UniProtKB - P9WQP3 (A85A_MYCTU)
Diacylglycerol acyltransferase/mycolyltransferase Ag85A
fbpA
Functioni
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.
4 PublicationsMiscellaneous
Catalytic activityi
Kineticsi
- KM=306 µM for C26:0-CoA (with 1,2-dipalmitin as the acyl acceptor and at 37 degrees Celsius)1 Publication
- KM=390 µM for palmitoleoyl-CoA (with 1,2-dipalmitin as the acyl acceptor and at 37 degrees Celsius)1 Publication
- KM=540 µM for C18:0-CoA (with 1,2-dipalmitin as the acyl acceptor and at 37 degrees Celsius)1 Publication
- Vmax=3166 nmol/min/mg enzyme (at 37 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 169 | NucleophileCurated | 1 | |
Binding sitei | 169 | SubstrateBy similarity | 1 | |
Binding sitei | 197 | SubstrateBy similarity | 1 | |
Active sitei | 273 | By similarity | 1 | |
Binding sitei | 282 | SubstrateBy similarity | 1 | |
Active sitei | 305 | By similarity | 1 |
GO - Molecular functioni
- acyltransferase activity, transferring groups other than amino-acyl groups Source: MTBBASE
- diacylglycerol O-acyltransferase activity Source: UniProtKB-EC
- trehalose O-mycolyltransferase activity Source: UniProtKB-EC
- zymogen binding Source: CAFA
GO - Biological processi
- mycolate cell wall layer assembly Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.1.122, 3445 2.3.1.20, 3445 |
Protein family/group databases
ESTHERi | myctu-a85a, A85-Mycolyl-transferase |
MoonProti | P9WQP3 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fbpA Synonyms:mpt44 Ordered Locus Names:Rv3804c ORF Names:MTV026.09c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3804c |
Subcellular locationi
Cell Wall
- cell wall Source: MTBBASE
Extracellular region or secreted
- extracellular region Source: CAFA
Plasma Membrane
- plasma membrane Source: MTBBASE
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell wall, Cytoplasm, SecretedPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 169 | S → A: Abolishes diacylglycerol acyltransferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 43 | Tat-type signalSequence analysisAdd BLAST | 43 | |
ChainiPRO_0000000214 | 44 – 338 | Diacylglycerol acyltransferase/mycolyltransferase Ag85AAdd BLAST | 295 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 130 ↔ 135 | 2 Publications |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | P9WQP3 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsBinary interactionsi
Diacylglycerol acyltransferase/mycolyltransferase Ag85A (PRO_0000000214)
GO - Molecular functioni
- zymogen binding Source: CAFA
Protein-protein interaction databases
IntActi | P9WQP3, 6 interactors |
STRINGi | 83332.Rv3804c |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P9WQP3 |
SMRi | P9WQP3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 85 – 86 | Substrate bindingBy similarity | 2 | |
Regioni | 101 – 111 | Fibronectin-bindingAdd BLAST | 11 | |
Regioni | 275 – 278 | Substrate bindingBy similarity | 4 | |
Regioni | 305 – 307 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0627, Bacteria |
OMAi | AWARNDP |
PhylomeDBi | P9WQP3 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like IPR006311, TAT_signal |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MQLVDRVRGA VTGMSRRLVV GAVGAALVSG LVGAVGGTAT AGAFSRPGLP
60 70 80 90 100
VEYLQVPSPS MGRDIKVQFQ SGGANSPALY LLDGLRAQDD FSGWDINTPA
110 120 130 140 150
FEWYDQSGLS VVMPVGGQSS FYSDWYQPAC GKAGCQTYKW ETFLTSELPG
160 170 180 190 200
WLQANRHVKP TGSAVVGLSM AASSALTLAI YHPQQFVYAG AMSGLLDPSQ
210 220 230 240 250
AMGPTLIGLA MGDAGGYKAS DMWGPKEDPA WQRNDPLLNV GKLIANNTRV
260 270 280 290 300
WVYCGNGKPS DLGGNNLPAK FLEGFVRTSN IKFQDAYNAG GGHNGVFDFP
310 320 330
DSGTHSWEYW GAQLNAMKPD LQRALGATPN TGPAPQGA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27016 Genomic DNA Translation: AAA50288.1 U47335 Genomic DNA Translation: AAC44295.1 GQ150314 Genomic DNA Translation: ADD50052.1 AL123456 Genomic DNA Translation: CCP46633.1 |
PIRi | H70887 |
RefSeqi | NP_218321.1, NC_000962.3 WP_003900759.1, NZ_NVQJ01000022.1 |
Genome annotation databases
GeneIDi | 45427805 886132 |
KEGGi | mtu:Rv3804c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27016 Genomic DNA Translation: AAA50288.1 U47335 Genomic DNA Translation: AAC44295.1 GQ150314 Genomic DNA Translation: ADD50052.1 AL123456 Genomic DNA Translation: CCP46633.1 |
PIRi | H70887 |
RefSeqi | NP_218321.1, NC_000962.3 WP_003900759.1, NZ_NVQJ01000022.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SFR | X-ray | 2.70 | A/B/C | 43-338 | [»] | |
AlphaFoldDBi | P9WQP3 | |||||
SMRi | P9WQP3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P9WQP3, 6 interactors |
STRINGi | 83332.Rv3804c |
Protein family/group databases
ESTHERi | myctu-a85a, A85-Mycolyl-transferase |
MoonProti | P9WQP3 |
Proteomic databases
PaxDbi | P9WQP3 |
Protocols and materials databases
DNASUi | 886132 |
Genome annotation databases
GeneIDi | 45427805 886132 |
KEGGi | mtu:Rv3804c |
Organism-specific databases
TubercuListi | Rv3804c |
Phylogenomic databases
eggNOGi | COG0627, Bacteria |
OMAi | AWARNDP |
PhylomeDBi | P9WQP3 |
Enzyme and pathway databases
BRENDAi | 2.3.1.122, 3445 2.3.1.20, 3445 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000801, Esterase-like IPR006311, TAT_signal |
Pfami | View protein in Pfam PF00756, Esterase, 1 hit |
SUPFAMi | SSF53474, SSF53474, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | A85A_MYCTU | |
Accessioni | P9WQP3Primary (citable) accession number: P9WQP3 Secondary accession number(s): D6MJP3 P17996 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | May 25, 2022 | |
This is version 41 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families