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UniProtKB - Q00168 (KCC2A_DROME)

Protein

Calcium/calmodulin-dependent protein kinase type II alpha chain

Gene

CaMKII

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A key regulator of plasticity in synaptic physiology and behavior, alterations in its activity produce pleiotrophic effects that involve synaptic transmission and development as well as various aspects of behavior. Directly modulates eag potassium channels.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

CASK plays a role in regulation of CaMKII autophosphorylation. When complexed with CASK and in the presence Ca[2+]/CaM, autophosphorylation of Thr-287 causes constitutive activation of the kinase. In the absence of Ca[2+]/CaM, autophosphorylation of Thr-306 causes inactivation of the kinase.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin binding Source: GO_Central
  • calmodulin-dependent protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.17 1994
ReactomeiR-DME-4086398 Ca2+ pathway
R-DME-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-DME-442729 CREB phosphorylation through the activation of CaMKII
R-DME-442742 CREB phosphorylation through the activation of Ras
R-DME-5578775 Ion homeostasis
R-DME-936837 Ion transport by P-type ATPases
SignaLinkiQ00168

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type II alpha chain (EC:2.7.11.17)
Short name:
CaM-kinase II alpha chain
Gene namesi
Name:CaMKII
Synonyms:CaM
ORF Names:CG18069
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora ›
Proteomesi
  • UP000000803 Componenti: Chromosome 4

Organism-specific databases

FlyBaseiFBgn0264607 CaMKII

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi306T → A: Fails to interact with CASK, catalytically active but fails to autophosphorylate, when associated with A-307. 1 Publication1
Mutagenesisi306T → D: Fails to interact with CASK, kinase inactive, when associated with D-307. 1 Publication1
Mutagenesisi306T → S: Fails to interact with CASK, catalytically active and can autophosphorylate, when associated with S-307. 1 Publication1
Mutagenesisi307T → A: Fails to interact with CASK, catalytically active but fails to autophosphorylate, when associated with A-306. 1 Publication1
Mutagenesisi307T → D: Fails to interact with CASK, kinase inactive, when associated with D-306. 1 Publication1
Mutagenesisi307T → S: Fails to interact with CASK, catalytically active and can autophosphorylate, when associated with S-306. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860951 – 530Calcium/calmodulin-dependent protein kinase type II alpha chainAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei287Phosphothreonine; by autocatalysis2 Publications1
Modified residuei306Phosphothreonine; by autocatalysis1 Publication1
Modified residuei307Phosphothreonine; by autocatalysis1 Publication1
Modified residuei327Phosphoserine1 Publication1

Post-translational modificationi

Autophosphorylation at Thr-287 is independent of autophosphorylation at Thr-306 and Thr-307.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ00168
PRIDEiQ00168

PTM databases

iPTMnetiQ00168

Expressioni

Tissue specificityi

Expressed at a high level in the central nervous system during the late embryonic stage. In adults, expression is more abundant in the head than in the body.2 Publications

Gene expression databases

BgeeiFBgn0264607 Expressed in 50 organ(s), highest expression level in head
ExpressionAtlasiQ00168 baseline and differential
GenevisibleiQ00168 DM

Interactioni

Subunit structurei

Interacts with CASK.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CASKQ242105EBI-124595,EBI-214423

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi68654, 24 interactors
IntActiQ00168, 5 interactors
MINTiQ00168
STRINGi7227.FBpp0289608

Structurei

Secondary structure

1530
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ00168
SMRiQ00168
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST261

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni291 – 301Calmodulin-bindingAdd BLAST11

Sequence similaritiesi

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Curated

Phylogenomic databases

eggNOGiKOG0033 Eukaryota
ENOG410XNRX LUCA
GeneTreeiENSGT00760000118944
InParanoidiQ00168
KOiK04515
OrthoDBiEOG091G0SCS
PhylomeDBiQ00168

Family and domain databases

InterProiView protein in InterPro
IPR013543 Ca/CaM-dep_prot_kinase-assoc
IPR039071 CAMK2
IPR011009 Kinase-like_dom_sf
IPR032710 NTF2-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR43940 PTHR43940, 1 hit
PfamiView protein in Pfam
PF08332 CaMKII_AD, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q00168-1) [UniParc]FASTAAdd to basket
Also known as: 530aa, D

