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UniProtKB - P9WNS5 (DUT_MYCTU)
Protein
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Gene
dut
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.
UniRule annotationMiscellaneous
Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions.1 Publication
Catalytic activityi
- EC:3.6.1.23UniRule annotation1 Publication
Cofactori
Mg2+UniRule annotation1 Publication
: dUMP biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 77 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 91 | Substrate; via amide nitrogen and carbonyl oxygen1 Publication | 1 |
GO - Molecular functioni
- dUTP diphosphatase activity Source: MTBBASE
- magnesium ion binding Source: MTBBASE
GO - Biological processi
- dUMP biosynthetic process Source: GO_Central
- dUTP catabolic process Source: GO_Central
- dUTP metabolic process Source: MTBBASE
Keywordsi
Molecular function | Hydrolase |
Biological process | Nucleotide metabolism |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.6.1.23, 3445 |
UniPathwayi | UPA00610;UER00666 |
Names & Taxonomyi
Protein namesi | Recommended name: Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation1 Publication)Short name: dUTPase1 PublicationUniRule annotation Alternative name(s): dUTP pyrophosphataseUniRule annotation |
Gene namesi | Name:dut Ordered Locus Names:Rv2697c ORF Names:MTCY05A6.18c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2697c |
Pathology & Biotechi
Chemistry databases
DrugBanki | DB01965, 2'-Deoxyuridine 5'-alpha,beta-imido-triphosphate DB03800, Deoxyuridine monophosphate DB03413, Deoxyuridine-5'-Diphosphate DB02333, Deoxyuridine-5'-Triphosphate |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000182884 | 1 – 154 | Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST | 154 |
Proteomic databases
PaxDbi | P9WNS5 |
Interactioni
Subunit structurei
Homotrimer.
UniRule annotation1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv2697c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WNS5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 64 – 66 | Substrate bindingUniRule annotation1 Publication | 3 | |
Regioni | 81 – 83 | Substrate bindingUniRule annotation1 Publication | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0756, Bacteria |
OMAi | YAAFVHP |
PhylomeDBi | P9WNS5 |
Family and domain databases
CDDi | cd07557, trimeric_dUTPase, 1 hit |
Gene3Di | 2.70.40.10, 1 hit |
HAMAPi | MF_00116, dUTPase_bact, 1 hit |
InterProi | View protein in InterPro IPR029054, dUTPase-like IPR036157, dUTPase-like_sf IPR008181, dUTPase_1 IPR033704, dUTPase_trimeric |
PANTHERi | PTHR11241, PTHR11241, 1 hit |
Pfami | View protein in Pfam PF00692, dUTPase, 1 hit |
SUPFAMi | SSF51283, SSF51283, 1 hit |
TIGRFAMsi | TIGR00576, dut, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WNS5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTTLAIVRL DPGLPLPSRA HDGDAGVDLY SAEDVELAPG RRALVRTGVA
60 70 80 90 100
VAVPFGMVGL VHPRSGLATR VGLSIVNSPG TIDAGYRGEI KVALINLDPA
110 120 130 140 150
APIVVHRGDR IAQLLVQRVE LVELVEVSSF DEAGLASTSR GDGGHGSSGG
HASL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45495.1 |
PIRi | D70530 |
RefSeqi | NP_217213.1, NC_000962.3 WP_003413930.1, NZ_NVQJ01000017.1 |
Genome annotation databases
GeneIDi | 45426685 887290 |
KEGGi | mtu:Rv2697c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45495.1 |
PIRi | D70530 |
RefSeqi | NP_217213.1, NC_000962.3 WP_003413930.1, NZ_NVQJ01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MQ7 | X-ray | 1.95 | A | 1-154 | [»] | |
1SIX | X-ray | 1.30 | A | 1-154 | [»] | |
1SJN | X-ray | 1.80 | A/B/C | 1-154 | [»] | |
1SLH | X-ray | 3.00 | A/B/C | 1-154 | [»] | |
1SM8 | X-ray | 2.90 | A/B/C | 1-154 | [»] | |
1SMC | X-ray | 2.10 | A/B/C | 1-154 | [»] | |
1SNF | X-ray | 1.85 | A/B/C | 1-154 | [»] | |
2PY4 | X-ray | 1.49 | A | 1-154 | [»] | |
3H6D | X-ray | 1.80 | A | 1-154 | [»] | |
3HZA | X-ray | 1.20 | A | 1-154 | [»] | |
3I93 | X-ray | 1.80 | A | 1-138 | [»] | |
3LOJ | X-ray | 1.25 | A | 1-154 | [»] | |
4GCY | X-ray | 1.50 | A | 1-154 | [»] | |
5ECT | X-ray | 1.30 | A | 1-142 | [»] | |
5EDD | X-ray | 1.97 | A | 1-154 | [»] | |
SMRi | P9WNS5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2697c |
Chemistry databases
DrugBanki | DB01965, 2'-Deoxyuridine 5'-alpha,beta-imido-triphosphate DB03800, Deoxyuridine monophosphate DB03413, Deoxyuridine-5'-Diphosphate DB02333, Deoxyuridine-5'-Triphosphate |
Proteomic databases
PaxDbi | P9WNS5 |
Genome annotation databases
GeneIDi | 45426685 887290 |
KEGGi | mtu:Rv2697c |
Organism-specific databases
TubercuListi | Rv2697c |
Phylogenomic databases
eggNOGi | COG0756, Bacteria |
OMAi | YAAFVHP |
PhylomeDBi | P9WNS5 |
Enzyme and pathway databases
UniPathwayi | UPA00610;UER00666 |
BRENDAi | 3.6.1.23, 3445 |
Family and domain databases
CDDi | cd07557, trimeric_dUTPase, 1 hit |
Gene3Di | 2.70.40.10, 1 hit |
HAMAPi | MF_00116, dUTPase_bact, 1 hit |
InterProi | View protein in InterPro IPR029054, dUTPase-like IPR036157, dUTPase-like_sf IPR008181, dUTPase_1 IPR033704, dUTPase_trimeric |
PANTHERi | PTHR11241, PTHR11241, 1 hit |
Pfami | View protein in Pfam PF00692, dUTPase, 1 hit |
SUPFAMi | SSF51283, SSF51283, 1 hit |
TIGRFAMsi | TIGR00576, dut, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DUT_MYCTU | |
Accessioni | P9WNS5Primary (citable) accession number: P9WNS5 Secondary accession number(s): L0TAD4, O07199, P0A552 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 23, 2022 | |
This is version 45 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families