, 1 interactor
Molecular INTeraction database More...MINTⁱ	Q00422
STRINGⁱ	10090.ENSMUSP00000009120

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	36 – 47	Combined sources	12
Helixⁱ	48 – 50	Combined sources	3
Helixⁱ	51 – 54	Combined sources	4
Helixⁱ	56 – 59	Combined sources	4
Beta strandⁱ	67 – 70	Combined sources	4
Turnⁱ	81 – 83	Combined sources	3
Beta strandⁱ	90 – 99	Combined sources	10
Beta strandⁱ	107 – 115	Combined sources	9
Helixⁱ	168 – 177	Combined sources	10
Turnⁱ	178 – 180	Combined sources	3
Helixⁱ	186 – 188	Combined sources	3
Helixⁱ	191 – 204	Combined sources	14
Helixⁱ	213 – 215	Combined sources	3
Helixⁱ	219 – 224	Combined sources	6
Helixⁱ	227 – 233	Combined sources	7
Helixⁱ	238 – 249	Combined sources	12
Turnⁱ	250 – 252	Combined sources	3
Helixⁱ	322 – 330	Combined sources	9
Turnⁱ	333 – 338	Combined sources	6
Beta strandⁱ	339 – 341	Combined sources	3
Beta strandⁱ	343 – 349	Combined sources	7
Helixⁱ	353 – 364	Combined sources	12
Helixⁱ	371 – 379	Combined sources	9
Helixⁱ	380 – 383	Combined sources	4
Beta strandⁱ	384 – 389	Combined sources	6
Beta strandⁱ	396 – 399	Combined sources	4
Helixⁱ	403 – 406	Combined sources	4
Helixⁱ	411 – 428	Combined sources	18

Show more details

3D structure databases

ProteinModelPortalⁱ	Q00422
SMRⁱ	Q00422
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q00422

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	168 – 251	PNTPROSITE-ProRule annotationAdd BLAST		84

Sequence similaritiesⁱ

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3806 Eukaryota ENOG410Z0ZF LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118907
HOGENOMⁱ	HOG000285952
HOVERGENⁱ	HBG051687
InParanoidⁱ	Q00422
KEGG Orthology (KO) More...KOⁱ	K09441
OMAⁱ	NVFWTHL
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0EXE
TreeFamⁱ	TF350537

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR000418 Ets_dom IPR024668 GABP_asu_N IPR003118 Pointed_dom IPR013761 SAM/pointed_sf IPR016312 TF_GA-bd_asu IPR029071 Ubiquitin-like_domsf IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00178 Ets, 1 hit PF11620 GABP-alpha, 1 hit PF02198 SAM_PNT, 1 hit
PIRSFⁱ	PIRSF001703 GABP_alpha, 1 hit
PRINTSⁱ	PR00454 ETSDOMAIN
SMARTⁱ	View protein in SMART SM00413 ETS, 1 hit SM00251 SAM_PNT, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 1 hit SSF47769 SSF47769, 1 hit SSF54236 SSF54236, 1 hit
PROSITEⁱ	View protein in PROSITE PS00345 ETS_DOMAIN_1, 1 hit PS00346 ETS_DOMAIN_2, 1 hit PS50061 ETS_DOMAIN_3, 1 hit PS51433 PNT, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q00422-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYTPAECVSQ AIDINEPIGN 
        60         70         80         90        100
LKKLLEPRLQ CSLDAHEICL QDIQLDPDRS LFDQGVKTDG TVQLSVQVIS 
       110        120        130        140        150
YQGMEPKLNI LEIVKTAETV EVVIDPDAHH AEAEAHLVEE AQVITLDGTK 
       160        170        180        190        200
HITTISDETS EQVTRWAAAL EGYRKEQERL GIPYDPIHWS TDQVLHWVVW 
       210        220        230        240        250
VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL WSHLELLRKY 
       260        270        280        290        300
VLASQEQQMN EIVTIDQPVQ IIPASVPPAT PTTIKVINSS AKAAKVQRSP 
       310        320        330        340        350
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL 
       360        370        380        390        400
NQPELVAQKW GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV 
       410        420        430        440        450
CDLKTLIGYS AAELNRLVIE CEQKKLARMQ LHGIAQPVTA VALAATSLQA 

DKEI

Length:454

Mass (Da):51,344

Last modified:July 27, 2011 - v2

Checksum:ⁱ2CBC40B895563685

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	188	H → R in AAA53030 (PubMed:1876836).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M74515 mRNA Translation: AAA53030.1 AK139916 mRNA Translation: BAE24181.1 AK145446 mRNA Translation: BAE26442.1 AK145838 mRNA Translation: BAE26687.1 AK148087 mRNA Translation: BAE28337.1 AK150455 mRNA Translation: BAE29575.1 CH466521 Genomic DNA Translation: EDK98317.1 BC052448 mRNA Translation: AAH52448.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28284.1
PIRⁱ	A40858
NCBI Reference Sequences More...RefSeqⁱ	NP_032091.2, NM_008065.2 XP_006522970.1, XM_006522907.2
UniGeneⁱ	Mm.18974