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAPAACTRF SDNYDIKEEL GKGAFSIVKR CVQKSTGFEF AAKIINTKKL 
        60         70         80         90        100
TARDFQKLER EARICRKLHH PNIVRLHDSI QEENYHYLVF DLVTGGELFE 
       110        120        130        140        150
DIVAREFYSE ADASHCIQQI LESVNHCHQN GVVHRDLKPE NLLLASKAKG 
       160        170        180        190        200
AAVKLADFGL AIEVQGDHQA WFGFAGTPGY LSPEVLKKEP YGKSVDIWAC 
       210        220        230        240        250
GVILYILLVG YPPFWDEDQH RLYSQIKAGA YDYPSPEWDT VTPEAKNLIN 
       260        270        280        290        300
QMLTVNPNKR ITAAEALKHP WICQRERVAS VVHRQETVDC LKKFNARRKL 
       310        320        330        340        350
KGAILTTMLA TRNFSSRSMI TKKGEGSQVK ESTDSSSTTL EDDDIKEDKK 
       360        370        380        390        400
GTVDRSTTVV SKEPEDIRIL CPAKTYQQNI GNSQCSSARR QEIIKITEQL 
       410        420        430        440        450
IEAINSGDFD GYTKICDPHL TAFEPEALGN LVEGIDFHKF YFENVLGKNC 
       460        470        480        490        500
KAINTTILNP HVHLLGEEAA CIAYVRLTQY IDKQGHAHTH QSEETRVWHK 
       510        520        530
RDNKWQNVHF HRSASAKISG ATTFDFIPQK
Length:530
Mass (Da):59,920
Last modified:November 1, 1996 - v1
Checksum:i4F3D83582ABDFCFD
GO
Isoform 2 (identifier: Q00168-2) [UniParc]FASTAAdd to basket
Also known as: 516aa, G

The sequence of this isoform differs from the canonical sequence as follows:
     366-387: DIRILCPAKTYQQNIGNSQCSS → VNLFTNKA

Show »
Length:516
Mass (Da):58,386
Checksum:i16D891160BEC6FF2
GO
Isoform 3 (identifier: Q00168-3) [UniParc]FASTAAdd to basket
Also known as: 509aa, B, E

The sequence of this isoform differs from the canonical sequence as follows:
     366-387: DIRILCPAKTYQQNIGNSQCSS → A

Show »
Length:509
Mass (Da):57,569
Checksum:iE148946CEA1E057F
GO
Isoform 4 (identifier: Q00168-4) [UniParc]FASTAAdd to basket
Also known as: 490aa, A, C

The sequence of this isoform differs from the canonical sequence as follows:
     347-386: Missing.
     387-387: S → A

Show »
Length:490
Mass (Da):55,482
Checksum:i17E1972584F9B2C1
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A4V133A4V133_DROME
Calcium/calmodulin-dependent protei...
CaMKII Ca2+, Calcium, calcium, CaM, Cam
490Annotation score:
L0MNA2L0MNA2_DROME
Calcium/calmodulin-dependent protei...
CaMKII Ca2+, Calcium, calcium, CaM, Cam
482Annotation score:
D1YSG7D1YSG7_DROME
Calcium/calmodulin-dependent protei...
CaMKII Ca2+, Calcium, calcium, CaM, Cam
530Annotation score:
A4V134A4V134_DROME
Calcium/calmodulin-dependent protei...
CaMKII Ca2+, Calcium, calcium, CaM, Cam
509Annotation score:
D1YSG8D1YSG8_DROME
Calcium/calmodulin-dependent protei...
CaMKII Ca2+, Calcium, calcium, CaM, Cam
531Annotation score:
L0MLR4L0MLR4_DROME
Calcium/calmodulin-dependent protei...
CaMKII Ca2+, Calcium, calcium, CaM, Cam
522Annotation score:
L0MLP2L0MLP2_DROME
Calcium/calmodulin-dependent protei...
CaMKII Ca2+, Calcium, calcium, CaM, Cam
504Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti388A → S in AAB28246 (PubMed:1910789).Curated1
Sequence conflicti388A → S in AAB28248 (PubMed:1910789).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050261347 – 386Missing in isoform 4. 2 PublicationsAdd BLAST40
Alternative sequenceiVSP_050262366 – 387DIRIL…SQCSS → VNLFTNKA in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_050263366 – 387DIRIL…SQCSS → A in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_050264387S → A in isoform 4. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13330 mRNA Translation: BAA02593.1
D13331 mRNA Translation: BAA02594.1
D13332 mRNA Translation: BAA02595.1
D13333 mRNA Translation: BAA02596.1
M74583 mRNA Translation: AAA51459.1
AE014135 Genomic DNA Translation: AAF59388.3
AE014135 Genomic DNA Translation: AAF59390.2
AE014135 Genomic DNA Translation: AAN06568.2
AE014135 Genomic DNA Translation: AAX53595.1
S65712 mRNA Translation: AAB28244.1
S65716 mRNA Translation: AAB28245.1
S65717 mRNA Translation: AAB28246.2
S65719 mRNA Translation: AAB28247.1
S65724 mRNA Translation: AAB28248.2
PIRiB44412
C44412 JU0270
D44412
RefSeqiNP_001014696.1, NM_001014696.2 [Q00168-2]
NP_001162831.1, NM_001169360.2 [Q00168-4]
NP_001162832.1, NM_001169361.2 [Q00168-1]
NP_524635.3, NM_079896.4 [Q00168-4]
NP_726633.2, NM_166810.5 [Q00168-4]
NP_726634.1, NM_166811.2 [Q00168-3]
NP_726635.2, NM_166812.3 [Q00168-1]
NP_726636.2, NM_166813.3 [Q00168-3]
UniGeneiDm.1709

