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Entry version 223 (29 Sep 2021)
Sequence version 1 (01 Dec 1992)
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Protein

Transcription initiation factor IIB

Gene

GTF2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682).

Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).

Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352).

Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171).

Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841).

Modulates transcription start site selection (PubMed:10318856).

Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).

19 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.117 mM for acetyl-CoA1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi15ZincPROSITE-ProRule annotationCombined sources2 Publications1
Metal bindingi18Zinc; via tele nitrogenPROSITE-ProRule annotationCombined sources2 Publications1
Metal bindingi34ZincPROSITE-ProRule annotationCombined sources2 Publications1
Metal bindingi37ZincPROSITE-ProRule annotationCombined sources2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri11 – 42TFIIB-typePROSITE-ProRule annotationAdd BLAST32

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionAcyltransferase, DNA-binding, Transferase
Biological processHost-virus interaction, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

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PathwayCommonsi
Q00403

Reactome - a knowledgebase of biological pathways and processes

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Reactomei
R-HSA-167161, HIV Transcription Initiation
R-HSA-167162, RNA Polymerase II HIV Promoter Escape
R-HSA-167172, Transcription of the HIV genome
R-HSA-674695, RNA Polymerase II Pre-transcription Events
R-HSA-6807505, RNA polymerase II transcribes snRNA genes
R-HSA-73776, RNA Polymerase II Promoter Escape
R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953, RNA Polymerase II Transcription Initiation
R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance

SIGNOR Signaling Network Open Resource

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SIGNORi
Q00403

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcription initiation factor IIB1 Publication (EC:2.3.1.481 Publication)
Alternative name(s):
General transcription factor TFIIB1 Publication
S300-II1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:GTF2B
Synonyms:TF2B, TFIIB
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:4648, GTF2B

Online Mendelian Inheritance in Man (OMIM)

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MIMi
189963, gene

neXtProt; the human protein knowledge platform

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neXtProti
NX_Q00403

Eukaryotic Pathogen, Vector and Host Database Resources

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VEuPathDBi
HostDB:ENSG00000137947

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi37C → S: Does not inhibit interaction with TBP. Inhibits the recruitment of RNA polymerase II into the initiation complex. 1 Publication1
Mutagenesisi51 – 56EWRTFS → AWRTFA: Partial loss of HIV-1 Vpr binding. 1 Publication6
Mutagenesisi51E → R, A or D: Defects in transcription start site selection. Supports a level of transcription equivalent to wild-type. 1 Publication1
Mutagenesisi52W → A: Partial loss of HIV-1 Vpr binding. 1 Publication1
Mutagenesisi53 – 54RT → AA: Partial loss of HIV-1 Vpr binding. 1 Publication2
Mutagenesisi55F → A: Partial loss of HIV-1 Vpr binding. 1 Publication1
Mutagenesisi66R → A, E or K: Defects in transcription start site selection. Supports a level of transcription equivalent to wild-type. 1 Publication1
Mutagenesisi153G → Q: Decreases BREd-dependent pre-initiation complex formation. 1 Publication1
Mutagenesisi185R → E: Reduces interaction with SSU72; when associated with E-193 or E-200. Inhibits interaction with VP16; when associated with E-193. Inhibits RNA pol II transcription activation induced by VP16 but does not affect basal transcription; when associated with E-193. 2 Publications1
Mutagenesisi185R → L: Reduces interaction with VP16; when associated with L-189. 1 Publication1
Mutagenesisi189K → E: Inhibits interaction with SSU72; when associated with E-193. Reduces interaction with SSU72; when associated with E-200. Inhibits interaction with VP16; when associated with E-200. Inhibits RNA pol II transcription activation induced by VP16 but does not affect basal transcription; when associated with E-200. 2 Publications1
Mutagenesisi189K → L: Reduces interaction with VP16; when associated with L-185. 1 Publication1
Mutagenesisi193R → E: Inhibits interaction with SSU72; when associated with E-185 or E-189. Inhibits interaction with VP16; when associated with E-185. Inhibits RNA pol II transcription activation induced by VP16 but does not affect basal transcription; when associated with E-185. 2 Publications1
Mutagenesisi196K → L: Reduces interaction with VP16; when associated with L-200. 1 Publication1
Mutagenesisi200 – 208KALETSVDL → GSGS: Reduces the formation of the TATA box-bound TBP ternary complex. 1 Publication9
Mutagenesisi200K → E: Reduces interaction with SSU72; when associated with E-185 or E-189. Inhibits interaction with VP16; when associated with E-189. Inhibits RNA pol II transcription activation induced by VP16 but does not affect basal transcription; when associated with E-189. 2 Publications1
Mutagenesisi200K → KGSGS: Reduces the formation of the TATA box-bound TBP ternary complex. 1 Publication1
Mutagenesisi200K → L: Reduces interaction with VP16; when associated with L-196. 1 Publication1
Mutagenesisi208L → LGSGS: Does not inhibit the formation of the TATA box-bound TBP ternary complex. 1 Publication1
Mutagenesisi238K → A: Abolishes autoacetylation, represses transcription activity, does not inhibit its association with chromatin to promoter-specific regions and decreases the association of GTF2F1 with chromatin to promoter-specific regions. 1 Publication1
Mutagenesisi247G → V: Inhibits interaction with TBP. 1 Publication1
Mutagenesisi283V → A: Reduces DNA-binding. 1 Publication1
Mutagenesisi286R → A: Reduces DNA-binding. 1 Publication1
Mutagenesisi286R → E: Inhibits interaction with RNA polymerase II; when associated with E-290 and E-295. 1 Publication1
Mutagenesisi290R → E: Inhibits interaction with RNA polymerase II; when associated with E-286 and E-295. 1 Publication1
Mutagenesisi295R → E: Inhibits interaction with RNA polymerase II; when associated with E-286 and E-290. 1 Publication1

