UniProtKB - Q00168 (KCC2A_DROME)
Calcium/calmodulin-dependent protein kinase type II alpha chain
CaMKII
Functioni
A key regulator of plasticity in synaptic physiology and behavior, alterations in its activity produce pleiotrophic effects that involve synaptic transmission and development as well as various aspects of behavior. Directly modulates eag potassium channels.
2 PublicationsCatalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 43 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 136 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 28 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein kinase activity Source: FlyBase
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: FlyBase
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cellular response to starvation Source: FlyBase
- chemical synaptic transmission Source: FlyBase
- courtship behavior Source: FlyBase
- learning or memory Source: FlyBase
- long-term memory Source: FlyBase
- male courtship behavior Source: FlyBase
- negative regulation of cytokine production Source: FlyBase
- negative regulation of lipid storage Source: FlyBase
- neuromuscular junction development Source: FlyBase
- peptidyl-threonine autophosphorylation Source: FlyBase
- positive regulation of synaptic transmission, dopaminergic Source: UniProtKB
- protein phosphorylation Source: FlyBase
- regulation of filopodium assembly Source: FlyBase
- regulation of ovulation Source: FlyBase
- regulation of synaptic assembly at neuromuscular junction Source: FlyBase
Keywordsi
Molecular function | Calmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.17, 1994 |
Reactomei | R-DME-3371571, HSF1-dependent transactivation R-DME-4086398, Ca2+ pathway R-DME-438066, Unblocking of NMDA receptors, glutamate binding and activation R-DME-5578775, Ion homeostasis R-DME-5673000, RAF activation R-DME-936837, Ion transport by P-type ATPases |
SignaLinki | Q00168 |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium/calmodulin-dependent protein kinase type II alpha chain (EC:2.7.11.17)Short name: CaM-kinase II alpha chain |
Gene namesi | Name:CaMKII Synonyms:CaM ORF Names:CG18069 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0264607, CaMKII |
VEuPathDBi | VectorBase:FBgn0264607 |
Subcellular locationi
Plasma Membrane
- postsynaptic membrane Source: FlyBase
Other locations
- axon Source: FlyBase
- cytoplasm Source: FlyBase
- dendrite Source: FlyBase
- neuron projection Source: GO_Central
- presynaptic active zone Source: FlyBase
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 306 | T → A: Fails to interact with CASK, catalytically active but fails to autophosphorylate, when associated with A-307. 1 Publication | 1 | |
Mutagenesisi | 306 | T → D: Fails to interact with CASK, kinase inactive, when associated with D-307. 1 Publication | 1 | |
Mutagenesisi | 306 | T → S: Fails to interact with CASK, catalytically active and can autophosphorylate, when associated with S-307. 1 Publication | 1 | |
Mutagenesisi | 307 | T → A: Fails to interact with CASK, catalytically active but fails to autophosphorylate, when associated with A-306. 1 Publication | 1 | |
Mutagenesisi | 307 | T → D: Fails to interact with CASK, kinase inactive, when associated with D-306. 1 Publication | 1 | |
Mutagenesisi | 307 | T → S: Fails to interact with CASK, catalytically active and can autophosphorylate, when associated with S-306. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086095 | 1 – 530 | Calcium/calmodulin-dependent protein kinase type II alpha chainAdd BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 287 | Phosphothreonine; by autocatalysis2 Publications | 1 | |
Modified residuei | 306 | Phosphothreonine; by autocatalysis1 Publication | 1 | |
Modified residuei | 307 | Phosphothreonine; by autocatalysis1 Publication | 1 | |
Modified residuei | 327 | Phosphoserine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q00168 |
PTM databases
iPTMneti | Q00168 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | FBgn0264607, Expressed in head and 55 other tissues |
ExpressionAtlasi | Q00168, baseline and differential |
Genevisiblei | Q00168, DM |
Interactioni
Subunit structurei
Interacts with CASK.
