UniProtKB - P9WNM5 (CYSNC_MYCTU)
Protein
Bifunctional enzyme CysN/CysC
Gene
cysNC
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.By similarity
APS kinase catalyzes the synthesis of activated sulfate.By similarity
Catalytic activityi
: hydrogen sulfide biosynthesis Pathwayi
This protein is involved in step 1 and 2 of the subpathway that synthesizes sulfite from sulfate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Sulfate adenylyltransferase subunit 2 (cysD), Bifunctional enzyme CysN/CysC (cysNC)
- Bifunctional enzyme CysN/CysC (cysNC)
- Probable phosphoadenosine phosphosulfate reductase (cysH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 524 | Phosphoserine intermediateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 18 | GTPBy similarity | 8 | |
Nucleotide bindingi | 88 – 92 | GTPBy similarity | 5 | |
Nucleotide bindingi | 143 – 146 | GTPBy similarity | 4 | |
Nucleotide bindingi | 450 – 457 | ATPSequence analysis | 8 |
GO - Molecular functioni
- adenylylsulfate kinase activity Source: MTBBASE
- ATP binding Source: UniProtKB-UniRule
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-UniRule
- sulfate adenylyltransferase (ATP) activity Source: UniProtKB-UniRule
GO - Biological processi
- cellular response to oxidative stress Source: MTBBASE
- cellular response to sulfur starvation Source: MTBBASE
- hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
- sulfate assimilation Source: Reactome
- sulfate assimilation via adenylyl sulfate reduction Source: MTBBASE
Keywordsi
Molecular function | Kinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase |
Ligand | ATP-binding, GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MTU-936635, Sulfate assimilation |
UniPathwayi | UPA00140;UER00204 UPA00140;UER00205 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional enzyme CysN/CysCIncluding the following 2 domains: |
Gene namesi | Name:cysNC Synonyms:cysN Ordered Locus Names:Rv1286 ORF Names:MTCY373.05 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1286 |
Subcellular locationi
GO - Cellular componenti
- cytosol Source: Reactome
- plasma membrane Source: MTBBASE
- sulfate adenylyltransferase complex (ATP) Source: MTBBASE
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000091537 | 1 – 614 | Bifunctional enzyme CysN/CysCAdd BLAST | 614 |
Proteomic databases
PaxDbi | P9WNM5 |
Expressioni
Inductioni
Induced by sulfur limitation and oxidative stress. Repressed by the presence of cysteine.1 Publication
Interactioni
Subunit structurei
Heterodimer composed of CysD, the smaller subunit, and CysNC.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv1286 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WNM5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 217 | tr-type GAdd BLAST | 216 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 441 | Sulfate adenylyltransferaseAdd BLAST | 441 | |
Regioni | 11 – 18 | G1By similarity | 8 | |
Regioni | 67 – 71 | G2By similarity | 5 | |
Regioni | 88 – 91 | G3By similarity | 4 | |
Regioni | 143 – 146 | G4By similarity | 4 | |
Regioni | 180 – 182 | G5By similarity | 3 | |
Regioni | 442 – 614 | Adenylyl-sulfate kinaseAdd BLAST | 173 |
Sequence similaritiesi
In the C-terminal section; belongs to the APS kinase family.Curated
In the N-terminal section; belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.Curated
Phylogenomic databases
eggNOGi | COG0529, Bacteria COG2895, Bacteria |
OMAi | SRGDMIC |
PhylomeDBi | P9WNM5 |
Family and domain databases
CDDi | cd02027, APSK, 1 hit cd04166, CysN_ATPS, 1 hit |
HAMAPi | MF_00065, Adenylyl_sulf_kinase, 1 hit MF_00062, Sulf_adenylyltr_sub1, 1 hit |
InterProi | View protein in InterPro IPR002891, APS_kinase IPR041757, CysN_GTP-bd IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR011779, SO4_adenylTrfase_lsu IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00455, apsK, 1 hit TIGR02034, CysN, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WNM5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTLLRLATA GSVDDGKSTL IGRLLYDSKA VMEDQWASVE QTSKDRGHDY
60 70 80 90 100
TDLALVTDGL RAEREQGITI DVAYRYFATP KRKFIIADTP GHIQYTRNMV
110 120 130 140 150
TGASTAQLVI VLVDARHGLL EQSRRHAFLA SLLGIRHLVL AVNKMDLLGW
160 170 180 190 200
DQEKFDAIRD EFHAFAARLD VQDVTSIPIS ALHGDNVVTK SDQTPWYEGP
210 220 230 240 250
SLLSHLEDVY IAGDRNMVDV RFPVQYVIRP HTLEHQDHRS YAGTVASGVM
260 270 280 290 300
RSGDEVVVLP IGKTTRITAI DGPNGPVAEA FPPMAVSVRL ADDIDISRGD
310 320 330 340 350
MIARTHNQPR ITQEFDATVC WMADNAVLEP GRDYVVKHTT RTVRARIAGL
360 370 380 390 400
DYRLDVNTLH RDKTATALKL NELGRVSLRT QVPLLLDEYT RNASTGSFIL
410 420 430 440 450
IDPDTNGTVA AGMVLRDVSA RTPSPNTVRH RSLVTAQDRP PRGKTVWFTG
460 470 480 490 500
LSGSGKSSVA MLVERKLLEK GISAYVLDGD NLRHGLNADL GFSMADRAEN
510 520 530 540 550
LRRLSHVATL LADCGHLVLV PAISPLAEHR ALARKVHADA GIDFFEVFCD
560 570 580 590 600
TPLQDCERRD PKGLYAKARA GEITHFTGID SPYQRPKNPD LRLTPDRSID
610
EQAQEVIDLL ESSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44042.1 |
PIRi | B70772 |
RefSeqi | NP_215802.1, NC_000962.3 WP_003406621.1, NZ_NVQJ01000030.1 |
Genome annotation databases
EnsemblBacteriai | CCP44042; CCP44042; Rv1286 |
GeneIDi | 23492568 886978 |
KEGGi | mtu:Rv1286 mtv:RVBD_1286 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44042.1 |
PIRi | B70772 |
RefSeqi | NP_215802.1, NC_000962.3 WP_003406621.1, NZ_NVQJ01000030.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4BZQ | X-ray | 2.10 | A/B | 440-612 | [»] | |
4BZX | X-ray | 1.70 | A/B | 440-612 | [»] | |
4RFV | X-ray | 1.69 | A/B | 424-612 | [»] | |
SMRi | P9WNM5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1286 |
Proteomic databases
PaxDbi | P9WNM5 |
Genome annotation databases
EnsemblBacteriai | CCP44042; CCP44042; Rv1286 |
GeneIDi | 23492568 886978 |
KEGGi | mtu:Rv1286 mtv:RVBD_1286 |
Organism-specific databases
TubercuListi | Rv1286 |
Phylogenomic databases
eggNOGi | COG0529, Bacteria COG2895, Bacteria |
OMAi | SRGDMIC |
PhylomeDBi | P9WNM5 |
Enzyme and pathway databases
UniPathwayi | UPA00140;UER00204 UPA00140;UER00205 |
Reactomei | R-MTU-936635, Sulfate assimilation |
Family and domain databases
CDDi | cd02027, APSK, 1 hit cd04166, CysN_ATPS, 1 hit |
HAMAPi | MF_00065, Adenylyl_sulf_kinase, 1 hit MF_00062, Sulf_adenylyltr_sub1, 1 hit |
InterProi | View protein in InterPro IPR002891, APS_kinase IPR041757, CysN_GTP-bd IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR011779, SO4_adenylTrfase_lsu IPR000795, TF_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 2 hits |
TIGRFAMsi | TIGR00455, apsK, 1 hit TIGR02034, CysN, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYSNC_MYCTU | |
Accessioni | P9WNM5Primary (citable) accession number: P9WNM5 Secondary accession number(s): L0T6E9, Q10600 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 42 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families