UniProtKB - Q00013 (EM55_HUMAN)
Protein
55 kDa erythrocyte membrane protein
Gene
MPP1
Organism
Homo sapiens (Human)
Status
Functioni
Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity).By similarity
Caution
Thought to be palmitoylated by ZDHHC17 (PubMed:22496366). This work was later retracted due to image manipulation (PubMed:29475958).2 Publications
GO - Molecular functioni
- guanylate kinase activity Source: ProtInc
GO - Biological processi
- regulation of neutrophil chemotaxis Source: UniProtKB
- signal transduction Source: ProtInc
Enzyme and pathway databases
PathwayCommonsi | Q00013 |
Names & Taxonomyi
Protein namesi | Recommended name: 55 kDa erythrocyte membrane proteinShort name: p55 Alternative name(s): Membrane protein, palmitoylated 1 |
Gene namesi | Name:MPP1 Synonyms:DXS552E, EMP55 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:7219, MPP1 |
MIMi | 305360, gene |
neXtProti | NX_Q00013 |
VEuPathDBi | HostDB:ENSG00000130830.14 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Other locations
- stereocilium By similarity
Note: Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with MPP5 in the retina, at the outer limiting membrane (OLM) (By similarity). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC) (By similarity). Colocalizes with NF2 in non-myelin-forming Schwann cells (PubMed:19144871).By similarity1 Publication
Cytoskeleton
- centriolar satellite Source: HPA
- cortical cytoskeleton Source: Ensembl
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- membrane Source: UniProtKB
- stereocilium Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 4354 |
OpenTargetsi | ENSG00000130830 |
PharmGKBi | PA30924 |
Miscellaneous databases
Pharosi | Q00013, Tbio |
Genetic variation databases
BioMutai | MPP1 |
DMDMi | 1346575 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000094565 | 2 – 466 | 55 kDa erythrocyte membrane proteinAdd BLAST | 465 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineCombined sources | 1 | |
Modified residuei | 13 | PhosphoserineCombined sources | 1 | |
Modified residuei | 19 | PhosphoserineCombined sources | 1 | |
Modified residuei | 49 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 52 | PhosphoserineBy similarity | 1 | |
Modified residuei | 57 | PhosphoserineCombined sources | 1 | |
Modified residuei | 110 | PhosphoserineCombined sources | 1 | |
Modified residuei | 243 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Palmitoylated.1 Publication
Keywords - PTMi
Acetylation, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | Q00013 |
jPOSTi | Q00013 |
MassIVEi | Q00013 |
PaxDbi | Q00013 |
PeptideAtlasi | Q00013 |
PRIDEi | Q00013 |
ProteomicsDBi | 33755 57837 [Q00013-1] 57838 [Q00013-2] |
PTM databases
iPTMneti | Q00013 |
MetOSitei | Q00013 |
PhosphoSitePlusi | Q00013 |
SwissPalmi | Q00013 |
Expressioni
Tissue specificityi
Ubiquitous.1 Publication
Gene expression databases
Bgeei | ENSG00000130830, Expressed in blood and 245 other tissues |
ExpressionAtlasi | Q00013, baseline and differential |
Genevisiblei | Q00013, HS |
Organism-specific databases
HPAi | ENSG00000130830, Low tissue specificity |
Interactioni
Subunit structurei
Heterodimer with MPP5.
Interacts with DLG5 and NF2.
Interacts (via guanylate kinase-like domain) with WHRN (via third PDZ domain).
2 PublicationsBinary interactionsi
Hide detailsQ00013
Protein-protein interaction databases
BioGRIDi | 110494, 79 interactors |
CORUMi | Q00013 |
ELMi | Q00013 |
IntActi | Q00013, 69 interactors |
MINTi | Q00013 |
STRINGi | 9606.ENSP00000358547 |
Miscellaneous databases
RNActi | Q00013, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q00013 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q00013 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 71 – 152 | PDZPROSITE-ProRule annotationAdd BLAST | 82 | |
Domaini | 158 – 228 | SH3PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 282 – 451 | Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST | 170 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 268 – 466 | Interaction with MPP51 PublicationAdd BLAST | 199 |
Sequence similaritiesi
Belongs to the MAGUK family.Curated
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG0609, Eukaryota |
GeneTreei | ENSGT00940000158744 |
HOGENOMi | CLU_001715_5_1_1 |
InParanoidi | Q00013 |
OMAi | AMTEDIY |
OrthoDBi | 95102at2759 |
PhylomeDBi | Q00013 |
TreeFami | TF314263 |
Family and domain databases
CDDi | cd12080, SH3_MPP1, 1 hit |
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR035475, MPP1_SH3 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 1 hit PS50002, SH3, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q00013-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTLKASEGES GGSMHTALSD LYLEHLLQKR SRPEAVSHPL NTVTEDMYTN
60 70 80 90 100
GSPAPGSPAQ VKGQEVRKVR LIQFEKVTEE PMGITLKLNE KQSCTVARIL
110 120 130 140 150
HGGMIHRQGS LHVGDEILEI NGTNVTNHSV DQLQKAMKET KGMISLKVIP
160 170 180 190 200
NQQSRLPALQ MFMRAQFDYD PKKDNLIPCK EAGLKFATGD IIQIINKDDS
210 220 230 240 250
NWWQGRVEGS SKESAGLIPS PELQEWRVAS MAQSAPSEAP SCSPFGKKKK
260 270 280 290 300
YKDKYLAKHS SIFDQLDVVS YEEVVRLPAF KRKTLVLIGA SGVGRSHIKN
310 320 330 340 350
ALLSQNPEKF VYPVPYTTRP PRKSEEDGKE YHFISTEEMT RNISANEFLE
360 370 380 390 400
FGSYQGNMFG TKFETVHQIH KQNKIAILDI EPQTLKIVRT AELSPFIVFI
410 420 430 440 450
APTDQGTQTE ALQQLQKDSE AIRSQYAHYF DLSLVNNGVD ETLKKLQEAF
460
DQACSSPQWV PVSWVY
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8MTH1 | A8MTH1_HUMAN | 55 kDa erythrocyte membrane protein | MPP1 | 241 | Annotation score: | ||
A6NFY0 | A6NFY0_HUMAN | 55 kDa erythrocyte membrane protein | MPP1 | 131 | Annotation score: | ||
C9JB34 | C9JB34_HUMAN | 55 kDa erythrocyte membrane protein | MPP1 | 156 | Annotation score: | ||
C9J9J4 | C9J9J4_HUMAN | 55 kDa erythrocyte membrane protein | MPP1 | 205 | Annotation score: | ||
F8WC84 | F8WC84_HUMAN | 55 kDa erythrocyte membrane protein | MPP1 | 68 | Annotation score: | ||
F8WDV6 | F8WDV6_HUMAN | 55 kDa erythrocyte membrane protein | MPP1 | 89 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 204 | Q → R in AAV35469 (Ref. 5) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_011914 | 448 | E → Q. Corresponds to variant dbSNP:rs14092Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042675 | 1 – 34 | MTLKA…RSRPE → MESW in isoform 2. 1 PublicationAdd BLAST | 34 | |
Alternative sequenceiVSP_044634 | 161 – 180 | Missing in isoform 3. 1 PublicationAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64925 mRNA Translation: AAA60059.1 M87059 Genomic DNA Translation: AAA60060.1 U39611 Genomic DNA Translation: AAD14835.1 AY423731 mRNA Translation: AAS00494.1 AK290246 mRNA Translation: BAF82935.1 AK303111 mRNA Translation: BAG64217.1 AK312296 mRNA Translation: BAG35223.1 AK315957 mRNA Translation: BAH14328.1 AY634686 mRNA Translation: AAV35469.1 AC109993 Genomic DNA No translation available. CH471172 Genomic DNA Translation: EAW72655.1 CH471172 Genomic DNA Translation: EAW72656.1 CH471172 Genomic DNA Translation: EAW72660.1 BC002392 mRNA Translation: AAH02392.1 |
CCDSi | CCDS14762.1 [Q00013-1] CCDS55544.1 [Q00013-2] CCDS55545.1 [Q00013-3] |
PIRi | A39599 |
RefSeqi | NP_001159932.1, NM_001166460.1 NP_001159933.1, NM_001166461.1 [Q00013-3] NP_001159934.1, NM_001166462.1 [Q00013-2] NP_002427.1, NM_002436.3 [Q00013-1] |
Genome annotation databases
Ensembli | ENST00000369534; ENSP00000358547; ENSG00000130830 [Q00013-1] ENST00000393531; ENSP00000377165; ENSG00000130830 [Q00013-3] ENST00000413259; ENSP00000400155; ENSG00000130830 [Q00013-2] |
GeneIDi | 4354 |
KEGGi | hsa:4354 |
UCSCi | uc004fmp.3, human [Q00013-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M64925 mRNA Translation: AAA60059.1 M87059 Genomic DNA Translation: AAA60060.1 U39611 Genomic DNA Translation: AAD14835.1 AY423731 mRNA Translation: AAS00494.1 AK290246 mRNA Translation: BAF82935.1 AK303111 mRNA Translation: BAG64217.1 AK312296 mRNA Translation: BAG35223.1 AK315957 mRNA Translation: BAH14328.1 AY634686 mRNA Translation: AAV35469.1 AC109993 Genomic DNA No translation available. CH471172 Genomic DNA Translation: EAW72655.1 CH471172 Genomic DNA Translation: EAW72656.1 CH471172 Genomic DNA Translation: EAW72660.1 BC002392 mRNA Translation: AAH02392.1 |
CCDSi | CCDS14762.1 [Q00013-1] CCDS55544.1 [Q00013-2] CCDS55545.1 [Q00013-3] |
PIRi | A39599 |
RefSeqi | NP_001159932.1, NM_001166460.1 NP_001159933.1, NM_001166461.1 [Q00013-3] NP_001159934.1, NM_001166462.1 [Q00013-2] NP_002427.1, NM_002436.3 [Q00013-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EJY | NMR | - | A | 69-153 | [»] | |
2EV8 | NMR | - | A | 69-153 | [»] | |
3NEY | X-ray | 2.26 | A/B/C/D/E/F | 282-460 | [»] | |
SMRi | Q00013 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110494, 79 interactors |
CORUMi | Q00013 |
ELMi | Q00013 |
IntActi | Q00013, 69 interactors |
MINTi | Q00013 |
STRINGi | 9606.ENSP00000358547 |
PTM databases
iPTMneti | Q00013 |
MetOSitei | Q00013 |
PhosphoSitePlusi | Q00013 |
SwissPalmi | Q00013 |
Genetic variation databases
BioMutai | MPP1 |
DMDMi | 1346575 |
Proteomic databases
EPDi | Q00013 |
jPOSTi | Q00013 |
MassIVEi | Q00013 |
PaxDbi | Q00013 |
PeptideAtlasi | Q00013 |
PRIDEi | Q00013 |
ProteomicsDBi | 33755 57837 [Q00013-1] 57838 [Q00013-2] |
Protocols and materials databases
Antibodypediai | 419, 326 antibodies |
DNASUi | 4354 |
Genome annotation databases
Ensembli | ENST00000369534; ENSP00000358547; ENSG00000130830 [Q00013-1] ENST00000393531; ENSP00000377165; ENSG00000130830 [Q00013-3] ENST00000413259; ENSP00000400155; ENSG00000130830 [Q00013-2] |
GeneIDi | 4354 |
KEGGi | hsa:4354 |
UCSCi | uc004fmp.3, human [Q00013-1] |
Organism-specific databases
CTDi | 4354 |
DisGeNETi | 4354 |
GeneCardsi | MPP1 |
HGNCi | HGNC:7219, MPP1 |
HPAi | ENSG00000130830, Low tissue specificity |
MIMi | 305360, gene |
neXtProti | NX_Q00013 |
OpenTargetsi | ENSG00000130830 |
PharmGKBi | PA30924 |
VEuPathDBi | HostDB:ENSG00000130830.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0609, Eukaryota |
GeneTreei | ENSGT00940000158744 |
HOGENOMi | CLU_001715_5_1_1 |
InParanoidi | Q00013 |
OMAi | AMTEDIY |
OrthoDBi | 95102at2759 |
PhylomeDBi | Q00013 |
TreeFami | TF314263 |
Enzyme and pathway databases
PathwayCommonsi | Q00013 |
Miscellaneous databases
BioGRID-ORCSi | 4354, 2 hits in 499 CRISPR screens |
ChiTaRSi | MPP1, human |
EvolutionaryTracei | Q00013 |
GeneWikii | MPP1 |
GenomeRNAii | 4354 |
Pharosi | Q00013, Tbio |
PROi | PR:Q00013 |
RNActi | Q00013, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000130830, Expressed in blood and 245 other tissues |
ExpressionAtlasi | Q00013, baseline and differential |
Genevisiblei | Q00013, HS |
Family and domain databases
CDDi | cd12080, SH3_MPP1, 1 hit |
Gene3Di | 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR035475, MPP1_SH3 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EM55_HUMAN | |
Accessioni | Q00013Primary (citable) accession number: Q00013 Secondary accession number(s): B4DZV5 Q5J7V5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 1992 |
Last sequence update: | February 1, 1996 | |
Last modified: | February 10, 2021 | |
This is version 208 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants