cprA

Functionⁱ

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.UniRule annotation

Catalytic activityⁱ

NADPH + n oxidized hemoprotein = NADP⁺ + n reduced hemoprotein.UniRule annotation2 Publications

Cofactorⁱ

Protein has several cofactor binding sites:

FAD
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_03212
2 Publications
Manual assertion based on experiment inⁱ
- Ref.1
  "Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger."
  van den Brink H.J.M., van Zeijl C.M.J., Brons J.F., van den Hondel C.A.M.J.J., van Gorcom R.F.M.
  DNA Cell Biol. 14:719-729(1995) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, INDUCTION.
- Ref.2
  "Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger."
  Faber B.W., van Gorcom R.F.M., Duine J.A.
  Arch. Biochem. Biophys. 394:245-254(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: CATALYTIC ACTIVITY.
Note: Binds 1 FAD per monomer.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_03212
FMN
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_03212
2 Publications
Manual assertion based on experiment inⁱ
- Ref.1
  "Cloning and characterization of the NADPH cytochrome P450 oxidoreductase gene from the filamentous fungus Aspergillus niger."
  van den Brink H.J.M., van Zeijl C.M.J., Brons J.F., van den Hondel C.A.M.J.J., van Gorcom R.F.M.
  DNA Cell Biol. 14:719-729(1995) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, INDUCTION.
- Ref.2
  "Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger."
  Faber B.W., van Gorcom R.F.M., Duine J.A.
  Arch. Biochem. Biophys. 394:245-254(2001) [PubMed] [Europe PMC] [Abstract]
  Cited for: CATALYTIC ACTIVITY.
Note: Binds 1 FMN per monomer.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_03212

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	203	FMNUniRule annotation	1
Binding siteⁱ	295	NADPUniRule annotation	1
Binding siteⁱ	551	NADPUniRule annotation	1
Binding siteⁱ	655	NADPUniRule annotation	1
Binding siteⁱ	693	FADUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	72 – 77	FMNUniRule annotation	6
Nucleotide bindingⁱ	123 – 126	FMNUniRule annotation	4
Nucleotide bindingⁱ	168 – 177	FMNUniRule annotation	10
Nucleotide bindingⁱ	450 – 453	FADUniRule annotation	4
Nucleotide bindingⁱ	468 – 470	FADUniRule annotation	3
Nucleotide bindingⁱ	485 – 488	FADUniRule annotation	4
Nucleotide bindingⁱ	613 – 614	NADPUniRule annotation	2
Nucleotide bindingⁱ	619 – 623	NADPUniRule annotation	5

GO - Molecular functionⁱ

electron transfer activity
Source: UniProtKB
Inferred from direct assayⁱ
- 7646819
flavin adenine dinucleotide binding Source: UniProtKB-UniRule
FMN binding Source: UniProtKB-UniRule
NADP binding Source: UniProtKB-UniRule
NADPH-hemoprotein reductase activity Source: UniProtKB-UniRule
oxidoreductase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11594739

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ergosterol biosynthetic process Source: UniProtKB-UniRule
oxidation-reduction process
Source: UniProtKB
Inferred from direct assayⁱ
- 7646819

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
Ligand	FAD, Flavoprotein, FMN, NADP

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation) Short name: CPRUniRule annotation Short name: P450RUniRule annotation
Gene namesⁱ	Name:cprAUniRule annotation
Organismⁱ	Aspergillus nigerImported
Taxonomic identifierⁱ	5061 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Aspergillaceae › Aspergillus

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 8	LumenalUniRule annotation	8
Transmembraneⁱ	9 – 31	HelicalUniRule annotationAdd BLAST	23
Topological domainⁱ	32 – 694	CytoplasmicUniRule annotationAdd BLAST	663

Keywords - Cellular componentⁱ

Cell membrane, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000167604	1 – 694	NADPH--cytochrome P450 reductaseAdd BLAST		694

Proteomic databases

PRIDEⁱ	Q00141

PRIDEⁱ

Q00141

Expressionⁱ

Inductionⁱ

By benzoic acid (BA).1 Publication

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q00141
SMRⁱ	Q00141
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	66 – 220	Flavodoxin-likeUniRule annotationAdd BLAST		155
Domainⁱ	276 – 537	FAD-binding FR-typeUniRule annotationAdd BLAST		262

Sequence similaritiesⁱ

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation

In the N-terminal section; belongs to the flavodoxin family.UniRule annotation

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG1158 Eukaryota COG0369 LUCA

