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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate (Probable). Can use both NADH and NADPH as a reductant, with NADH being 6-fold as effective as NADPH.UniRule annotationCurated1 Publication

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.Curated

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotation

Kineticsi

  1. KM=3.2 µM for NADH1 Publication
  2. KM=11.8 µM for NADPH1 Publication

    Pathwayi: L-lysine biosynthesis via DAP pathway

    This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
    Proteins known to be involved in the 4 steps of the subpathway in this organism are:
    1. Aspartokinase (ask), Aspartokinase (LH57_20215)
    2. Aspartate-semialdehyde dehydrogenase (asd), Aspartate-semialdehyde dehydrogenase (asd)
    3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA), 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
    4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB), 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
    This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei33NADUniRule annotation1 Publication1
    Active sitei132Proton donor/acceptorUniRule annotation1
    Binding sitei133Substrate1
    Active sitei136Proton donorCurated1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi7 – 12NAD(P)UniRule annotation1 Publication6
    Nucleotide bindingi75 – 77NAD(P)UniRule annotation1 Publication3
    Nucleotide bindingi102 – 105NAD(P)UniRule annotation1 Publication4

    GO - Molecular functioni

    • 4-hydroxy-tetrahydrodipicolinate reductase Source: MTBBASE
    • NADH binding Source: MTBBASE
    • NADPH binding Source: MTBBASE

    GO - Biological processi

    • diaminopimelate biosynthetic process Source: MTBBASE
    • lysine biosynthetic process via diaminopimelate Source: MTBBASE

    Keywordsi

    Molecular functionOxidoreductase
    Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
    LigandNAD, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:G185E-7022-MONOMER
    SABIO-RKiP9WP23
    UniPathwayiUPA00034; UER00018

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotation (EC:1.17.1.8UniRule annotation)
    Short name:
    HTPA reductaseUniRule annotation
    Gene namesi
    Name:dapBUniRule annotation
    Ordered Locus Names:Rv2773c
    ORF Names:MTV002.38c
    OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    Taxonomic identifieri83332 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    Proteomesi
    • UP000001584 Componenti: Chromosome

    Organism-specific databases

    TubercuListiRv2773c

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    • cell wall Source: MTBBASE
    • cytosol Source: GO_Central
    • plasma membrane Source: MTBBASE

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi9K → A: Increases the nucleotide specificity from 6:1 for the wild-type enzyme to 34:1, due to a 4-fold decrease in NADPH affinity while the affinity for NADH remains nearly unchanged. 1 Publication1
    Mutagenesisi11K → A: 2.8-fold increase in catalytic activity with NADH as substrate, while the affinity for NADH is essentially unaffected. 70-fold decrease in affinity for NADPH, causing the nucleotide specificity to increase from 6:1 for the wild-type enzyme to 187:1. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001414581 – 2454-hydroxy-tetrahydrodipicolinate reductaseAdd BLAST245

    Proteomic databases

    PaxDbiP9WP23

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation3 Publications

    Protein-protein interaction databases

    STRINGi83332.Rv2773c

    Structurei

    Secondary structure

    1245
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Turni7 – 9Combined sources3
    Helixi11 – 22Combined sources12
    Beta strandi27 – 32Combined sources6
    Beta strandi34 – 36Combined sources3
    Helixi39 – 43Combined sources5
    Beta strandi48 – 51Combined sources4
    Turni55 – 57Combined sources3
    Helixi58 – 67Combined sources10
    Beta strandi71 – 74Combined sources4
    Helixi81 – 92Combined sources12
    Beta strandi98 – 101Combined sources4
    Helixi107 – 119Combined sources13
    Helixi120 – 122Combined sources3
    Beta strandi124 – 132Combined sources9
    Beta strandi138 – 140Combined sources3
    Helixi142 – 154Combined sources13
    Turni155 – 157Combined sources3
    Turni170 – 173Combined sources4
    Beta strandi175 – 177Combined sources3
    Beta strandi180 – 186Combined sources7
    Beta strandi192 – 200Combined sources9
    Beta strandi203 – 211Combined sources9
    Helixi214 – 217Combined sources4
    Helixi218 – 227Combined sources10
    Helixi228 – 230Combined sources3
    Beta strandi233 – 238Combined sources6
    Helixi239 – 242Combined sources4

    3D structure databases

    ProteinModelPortaliP9WP23
    SMRiP9WP23
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni142 – 143Substrate binding2

    Sequence similaritiesi

    Belongs to the DapB family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105DUK Bacteria
    COG0289 LUCA
    KOiK00215
    OMAiRESFMPG
    PhylomeDBiP9WP23

    Family and domain databases

    HAMAPiMF_00102 DapB, 1 hit
    InterProiView protein in InterPro
    IPR022663 DapB_C
    IPR000846 DapB_N
    IPR022664 DapB_N_CS
    IPR023940 DHDPR_bac
    IPR036291 NAD(P)-bd_dom_sf
    PANTHERiPTHR20836 PTHR20836, 1 hit
    PfamiView protein in Pfam
    PF05173 DapB_C, 1 hit
    PF01113 DapB_N, 1 hit
    PIRSFiPIRSF000161 DHPR, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR00036 dapB, 1 hit
    PROSITEiView protein in PROSITE
    PS01298 DAPB, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P9WP23-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRVGVLGAKG KVGATMVRAV AAADDLTLSA ELDAGDPLSL LTDGNTEVVI
    60 70 80 90 100
    DFTHPDVVMG NLEFLIDNGI HAVVGTTGFT AERFQQVESW LVAKPNTSVL
    110 120 130 140 150
    IAPNFAIGAV LSMHFAKQAA RFFDSAEVIE LHHPHKADAP SGTAARTAKL
    160 170 180 190 200
    IAEARKGLPP NPDATSTSLP GARGADVDGI PVHAVRLAGL VAHQEVLFGT
    210 220 230 240
    EGETLTIRHD SLDRTSFVPG VLLAVRRIAE RPGLTVGLEP LLDLH
    Length:245
    Mass (Da):25,733
    Last modified:April 16, 2014 - v1
    Checksum:iCDB8273AFFD4C095
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti14A → T in AAC45142 (PubMed:9098082).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U66101 Genomic DNA Translation: AAC45142.1
    AL123456 Genomic DNA Translation: CCP45572.1
    PIRiD70882
    RefSeqiNP_217289.1, NC_000962.3
    WP_003414099.1, NZ_KK339370.1

    Genome annotation databases

    EnsemblBacteriaiCCP45572; CCP45572; Rv2773c
    GeneIDi888443
    KEGGimtu:Rv2773c

    Similar proteinsi

    Entry informationi

    Entry nameiDAPB_MYCTU
    AccessioniPrimary (citable) accession number: P9WP23
    Secondary accession number(s): L0TDI3, O33315, P72024
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: April 25, 2018
    This is version 29 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

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