Genome annotation databases

EnsemblMetazoaiFBtr0089217; FBpp0088281; FBgn0264607 [Q00168-4]
FBtr0089218; FBpp0088282; FBgn0264607 [Q00168-4]
FBtr0089219; FBpp0088283; FBgn0264607 [Q00168-3]
FBtr0100146; FBpp0099496; FBgn0264607 [Q00168-1]
FBtr0100147; FBpp0099497; FBgn0264607 [Q00168-3]
FBtr0100148; FBpp0099498; FBgn0264607 [Q00168-2]
FBtr0300377; FBpp0289606; FBgn0264607 [Q00168-4]
FBtr0300378; FBpp0289607; FBgn0264607 [Q00168-1]
GeneIDi43828
KEGGidme:Dmel_CG18069

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Calmodulin-dependent kinase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13330 mRNA Translation: BAA02593.1
D13331 mRNA Translation: BAA02594.1
D13332 mRNA Translation: BAA02595.1
D13333 mRNA Translation: BAA02596.1
M74583 mRNA Translation: AAA51459.1
AE014135 Genomic DNA Translation: AAF59388.3
AE014135 Genomic DNA Translation: AAF59390.2
AE014135 Genomic DNA Translation: AAN06568.2
AE014135 Genomic DNA Translation: AAX53595.1
S65712 mRNA Translation: AAB28244.1
S65716 mRNA Translation: AAB28245.1
S65717 mRNA Translation: AAB28246.2
S65719 mRNA Translation: AAB28247.1
S65724 mRNA Translation: AAB28248.2
PIRiB44412
C44412 JU0270
D44412
RefSeqiNP_001014696.1, NM_001014696.2 [Q00168-2]
NP_001162831.1, NM_001169360.2 [Q00168-4]
NP_001162832.1, NM_001169361.2 [Q00168-1]
NP_524635.3, NM_079896.4 [Q00168-4]
NP_726633.2, NM_166810.5 [Q00168-4]
NP_726634.1, NM_166811.2 [Q00168-3]
NP_726635.2, NM_166812.3 [Q00168-1]
NP_726636.2, NM_166813.3 [Q00168-3]
UniGeneiDm.1709

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FG8X-ray1.96A1-283[»]
5H9BX-ray2.25A1-283[»]
5HU3X-ray1.89A1-283[»]
ProteinModelPortaliQ00168
SMRiQ00168
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68654, 24 interactors
IntActiQ00168, 5 interactors
MINTiQ00168
STRINGi7227.FBpp0289608

PTM databases

iPTMnetiQ00168

Proteomic databases

PaxDbiQ00168
PRIDEiQ00168

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0089217; FBpp0088281; FBgn0264607 [Q00168-4]
FBtr0089218; FBpp0088282; FBgn0264607 [Q00168-4]
FBtr0089219; FBpp0088283; FBgn0264607 [Q00168-3]
FBtr0100146; FBpp0099496; FBgn0264607 [Q00168-1]
FBtr0100147; FBpp0099497; FBgn0264607 [Q00168-3]
FBtr0100148; FBpp0099498; FBgn0264607 [Q00168-2]
FBtr0300377; FBpp0289606; FBgn0264607 [Q00168-4]
FBtr0300378; FBpp0289607; FBgn0264607 [Q00168-1]
GeneIDi43828
KEGGidme:Dmel_CG18069

Organism-specific databases

CTDi43828
FlyBaseiFBgn0264607 CaMKII

Phylogenomic databases

eggNOGiKOG0033 Eukaryota
ENOG410XNRX LUCA
GeneTreeiENSGT00760000118944
InParanoidiQ00168
KOiK04515
OrthoDBiEOG091G0SCS
PhylomeDBiQ00168

Enzyme and pathway databases

BRENDAi2.7.11.17 1994
ReactomeiR-DME-4086398 Ca2+ pathway
R-DME-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-DME-442729 CREB phosphorylation through the activation of CaMKII
R-DME-442742 CREB phosphorylation through the activation of Ras
R-DME-5578775 Ion homeostasis
R-DME-936837 Ion transport by P-type ATPases
SignaLinkiQ00168

Miscellaneous databases

GenomeRNAii43828
PROiPR:Q00168

Gene expression databases

BgeeiFBgn0264607 Expressed in 50 organ(s), highest expression level in head
ExpressionAtlasiQ00168 baseline and differential
GenevisibleiQ00168 DM

Family and domain databases

InterProiView protein in InterPro
IPR013543 Ca/CaM-dep_prot_kinase-assoc
IPR039071 CAMK2
IPR011009 Kinase-like_dom_sf
IPR032710 NTF2-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR43940 PTHR43940, 1 hit
PfamiView protein in Pfam
PF08332 CaMKII_AD, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKCC2A_DROME
AccessioniPrimary (citable) accession number: Q00168
Secondary accession number(s): Q59DP1, Q59DP2, Q9V495
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: November 1, 1996
Last modified: November 7, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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