UniProtKB - Q00422 (GABPA_MOUSE)
Protein
GA-binding protein alpha chain
Gene
Gabpa
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor capable of interacting with purine rich repeats (GA repeats).
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 320 – 400 | ETSPROSITE-ProRule annotationAdd BLAST | 81 |
GO - Molecular functioni
- activating transcription factor binding Source: MGI
- chromatin binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- blastocyst formation Source: MGI
- cell differentiation Source: GO_Central
- cellular response to dopamine Source: MGI
- in utero embryonic development Source: MGI
- negative regulation of megakaryocyte differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-2151201, Transcriptional activation of mitochondrial biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: GA-binding protein alpha chainShort name: GABP subunit alpha |
Gene namesi | Name:Gabpa Synonyms:E4tf1a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95610, Gabpa |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204128 | 1 – 454 | GA-binding protein alpha chainAdd BLAST | 454 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 303 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q00422 |
MaxQBi | Q00422 |
PaxDbi | Q00422 |
PeptideAtlasi | Q00422 |
PRIDEi | Q00422 |
ProteomicsDBi | 273409 |
PTM databases
iPTMneti | Q00422 |
PhosphoSitePlusi | Q00422 |
Expressioni
Tissue specificityi
Ubiquitous.
Gene expression databases
Bgeei | ENSMUSG00000008976, Expressed in forelimb bud and 336 other tissues |
Genevisiblei | Q00422, MM |
Interactioni
Subunit structurei
Heterotetramer of two alpha and two beta subunits.
GO - Molecular functioni
- activating transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199795, 1 interactor |
DIPi | DIP-156N |
IntActi | Q00422, 1 interactor |
MINTi | Q00422 |
STRINGi | 10090.ENSMUSP00000009120 |
Miscellaneous databases
RNActi | Q00422, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q00422 |
SMRi | Q00422 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q00422 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 168 – 251 | PNTPROSITE-ProRule annotationAdd BLAST | 84 |
Sequence similaritiesi
Belongs to the ETS family.Curated
Phylogenomic databases
eggNOGi | KOG3806, Eukaryota |
GeneTreei | ENSGT00940000155799 |
HOGENOMi | CLU_037064_0_0_1 |
InParanoidi | Q00422 |
OMAi | QELEPHK |
OrthoDBi | 526256at2759 |
TreeFami | TF350537 |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR000418, Ets_dom IPR024668, GABP_asu_N IPR003118, Pointed_dom IPR013761, SAM/pointed_sf IPR016312, TF_GA-bd_asu IPR029071, Ubiquitin-like_domsf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00178, Ets, 1 hit PF11620, GABP-alpha, 1 hit PF02198, SAM_PNT, 1 hit |
PIRSFi | PIRSF001703, GABP_alpha, 1 hit |
PRINTSi | PR00454, ETSDOMAIN |
SMARTi | View protein in SMART SM00413, ETS, 1 hit SM00251, SAM_PNT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF47769, SSF47769, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00345, ETS_DOMAIN_1, 1 hit PS00346, ETS_DOMAIN_2, 1 hit PS50061, ETS_DOMAIN_3, 1 hit PS51433, PNT, 1 hit |
i Sequence
Sequence statusi: Complete.
Q00422-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYTPAECVSQ AIDINEPIGN
60 70 80 90 100
LKKLLEPRLQ CSLDAHEICL QDIQLDPDRS LFDQGVKTDG TVQLSVQVIS
110 120 130 140 150
YQGMEPKLNI LEIVKTAETV EVVIDPDAHH AEAEAHLVEE AQVITLDGTK
160 170 180 190 200
HITTISDETS EQVTRWAAAL EGYRKEQERL GIPYDPIHWS TDQVLHWVVW
210 220 230 240 250
VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL WSHLELLRKY
260 270 280 290 300
VLASQEQQMN EIVTIDQPVQ IIPASVPPAT PTTIKVINSS AKAAKVQRSP
310 320 330 340 350
RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL
360 370 380 390 400
NQPELVAQKW GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV
410 420 430 440 450
CDLKTLIGYS AAELNRLVIE CEQKKLARMQ LHGIAQPVTA VALAATSLQA
DKEI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 188 | H → R in AAA53030 (PubMed:1876836).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74515 mRNA Translation: AAA53030.1 AK139916 mRNA Translation: BAE24181.1 AK145446 mRNA Translation: BAE26442.1 AK145838 mRNA Translation: BAE26687.1 AK148087 mRNA Translation: BAE28337.1 AK150455 mRNA Translation: BAE29575.1 CH466521 Genomic DNA Translation: EDK98317.1 BC052448 mRNA Translation: AAH52448.1 |
CCDSi | CCDS28284.1 |
PIRi | A40858 |
RefSeqi | NP_032091.2, NM_008065.2 XP_006522970.1, XM_006522907.2 |
Genome annotation databases
Ensembli | ENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976 ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976 |
GeneIDi | 14390 |
KEGGi | mmu:14390 |
UCSCi | uc007ztj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M74515 mRNA Translation: AAA53030.1 AK139916 mRNA Translation: BAE24181.1 AK145446 mRNA Translation: BAE26442.1 AK145838 mRNA Translation: BAE26687.1 AK148087 mRNA Translation: BAE28337.1 AK150455 mRNA Translation: BAE29575.1 CH466521 Genomic DNA Translation: EDK98317.1 BC052448 mRNA Translation: AAH52448.1 |
CCDSi | CCDS28284.1 |
PIRi | A40858 |
RefSeqi | NP_032091.2, NM_008065.2 XP_006522970.1, XM_006522907.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AWC | X-ray | 2.15 | A | 320-429 | [»] | |
1SXD | NMR | - | A | 168-254 | [»] | |
2JUO | NMR | - | A | 35-121 | [»] | |
BMRBi | Q00422 | |||||
SMRi | Q00422 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199795, 1 interactor |
DIPi | DIP-156N |
IntActi | Q00422, 1 interactor |
MINTi | Q00422 |
STRINGi | 10090.ENSMUSP00000009120 |
PTM databases
iPTMneti | Q00422 |
PhosphoSitePlusi | Q00422 |
Proteomic databases
EPDi | Q00422 |
MaxQBi | Q00422 |
PaxDbi | Q00422 |
PeptideAtlasi | Q00422 |
PRIDEi | Q00422 |
ProteomicsDBi | 273409 |
Protocols and materials databases
Antibodypediai | 919, 403 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000009120; ENSMUSP00000009120; ENSMUSG00000008976 ENSMUST00000114184; ENSMUSP00000109822; ENSMUSG00000008976 |
GeneIDi | 14390 |
KEGGi | mmu:14390 |
UCSCi | uc007ztj.1, mouse |
Organism-specific databases
CTDi | 2551 |
MGIi | MGI:95610, Gabpa |
Phylogenomic databases
eggNOGi | KOG3806, Eukaryota |
GeneTreei | ENSGT00940000155799 |
HOGENOMi | CLU_037064_0_0_1 |
InParanoidi | Q00422 |
OMAi | QELEPHK |
OrthoDBi | 526256at2759 |
TreeFami | TF350537 |
Enzyme and pathway databases
Reactomei | R-MMU-2151201, Transcriptional activation of mitochondrial biogenesis |
Miscellaneous databases
BioGRID-ORCSi | 14390, 18 hits in 54 CRISPR screens |
ChiTaRSi | Gabpa, mouse |
EvolutionaryTracei | Q00422 |
PROi | PR:Q00422 |
RNActi | Q00422, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000008976, Expressed in forelimb bud and 336 other tissues |
Genevisiblei | Q00422, MM |
Family and domain databases
Gene3Di | 1.10.10.10, 1 hit 1.10.150.50, 1 hit |
InterProi | View protein in InterPro IPR000418, Ets_dom IPR024668, GABP_asu_N IPR003118, Pointed_dom IPR013761, SAM/pointed_sf IPR016312, TF_GA-bd_asu IPR029071, Ubiquitin-like_domsf IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF00178, Ets, 1 hit PF11620, GABP-alpha, 1 hit PF02198, SAM_PNT, 1 hit |
PIRSFi | PIRSF001703, GABP_alpha, 1 hit |
PRINTSi | PR00454, ETSDOMAIN |
SMARTi | View protein in SMART SM00413, ETS, 1 hit SM00251, SAM_PNT, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF47769, SSF47769, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00345, ETS_DOMAIN_1, 1 hit PS00346, ETS_DOMAIN_2, 1 hit PS50061, ETS_DOMAIN_3, 1 hit PS51433, PNT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GABPA_MOUSE | |
Accessioni | Q00422Primary (citable) accession number: Q00422 Secondary accession number(s): Q7TT22 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 189 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families