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Protein

Protein TraI

Gene

traI

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The initiation process of transfer DNA synthesis requires the interaction of at least three plasmid-specific components (TraH, I, and J) at the transfer origin resulting in the assembly of a specialised nucleoprotein complex - the relaxosome. Site- and strand-specific cleavage at the transfer origin is dependent on TraI and TraJ.

GO - Biological processi

Keywordsi

Biological processConjugation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TraI
Gene namesi
Name:traI
Encoded oniPlasmid IncP-alpha RP40 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000684551 – 732Protein TraIAdd BLAST732

Proteomic databases

PRIDEiQ00191

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR005094 Endonuclease_MobA/VirD2
PfamiView protein in Pfam
PF03432 Relaxase, 1 hit

Sequencei

Sequence statusi: Complete.

Q00191-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIAKHVPMRS IKKSDFAELV KYITDEQGKT ERLGHVRVTN CEANTLPAVM
60 70 80 90 100
AEVMATQHGN TRSEADKTYH LLVSFRAGEK PDAETLRAIE DRICAGLGFA
110 120 130 140 150
EHQRVSAVHH DTDNLHIHIA INKIHPTRNT IHEPYRAYRA LADLCATLER
160 170 180 190 200
DYGLERDNHE TRQRVSENRA NDMERHAGVE SLVGWIKREC LPELQAAQSW
210 220 230 240 250
EDLHRVLREN GLKLRERGNG FIFEAGDGTT VKASTVSRDL SKPKLEARFG
260 270 280 290 300
AFTPAEGGEA PRRREYRAKP LKTRIDTTEL YARYQSERQE MGAVRKGELD
310 320 330 340 350
TLRRRRDRLI EAAMRSNRLR RAAIKLLGEG RIAKRLMYAQ AHKALRADLD
360 370 380 390 400
KINREYRQGR QAVQERTQRR AWADWLKAEA MKGDDKALAA LRAREGRSDL
410 420 430 440 450
KGNTIQGSGE AKPGHAAVTD NITKKGTIIY RVGSSAVRDD GDRLQVSREA
460 470 480 490 500
TTDGLDAALR LAMERFGDRI TVNGTAEFKE RIAQAAAAGR LAITFDDAAL
510 520 530 540 550
ERRRQELLTK EQAHEQPERN DGRRDRGGDG GIRPAAARTT LNATGGDGDR
560 570 580 590 600
RDARAVSAGG TVALRKPNVG RIGRKPPPQS QNRLRALSQL GVVRIAGGAE
610 620 630 640 650
MLLPRDVPGH VEQQGAEPAH ALRRGVSGPG RGLKPEQIAA AEKYVAEREQ
660 670 680 690 700
KRLNGFDIPK HARYTDYVGA LSYAGTRNVE DQALALLRKE NDEILVLPVD
710 720 730
KATVQRMKRL AIGDPVTVTP RGSLKTTRGR SR
Length:732
Mass (Da):81,563
Last modified:February 1, 1994 - v1
Checksum:iBD97EA7DB14E96AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54459 Genomic DNA Translation: CAA38336.1
PIRiS23001
RefSeqiWP_011205818.1, NZ_NJTR01000055.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54459 Genomic DNA Translation: CAA38336.1
PIRiS23001
RefSeqiWP_011205818.1, NZ_NJTR01000055.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ00191

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR005094 Endonuclease_MobA/VirD2
PfamiView protein in Pfam
PF03432 Relaxase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRAI6_ECOLX
AccessioniPrimary (citable) accession number: Q00191
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 10, 2018
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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