UniProtKB - P9WNS1 (DXR_MYCTU)
Protein
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Gene
dxr
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation
Catalytic activityi
- 2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + H+ + NADPHUniRule annotationEC:1.1.1.267UniRule annotation
Cofactori
a divalent metal cationUniRule annotation
: isopentenyl diphosphate biosynthesis via DXP pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotationProteins known to be involved in the 6 steps of the subpathway in this organism are:
- 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
- 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
- 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
- 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
- 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
- 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 (ispH2), 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 (ispH1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 128 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 151 | Divalent metal cationUniRule annotation | 1 | |
Metal bindingi | 153 | Divalent metal cationUniRule annotation | 1 | |
Binding sitei | 153 | SubstrateUniRule annotation | 1 | |
Binding sitei | 177 | SubstrateUniRule annotation | 1 | |
Binding sitei | 200 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 222 | Divalent metal cationUniRule annotation | 1 | |
Binding sitei | 222 | SubstrateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 18 – 47 | NADPUniRule annotationAdd BLAST | 30 |
GO - Molecular functioni
- 1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Source: MTBBASE
- cobalt ion binding Source: MTBBASE
- magnesium ion binding Source: MTBBASE
- manganese ion binding Source: MTBBASE
- NADPH binding Source: MTBBASE
GO - Biological processi
- isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway involved in terpenoid biosynthetic process Source: MTBBASE
- terpenoid biosynthetic process, mevalonate-independent Source: MTBBASE
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Isoprene biosynthesis |
Ligand | Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7122-MONOMER |
UniPathwayi | UPA00056;UER00092 |
Chemistry databases
SwissLipidsi | SLP:000001174 |
Names & Taxonomyi
Protein namesi | Recommended name: 1-deoxy-D-xylulose 5-phosphate reductoisomeraseUniRule annotation (EC:1.1.1.267UniRule annotation)Short name: DXP reductoisomeraseUniRule annotation Alternative name(s): 1-deoxyxylulose-5-phosphate reductoisomeraseUniRule annotation 2-C-methyl-D-erythritol 4-phosphate synthaseUniRule annotation |
Gene namesi | Name:dxrUniRule annotation Ordered Locus Names:Rv2870c ORF Names:MTCY274.01c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2870c |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000163678 | 1 – 413 | 1-deoxy-D-xylulose 5-phosphate reductoisomeraseAdd BLAST | 413 |
Proteomic databases
PaxDbi | P9WNS1 |
PRIDEi | P9WNS1 |
Interactioni
Protein-protein interaction databases
STRINGi | 83332.Rv2870c |
Chemistry databases
BindingDBi | P9WNS1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WNS1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the DXR family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105CEA Bacteria COG0743 LUCA |
KOi | K00099 |
OMAi | AANEECV |
PhylomeDBi | P9WNS1 |
Family and domain databases
HAMAPi | MF_00183 DXP_reductoisom, 1 hit |
InterProi | View protein in InterPro IPR003821 DXP_reductoisomerase IPR013644 DXP_reductoisomerase_C IPR013512 DXP_reductoisomerase_N IPR026877 DXPR_C IPR036169 DXPR_C_sf IPR036291 NAD(P)-bd_dom_sf |
PANTHERi | PTHR30525 PTHR30525, 1 hit |
Pfami | View protein in Pfam PF08436 DXP_redisom_C, 1 hit PF02670 DXP_reductoisom, 1 hit PF13288 DXPR_C, 1 hit |
PIRSFi | PIRSF006205 Dxp_reductismrs, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit SSF69055 SSF69055, 1 hit |
TIGRFAMsi | TIGR00243 Dxr, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WNS1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTNSTDGRAD GRLRVVVLGS TGSIGTQALQ VIADNPDRFE VVGLAAGGAH
60 70 80 90 100
LDTLLRQRAQ TGVTNIAVAD EHAAQRVGDI PYHGSDAATR LVEQTEADVV
110 120 130 140 150
LNALVGALGL RPTLAALKTG ARLALANKES LVAGGSLVLR AARPGQIVPV
160 170 180 190 200
DSEHSALAQC LRGGTPDEVA KLVLTASGGP FRGWSAADLE HVTPEQAGAH
210 220 230 240 250
PTWSMGPMNT LNSASLVNKG LEVIETHLLF GIPYDRIDVV VHPQSIIHSM
260 270 280 290 300
VTFIDGSTIA QASPPDMKLP ISLALGWPRR VSGAAAACDF HTASSWEFEP
310 320 330 340 350
LDTDVFPAVE LARQAGVAGG CMTAVYNAAN EEAAAAFLAG RIGFPAIVGI
360 370 380 390 400
IADVLHAADQ WAVEPATVDD VLDAQRWARE RAQRAVSGMA SVAIASTAKP
410
GAAGRHASTL ERS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45672.1 |
PIRi | A70923 |
RefSeqi | NP_217386.2, NC_000962.3 WP_003414613.1, NZ_NVQJ01000006.1 |
Genome annotation databases
EnsemblBacteriai | CCP45672; CCP45672; Rv2870c |
GeneIDi | 887800 |
KEGGi | mtu:Rv2870c mtv:RVBD_2870c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45672.1 |
PIRi | A70923 |
RefSeqi | NP_217386.2, NC_000962.3 WP_003414613.1, NZ_NVQJ01000006.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2C82 | X-ray | 1.90 | A/B | 1-413 | [»] | |
2JCV | X-ray | 2.20 | A/B | 2-389 | [»] | |
2JCX | X-ray | 2.10 | A/B | 2-389 | [»] | |
2JCY | X-ray | 2.35 | A/B | 2-389 | [»] | |
2JD0 | X-ray | 2.30 | A/B | 2-389 | [»] | |
2JD1 | X-ray | 2.00 | A/B | 2-389 | [»] | |
2JD2 | X-ray | 2.15 | A/B | 2-389 | [»] | |
2Y1C | X-ray | 1.90 | A/B | 2-389 | [»] | |
2Y1D | X-ray | 2.05 | A/B | 2-389 | [»] | |
2Y1E | X-ray | 1.65 | A/B | 2-389 | [»] | |
2Y1F | X-ray | 1.96 | A/B | 2-389 | [»] | |
2Y1G | X-ray | 1.95 | A/B | 2-389 | [»] | |
3RAS | X-ray | 2.55 | A/B | 2-389 | [»] | |
3ZHX | X-ray | 2.00 | A/B | 2-389 | [»] | |
3ZHY | X-ray | 2.30 | A/B | 2-389 | [»] | |
3ZHZ | X-ray | 2.25 | A/B | 2-389 | [»] | |
3ZI0 | X-ray | 1.90 | A/B | 2-389 | [»] | |
4A03 | X-ray | 1.65 | A/B | 2-389 | [»] | |
4AIC | X-ray | 2.05 | A/B | 2-389 | [»] | |
4OOE | X-ray | 1.83 | A/B/C/D | 1-389 | [»] | |
4OOF | X-ray | 2.30 | A/B | 1-389 | [»] | |
4RCV | X-ray | 2.29 | A/B | 1-389 | [»] | |
SMRi | P9WNS1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2870c |
Chemistry databases
BindingDBi | P9WNS1 |
ChEMBLi | CHEMBL5630 |
SwissLipidsi | SLP:000001174 |
Proteomic databases
PaxDbi | P9WNS1 |
PRIDEi | P9WNS1 |
Genome annotation databases
EnsemblBacteriai | CCP45672; CCP45672; Rv2870c |
GeneIDi | 887800 |
KEGGi | mtu:Rv2870c mtv:RVBD_2870c |
Organism-specific databases
TubercuListi | Rv2870c |
Phylogenomic databases
eggNOGi | ENOG4105CEA Bacteria COG0743 LUCA |
KOi | K00099 |
OMAi | AANEECV |
PhylomeDBi | P9WNS1 |
Enzyme and pathway databases
UniPathwayi | UPA00056;UER00092 |
BioCyci | MTBH37RV:G185E-7122-MONOMER |
Family and domain databases
HAMAPi | MF_00183 DXP_reductoisom, 1 hit |
InterProi | View protein in InterPro IPR003821 DXP_reductoisomerase IPR013644 DXP_reductoisomerase_C IPR013512 DXP_reductoisomerase_N IPR026877 DXPR_C IPR036169 DXPR_C_sf IPR036291 NAD(P)-bd_dom_sf |
PANTHERi | PTHR30525 PTHR30525, 1 hit |
Pfami | View protein in Pfam PF08436 DXP_redisom_C, 1 hit PF02670 DXP_reductoisom, 1 hit PF13288 DXPR_C, 1 hit |
PIRSFi | PIRSF006205 Dxp_reductismrs, 1 hit |
SUPFAMi | SSF51735 SSF51735, 1 hit SSF69055 SSF69055, 1 hit |
TIGRFAMsi | TIGR00243 Dxr, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DXR_MYCTU | |
Accessioni | P9WNS1Primary (citable) accession number: P9WNS1 Secondary accession number(s): L0TDT3, P64012, Q10798 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 37 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references