The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P9WQ62 (MBTB_MYCTO)
Protein
Phenyloxazoline synthase MbtB
Gene
mbtB
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Functioni
Involved in the initial steps of the mycobactin biosynthetic pathway. Putatively couples activated salicylic acid with serine or threonine and cyclizes this precursor to the hydroxyphenyloxazoline ring system present in this class of siderophores. Essential for growth in macrophages.
1 PublicationCofactori
pantetheine 4'-phosphateBy similarityNote: Binds 2 phosphopantetheines covalently.By similarity
: mycobactin biosynthesis Pathwayi
This protein is involved in the pathway mycobactin biosynthesis, which is part of Siderophore biosynthesis.View all proteins of this organism that are known to be involved in the pathway mycobactin biosynthesis and in Siderophore biosynthesis.
GO - Molecular functioni
- ligase activity Source: UniProtKB-KW
- phosphopantetheine binding Source: InterPro
GO - Biological processi
- biosynthetic process Source: InterPro
Keywordsi
Molecular function | Ligase, Multifunctional enzyme |
Enzyme and pathway databases
UniPathwayi | UPA00011 |
Protein family/group databases
ESTHERi | myctu-MBTB, Thioesterase |
Names & Taxonomyi
Protein namesi | Recommended name: Phenyloxazoline synthase MbtB (EC:6.3.2.-)Alternative name(s): Mycobactin synthetase protein B |
Gene namesi | Name:mbtB Ordered Locus Names:MT2451 |
Organismi | Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
Taxonomic identifieri | 83331 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000426831 | 1 – 1414 | Phenyloxazoline synthase MbtBAdd BLAST | 1414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 39 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 1094 | O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication | 1 |
Post-translational modificationi
4'-phosphopantetheine is transferred from CoA to a specific serine in each of the two carrier protein domains, leading to their activation from apo to holo forms.
Keywords - PTMi
Phosphopantetheine, PhosphoproteinFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 78 | Carrier 1PROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 1057 – 1135 | Carrier 2PROSITE-ProRule annotationAdd BLAST | 79 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 96 – 394 | Condensation/cyclizationAdd BLAST | 299 | |
Regioni | 579 – 975 | AdenylationAdd BLAST | 397 | |
Regioni | 1188 – 1413 | ThioesteraseAdd BLAST | 226 |
Domaini
Modular protein that contains an aryl carrier protein (ArCP) domain which bears a phosphopantetheinyl arm to attach the activated salicylic acid, a condensation/cyclization domain involved in the formation of the oxazoline ring, an adenylation domain which activates the serine or threonine residue into an aminoacyl-AMP ester, a peptidyl carrier protein (PCP) domain which bears a phosphopantetheinyl arm to attach the activated serine or threonine, and a terminal thioesterase domain which assists in the transfer of intermediates from MbtB to ACP1 in MbtD.1 Publication
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | CLU_000022_2_4_11 |
Family and domain databases
Gene3Di | 1.10.1200.10, 2 hits 3.30.300.30, 1 hit 3.30.559.10, 1 hit 3.40.50.12780, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR001031, Thioesterase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit PF00668, Condensation, 1 hit PF00550, PP-binding, 2 hits PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00823, PKS_PP, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WQ62-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVHATACSEI IRAEVAELLG VRADALHPGA NLVGQGLDSI RMMSLVGRWR
60 70 80 90 100
RKGIAVDFAT LAATPTIEAW SQLVSAGTGV APTAVAAPGD AGLSQEGEPF
110 120 130 140 150
PVAPMQHAMW VGRHDHQQLG GVAGHLYVEF DGARVDPDRL RAAATRLALR