Organism-specific databases

DisGeNET

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DisGeNETi
2959

Open Targets

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OpenTargetsi
ENSG00000137947

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA29035

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

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Pharosi
Q00403, Tbio

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
GTF2B

Domain mapping of disease mutations (DMDM)

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DMDMi
135629

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001192931 – 316Transcription initiation factor IIBAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei70PhosphoserineCombined sources1
Modified residuei76PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei238N6-acetyllysine; by autocatalysis1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Acetylated (PubMed:24441171). Autoacetylated; autoacetylation at Lys-238 stimulates transcription activation (PubMed:12931194).2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
Q00403

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
Q00403

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
Q00403

MaxQB - The MaxQuant DataBase

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MaxQBi
Q00403

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q00403

PeptideAtlas

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PeptideAtlasi
Q00403

PRoteomics IDEntifications database

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PRIDEi
Q00403

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
57846

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

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GlyGeni
Q00403, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
Q00403

MetOSite database of methionine sulfoxide sites

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MetOSitei
Q00403

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
Q00403

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in the inner cell mass forming the embryoblast (PubMed:24441171). Not detected in cells from the outer thin layer trophoblast (at protein level) (PubMed:24441171).1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000137947, Expressed in oocyte and 238 other tissues

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q00403, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q00403, HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
ENSG00000137947, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Found in a ternary complex with TATA box-bound TBP (PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:10619841, PubMed:29158257).

Part of a TFIID-containing RNA polymerase II pre-initiation complex (PIC) that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:27193682). Associates with TFIID-TFIIA (DA complex) to form TFIID-TFIIA-TFIIB (DAB complex), which is then recognized by RNA polymerase II (Pol II) (PubMed:1876184, PubMed:2247058).

Found in a RNA polymerase II initiation complex (PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8516312, PubMed:7601352).

Interacts (via C-terminus) with TBP; this interaction with TATA box-bound TBP guides Pol II into the PIC (PubMed:8504927, PubMed:10619841).

Interacts (via N-terminus) with Pol II (PubMed:8504927, PubMed:8413225).

Interacts (via C-terminus) with SSU72; this interaction is inhibited by SYMPK (PubMed:29158257).

Interacts with NR2F1; this interaction is direct (PubMed:1517211).

Interacts with PGR (PubMed:1517211).

Interacts with ESR1 (PubMed:1517211).

Interacts with GTF2F1 (via C-terminus and preferentially via acetylated form); this interaction prevents binding of GTF2B to GTF2F2 (PubMed:8662660, PubMed:12931194).

Interacts with GTF2F2 (via N-terminus); this interaction is inhibited in presence of GTF2F1 (PubMed:8504927, PubMed:8662660).

Interacts with the transcription elongation factor TCEA2 (PubMed:8566795).

Interacts with HSF1 (via transactivation domain) (PubMed:11005381).

Interacts with GPBP1 (By similarity).

By similarity18 Publications

(Microbial infection) Interacts with HIV-1 Vpr.

1 Publication

(Microbial infection) Interacts with Epstein-Barr virus EBNA2.

1 Publication

(Microbial infection) Interacts with Herpes simplex virus 1 protein ICP4.

