UniProtKB - P9WNV6 (DNAJ2_MYCTO)
Protein
Chaperone protein DnaJ 2
Gene
dnaJ2
Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Functioni
Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation
Cofactori
Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 145 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 148 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 162 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 165 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 188 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 191 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 202 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 205 | Zinc 1UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 132 – 214 | CR-typeUniRule annotationAdd BLAST | 83 |
GO - Molecular functioni
- ATP binding Source: InterPro
- heat shock protein binding Source: InterPro
- unfolded protein binding Source: UniProtKB-UniRule
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- DNA replication Source: UniProtKB-KW
- protein folding Source: InterPro
- response to heat Source: InterPro
Keywordsi
Molecular function | Chaperone |
Biological process | DNA replication, Stress response |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Chaperone protein DnaJ 2UniRule annotation |
Gene namesi | Name:dnaJ2UniRule annotation Ordered Locus Names:MT2442 |
Organismi | Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) |
Taxonomic identifieri | 83331 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000427060 | 1 – 382 | Chaperone protein DnaJ 2Add BLAST | 382 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationGO - Molecular functioni
- heat shock protein binding Source: InterPro
- unfolded protein binding Source: UniProtKB-UniRule
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 68 | JUniRule annotationAdd BLAST | 65 | |
Repeati | 145 – 152 | CXXCXGXG motif | 8 | |
Repeati | 162 – 169 | CXXCXGXG motif | 8 | |
Repeati | 188 – 195 | CXXCXGXG motif | 8 | |
Repeati | 202 – 209 | CXXCXGXG motif | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 75 – 115 | Gly-richAdd BLAST | 41 |
Domaini
The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation
Sequence similaritiesi
Belongs to the DnaJ family.UniRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 132 – 214 | CR-typeUniRule annotationAdd BLAST | 83 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
HOGENOMi | CLU_017633_0_7_11 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit cd10719, DnaJ_zf, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
HAMAPi | MF_01152, DnaJ, 1 hit |
InterProi | View protein in InterPro IPR012724, DnaJ IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR008971, HSP40/DnaJ_pept-bd IPR001305, HSP_DnaJ_Cys-rich_dom IPR036410, HSP_DnaJ_Cys-rich_dom_sf IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit PF00684, DnaJ_CXXCXGXG, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits SSF57938, SSF57938, 1 hit |
PROSITEi | View protein in PROSITE PS50076, DNAJ_2, 1 hit PS51188, ZF_CR, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WNV6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARDYYGLLG VSKNASDADI KRAYRKLARE LHPDVNPDEA AQAKFKEISV
60 70 80 90 100
AYEVLSDPDK RRIVDLGGDP LESAAAGGNG FGGFGGLGDV FEAFFGGGFG
110 120 130 140 150
GGAASRGPIG RVRPGSDSLL RMRLDLEECA TGVTKQVTVD TAVLCDRCQG
160 170 180 190 200
KGTNGDSVPI PCDTCGGRGE VQTVQRSLLG QMLTSRPCPT CRGVGVVIPD
210 220 230 240 250
PCQQCMGDGR IRARREISVK IPAGVGDGMR VRLAAQGEVG PGGGPAGDLY
260 270 280 290 300
VEVHEQAHDV FVREGDHLHC TVSVPMVDAA LGVTVTVDAI LDGLSEITIP
310 320 330 340 350
PGTQPGSVIT LRGRGMPHLR SNTRGDLHVH VEVVVPTRLD HQDIELLREL
360 370 380
KGRRDREVAE VRSTHAAAGG LFSRLRETFT GR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK46736.1 |
PIRi | D70587 |
RefSeqi | WP_003899294.1, NZ_KK341227.1 |
Genome annotation databases
EnsemblBacteriai | AAK46736; AAK46736; MT2442 |
GeneIDi | 23491418 |
KEGGi | mtc:MT2442 |
PATRICi | fig|83331.31.peg.2632 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000516 Genomic DNA Translation: AAK46736.1 |
PIRi | D70587 |
RefSeqi | WP_003899294.1, NZ_KK341227.1 |
3D structure databases
SMRi | P9WNV6 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | AAK46736; AAK46736; MT2442 |
GeneIDi | 23491418 |
KEGGi | mtc:MT2442 |
PATRICi | fig|83331.31.peg.2632 |
Phylogenomic databases
HOGENOMi | CLU_017633_0_7_11 |
Family and domain databases
CDDi | cd06257, DnaJ, 1 hit cd10719, DnaJ_zf, 1 hit |
Gene3Di | 1.10.287.110, 1 hit |
HAMAPi | MF_01152, DnaJ, 1 hit |
InterProi | View protein in InterPro IPR012724, DnaJ IPR002939, DnaJ_C IPR001623, DnaJ_domain IPR008971, HSP40/DnaJ_pept-bd IPR001305, HSP_DnaJ_Cys-rich_dom IPR036410, HSP_DnaJ_Cys-rich_dom_sf IPR036869, J_dom_sf |
Pfami | View protein in Pfam PF00226, DnaJ, 1 hit PF01556, DnaJ_C, 1 hit PF00684, DnaJ_CXXCXGXG, 1 hit |
PRINTSi | PR00625, JDOMAIN |
SMARTi | View protein in SMART SM00271, DnaJ, 1 hit |
SUPFAMi | SSF46565, SSF46565, 1 hit SSF49493, SSF49493, 2 hits SSF57938, SSF57938, 1 hit |
PROSITEi | View protein in PROSITE PS50076, DNAJ_2, 1 hit PS51188, ZF_CR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DNAJ2_MYCTO | |
Accessioni | P9WNV6Primary (citable) accession number: P9WNV6 Secondary accession number(s): L0TCB9, O05825, P63966 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 35 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |