UniProtKB - P9WQG9 (AAC2_MYCTU)
Protein
Aminoglycoside 2'-N-acetyltransferase
Gene
aac
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
May catalyze the coenzyme A-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides and confer resistance to aminoglycosides (By similarity). In vitro assays show no significant increase of resistance to aminoglycosides, possibly due to low expression in a heterologous system (PubMed:9159528).By similarity1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 35 | Substrate1 Publication | 1 | |
Binding sitei | 117 | Substrate; via carbonyl oxygen1 Publication | 1 |
GO - Molecular functioni
- aminoglycoside 2'-N-acetyltransferase activity Source: MTBBASE
- aminoglycoside N-acetyltransferase activity Source: MTBBASE
GO - Biological processi
- aminoglycoside antibiotic catabolic process Source: MTBBASE
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Antibiotic resistance |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4384-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Aminoglycoside 2'-N-acetyltransferase (EC:2.3.1.-)Alternative name(s): AAC(2')-Ic1 Publication |
Gene namesi | Name:aac Ordered Locus Names:Rv0262c ORF Names:MTCY06A4.06c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0262c |
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL3708053 |
DrugBanki | DB01842 3'-Phosphate-Adenosine-5'-Diphosphate DB01992 Coenzyme A DB01172 Kanamycin DB03615 Ribostamycin DB00684 Tobramycin |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064412 | 1 – 181 | Aminoglycoside 2'-N-acetyltransferaseAdd BLAST | 181 |
Proteomic databases
PaxDbi | P9WQG9 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv0262c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WQG9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 162 | N-acetyltransferasePROSITE-ProRule annotationAdd BLAST | 152 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 82 – 83 | Substrate binding | 2 | |
Regioni | 84 – 86 | Coenzyme A bindingCombined sources1 Publication | 3 | |
Regioni | 91 – 96 | Coenzyme A bindingCombined sources1 Publication | 6 | |
Regioni | 151 – 152 | Substrate binding1 Publication | 2 |
Sequence similaritiesi
Belongs to the AAC(2')-I acetyltransferase family.Curated
Phylogenomic databases
KOi | K17840 |
OMAi | VAVREDW |
Family and domain databases
InterProi | View protein in InterPro IPR016181 Acyl_CoA_acyltransferase IPR000182 GNAT_dom |
SUPFAMi | SSF55729 SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186 GNAT, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WQG9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHTQVHTARL VHTADLDSET RQDIRQMVTG AFAGDFTETD WEHTLGGMHA
60 70 80 90 100
LIWHHGAIIA HAAVIQRRLI YRGNALRCGY VEGVAVRADW RGQRLVSALL
110 120 130 140 150
DAVEQVMRGA YQLGALSSSA RARRLYASRG WLPWHGPTSV LAPTGPVRTP
160 170 180
DDDGTVFVLP IDISLDTSAE LMCDWRAGDV W
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72714 Genomic DNA Translation: AAB17563.1 AL123456 Genomic DNA Translation: CCP42991.1 |
PIRi | A70627 |
RefSeqi | NP_214776.1, NC_000962.3 WP_003899880.1, NZ_NVQJ01000055.1 |
Genome annotation databases
EnsemblBacteriai | CCP42991; CCP42991; Rv0262c |
GeneIDi | 886648 |
KEGGi | ag:AAB17563 mtu:Rv0262c mtv:RVBD_0262c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U72714 Genomic DNA Translation: AAB17563.1 AL123456 Genomic DNA Translation: CCP42991.1 |
PIRi | A70627 |
RefSeqi | NP_214776.1, NC_000962.3 WP_003899880.1, NZ_NVQJ01000055.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1M44 | X-ray | 1.60 | A/B | 1-181 | [»] | |
1M4D | X-ray | 1.80 | A/B | 1-181 | [»] | |
1M4G | X-ray | 1.80 | A/B | 1-181 | [»] | |
1M4I | X-ray | 1.50 | A/B | 1-181 | [»] | |
SMRi | P9WQG9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0262c |
Chemistry databases
ChEMBLi | CHEMBL3708053 |
DrugBanki | DB01842 3'-Phosphate-Adenosine-5'-Diphosphate DB01992 Coenzyme A DB01172 Kanamycin DB03615 Ribostamycin DB00684 Tobramycin |
Proteomic databases
PaxDbi | P9WQG9 |
Genome annotation databases
EnsemblBacteriai | CCP42991; CCP42991; Rv0262c |
GeneIDi | 886648 |
KEGGi | ag:AAB17563 mtu:Rv0262c mtv:RVBD_0262c |
Organism-specific databases
TubercuListi | Rv0262c |
Phylogenomic databases
KOi | K17840 |
OMAi | VAVREDW |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4384-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR016181 Acyl_CoA_acyltransferase IPR000182 GNAT_dom |
SUPFAMi | SSF55729 SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS51186 GNAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AAC2_MYCTU | |
Accessioni | P9WQG9Primary (citable) accession number: P9WQG9 Secondary accession number(s): L0T655 P95219 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 25 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references