1 PublicationBinary interactionsi
Q00168
With | #Exp. | IntAct |
---|---|---|
CASK [Q24210] | 5 | EBI-124595,EBI-214423 |
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 68654, 31 interactors |
IntActi | Q00168, 5 interactors |
MINTi | Q00168 |
STRINGi | 7227.FBpp0289608 |
Structurei
Secondary structure
3D structure databases
SMRi | Q00168 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 272 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 261 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 291 – 301 | Calmodulin-bindingAdd BLAST | 11 | |
Regioni | 320 – 358 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 320 – 335 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 336 – 358 | Basic and acidic residuesSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0033, Eukaryota |
GeneTreei | ENSGT00940000159769 |
InParanoidi | Q00168 |
PhylomeDBi | Q00168 |
Family and domain databases
InterProi | View protein in InterPro IPR013543, Ca/CaM-dep_prot_kinase-assoc IPR011009, Kinase-like_dom_sf IPR032710, NTF2-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF08332, CaMKII_AD, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF54427, SSF54427, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAPAACTRF SDNYDIKEEL GKGAFSIVKR CVQKSTGFEF AAKIINTKKL
60 70 80 90 100
TARDFQKLER EARICRKLHH PNIVRLHDSI QEENYHYLVF DLVTGGELFE
110 120 130 140 150
DIVAREFYSE ADASHCIQQI LESVNHCHQN GVVHRDLKPE NLLLASKAKG
160 170 180 190 200
AAVKLADFGL AIEVQGDHQA WFGFAGTPGY LSPEVLKKEP YGKSVDIWAC
210 220 230 240 250
GVILYILLVG YPPFWDEDQH RLYSQIKAGA YDYPSPEWDT VTPEAKNLIN
260 270 280 290 300
QMLTVNPNKR ITAAEALKHP WICQRERVAS VVHRQETVDC LKKFNARRKL
310 320 330 340 350
KGAILTTMLA TRNFSSRSMI TKKGEGSQVK ESTDSSSTTL EDDDIKEDKK
360 370 380 390 400
GTVDRSTTVV SKEPEDIRIL CPAKTYQQNI GNSQCSSARR QEIIKITEQL
410 420 430 440 450
IEAINSGDFD GYTKICDPHL TAFEPEALGN LVEGIDFHKF YFENVLGKNC
460 470 480 490 500
KAINTTILNP HVHLLGEEAA CIAYVRLTQY IDKQGHAHTH QSEETRVWHK
510 520 530
RDNKWQNVHF HRSASAKISG ATTFDFIPQK
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA4V133 | A4V133_DROME | Calcium/calmodulin-dependent protei... | CaMKII Ca2+, Calcium, calcium, CaM, Cam | 490 | Annotation score: | ||
D1YSG7 | D1YSG7_DROME | Calcium/calmodulin-dependent protei... | CaMKII Ca2+, Calcium, calcium, CaM, Cam | 530 | Annotation score: | ||
A4V134 | A4V134_DROME | Calcium/calmodulin-dependent protei... | CaMKII Ca2+, Calcium, calcium, CaM, Cam | 509 | Annotation score: | ||
D1YSG8 | D1YSG8_DROME | Calcium/calmodulin-dependent protei... | CaMKII Ca2+, Calcium, calcium, CaM, Cam | 531 | Annotation score: | ||
L0MNA2 | L0MNA2_DROME | Calcium/calmodulin-dependent protei... | CaMKII Ca2+, Calcium, calcium, CaM, Cam | 482 | Annotation score: | ||
L0MLP2 | L0MLP2_DROME | Calcium/calmodulin-dependent protei... | CaMKII Ca2+, Calcium, calcium, CaM, Cam | 504 | Annotation score: | ||
L0MLR4 | L0MLR4_DROME | Calcium/calmodulin-dependent protei... | CaMKII Ca2+, Calcium, calcium, CaM, Cam | 522 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 388 | A → S in AAB28246 (PubMed:1910789).Curated | 1 | |
Sequence conflicti | 388 | A → S in AAB28248 (PubMed:1910789).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050261 | 347 – 386 | Missing in isoform 4. 2 PublicationsAdd BLAST | 40 | |
Alternative sequenceiVSP_050262 | 366 – 387 | DIRIL…SQCSS → VNLFTNKA in isoform 2. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_050263 | 366 – 387 | DIRIL…SQCSS → A in isoform 3. 1 PublicationAdd BLAST | 22 | |
Alternative sequenceiVSP_050264 | 387 | S → A in isoform 4. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13330 mRNA Translation: BAA02593.1 D13331 mRNA Translation: BAA02594.1 D13332 mRNA Translation: BAA02595.1 D13333 mRNA Translation: BAA02596.1 M74583 mRNA Translation: AAA51459.1 AE014135 Genomic DNA Translation: AAF59388.3 AE014135 Genomic DNA Translation: AAF59390.2 AE014135 Genomic DNA Translation: AAN06568.2 AE014135 Genomic DNA Translation: AAX53595.1 S65712 mRNA Translation: AAB28244.1 S65716 mRNA Translation: AAB28245.1 S65717 mRNA Translation: AAB28246.2 S65719 mRNA Translation: AAB28247.1 S65724 mRNA Translation: AAB28248.2 |
PIRi | B44412 C44412, JU0270 D44412 |