1 Publication

(Microbial infection) Interacts (via C-terminus) with the Herpes simplex virus activator VP16; this interaction stimulates RNA Pol II transcription by increasing the extent of pre-initiation complex assembly.

2 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details
Q00403
With#Exp.IntAct
ANXA8 [P13928]3EBI-389564,EBI-2556915
APP [P05067]3EBI-389564,EBI-77613
ASCL4 [Q6XD76]3EBI-389564,EBI-10254793
BAHD1 [Q8TBE0]3EBI-389564,EBI-742750
C14orf93 - isoform 2 [Q9H972-2]3EBI-389564,EBI-11524174
CBLB [Q13191]3EBI-389564,EBI-744027
CBX2 - isoform 2 [Q14781-2]3EBI-389564,EBI-11974585
CSNK1D - isoform 2 [P48730-2]3EBI-389564,EBI-9087876
DCAF8 - isoform 2 [Q5TAQ9-2]3EBI-389564,EBI-25842815
DRAP1 [Q14919]6EBI-389564,EBI-712941
EP300 [Q09472]2EBI-389564,EBI-447295
FAM98C [Q17RN3]3EBI-389564,EBI-5461838
FAM9A [Q8IZU1]3EBI-389564,EBI-8468186
GOLGA6L9 [A6NEM1]3EBI-389564,EBI-5916454
HES4 [Q9HCC6]3EBI-389564,EBI-2680288
IK [Q13123]3EBI-389564,EBI-713456
JADE3 [Q92613]3EBI-389564,EBI-10278909
KLF15 [Q9UIH9]3EBI-389564,EBI-2796400
LPIN1 [Q14693]3EBI-389564,EBI-5278370
MLC1 [Q15049]3EBI-389564,EBI-8475277
MYC [P01106]3EBI-389564,EBI-447544
NVL - isoform 5 [O15381-5]3EBI-389564,EBI-18577082
PBX4 [Q9BYU1]3EBI-389564,EBI-10302990
PHF19 [Q5T6S3]3EBI-389564,EBI-2339674
POLR2E [P19388]5EBI-389564,EBI-395189
RNF183 [Q96D59]3EBI-389564,EBI-743938
RPP25 [Q9BUL9]3EBI-389564,EBI-366570
RPS4X [P62701]3EBI-389564,EBI-354303
RUSC1 [Q9BVN2]3EBI-389564,EBI-6257312
RYBP [Q8N488]3EBI-389564,EBI-752324
SAP30 [O75446]3EBI-389564,EBI-632609
SEM1 [P60896]3EBI-389564,EBI-79819
SPATA22 [Q8NHS9]3EBI-389564,EBI-7067260
SPPL2B - isoform 2 [Q8TCT7-2]3EBI-389564,EBI-8345366
SYNCRIP - isoform 4 [O60506-4]3EBI-389564,EBI-11123832
TBP [P20226]2EBI-389564,EBI-355371
TIMM44 [O43615]3EBI-389564,EBI-861737
TNIP1 [Q15025]7EBI-389564,EBI-357849
TRMT1L [Q7Z2T5]3EBI-389564,EBI-1237316
USP2 - isoform 3 [O75604-3]3EBI-389564,EBI-10696113
VSX2 [P58304]3EBI-389564,EBI-6427899
ZNF488 - isoform 2 [Q96MN9-2]3EBI-389564,EBI-25831733
ZNF829 - isoform 3 [Q3KNS6-3]3EBI-389564,EBI-18036029
ZSCAN1 - isoform 2 [Q8NBB4-2]3EBI-389564,EBI-12021938
ZSCAN22 [P10073]3EBI-389564,EBI-10178224
GRF4 [P46077] from Arabidopsis thaliana.2EBI-389564,EBI-637479
ICP0 [P87662] from Equid alphaherpesvirus 1.3EBI-389564,EBI-11712595
IE [P17473] from Equine herpesvirus 1 (strain Kentucky A).4EBI-389564,EBI-11702772
Vdr [P48281] from Mus musculus.2EBI-389564,EBI-346797

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
109214, 100 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
Q00403

Database of interacting proteins

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DIPi
DIP-1077N

Protein interaction database and analysis system

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IntActi
Q00403, 96 interactors

Molecular INTeraction database

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MINTi
Q00403

STRING: functional protein association networks

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STRINGi
9606.ENSP00000359531

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
Q00403, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q00403