eggNOGⁱ

KOG1158 Eukaryota
COG0369 LUCA

Family and domain databases

Gene3Dⁱ	1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit
HAMAPⁱ	MF_03212 NCPR, 1 hit
InterProⁱ	View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit
PIRSFⁱ	PIRSF000208 P450R, 1 hit
PRINTSⁱ	PR00369 FLAVODOXIN PR00371 FPNCR
SUPFAMⁱ	SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit
PROSITEⁱ	View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q00141-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAQLDTLDLV VLAVLLVGSV AYFTKGTYWA VAKDPYASTG PAMNGAAKAG 
        60         70         80         90        100
KTRNIIEKME ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD 
       110        120        130        140        150
LEEYDYENLD QFPEDKVAFF VLATYGEGEP TDNAVEFYQF FTGDDVAFES 
       160        170        180        190        200
ASADEKPLSK LKYVAFGLGN NTYEHYNAMV RQVDAAFQKL GPQRIGSAGE 
       210        220        230        240        250
GDDGAGTMEE DFLAWKEPMW AALSESMDLE EREAVYEPVF CVTENESLSP 
       260        270        280        290        300
EDETVYLGEP TQSHLQGTPK GPYSAHNPFI APIAESRELF TVKDRNCLHM 
       310        320        330        340        350
EISIAGSNLS YQTGDHIAVW PTNAGAEVDR FLQVFGLEGK RDSVINIKGI 
       360        370        380        390        400
DVTAKVPIPT PTTYDAAVRY YMEVCAPVSR QFVATLAAFA PDEESKAEIV 
       410        420        430        440        450
RLGSHKDYFH EKVTNQCFNM AQALQSITSK PFSAVPFSLL IEGITKLQPR 
       460        470        480        490        500
YYSISSSSLV QKDKISITAV VESVRLPGAS HMVKGVTTNY LLALKQKQNG 
       510        520        530        540        550
DPSPDPHGLT YSITGPRNKY DGIHVPVHVR HSNFKLPSDP SRPIIMVGPG 
       560        570        580        590        600
TGVAPFRGFI QERAALAAKG EKVGPTVLFF GCRKSDEDFL YKDEWKTYQD 
       610        620        630        640        650
QLGDNLKIIT AFSREGPQKV YVQHRLREHS ELVSDLLKQK ATFYVCGDAA 
       660        670        680        690 
NMAREVNLVL GQIIAAQRGL PAEKGEEMVK HMRRRGRYQE DVWS

Length:694

Mass (Da):76,807

Last modified:February 8, 2011 - v2

Checksum:ⁱBA3E7867ACB1D810

GO

Sequence cautionⁱ

The sequence CAA81550 differs from that shown. Reason: Frameshift at positions 34, 43, 392, 405, 421, 501 and 515.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z26938 Genomic DNA Translation: CAA81550.1 Frameshift.
PIRⁱ	S38427

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q00141	NADPH--cytochrome P450 reductase		Aspergillus aculeatus ATCC 16872		695	UniRef90_Q00141
NADPH--cytochrome P450 reductase		Aspergillus indologenus CBS 114.80		695
UPI000DBCECDF		Aspergillus japonicus CBS 114.51		695
UPI000DBDACC1		Aspergillus brunneoviolaceus CBS 621.78		695
UPI000DBDF677		Aspergillus aculeatinus CBS 121060		695

Protein	Similar proteins		Species	Score	Length	Source
Q00141	NADPH--cytochrome P450 reductase		ASPTN		695	UniRef50_Q00141
NADPH--cytochrome P450 reductase		ASPNC		695
NADPH--cytochrome P450 reductase		ASPOR		695
NADPH--cytochrome P450 reductase		Aspergillus indologenus CBS 114.80		695
NADPH--cytochrome P450 reductase		Aspergillus aculeatus ATCC 16872		695
+303

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z26938 Genomic DNA Translation: CAA81550.1 Frameshift.
PIRⁱ	S38427

3D structure databases

ProteinModelPortalⁱ	Q00141
SMRⁱ	Q00141
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PRIDEⁱ	Q00141

PRIDEⁱ

Q00141

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Phylogenomic databases

eggNOGⁱ	KOG1158 Eukaryota COG0369 LUCA

eggNOGⁱ

KOG1158 Eukaryota
COG0369 LUCA

Family and domain databases

Gene3Dⁱ	1.20.990.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit
HAMAPⁱ	MF_03212 NCPR, 1 hit
InterProⁱ	View protein in InterPro IPR003097 CysJ-like_FAD-binding IPR017927 FAD-bd_FR_type IPR001094 Flavdoxin-like IPR008254 Flavodoxin/NO_synth IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039 Flavoprotein-like_sf IPR039261 FNR_nucleotide-bd IPR023173 NADPH_Cyt_P450_Rdtase_alpha IPR001433 OxRdtase_FAD/NAD-bd IPR023208 P450R IPR017938 Riboflavin_synthase-like_b-brl
Pfamⁱ	View protein in Pfam PF00667 FAD_binding_1, 1 hit PF00258 Flavodoxin_1, 1 hit PF00175 NAD_binding_1, 1 hit
PIRSFⁱ	PIRSF000208 P450R, 1 hit
PRINTSⁱ	PR00369 FLAVODOXIN PR00371 FPNCR
SUPFAMⁱ	SSF52218 SSF52218, 1 hit SSF52343 SSF52343, 1 hit SSF63380 SSF63380, 1 hit
PROSITEⁱ	View protein in PROSITE PS51384 FAD_FR, 1 hit PS50902 FLAVODOXIN_LIKE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NCPR_ASPNG
Accessionⁱ	Q00141Primary (citable) accession number: Q00141
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
	Last sequence update:	February 8, 2011
	Last modified:	October 10, 2018
	This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Documents

SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q00141 (NCPR_ASPNG)

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NADPH--cytochrome P450 reductase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Mitochondrion

Endoplasmic reticulum

Endoplasmic reticulum

Mitochondrion

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