UniProtKB - P9WP01 (PUNA_MYCTU)
Protein
Purine nucleoside phosphorylase
Gene
punA
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine, inosine, 2'-deoxyguanosine and 2'-deoxyinosine.By similarity
Catalytic activityi
- a purine 2'-deoxy-D-ribonucleoside + phosphate = 2-deoxy-α-D-ribose 1-phosphate + a purine nucleobaseBy similarityEC:2.4.2.1By similarity
: purine nucleoside salvage Pathwayi
This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 36 | PhosphateCombined sources1 Publication | 1 | |
Binding sitei | 68 | PhosphateBy similarity | 1 | |
Binding sitei | 120 | Phosphate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 189 | Purine nucleoside1 Publication | 1 | |
Binding sitei | 208 | PhosphateCombined sources1 Publication | 1 | |
Binding sitei | 231 | Purine nucleoside1 Publication | 1 |
GO - Molecular functioni
- purine-nucleoside phosphorylase activity Source: MTBBASE
GO - Biological processi
- deoxyguanosine catabolic process Source: MTBBASE
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7581-MONOMER |
UniPathwayi | UPA00606 |
Names & Taxonomyi
Protein namesi | Recommended name: Purine nucleoside phosphorylase (EC:2.4.2.1)Short name: PNP Short name: Pu-NPase Alternative name(s): Inosine phosphorylase Inosine-guanosine phosphorylase |
Gene namesi | Name:punA Synonyms:deoD Ordered Locus Names:Rv3307 ORF Names:MTV016.06 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3307 |
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL1169594 |
DrugBanki | DB03551 (3R,4R)-3-Hydroxy-4-(hydroxymethyl)-1-[(4-oxo-4,4a,5,7a-tetrahydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]pyrrolidinium DB03411 2-Hydroxymethyl-Pyrrolidine-3,4-Diol DB04095 9-Deazahypoxanthine |
DrugCentrali | P9WP01 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184544 | 1 – 268 | Purine nucleoside phosphorylaseAdd BLAST | 268 |
Proteomic databases
PaxDbi | P9WP01 |
Interactioni
Subunit structurei
Homotrimer.
By similarityProtein-protein interaction databases
STRINGi | 83332.Rv3307 |
Chemistry databases
BindingDBi | P9WP01 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WP01 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 88 – 90 | Phosphate bindingCombined sources1 Publication | 3 |
Sequence similaritiesi
Belongs to the PNP/MTAP phosphorylase family.Curated
Phylogenomic databases
eggNOGi | ENOG4107QT0 Bacteria COG0005 LUCA |
KOi | K03783 |
OMAi | EGVYAQF |
PhylomeDBi | P9WP01 |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR000845 Nucleoside_phosphorylase_d IPR035994 Nucleoside_phosphorylase_sf IPR011269 PUNP IPR011268 Purine_phosphorylase IPR018099 Purine_phosphorylase-2_CS |
PANTHERi | PTHR11904 PTHR11904, 1 hit |
Pfami | View protein in Pfam PF01048 PNP_UDP_1, 1 hit |
PIRSFi | PIRSF000477 PurNPase, 1 hit |
SUPFAMi | SSF53167 SSF53167, 1 hit |
TIGRFAMsi | TIGR01697 PNPH-PUNA-XAPA, 1 hit TIGR01698 PUNP, 1 hit |
PROSITEi | View protein in PROSITE PS01240 PNP_MTAP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WP01-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADPRPDPDE LARRAAQVIA DRTGIGEHDV AVVLGSGWLP AVAALGSPTT
60 70 80 90 100
VLPQAELPGF VPPTAAGHAG ELLSVPIGAH RVLVLAGRIH AYEGHDLRYV
110 120 130 140 150
VHPVRAARAA GAQIMVLTNA AGGLRADLQV GQPVLISDHL NLTARSPLVG
160 170 180 190 200
GEFVDLTDAY SPRLRELARQ SDPQLAEGVY AGLPGPHYET PAEIRMLQTL
210 220 230 240 250
GADLVGMSTV HETIAARAAG AEVLGVSLVT NLAAGITGEP LSHAEVLAAG
260
AASATRMGAL LADVIARF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46126.1 |
PIRi | B70842 |
RefSeqi | NP_217824.1, NC_000962.3 WP_003417233.1, NZ_NVQJ01000003.1 |
Genome annotation databases
EnsemblBacteriai | CCP46126; CCP46126; Rv3307 |
GeneIDi | 887542 |
KEGGi | mtu:Rv3307 mtv:RVBD_3307 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46126.1 |
PIRi | B70842 |
RefSeqi | NP_217824.1, NC_000962.3 WP_003417233.1, NZ_NVQJ01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G2O | X-ray | 1.75 | A/B/C | 1-268 | [»] | |
1I80 | X-ray | 2.00 | A/B/C | 1-268 | [»] | |
1N3I | X-ray | 1.90 | A/B/C | 1-268 | [»] | |
3IOM | X-ray | 2.14 | A/B | 1-268 | [»] | |
3SCZ | X-ray | 1.95 | A/B | 1-268 | [»] | |
SMRi | P9WP01 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3307 |
Chemistry databases
BindingDBi | P9WP01 |
ChEMBLi | CHEMBL1169594 |
DrugBanki | DB03551 (3R,4R)-3-Hydroxy-4-(hydroxymethyl)-1-[(4-oxo-4,4a,5,7a-tetrahydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]pyrrolidinium DB03411 2-Hydroxymethyl-Pyrrolidine-3,4-Diol DB04095 9-Deazahypoxanthine |
DrugCentrali | P9WP01 |
Proteomic databases
PaxDbi | P9WP01 |
Genome annotation databases
EnsemblBacteriai | CCP46126; CCP46126; Rv3307 |
GeneIDi | 887542 |
KEGGi | mtu:Rv3307 mtv:RVBD_3307 |
Organism-specific databases
TubercuListi | Rv3307 |
Phylogenomic databases
eggNOGi | ENOG4107QT0 Bacteria COG0005 LUCA |
KOi | K03783 |
OMAi | EGVYAQF |
PhylomeDBi | P9WP01 |
Enzyme and pathway databases
UniPathwayi | UPA00606 |
BioCyci | MTBH37RV:G185E-7581-MONOMER |
Miscellaneous databases
PROi | PR:P9WP01 |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR000845 Nucleoside_phosphorylase_d IPR035994 Nucleoside_phosphorylase_sf IPR011269 PUNP IPR011268 Purine_phosphorylase IPR018099 Purine_phosphorylase-2_CS |
PANTHERi | PTHR11904 PTHR11904, 1 hit |
Pfami | View protein in Pfam PF01048 PNP_UDP_1, 1 hit |
PIRSFi | PIRSF000477 PurNPase, 1 hit |
SUPFAMi | SSF53167 SSF53167, 1 hit |
TIGRFAMsi | TIGR01697 PNPH-PUNA-XAPA, 1 hit TIGR01698 PUNP, 1 hit |
PROSITEi | View protein in PROSITE PS01240 PNP_MTAP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUNA_MYCTU | |
Accessioni | P9WP01Primary (citable) accession number: P9WP01 Secondary accession number(s): L0TDR7, O53359, P0A538 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 42 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references