RefSeqi | NP_001014696.1, NM_001014696.2 [Q00168-2] NP_001162831.1, NM_001169360.2 [Q00168-4] NP_001162832.1, NM_001169361.2 [Q00168-1] NP_524635.3, NM_079896.4 [Q00168-4] NP_726633.2, NM_166810.5 [Q00168-4] NP_726634.1, NM_166811.2 [Q00168-3] NP_726635.2, NM_166812.3 [Q00168-1] NP_726636.2, NM_166813.3 [Q00168-3] |
Genome annotation databases
EnsemblMetazoai | FBtr0089217; FBpp0088281; FBgn0264607 [Q00168-4] FBtr0089218; FBpp0088282; FBgn0264607 [Q00168-4] FBtr0089219; FBpp0088283; FBgn0264607 [Q00168-3] FBtr0100146; FBpp0099496; FBgn0264607 [Q00168-1] FBtr0100147; FBpp0099497; FBgn0264607 [Q00168-3] FBtr0100148; FBpp0099498; FBgn0264607 [Q00168-2] FBtr0300377; FBpp0289606; FBgn0264607 [Q00168-4] FBtr0300378; FBpp0289607; FBgn0264607 [Q00168-1] |
GeneIDi | 43828 |
KEGGi | dme:Dmel_CG18069 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia Calmodulin-dependent kinase entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13330 mRNA Translation: BAA02593.1 D13331 mRNA Translation: BAA02594.1 D13332 mRNA Translation: BAA02595.1 D13333 mRNA Translation: BAA02596.1 M74583 mRNA Translation: AAA51459.1 AE014135 Genomic DNA Translation: AAF59388.3 AE014135 Genomic DNA Translation: AAF59390.2 AE014135 Genomic DNA Translation: AAN06568.2 AE014135 Genomic DNA Translation: AAX53595.1 S65712 mRNA Translation: AAB28244.1 S65716 mRNA Translation: AAB28245.1 S65717 mRNA Translation: AAB28246.2 S65719 mRNA Translation: AAB28247.1 S65724 mRNA Translation: AAB28248.2 |
PIRi | B44412 C44412, JU0270 D44412 |
RefSeqi | NP_001014696.1, NM_001014696.2 [Q00168-2] NP_001162831.1, NM_001169360.2 [Q00168-4] NP_001162832.1, NM_001169361.2 [Q00168-1] NP_524635.3, NM_079896.4 [Q00168-4] NP_726633.2, NM_166810.5 [Q00168-4] NP_726634.1, NM_166811.2 [Q00168-3] NP_726635.2, NM_166812.3 [Q00168-1] NP_726636.2, NM_166813.3 [Q00168-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5FG8 | X-ray | 1.96 | A | 1-283 | [»] | |
5H9B | X-ray | 2.25 | A | 1-283 | [»] | |
5HU3 | X-ray | 1.89 | A | 1-283 | [»] | |
SMRi | Q00168 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 68654, 31 interactors |
IntActi | Q00168, 5 interactors |
MINTi | Q00168 |
STRINGi | 7227.FBpp0289608 |
PTM databases
iPTMneti | Q00168 |
Proteomic databases
PaxDbi | Q00168 |
Protocols and materials databases
DNASUi | 43828 |
Genome annotation databases
EnsemblMetazoai | FBtr0089217; FBpp0088281; FBgn0264607 [Q00168-4] FBtr0089218; FBpp0088282; FBgn0264607 [Q00168-4] FBtr0089219; FBpp0088283; FBgn0264607 [Q00168-3] FBtr0100146; FBpp0099496; FBgn0264607 [Q00168-1] FBtr0100147; FBpp0099497; FBgn0264607 [Q00168-3] FBtr0100148; FBpp0099498; FBgn0264607 [Q00168-2] FBtr0300377; FBpp0289606; FBgn0264607 [Q00168-4] FBtr0300378; FBpp0289607; FBgn0264607 [Q00168-1] |
GeneIDi | 43828 |
KEGGi | dme:Dmel_CG18069 |
Organism-specific databases
CTDi | 43828 |
FlyBasei | FBgn0264607, CaMKII |
VEuPathDBi | VectorBase:FBgn0264607 |
Phylogenomic databases
eggNOGi | KOG0033, Eukaryota |
GeneTreei | ENSGT00940000159769 |
InParanoidi | Q00168 |
PhylomeDBi | Q00168 |
Enzyme and pathway databases
BRENDAi | 2.7.11.17, 1994 |
Reactomei | R-DME-3371571, HSF1-dependent transactivation R-DME-4086398, Ca2+ pathway R-DME-438066, Unblocking of NMDA receptors, glutamate binding and activation R-DME-5578775, Ion homeostasis R-DME-5673000, RAF activation R-DME-936837, Ion transport by P-type ATPases |
SignaLinki | Q00168 |
Miscellaneous databases
BioGRID-ORCSi | 43828, 0 hits in 3 CRISPR screens |
GenomeRNAii | 43828 |
PROi | PR:Q00168 |
Gene expression databases
Bgeei | FBgn0264607, Expressed in head and 55 other tissues |
ExpressionAtlasi | Q00168, baseline and differential |
Genevisiblei | Q00168, DM |
Family and domain databases
InterProi | View protein in InterPro IPR013543, Ca/CaM-dep_prot_kinase-assoc IPR011009, Kinase-like_dom_sf IPR032710, NTF2-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF08332, CaMKII_AD, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF54427, SSF54427, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KCC2A_DROME | |
Accessioni | Q00168Primary (citable) accession number: Q00168 Secondary accession number(s): Q59DP1, Q59DP2, Q9V495 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2004 |
Last sequence update: | November 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families