UniProtKB - P9WNW7 (HSAC_MYCTU)
Protein
Iron-dependent extradiol dioxygenase
Gene
hsaC
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oic acid (4,9-DSHA).1 Publication
Miscellaneous
Cholesterol metabolism contributes to the survival of M.tuberculosis in the host by helping the bacterial multiplication during earlier stages of infection and to the dissemination of the pathogen in the host.
Catalytic activityi
- 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = (1E,2Z)-3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate + H+1 PublicationEC:1.13.11.251 Publication
Cofactori
Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication
Kineticsi
- KM=0.9 µM for 3,4-DHSA (at pH 8 and at 25 degrees Celsius)1 Publication
- KM=8.5 µM for 2,3-dihydroxybiphenyl (DHB) (at pH 8 and at 25 degrees Celsius)1 Publication
- Vmax=12 µmol/sec/mg enzyme with 3,4-DHSA as substrate (at pH 8 and at 25 degrees Celsius)1 Publication
- Vmax=2.5 µmol/sec/mg enzyme with DHB as substrate (at pH 8 and at 25 degrees Celsius)1 Publication
: cholesterol metabolism Pathwayi
This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.View all proteins of this organism that are known to be involved in the pathway cholesterol metabolism and in Steroid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 145 | Iron | 1 | |
Binding sitei | 200 | Substrate1 Publication | 1 | |
Metal bindingi | 215 | Iron | 1 | |
Binding sitei | 215 | Substrate1 Publication | 1 | |
Binding sitei | 250 | Substrate1 Publication | 1 | |
Binding sitei | 256 | Substrate1 Publication | 1 | |
Metal bindingi | 266 | Iron | 1 |
GO - Molecular functioni
- 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase activity Source: MTBBASE
- ferrous iron binding Source: InterPro
- iron ion binding Source: MTBBASE
GO - Biological processi
- aromatic compound catabolic process Source: UniProtKB-KW
- cholesterol catabolic process Source: MTBBASE
- pathogenesis Source: MTBBASE
- response to cholesterol Source: MTBBASE
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Aromatic hydrocarbons catabolism, Cholesterol metabolism, Lipid degradation, Lipid metabolism, Steroid metabolism, Sterol metabolism |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:G185E-7846-MONOMER |
SABIO-RKi | P9WNW7 |
UniPathwayi | UPA00296 |
Chemistry databases
SwissLipidsi | SLP:000001005 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:hsaC Synonyms:bphC Ordered Locus Names:Rv3568c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3568c |
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene completely fail to grow on cholesterol due the blockage of the catabolic pathway and develop a pink color consistent with the accumulation of toxic catechols. Mice intravenously infected with the mutant survive substantially longer than those infected with the wild-type.1 Publication
Chemistry databases
DrugBanki | DB08542, 3,4-dihydroxy-9,10-secoandrosta-1(10),2,4-triene-9,17-dione |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000404507 | 1 – 300 | Iron-dependent extradiol dioxygenaseAdd BLAST | 300 |
Proteomic databases
PaxDbi | P9WNW7 |
Expressioni
Inductioni
Induced by KstR.1 Publication
Interactioni
Subunit structurei
Homodimer, but may form a homooctamer.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv3568c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WNW7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 120 | VOC 1PROSITE-ProRule annotationAdd BLAST | 116 | |
Domaini | 142 – 270 | VOC 2PROSITE-ProRule annotationAdd BLAST | 129 |
Sequence similaritiesi
Belongs to the extradiol ring-cleavage dioxygenase family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG0346, Bacteria |
OMAi | KMSATLG |
PhylomeDBi | P9WNW7 |
Family and domain databases
Gene3Di | 3.10.180.10, 2 hits |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR037523, VOC IPR000486, Xdiol_ring_cleave_dOase_1/2 |
Pfami | View protein in Pfam PF00903, Glyoxalase, 1 hit |
SUPFAMi | SSF54593, SSF54593, 2 hits |
PROSITEi | View protein in PROSITE PS00082, EXTRADIOL_DIOXYGENAS, 1 hit PS51819, VOC, 2 hits |
i Sequence
Sequence statusi: Complete.
P9WNW7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIRSLGYLR IEATDMAAWR EYGLKVLGMV EGKGAPEGAL YLRMDDFPAR
60 70 80 90 100
LVVVPGEHDR LLEAGWECAN AEGLQEIRNR LDLEGTPYKE ATAAELADRR
110 120 130 140 150
VDEMIRFADP SGNCLEVFHG TALEHRRVVS PYGHRFVTGE QGMGHVVLST
160 170 180 190 200
RDDAEALHFY RDVLGFRLRD SMRLPPQMVG RPADGPPAWL RFFGCNPRHH
210 220 230 240 250
SLAFLPMPTS SGIVHLMVEV EQADDVGLCL DRALRRKVPM SATLGRHVND
260 270 280 290 300
LMLSFYMKTP GGFDIEFGCE GRQVDDRDWI ARESTAVSLW GHDFTVGARG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46391.1 |
PIRi | F70605 |
RefSeqi | NP_218085.1, NC_000962.3 WP_003419378.1, NZ_NVQJ01000014.1 |
Genome annotation databases
EnsemblBacteriai | CCP46391; CCP46391; Rv3568c |
GeneIDi | 23490157 887886 |
KEGGi | mtu:Rv3568c mtv:RVBD_3568c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46391.1 |
PIRi | F70605 |
RefSeqi | NP_218085.1, NC_000962.3 WP_003419378.1, NZ_NVQJ01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ZI8 | X-ray | 2.20 | A/B | 1-300 | [»] | |
2ZYQ | X-ray | 2.00 | A/B | 1-300 | [»] | |
SMRi | P9WNW7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3568c |
Chemistry databases
DrugBanki | DB08542, 3,4-dihydroxy-9,10-secoandrosta-1(10),2,4-triene-9,17-dione |
SwissLipidsi | SLP:000001005 |
Proteomic databases
PaxDbi | P9WNW7 |
Genome annotation databases
EnsemblBacteriai | CCP46391; CCP46391; Rv3568c |
GeneIDi | 23490157 887886 |
KEGGi | mtu:Rv3568c mtv:RVBD_3568c |
Organism-specific databases
TubercuListi | Rv3568c |
Phylogenomic databases
eggNOGi | COG0346, Bacteria |
OMAi | KMSATLG |
PhylomeDBi | P9WNW7 |
Enzyme and pathway databases
UniPathwayi | UPA00296 |
BioCyci | MetaCyc:G185E-7846-MONOMER |
SABIO-RKi | P9WNW7 |
Family and domain databases
Gene3Di | 3.10.180.10, 2 hits |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR037523, VOC IPR000486, Xdiol_ring_cleave_dOase_1/2 |
Pfami | View protein in Pfam PF00903, Glyoxalase, 1 hit |
SUPFAMi | SSF54593, SSF54593, 2 hits |
PROSITEi | View protein in PROSITE PS00082, EXTRADIOL_DIOXYGENAS, 1 hit PS51819, VOC, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HSAC_MYCTU | |
Accessioni | P9WNW7Primary (citable) accession number: P9WNW7 Secondary accession number(s): L0TFW3, P96850, Q7D597 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 33 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families