UniProtKB - Q00284 (Q00284_ASPPA)
Protein
Nitrate reductase
Gene
niaD
Organism
Aspergillus parasiticus
Status
Functioni
Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.UniRule annotationARBA annotation
Catalytic activityi
- EC:1.7.1.3ARBA annotation
Cofactori
Protein has several cofactor binding sites:- FADARBA annotation
- Mo-molybdopterinUniRule annotationNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.UniRule annotation
- hemeARBA annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 152 | MolybdenumUniRule annotation | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- molybdenum ion binding Source: InterPro
- molybdopterin cofactor binding Source: InterPro
- nitrate reductase (NADPH) activity Source: UniProtKB-EC
GO - Biological processi
- nitrate assimilation Source: UniProtKB-KW
- nitric oxide biosynthetic process Source: InterPro
Keywordsi
Molecular function | OxidoreductaseARBA annotation |
Biological process | Nitrate assimilationUniRule annotationARBA annotation |
Ligand | FADARBA annotation, Flavoprotein, HemeARBA annotation, Iron, Metal-binding, MolybdenumUniRule annotationARBA annotation, NADPARBA annotation |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrate reductaseUniRule annotation |
Gene namesi | Name:niaDImported |
Organismi | Aspergillus parasiticusImported |
Taxonomic identifieri | 5067 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus |
Interactioni
Subunit structurei
Homodimer.
ARBA annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 514 – 589 | Cytochrome b5 heme-bindingInterPro annotationAdd BLAST | 76 | |
Domaini | 615 – 735 | FAD-binding FR-typeInterPro annotationAdd BLAST | 121 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 35 – 67 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 479 – 510 | DisorderedSequence analysisAdd BLAST | 32 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 488 – 510 | PolyampholyteSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit 3.40.50.80, 1 hit 3.90.420.10, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR014756, Ig_E-set IPR005066, MoCF_OxRdtse_dimer IPR008335, Mopterin_OxRdtase_euk IPR012137, Nitr_rd_NADH IPR001433, OxRdtase_FAD/NAD-bd IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR022407, OxRdtase_Mopterin_BS IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00970, FAD_binding_6, 1 hit PF03404, Mo-co_dimer, 1 hit PF00175, NAD_binding_1, 1 hit PF00174, Oxidored_molyb, 1 hit |
PIRSFi | PIRSF000233, Nitr_rd_NADH, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00363, CYTOCHROMEB5 PR00407, EUMOPTERIN PR00371, FPNCR |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF55856, SSF55856, 1 hit SSF56524, SSF56524, 1 hit SSF63380, SSF63380, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS51384, FAD_FR, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
i Sequence
Sequence statusi: Complete.
Q00284-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATITEVRTD ALVPTDLVLK TGQIKIQSEE ISTKDLSDIP LPPPSKRPTE
60 70 80 90 100
VLSVDKGTPD SHVPRDPRLI RLTGVHPFNV EPPLTDLYKE GFLTSPELFY
110 120 130 140 150
VRNHGPVPHV KDEDIPHWEI TIEGLVEKPL VLNFRQVLQQ YDQITAPITL
160 170 180 190 200
VCAGNRRKEQ NIVRKTKGFS WGSAGLSTAL FTGPLLADIL RSGKPLRQAK
210 220 230 240 250
YVCMEGADKL PNGHYGTLIK LNWALDPNRG IMLAHKMNGE SLRPDHGRPL
260 270 280 290 300
RAVVPGQIGG RSVKWLKRLI LTDAPSDNWY HINDNRVLPT MVSPDMASNN
310 320 330 340 350
RNWWHDERDA IYDLNTNSAV GYPQNNEVLN ILEARAVIYC QRIAYAGGGR
360 370 380 390 400
RVTRVEISLD KGKSWRLADI EYAEDKYRDF EGELFGGKVD MYWRETCFCW
410 420 430 440 450
CFWSLSIAIP ELENSDAILV RAMDEALGVQ PRDMYWSVLG MMNNPWFRVT
460 470 480 490 500
ITKENGNLRF EHPTHPSMPT GWMERVKKAG GDPTNGNWGE RHEGEEPTEP
510 520 530 540 550
EPVQDINMKK DGPSRTISFE EFKENSCDEK PWFIVNGEVY DGQAFLEGHP
560 570 580 590 600
GGRQSIISSA GQDVSEEFLA IHSETAKAMM PEYHIGTTDP EGLIALKDDA
610 620 630 640 650
SSSTDEIRPV FLQSRSWTKA TLKERKDISW DTRIFSFKLE HEDQTLGLPV
660 670 680 690 700
GQHLMIKVLD RSSNNEAIIR SYTPISETSQ KGTVDLLVKV YFATATSAGG
710 720 730 740 750
KMTMALDRLP LGSVVEYLGN GRVLISGKER HVRSFKMICG GTGITPILQV
760 770 780 790 800
LRAVVQDHQD PTSCVVLNGN RQEEDILRRA ELDGFMASDS RRCNIIHTLS
810 820 830 840 850
KAPDSWTGRR GRISEELLKE YAAPEDESMV LICGPPAMEE SARRILLAEG
WKESDLHFF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38948 Genomic DNA Translation: AAC49605.1 |
PIRi | S70584 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U38948 Genomic DNA Translation: AAC49605.1 |
PIRi | S70584 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit 3.40.50.80, 1 hit 3.90.420.10, 1 hit |
InterProi | View protein in InterPro IPR001834, CBR-like IPR008333, Cbr1-like_FAD-bd_dom IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR039261, FNR_nucleotide-bd IPR014756, Ig_E-set IPR005066, MoCF_OxRdtse_dimer IPR008335, Mopterin_OxRdtase_euk IPR012137, Nitr_rd_NADH IPR001433, OxRdtase_FAD/NAD-bd IPR000572, OxRdtase_Mopterin-bd_dom IPR036374, OxRdtase_Mopterin-bd_sf IPR022407, OxRdtase_Mopterin_BS IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00970, FAD_binding_6, 1 hit PF03404, Mo-co_dimer, 1 hit PF00175, NAD_binding_1, 1 hit PF00174, Oxidored_molyb, 1 hit |
PIRSFi | PIRSF000233, Nitr_rd_NADH, 1 hit |
PRINTSi | PR00406, CYTB5RDTASE PR00363, CYTOCHROMEB5 PR00407, EUMOPTERIN PR00371, FPNCR |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF55856, SSF55856, 1 hit SSF56524, SSF56524, 1 hit SSF63380, SSF63380, 1 hit SSF81296, SSF81296, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit PS51384, FAD_FR, 1 hit PS00559, MOLYBDOPTERIN_EUK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q00284_ASPPA | |
Accessioni | Q00284Primary (citable) accession number: Q00284 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | November 1, 1996 |
Last sequence update: | November 1, 1996 | |
Last modified: | October 7, 2020 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |