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Entry version 32 (18 Sep 2019)
Sequence version 1 (16 Apr 2014)
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Protein

DNA primase

Gene

dnaG

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.UniRule annotation

Miscellaneous

Was identified as a high-confidence drug target.

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi268Magnesium 1; catalyticUniRule annotation1
Metal bindingi319Magnesium 1; catalyticUniRule annotation1
Metal bindingi319Magnesium 2UniRule annotation1
Metal bindingi321Magnesium 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri41 – 65CHC2-typeUniRule annotationAdd BLAST25

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MTBH37RV:G185E-6569-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA primaseUniRule annotation (EC:2.7.7.-UniRule annotation)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:dnaGUniRule annotation
Ordered Locus Names:Rv2343c
ORF Names:MTCY98.12c
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83332 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

Organism-specific databases

Mycobacterium tuberculosis strain H37Rv genome database

More...
TubercuListi
Rv2343c

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001805061 – 639DNA primaseAdd BLAST639

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P9WNW1

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

Interacts with DnaB.

UniRule annotation

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
83332.Rv2343c

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1639
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P9WNW1

Database of comparative protein structure models

More...
ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

More...
PDBe-KBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini262 – 348ToprimUniRule annotationAdd BLAST87

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Contains an N-terminal zinc-binding domain, a central core domain that contains the primase activity, and a C-terminal DnaB-binding domain.UniRule annotation

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the DnaG primase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 65CHC2-typeUniRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105C9G Bacteria
COG0358 LUCA

KEGG Orthology (KO)

More...
KOi
K02316

Identification of Orthologs from Complete Genome Data

More...
OMAi
RIMFPIY

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P9WNW1

Family and domain databases

Conserved Domains Database

More...
CDDi
cd03364 TOPRIM_DnaG_primases, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.90.580.10, 1 hit
3.90.980.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00974 DNA_primase_DnaG, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013264 DNA_primase_core_N
IPR037068 DNA_primase_core_N_sf
IPR019475 DNA_primase_DnaB-bd
IPR006295 DNA_primase_DnaG
IPR013173 DNA_primase_DnaG_DnaB-bd_dom
IPR036977 DNA_primase_Znf_CHC2
IPR030846 DnaG_bac
IPR034151 TOPRIM_DnaG_bac
IPR006171 TOPRIM_domain
IPR002694 Znf_CHC2

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF10410 DnaB_bind, 1 hit
PF08278 DnaG_DnaB_bind, 1 hit
PF13662 Toprim_4, 1 hit
PF08275 Toprim_N, 1 hit
PF01807 zf-CHC2, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF002811 DnaG, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00766 DnaG_DnaB_bind, 1 hit
SM00493 TOPRIM, 1 hit
SM00400 ZnF_CHCC, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01391 dnaG, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50880 TOPRIM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P9WNW1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGRISDRDI AAIREGARIE DVVGDYVQLR RAGADSLKGL CPFHNEKSPS
60 70 80 90 100
FHVRPNHGHF HCFGCGEGGD VYAFIQKIEH VSFVEAVELL ADRIGHTISY
110 120 130 140 150
TGAATSVQRD RGSRSRLLAA NAAAAAFYAQ ALQSDEAAPA RQYLTERSFD
160 170 180 190 200
AAAARKFGCG FAPSGWDSLT KHLQRKGFEF EELEAAGLSR QGRHGPMDRF
210 220 230 240 250
HRRLLWPIRT SAGEVVGFGA RRLFDDDAME AKYVNTPETL LYKKSSVMFG
260 270 280 290 300
IDLAKRDIAK GHQAVVVEGY TDVMAMHLAG VTTAVASCGT AFGGEHLAML
310 320 330 340 350
RRLMMDDSFF RGELIYVFDG DEAGRAAALK AFDGEQKLAG QSFVAVAPDG
360 370 380 390 400
MDPCDLRLKC GDAALRDLVA RRTPLFEFAI RAAIAEMDLD SAEGRVAALR
410 420 430 440 450
RCVPMVGQIK DPTLRDEYAR QLAGWVGWAD VAQVIGRVRG EAKRTKHPRL
460 470 480 490 500
GRLGSTTIAR AAQRPTAGPP TELAVRPDPR DPTLWPQREA LKSALQYPAL
510 520 530 540 550
AGPVFDALTV EGFTHPEYAA VRAAIDTAGG TSAGLSGAQW LDMVRQQTTS
560 570 580 590 600
TVTSALISEL GVEAIQVDDD KLPRYIAGVL ARLQEVWLGR QIAEVKSKLQ
610 620 630
RMSPIEQGDE YHALFGDLVA MEAYRRSLLE QASGDDLTA
Length:639
Mass (Da):69,593
Last modified:April 16, 2014 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE5A4887F2357C833
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45131.1

Protein sequence database of the Protein Information Resource

More...
PIRi
H70661

NCBI Reference Sequences

More...
RefSeqi
NP_216859.1, NC_000962.3
WP_003412049.1, NZ_NVQJ01000012.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CCP45131; CCP45131; Rv2343c

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
885996

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mtu:Rv2343c
mtv:RVBD_2343c

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45131.1
PIRiH70661
RefSeqiNP_216859.1, NC_000962.3
WP_003412049.1, NZ_NVQJ01000012.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5W33X-ray2.85A112-432[»]
5W34X-ray2.95A/B112-432[»]
5W35X-ray3.31A/B112-432[»]
5W36X-ray2.46A/B112-432[»]
SMRiP9WNW1
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2343c

Proteomic databases

PaxDbiP9WNW1

Genome annotation databases

EnsemblBacteriaiCCP45131; CCP45131; Rv2343c
GeneIDi885996
KEGGimtu:Rv2343c
mtv:RVBD_2343c

Organism-specific databases

TubercuListiRv2343c

Phylogenomic databases

eggNOGiENOG4105C9G Bacteria
COG0358 LUCA
KOiK02316
OMAiRIMFPIY
PhylomeDBiP9WNW1

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-6569-MONOMER

Family and domain databases

CDDicd03364 TOPRIM_DnaG_primases, 1 hit
Gene3Di3.90.580.10, 1 hit
3.90.980.10, 1 hit
HAMAPiMF_00974 DNA_primase_DnaG, 1 hit
InterProiView protein in InterPro
IPR013264 DNA_primase_core_N
IPR037068 DNA_primase_core_N_sf
IPR019475 DNA_primase_DnaB-bd
IPR006295 DNA_primase_DnaG
IPR013173 DNA_primase_DnaG_DnaB-bd_dom
IPR036977 DNA_primase_Znf_CHC2
IPR030846 DnaG_bac
IPR034151 TOPRIM_DnaG_bac
IPR006171 TOPRIM_domain
IPR002694 Znf_CHC2
PfamiView protein in Pfam
PF10410 DnaB_bind, 1 hit
PF08278 DnaG_DnaB_bind, 1 hit
PF13662 Toprim_4, 1 hit
PF08275 Toprim_N, 1 hit
PF01807 zf-CHC2, 1 hit
PIRSFiPIRSF002811 DnaG, 1 hit
SMARTiView protein in SMART
SM00766 DnaG_DnaB_bind, 1 hit
SM00493 TOPRIM, 1 hit
SM00400 ZnF_CHCC, 1 hit
TIGRFAMsiTIGR01391 dnaG, 1 hit
PROSITEiView protein in PROSITE
PS50880 TOPRIM, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDNAG_MYCTU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P9WNW1
Secondary accession number(s): L0TC89, P63962, P95239
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: September 18, 2019
This is version 32 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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