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
Q00403

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati124 – 2001Add BLAST77
Repeati218 – 2942Add BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni189 – 193Core promoter DNA-binding1 Publication5
Regioni244 – 316Necessary for TATA box-bound TBP complex formation2 PublicationsAdd BLAST73
Regioni249 – 252Core promoter DNA-binding1 Publication4
Regioni283 – 286Core promoter DNA-binding2 Publications4

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The TFIIB-type zinc-binding domain is necessary for the interaction and recruitment of RNA polymerase II to the core promoter, the formation of a fully competent pre-initiation complex (PIC) assembly and basal transcription initiation (PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:8413225). The C-terminus is necessary and sufficient for interaction with the TATA box-bound TBP complex and for the formation of PIC (PubMed:8515820, PubMed:8516311, PubMed:8413225).4 Publications

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the TFIIB family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri11 – 42TFIIB-typePROSITE-ProRule annotationAdd BLAST32

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1597, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000006671

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q00403

Identification of Orthologs from Complete Genome Data

More...
OMAi
AFDHDQR

Database of Orthologous Groups

More...
OrthoDBi
729732at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q00403

TreeFam database of animal gene trees

More...
TreeFami
TF105953

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00043, CYCLIN, 2 hits

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...
IDEALi
IID00314

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013763, Cyclin-like
IPR036915, Cyclin-like_sf
IPR000812, TFIIB
IPR023486, TFIIB_CS
IPR013150, TFIIB_cyclin
IPR013137, Znf_TFIIB

The PANTHER Classification System

More...
PANTHERi
PTHR11618, PTHR11618, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF08271, TF_Zn_Ribbon, 1 hit
PF00382, TFIIB, 2 hits

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00685, TIFACTORIIB

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00385, CYCLIN, 2 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47954, SSF47954, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00782, TFIIB, 2 hits
PS51134, ZF_TFIIB, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q00403-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS
60 70 80 90 100
EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK
110 120 130 140 150
YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS
160 170 180 190 200
LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK
210 220 230 240 250
ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP
260 270 280 290 300
ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
310
PTDFKFDTPV DKLPQL
Length:316
Mass (Da):34,833
Last modified:December 1, 1992 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9CC7E102526C2722
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1APE2B1APE2_HUMAN
General transcription factor TFIIB
GTF2B
231Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
B1APE1B1APE1_HUMAN
General transcription factor TFIIB
GTF2B
224Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_01197719P → S. Corresponds to variant dbSNP:rs1804499Ensembl.1
Natural variantiVAR_035722132R → Q in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs144944840Ensembl.1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X59268 mRNA Translation: CAA41958.1
M76766 mRNA Translation: AAA61149.1
AK289822 mRNA Translation: BAF82511.1
AB451296 mRNA Translation: BAG70110.1
AL445991 Genomic DNA No translation available.
BC020597 mRNA Translation: AAH20597.1
S44184 mRNA Translation: AAB23144.1

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS715.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S17654, TWHU2B

NCBI Reference Sequences

More...
RefSeqi
NP_001505.1, NM_001514.5

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000370500; ENSP00000359531; ENSG00000137947

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
2959

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:2959

UCSC genome browser

More...
UCSCi
uc001dmo.5, human

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59268 mRNA Translation: CAA41958.1
M76766 mRNA Translation: AAA61149.1
AK289822 mRNA Translation: BAF82511.1
AB451296 mRNA Translation: BAG70110.1
AL445991 Genomic DNA No translation available.
BC020597 mRNA Translation: AAH20597.1
S44184 mRNA Translation: AAB23144.1
CCDSiCCDS715.1
PIRiS17654, TWHU2B
RefSeqiNP_001505.1, NM_001514.5

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C9BX-ray2.65A/E/I/M/Q110-316[»]
1DL6NMR-A2-59[»]
1RLYNMR-A1-60[»]
1RO4NMR-A1-60[»]
1TFBNMR-A112-316[»]
1VOLX-ray2.70A113-316[»]
2PHGNMR-A112-316[»]
5IY6electron microscopy7.20M1-316[»]
5IY7electron microscopy8.60M1-316[»]
5IY8electron microscopy7.90M1-316[»]
5IY9electron microscopy6.30M1-316[»]
5IYAelectron microscopy5.40M1-316[»]
5IYBelectron microscopy3.90M1-316[»]
5IYCelectron microscopy3.90M1-316[»]
5IYDelectron microscopy3.90M1-316[»]
5WH1X-ray3.39A/B/C/D107-316[»]
6O9Lelectron microscopy7.20M1-316[»]
7EDXelectron microscopy4.50R1-316[»]
7EG7electron microscopy6.20R1-316[»]
7EG8electron microscopy7.40R1-316[»]
7EG9electron microscopy3.70R1-316[»]
7EGAelectron microscopy4.10R1-316[»]
7EGBelectron microscopy3.30R1-316[»]
7EGCelectron microscopy3.90R1-316[»]
7ENAelectron microscopy4.07BA1-316[»]
7ENCelectron microscopy4.13BA1-316[»]
7LBMelectron microscopy4.80O1-316[»]
7NVRelectron microscopy4.50M1-316[»]
7NVSelectron microscopy2.80M1-316[»]
7NVTelectron microscopy2.90M1-316[»]
7NVUelectron microscopy2.50M1-316[»]
7NVYelectron microscopy7.30M1-316[»]
7NVZelectron microscopy7.20M1-316[»]
7NW0electron microscopy6.60M1-316[»]
SMRiQ00403
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi109214, 100 interactors
CORUMiQ00403
DIPiDIP-1077N
IntActiQ00403, 96 interactors
MINTiQ00403
STRINGi9606.ENSP00000359531

PTM databases

GlyGeniQ00403, 1 site, 1 O-linked glycan (1 site)
iPTMnetiQ00403
MetOSiteiQ00403
PhosphoSitePlusiQ00403

Genetic variation databases

BioMutaiGTF2B
DMDMi135629

Proteomic databases

EPDiQ00403
jPOSTiQ00403
MassIVEiQ00403
MaxQBiQ00403
PaxDbiQ00403
PeptideAtlasiQ00403
PRIDEiQ00403
ProteomicsDBi57846

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...
Antibodypediai
19819, 442 antibodies

The DNASU plasmid repository

More...
DNASUi
2959

Genome annotation databases

EnsembliENST00000370500; ENSP00000359531; ENSG00000137947
GeneIDi2959
KEGGihsa:2959
UCSCiuc001dmo.5, human

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
2959
DisGeNETi2959

GeneCards: human genes, protein and diseases

More...
GeneCardsi
GTF2B
HGNCiHGNC:4648, GTF2B
HPAiENSG00000137947, Low tissue specificity
MIMi189963, gene
neXtProtiNX_Q00403
OpenTargetsiENSG00000137947
PharmGKBiPA29035
VEuPathDBiHostDB:ENSG00000137947

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1597, Eukaryota
GeneTreeiENSGT00390000006671
InParanoidiQ00403
OMAiAFDHDQR
OrthoDBi729732at2759
PhylomeDBiQ00403
TreeFamiTF105953

Enzyme and pathway databases

PathwayCommonsiQ00403
ReactomeiR-HSA-167161, HIV Transcription Initiation
R-HSA-167162, RNA Polymerase II HIV Promoter Escape
R-HSA-167172, Transcription of the HIV genome
R-HSA-674695, RNA Polymerase II Pre-transcription Events
R-HSA-6807505, RNA polymerase II transcribes snRNA genes
R-HSA-73776, RNA Polymerase II Promoter Escape
R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953, RNA Polymerase II Transcription Initiation
R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance
SIGNORiQ00403

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
2959, 804 hits in 1031 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
GTF2B, human
EvolutionaryTraceiQ00403

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
Transcription_factor_II_B

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
2959
PharosiQ00403, Tbio

Protein Ontology

More...
PROi
PR:Q00403
RNActiQ00403, protein

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000137947, Expressed in oocyte and 238 other tissues
ExpressionAtlasiQ00403, baseline and differential
GenevisibleiQ00403, HS

Family and domain databases

CDDicd00043, CYCLIN, 2 hits
IDEALiIID00314
InterProiView protein in InterPro
IPR013763, Cyclin-like
IPR036915, Cyclin-like_sf
IPR000812, TFIIB
IPR023486, TFIIB_CS
IPR013150, TFIIB_cyclin
IPR013137, Znf_TFIIB
PANTHERiPTHR11618, PTHR11618, 1 hit
PfamiView protein in Pfam
PF08271, TF_Zn_Ribbon, 1 hit
PF00382, TFIIB, 2 hits
PRINTSiPR00685, TIFACTORIIB
SMARTiView protein in SMART
SM00385, CYCLIN, 2 hits
SUPFAMiSSF47954, SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00782, TFIIB, 2 hits
PS51134, ZF_TFIIB, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTF2B_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q00403
Secondary accession number(s): A8K1A7, Q5JS30
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 29, 2021
This is version 223 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families
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