UniProtKB - Q00169 (PIPNA_HUMAN)
Protein
Phosphatidylinositol transfer protein alpha isoform
Gene
PITPNA
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed:10531358, PubMed:22822086, PubMed:15522822, PubMed:18636990, PubMed:14962392). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (PubMed:15522822, PubMed:22822086). Preference order for PC is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 and for PI is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 > C20:3 (PubMed:22822086).5 Publications
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)3 PublicationsThis reaction proceeds in the forward1 Publication direction.
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)5 PublicationsThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Phosphatidylinositol transfer activity is inhibited by N-ethylmaleimide.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 58 | Phosphatidylinositol1 Publication | 1 | |
Binding sitei | 60 | Phosphatidylinositol1 Publication | 1 | |
Binding sitei | 85 | Phosphatidylinositol1 Publication | 1 | |
Binding sitei | 89 | Phosphatidylinositol1 Publication | 1 | |
Binding sitei | 96 | Phosphatidylinositol1 Publication | 1 | |
Binding sitei | 194 | Phosphatidylinositol1 Publication | 1 |
GO - Molecular functioni
- phosphatidylcholine binding Source: UniProtKB
- phosphatidylcholine transfer activity Source: UniProtKB
- phosphatidylcholine transporter activity Source: BHF-UCL
- phosphatidylglycerol binding Source: BHF-UCL
- phosphatidylinositol binding Source: UniProtKB
- phosphatidylinositol transfer activity Source: UniProtKB
GO - Biological processi
- interleukin-12-mediated signaling pathway Source: Reactome
- lipid metabolic process Source: ProtInc
- phospholipid transport Source: BHF-UCL
- visual perception Source: ProtInc
Keywordsi
Biological process | Transport |
Ligand | Lipid-binding |
Enzyme and pathway databases
PathwayCommonsi | Q00169 |
Reactomei | R-HSA-418890, Role of second messengers in netrin-1 signaling R-HSA-8950505, Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation |
Chemistry databases
SwissLipidsi | SLP:000000415 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol transfer protein alpha isoformShort name: PI-TP-alpha Short name: PtdIns transfer protein alpha Short name: PtdInsTP alpha |
Gene namesi | Name:PITPNA Synonyms:PITPN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9001, PITPNA |
MIMi | 600174, gene |
neXtProti | NX_Q00169 |
VEuPathDBi | HostDB:ENSG00000174238.14 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 58 | T → A: Reduced phosphatidylinositol and phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 58 | T → E: Complete loss of phosphatidylinositol transfer activity but no effect on phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 58 | T → S: Reduced phosphatidylinositol transfer activity but no effect on phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 60 | K → A: Complete loss of phosphatidylinositol transfer activity but no effect on phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 85 | E → A: Reduced phosphatidylinositol transfer activity but no effect on phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 85 | E → Q: Reduced phosphatidylinositol and phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 89 | N → F or L: Significant loss of phosphatidylinositol transfer activity but no effect on phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 94 | C → A or T: No effect on phosphatidylinositol transfer activity. Resistant to inhibition by N-ethylmaleimide. 1 Publication | 1 | |
Mutagenesisi | 102 | Y → A: Reduced phosphatidylinositol and phosphatidylcholine transfer activity. 1 Publication | 1 | |