Genome annotation databases

Ensemblⁱ	ENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976 ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976
GeneIDⁱ	14390
KEGGⁱ	mmu:14390
UCSC genome browser More...UCSCⁱ	uc007ztj.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q00422	GA binding protein subunit alpha (Fragment)		MOUSE		186	UniRef100_Q00422
Gabpa protein		MOUSE		351

Protein	Similar proteins		Species	Score	Length	Source
Q00422	GA-binding protein alpha chain		HUMAN		454	UniRef90_Q06546
GA binding protein transcription factor alpha subunit		PANTR		454
GABPA isoform 1		PONAB		454
Uncharacterized protein		PANPA		454
Uncharacterized protein		NOMLE		454
+100

Protein	Similar proteins		Species	Score	Length	Source
Q00422	GA-binding protein alpha chain		HUMAN		454	UniRef50_Q06546
GA binding protein transcription factor alpha subunit		PANTR		454
GABPA isoform 1		PONAB		454
Uncharacterized protein		PANPA		454
Uncharacterized protein		NOMLE		454
+336

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M74515 mRNA Translation: AAA53030.1 AK139916 mRNA Translation: BAE24181.1 AK145446 mRNA Translation: BAE26442.1 AK145838 mRNA Translation: BAE26687.1 AK148087 mRNA Translation: BAE28337.1 AK150455 mRNA Translation: BAE29575.1 CH466521 Genomic DNA Translation: EDK98317.1 BC052448 mRNA Translation: AAH52448.1
CCDSⁱ	CCDS28284.1
PIRⁱ	A40858
RefSeqⁱ	NP_032091.2, NM_008065.2 XP_006522970.1, XM_006522907.2
UniGeneⁱ	Mm.18974

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1AWC	X-ray	2.15	A	320-429	[»]
	1SXD	NMR	-	A	168-254	[»]
	2JUO	NMR	-	A	35-121	[»]
ProteinModelPortalⁱ	Q00422
SMRⁱ	Q00422
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199795, 1 interactor
DIPⁱ	DIP-156N
IntActⁱ	Q00422, 1 interactor
MINTⁱ	Q00422
STRINGⁱ	10090.ENSMUSP00000009120

PTM databases

iPTMnetⁱ	Q00422
PhosphoSitePlusⁱ	Q00422

Proteomic databases

EPDⁱ	Q00422
MaxQBⁱ	Q00422
PaxDbⁱ	Q00422
PeptideAtlasⁱ	Q00422
PRIDEⁱ	Q00422

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976 ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976
GeneIDⁱ	14390
KEGGⁱ	mmu:14390
UCSCⁱ	uc007ztj.1 mouse

Organism-specific databases

CTDⁱ	2551
MGIⁱ	MGI:95610 Gabpa

Phylogenomic databases

eggNOGⁱ	KOG3806 Eukaryota ENOG410Z0ZF LUCA
GeneTreeⁱ	ENSGT00760000118907
HOGENOMⁱ	HOG000285952
HOVERGENⁱ	HBG051687
InParanoidⁱ	Q00422
KOⁱ	K09441
OMAⁱ	NVFWTHL
OrthoDBⁱ	EOG091G0EXE
TreeFamⁱ	TF350537

Enzyme and pathway databases

Reactomeⁱ	R-MMU-2151201 Transcriptional activation of mitochondrial biogenesis

Reactomeⁱ

R-MMU-2151201 Transcriptional activation of mitochondrial biogenesis

Miscellaneous databases

EvolutionaryTraceⁱ	Q00422
Protein Ontology More...PROⁱ	PR:Q00422
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000008976 Expressed in 322 organ(s), highest expression level in cleaving embryo
CleanExⁱ	MM_GABPA
Genevisibleⁱ	Q00422 MM

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR000418 Ets_dom IPR024668 GABP_asu_N IPR003118 Pointed_dom IPR013761 SAM/pointed_sf IPR016312 TF_GA-bd_asu IPR029071 Ubiquitin-like_domsf IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
Pfamⁱ	View protein in Pfam PF00178 Ets, 1 hit PF11620 GABP-alpha, 1 hit PF02198 SAM_PNT, 1 hit
PIRSFⁱ	PIRSF001703 GABP_alpha, 1 hit
PRINTSⁱ	PR00454 ETSDOMAIN
SMARTⁱ	View protein in SMART SM00413 ETS, 1 hit SM00251 SAM_PNT, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 1 hit SSF47769 SSF47769, 1 hit SSF54236 SSF54236, 1 hit
PROSITEⁱ	View protein in PROSITE PS00345 ETS_DOMAIN_1, 1 hit PS00346 ETS_DOMAIN_2, 1 hit PS50061 ETS_DOMAIN_3, 1 hit PS51433 PNT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GABPA_MOUSE
Accessionⁱ	Q00422Primary (citable) accession number: Q00422 Secondary accession number(s): Q7TT22
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
	Last sequence update:	July 27, 2011
	Last modified:	November 7, 2018
	This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniParc

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Supporting data

UniProtKB - Q00422 (GABPA_MOUSE)

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GA-binding protein alpha chain

Gabpa

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