UniProtKB - P9WPZ7 (ARGD_MYCTU)
Protein
Acetylornithine aminotransferase
Gene
argD
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Miscellaneous
May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.UniRule annotation
Was identified as a high-confidence drug target.
Catalytic activityi
- 2-oxoglutarate + N2-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehydeUniRule annotationEC:2.6.1.11UniRule annotation
Cofactori
pyridoxal 5'-phosphateUniRule annotationNote: Binds 1 pyridoxal phosphate per subunit.UniRule annotation
: L-arginine biosynthesis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Arginine biosynthesis bifunctional protein ArgJ (argJ), Amino-acid acetyltransferase (argA)
- Acetylglutamate kinase (argB)
- N-acetyl-gamma-glutamyl-phosphate reductase (argC)
- Acetylornithine aminotransferase (argD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 139 | Pyridoxal phosphate; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 142 | N2-acetyl-L-ornithineUniRule annotation | 1 | |
Binding sitei | 281 | N2-acetyl-L-ornithineUniRule annotation | 1 | |
Binding sitei | 282 | Pyridoxal phosphateUniRule annotation | 1 |
GO - Molecular functioni
- identical protein binding Source: GO_Central
- N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Source: UniProtKB-UniRule
- pyridoxal phosphate binding Source: GO_Central
GO - Biological processi
- arginine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00109 |
Names & Taxonomyi
Protein namesi | Recommended name: Acetylornithine aminotransferaseUniRule annotation (EC:2.6.1.11UniRule annotation)Short name: ACOATUniRule annotation |
Gene namesi | Name:argDUniRule annotation Ordered Locus Names:Rv1655 ORF Names:MTCY06H11.20 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1655 |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000112756 | 2 – 400 | Acetylornithine aminotransferaseAdd BLAST | 399 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonine; partialCombined sources1 Publication | 1 | |
Modified residuei | 253 | N6-(pyridoxal phosphate)lysineUniRule annotation | 1 | |
Cross-linki | 314 | Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication |
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | P9WPZ7 |
PRIDEi | P9WPZ7 |
PTM databases
iPTMneti | P9WPZ7 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 83332.Rv1655 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 113 – 114 | Pyridoxal phosphate bindingUniRule annotation | 2 | |
Regioni | 224 – 227 | Pyridoxal phosphate bindingUniRule annotation | 4 |
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG4992, Bacteria |
OMAi | GWGLING |
PhylomeDBi | P9WPZ7 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01107, ArgD_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR004636, AcOrn/SuccOrn_fam IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00707, argD, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P9WPZ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTGASTTTAT MRQRWQAVMM NNYGTPPIAL ASGDGAVVTD VDGRTYIDLL
60 70 80 90 100
GGIAVNVLGH RHPAVIEAVT RQMSTLGHTS NLYATEPGIA LAEELVALLG
110 120 130 140 150
ADQRTRVFFC NSGAEANEAA FKLSRLTGRT KLVAAHDAFH GRTMGSLALT
160 170 180 190 200
GQPAKQTPFA PLPGDVTHVG YGDVDALAAA VDDHTAAVFL EPIMGESGVV
210 220 230 240 250
VPPAGYLAAA RDITARRGAL LVLDEVQTGM GRTGAFFAHQ HDGITPDVVT
260 270 280 290 300
LAKGLGGGLP IGACLAVGPA AELLTPGLHG STFGGNPVCA AAALAVLRVL
310 320 330 340 350
ASDGLVRRAE VLGKSLRHGI EALGHPLIDH VRGRGLLLGI ALTAPHAKDA
360 370 380 390 400
EATARDAGYL VNAAAPDVIR LAPPLIIAEA QLDGFVAALP AILDRAVGAP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44420.1 |
PIRi | B70621 |
RefSeqi | NP_216171.1, NC_000962.3 WP_003408163.1, NZ_NVQJ01000069.1 |
Genome annotation databases
EnsemblBacteriai | CCP44420; CCP44420; Rv1655 |
GeneIDi | 23492179 885187 |
KEGGi | mtu:Rv1655 mtv:RVBD_1655 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44420.1 |
PIRi | B70621 |
RefSeqi | NP_216171.1, NC_000962.3 WP_003408163.1, NZ_NVQJ01000069.1 |
3D structure databases
SMRi | P9WPZ7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1655 |
PTM databases
iPTMneti | P9WPZ7 |
Proteomic databases
PaxDbi | P9WPZ7 |
PRIDEi | P9WPZ7 |
Genome annotation databases
EnsemblBacteriai | CCP44420; CCP44420; Rv1655 |
GeneIDi | 23492179 885187 |
KEGGi | mtu:Rv1655 mtv:RVBD_1655 |
Organism-specific databases
TubercuListi | Rv1655 |
Phylogenomic databases
eggNOGi | COG4992, Bacteria |
OMAi | GWGLING |
PhylomeDBi | P9WPZ7 |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00109 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_01107, ArgD_aminotrans_3, 1 hit |
InterProi | View protein in InterPro IPR004636, AcOrn/SuccOrn_fam IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00707, argD, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARGD_MYCTU | |
Accessioni | P9WPZ7Primary (citable) accession number: P9WPZ7 Secondary accession number(s): L0T8W6, P63568, P94990 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 43 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families