Mutagenesisi | 187 | C → A: No effect on phosphatidylinositol transfer activity. 1 Publication | 1 | |
Mutagenesisi | 202 – 203 | WW → AA: Significant loss of phosphatidylinositol and phosphatidylcholine transfer activity. 2 Publications | 2 |
Organism-specific databases
DisGeNETi | 5306 |
OpenTargetsi | ENSG00000174238 |
PharmGKBi | PA33335 |
Miscellaneous databases
Pharosi | Q00169, Tbio |
Chemistry databases
DrugBanki | DB03690, (Z,Z)-4-Hydroxy-N,N,N-Trimethyl-10-Oxo-7-[(1-Oxo-9-Octadecenyl)Oxy]-3,5,9-Trioxa-4-Phosphaheptacos-18-En-1-Aminium-4-Oxide DB02144, 1,2-diacyl-sn-glycero-3-phosphoinositol |
Genetic variation databases
BioMutai | PITPNA |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000191639 | 2 – 270 | Phosphatidylinositol transfer protein alpha isoformAdd BLAST | 269 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 215 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q00169 |
jPOSTi | Q00169 |
MassIVEi | Q00169 |
PaxDbi | Q00169 |
PeptideAtlasi | Q00169 |
PRIDEi | Q00169 |
ProteomicsDBi | 57841 |
PTM databases
iPTMneti | Q00169 |
MetOSitei | Q00169 |
PhosphoSitePlusi | Q00169 |
Expressioni
Gene expression databases
Bgeei | ENSG00000174238, Expressed in pigmented layer of retina and 246 other tissues |
ExpressionAtlasi | Q00169, baseline and differential |
Genevisiblei | Q00169, HS |
Organism-specific databases
HPAi | ENSG00000174238, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsQ00169
With | #Exp. | IntAct |
---|---|---|
CASP6 [P55212] | 3 | EBI-1042490,EBI-718729 |
LAMP2 - isoform LAMP-2B [P13473-2] | 3 | EBI-1042490,EBI-21591415 |
SH3GLB1 [Q9Y371] | 3 | EBI-1042490,EBI-2623095 |
UQCRC1 [P31930] | 3 | EBI-1042490,EBI-1052596 |
Protein-protein interaction databases
BioGRIDi | 111323, 27 interactors |
IntActi | Q00169, 13 interactors |
MINTi | Q00169 |
STRINGi | 9606.ENSP00000316809 |
Miscellaneous databases
RNActi | Q00169, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q00169 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q00169 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3668, Eukaryota |
GeneTreei | ENSGT00940000157119 |
HOGENOMi | CLU_046509_0_0_1 |
InParanoidi | Q00169 |
OMAi | RTAFQNE |
OrthoDBi | 951268at2759 |
PhylomeDBi | Q00169 |
TreeFami | TF313279 |
Family and domain databases
DisProti | DP02327 |
Gene3Di | 3.30.530.20, 1 hit |
InterProi | View protein in InterPro IPR001666, PI_transfer IPR023393, START-like_dom_sf |
PANTHERi | PTHR10658, PTHR10658, 1 hit |
Pfami | View protein in Pfam PF02121, IP_trans, 1 hit |
PRINTSi | PR00391, PITRANSFER |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All
Q00169-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLLKEYRVI LPVSVDEYQV GQLYSVAEAS KNETGGGEGV EVLVNEPYEK
60 70 80 90 100
DGEKGQYTHK IYHLQSKVPT FVRMLAPEGA LNIHEKAWNA YPYCRTVITN
110 120 130 140 150
EYMKEDFLIK IETWHKPDLG TQENVHKLEP EAWKHVEAVY IDIADRSQVL
160 170 180 190 200
SKDYKAEEDP AKFKSIKTGR GPLGPNWKQE LVNQKDCPYM CAYKLVTVKF
210 220 230 240 250
KWWGLQNKVE NFIHKQERRL FTNFHRQLFC WLDKWVDLTM DDIRRMEEET
260 270
KRQLDEMRQK DPVKGMTADD
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L4U7 | I3L4U7_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 121 | Annotation score: | ||
F5GWE5 | F5GWE5_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 270 | Annotation score: | ||
I3L4C0 | I3L4C0_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 115 | Annotation score: | ||
I3L2X8 | I3L2X8_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 83 | Annotation score: | ||
I3L4H1 | I3L4H1_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 209 | Annotation score: | ||
I3L3W1 | I3L3W1_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 44 | Annotation score: | ||
I3NI10 | I3NI10_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 66 | Annotation score: | ||
I3L459 | I3L459_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 142 | Annotation score: | ||
I3L471 | I3L471_HUMAN | Phosphatidylinositol transfer prote... | PITPNA | 98 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 45 | N → P in BAA06276 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73704 mRNA Translation: AAA36441.1 D30036 mRNA Translation: BAA06276.1 BC082976 mRNA Translation: AAH82976.1 BC045108 mRNA Translation: AAH45108.1 |
CCDSi | CCDS45563.1 |
PIRi | I53775 |
RefSeqi | NP_006215.1, NM_006224.3 |
Genome annotation databases
Ensembli | ENST00000313486; ENSP00000316809; ENSG00000174238 |
GeneIDi | 5306 |
KEGGi | hsa:5306 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M73704 mRNA Translation: AAA36441.1 D30036 mRNA Translation: BAA06276.1 BC082976 mRNA Translation: AAH82976.1 BC045108 mRNA Translation: AAH45108.1 |
CCDSi | CCDS45563.1 |
PIRi | I53775 |
RefSeqi | NP_006215.1, NM_006224.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1UW5 | X-ray | 2.90 | A/B/C/D | 1-270 | [»] | |
SMRi | Q00169 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111323, 27 interactors |
IntActi | Q00169, 13 interactors |
MINTi | Q00169 |
STRINGi | 9606.ENSP00000316809 |
Chemistry databases
DrugBanki | DB03690, (Z,Z)-4-Hydroxy-N,N,N-Trimethyl-10-Oxo-7-[(1-Oxo-9-Octadecenyl)Oxy]-3,5,9-Trioxa-4-Phosphaheptacos-18-En-1-Aminium-4-Oxide DB02144, 1,2-diacyl-sn-glycero-3-phosphoinositol |
SwissLipidsi | SLP:000000415 |
PTM databases
iPTMneti | Q00169 |
MetOSitei | Q00169 |
PhosphoSitePlusi | Q00169 |
Genetic variation databases
BioMutai | PITPNA |
Proteomic databases
EPDi | Q00169 |
jPOSTi | Q00169 |
MassIVEi | Q00169 |
PaxDbi | Q00169 |
PeptideAtlasi | Q00169 |
PRIDEi | Q00169 |
ProteomicsDBi | 57841 |
Protocols and materials databases
Antibodypediai | 5311, 183 antibodies |
DNASUi | 5306 |
Genome annotation databases
Ensembli | ENST00000313486; ENSP00000316809; ENSG00000174238 |
GeneIDi | 5306 |
KEGGi | hsa:5306 |
Organism-specific databases
CTDi | 5306 |
DisGeNETi | 5306 |
GeneCardsi | PITPNA |
HGNCi | HGNC:9001, PITPNA |
HPAi | ENSG00000174238, Low tissue specificity |
MIMi | 600174, gene |
neXtProti | NX_Q00169 |
OpenTargetsi | ENSG00000174238 |
PharmGKBi | PA33335 |
VEuPathDBi | HostDB:ENSG00000174238.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3668, Eukaryota |
GeneTreei | ENSGT00940000157119 |
HOGENOMi | CLU_046509_0_0_1 |
InParanoidi | Q00169 |
OMAi | RTAFQNE |
OrthoDBi | 951268at2759 |
PhylomeDBi | Q00169 |
TreeFami | TF313279 |
Enzyme and pathway databases
PathwayCommonsi | Q00169 |
Reactomei | R-HSA-418890, Role of second messengers in netrin-1 signaling R-HSA-8950505, Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation |
Miscellaneous databases
BioGRID-ORCSi | 5306, 7 hits in 995 CRISPR screens |
ChiTaRSi | PITPNA, human |
EvolutionaryTracei | Q00169 |
GeneWikii | Phosphatidylinositol_transfer_protein,_alpha |
GenomeRNAii | 5306 |
Pharosi | Q00169, Tbio |
PROi | PR:Q00169 |
RNActi | Q00169, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000174238, Expressed in pigmented layer of retina and 246 other tissues |
ExpressionAtlasi | Q00169, baseline and differential |
Genevisiblei | Q00169, HS |
Family and domain databases
DisProti | DP02327 |
Gene3Di | 3.30.530.20, 1 hit |
InterProi | View protein in InterPro IPR001666, PI_transfer IPR023393, START-like_dom_sf |
PANTHERi | PTHR10658, PTHR10658, 1 hit |
Pfami | View protein in Pfam PF02121, IP_trans, 1 hit |
PRINTSi | PR00391, PITRANSFER |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIPNA_HUMAN | |
Accessioni | Q00169Primary (citable) accession number: Q00169 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 17
Human chromosome 17: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families