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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA), Putative ATP-dependent 6-phosphofructokinase isozyme 2 (pfkB)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53Glyceraldehyde 3-phosphateBy similarity1
Active sitei95Proton donorBy similarity1
Metal bindingi96Zinc 1; catalyticBy similarity1
Metal bindingi131Zinc 2By similarity1
Metal bindingi161Zinc 2By similarity1
Metal bindingi212Zinc 1; catalyticBy similarity1
Binding sitei213Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi252Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00183

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Ordered Locus Names:MT0379
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004268111 – 344Fructose-bisphosphate aldolaseAdd BLAST344

Structurei

3D structure databases

ProteinModelPortaliP9WQA2
SMRiP9WQA2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni253 – 255Dihydroxyacetone phosphate bindingBy similarity3
Regioni274 – 277Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

KOiK01624
OrthoDBiPOG091H01YW

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000771 FBA_II
IPR006411 Fruct_bisP_bact
PANTHERiPTHR30559 PTHR30559, 1 hit
PfamiView protein in Pfam
PF01116 F_bP_aldolase, 1 hit
PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
TIGRFAMsiTIGR00167 cbbA, 1 hit
TIGR01520 FruBisAldo_II_A, 1 hit
PROSITEiView protein in PROSITE
PS00602 ALDOLASE_CLASS_II_1, 1 hit
PS00806 ALDOLASE_CLASS_II_2, 1 hit

Sequencei

Sequence statusi: Complete.

P9WQA2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPIATPEVYA EMLGQAKQNS YAFPAINCTS SETVNAAIKG FADAGSDGII
60 70 80 90 100
QFSTGGAEFG SGLGVKDMVT GAVALAEFTH VIAAKYPVNV ALHTDHCPKD
110 120 130 140 150
KLDSYVRPLL AISAQRVSKG GNPLFQSHMW DGSAVPIDEN LAIAQELLKA
160 170 180 190 200
AAAAKIILEI EIGVVGGEED GVANEINEKL YTSPEDFEKT IEALGAGEHG
210 220 230 240 250
KYLLAATFGN VHGVYKPGNV KLRPDILAQG QQVAAAKLGL PADAKPFDFV
260 270 280 290 300
FHGGSGSLKS EIEEALRYGV VKMNVDTDTQ YAFTRPIAGH MFTNYDGVLK
310 320 330 340
VDGEVGVKKV YDPRSYLKKA EASMSQRVVQ ACNDLHCAGK SLTH
Length:344
Mass (Da):36,544
Last modified:April 16, 2014 - v1
Checksum:i04FA3E1123F72FB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA Translation: AAK44600.1
PIRiD70576
RefSeqiWP_003401856.1, NZ_KK341227.1

Genome annotation databases

EnsemblBacteriaiAAK44600; AAK44600; MT0379
KEGGimtc:MT0379
PATRICifig|83331.31.peg.402

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA Translation: AAK44600.1
PIRiD70576
RefSeqiWP_003401856.1, NZ_KK341227.1

3D structure databases

ProteinModelPortaliP9WQA2
SMRiP9WQA2
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK44600; AAK44600; MT0379
KEGGimtc:MT0379
PATRICifig|83331.31.peg.402

Phylogenomic databases

KOiK01624
OrthoDBiPOG091H01YW

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00183

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR000771 FBA_II
IPR006411 Fruct_bisP_bact
PANTHERiPTHR30559 PTHR30559, 1 hit
PfamiView protein in Pfam
PF01116 F_bP_aldolase, 1 hit
PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
TIGRFAMsiTIGR00167 cbbA, 1 hit
TIGR01520 FruBisAldo_II_A, 1 hit
PROSITEiView protein in PROSITE
PS00602 ALDOLASE_CLASS_II_1, 1 hit
PS00806 ALDOLASE_CLASS_II_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiALF_MYCTO
AccessioniPrimary (citable) accession number: P9WQA2
Secondary accession number(s): L0T684, O06313, P67475
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: December 20, 2017
This is version 23 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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