160 170 180 190 200
HPMLRVQFLP DGTQRIPPAA GSRDFPISVA DLRHVAPDVV DQRLAGIRDA
210 220 230 240 250
KSHQQLDGAV FELALTLLPG ERTRLHVDLD MQAADAMSYR ILLADLAALY
260 270 280 290 300
DGREPPALGY TYREYRQAIE AEETLPQPVR DADRDWWAQR IPQLPDPPAL
310 320 330 340 350
PTRAGGERDR RRSTRRWHWL DPQTRDALFA RARARGITPA MTLAAAFANV
360 370 380 390 400
LARWSASSRF LLNLPLFSRQ ALHPDVDLLV GDFTSSLLLD VDLTGARTAA
410 420 430 440 450
ARAQAVQEAL RTAAGHSAYP GLSVLRDLSR HRGTQVLAPV VFTSALGLGD
460 470 480 490 500
LFCPDVTEQF GTPGWIISQG PQVLLDAQVT EFDGGVLVNW DVREGVFAPG
510 520 530 540 550
VIDAMFTHQV DELLRLAAGD DAWDAPSPSA LPAAQRAVRA ALNGRTAAPS
560 570 580 590 600
TEALHDGFFR QAQQQPDAPA VFASSGDLSY AQLRDQASAV AAALRAAGLR
610 620 630 640 650
VGDTVAVLGP KTGEQVAAVL GILAAGGVYL PIGVDQPRDR AERILATGSV
660 670 680 690 700
NLALVCGPPC QVRVPVPTLL LADVLAAAPA EFVPGPSDPT ALAYVLFTSG
710 720 730 740 750
STGEPKGVEV AHDAAMNTVE TFIRHFELGA ADRWLALATL ECDMSVLDIF
760 770 780 790 800
AALRSGGAIV VVDEAQRRDP DAWARLIDTY EVTALNFMPG WLDMLLEVGG
810 820 830 840 850
GRLSSLRAVA VGGDWVRPDL ARRLQVQAPS ARFAGLGGAT ETAVHATIFE
860 870 880 890 900
VQDAANLPPD WASVPYGVPF PNNACRVVAD SGDDCPDWVA GELWVSGRGI
910 920 930 940 950
ARGYRGRPEL TAERFVEHDG RTWYRTGDLA RYWHDGTLEF VGRADHRVKI
960 970 980 990 1000
SGYRVELGEI EAALQRLPGV HAAAATVLPG GSDVLAAAVC VDDAGVTAES
1010 1020 1030 1040 1050
IRQQLADLVP AHMIPRHVTL LDRIPFTDSG KIDRAEVGAL LAAEVERSGD
1060 1070 1080 1090 1100
RSAPYAAPRT VLQRALRRIV ADILGRANDA VGVHDDFFAL GGDSVLATQV
1110 1120 1130 1140 1150
VAGIRRWLDS PSLMVADMFA ARTIAALAQL LTGREANADR LELVAEVYLE
1160 1170 1180 1190 1200
IANMTSADVM AALDPIEQPA QPAFKPWVKR FTGTDKPGAV LVFPHAGGAA
1210 1220 1230 1240 1250
AAYRWLAKSL VANDVDTFVV QYPQRADRRS HPAADSIEAL ALELFEAGDW
1260 1270 1280 1290 1300
HLTAPLTLFG HCMGAIVAFE FARLAERNGV PVRALWASSG QAPSTVAASG
1310 1320 1330 1340 1350
PLPTADRDVL ADMVDLGGTD PVLLEDEEFV ELLVPAVKAD YRALSGYSCP
1360 1370 1380 1390 1400
PDVRIRANIH AVGGNRDHRI SREMLTSWET HTSGRFTLSH FDGGHFYLND
1410
HLDAVARMVS ADVR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK46746.1 |
PIRi | B70674 |
RefSeqi | WP_010924520.1, NC_002755.2 |
Genome annotation databases
EnsemblBacteriai | AAK46746; AAK46746; MT2451 |
KEGGi | mtc:MT2451 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK46746.1 |
PIRi | B70674 |
RefSeqi | WP_010924520.1, NC_002755.2 |
3D structure databases
AlphaFoldDBi | P9WQ62 |
SMRi | P9WQ62 |
ModBasei | Search... |
Protein family/group databases
ESTHERi | myctu-MBTB, Thioesterase |
Genome annotation databases
EnsemblBacteriai | AAK46746; AAK46746; MT2451 |
KEGGi | mtc:MT2451 |
Phylogenomic databases
HOGENOMi | CLU_000022_2_4_11 |
Enzyme and pathway databases
UniPathwayi | UPA00011 |
Family and domain databases
Gene3Di | 1.10.1200.10, 2 hits 3.30.300.30, 1 hit 3.30.559.10, 1 hit 3.40.50.12780, 1 hit 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR010071, AA_adenyl_domain IPR029058, AB_hydrolase IPR036736, ACP-like_sf IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf IPR023213, CAT-like_dom_sf IPR001242, Condensatn IPR020806, PKS_PP-bd IPR009081, PP-bd_ACP IPR006162, Ppantetheine_attach_site IPR001031, Thioesterase |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit PF00668, Condensation, 1 hit PF00550, PP-binding, 2 hits PF00975, Thioesterase, 1 hit |
SMARTi | View protein in SMART SM00823, PKS_PP, 2 hits |
SUPFAMi | SSF47336, SSF47336, 2 hits SSF53474, SSF53474, 1 hit |
TIGRFAMsi | TIGR01733, AA-adenyl-dom, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit PS50075, CARRIER, 2 hits PS00012, PHOSPHOPANTETHEINE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MBTB_MYCTO | |
Accessioni | P9WQ62Primary (citable) accession number: P9WQ62 Secondary accession number(s): L0TCD0, P71717, Q7D788 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | May 25, 2022 | |
This is version 37 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families