UniProtKB - P9WNG5 (FABD_MYCTU)
Protein
Malonyl CoA-acyl carrier protein transacylase
Gene
fabD
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Miscellaneous
Was identified as a natural substrate of the M.tuberculosis proteasome.
Was identified as a high-confidence drug target.
Catalytic activityi
- EC:2.3.1.39
: fatty acid biosynthesis Pathwayi
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 91 | By similarity | 1 | |
Active sitei | 194 | By similarity | 1 |
GO - Molecular functioni
- [acyl-carrier-protein] S-malonyltransferase activity Source: MTBBASE
GO - Biological processi
- fatty acid biosynthetic process Source: MTBBASE
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-6458-MONOMER |
UniPathwayi | UPA00094 |
Chemistry databases
SwissLipidsi | SLP:000000965 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fabD Ordered Locus Names:Rv2243 ORF Names:MTCY427.24 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2243 |
Subcellular locationi
GO - Cellular componenti
- cytosol Source: GO_Central
- fatty acid synthase complex Source: MTBBASE
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 173 | K → A: Nearly abolishes pupylation and dramatically stabilizes this proteasome substrate in wild-type mycobacteria. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194218 | 1 – 302 | Malonyl CoA-acyl carrier protein transacylaseAdd BLAST | 302 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 173 | Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)2 Publications |
Post-translational modificationi
Pupylated at Lys-173 by the prokaryotic ubiquitin-like protein Pup, which leads to its degradation by the proteasome.2 Publications
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | P9WNG5 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WNG5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 206 – 210 | Poly-Ala | 5 | |
Compositional biasi | 233 – 236 | Poly-Ala | 4 |
Sequence similaritiesi
Belongs to the FabD family.Curated
Phylogenomic databases
eggNOGi | ENOG4105CJF Bacteria COG0331 LUCA |
KOi | K00645 |
OMAi | AANYNCP |
PhylomeDBi | P9WNG5 |
Family and domain databases
Gene3Di | 3.40.366.10, 1 hit |
InterProi | View protein in InterPro IPR001227 Ac_transferase_dom_sf IPR014043 Acyl_transferase IPR016035 Acyl_Trfase/lysoPLipase IPR016036 Malonyl_transacylase_ACP-bd IPR020801 PKS_acyl_transferase |
Pfami | View protein in Pfam PF00698 Acyl_transf_1, 1 hit |
SMARTi | View protein in SMART SM00827 PKS_AT, 1 hit |
SUPFAMi | SSF52151 SSF52151, 1 hit SSF55048 SSF55048, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WNG5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIALLAPGQG SQTEGMLSPW LQLPGAADQI AAWSKAADLD LARLGTTAST
60 70 80 90 100
EEITDTAVAQ PLIVAATLLA HQELARRCVL AGKDVIVAGH SVGEIAAYAI
110 120 130 140 150
AGVIAADDAV ALAATRGAEM AKACATEPTG MSAVLGGDET EVLSRLEQLD
160 170 180 190 200
LVPANRNAAG QIVAAGRLTA LEKLAEDPPA KARVRALGVA GAFHTEFMAP
210 220 230 240 250
ALDGFAAAAA NIATADPTAT LLSNRDGKPV TSAAAAMDTL VSQLTQPVRW
260 270 280 290 300
DLCTATLREH TVTAIVEFPP AGTLSGIAKR ELRGVPARAV KSPADLDELA
NL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45023.1 |
PIRi | G70778 |
RefSeqi | NP_216759.1, NC_000962.3 WP_003411559.1, NZ_NVQJ01000008.1 |
Genome annotation databases
EnsemblBacteriai | CCP45023; CCP45023; Rv2243 |
GeneIDi | 888769 |
KEGGi | mtu:Rv2243 mtv:RVBD_2243 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45023.1 |
PIRi | G70778 |
RefSeqi | NP_216759.1, NC_000962.3 WP_003411559.1, NZ_NVQJ01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2QC3 | X-ray | 2.30 | A | 1-302 | [»] | |
2QJ3 | X-ray | 3.00 | A/B | 1-302 | [»] | |
SMRi | P9WNG5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2243 |
Chemistry databases
SwissLipidsi | SLP:000000965 |
Proteomic databases
PaxDbi | P9WNG5 |
Genome annotation databases
EnsemblBacteriai | CCP45023; CCP45023; Rv2243 |
GeneIDi | 888769 |
KEGGi | mtu:Rv2243 mtv:RVBD_2243 |
Organism-specific databases
TubercuListi | Rv2243 |
Phylogenomic databases
eggNOGi | ENOG4105CJF Bacteria COG0331 LUCA |
KOi | K00645 |
OMAi | AANYNCP |
PhylomeDBi | P9WNG5 |
Enzyme and pathway databases
UniPathwayi | UPA00094 |
BioCyci | MTBH37RV:G185E-6458-MONOMER |
Family and domain databases
Gene3Di | 3.40.366.10, 1 hit |
InterProi | View protein in InterPro IPR001227 Ac_transferase_dom_sf IPR014043 Acyl_transferase IPR016035 Acyl_Trfase/lysoPLipase IPR016036 Malonyl_transacylase_ACP-bd IPR020801 PKS_acyl_transferase |
Pfami | View protein in Pfam PF00698 Acyl_transf_1, 1 hit |
SMARTi | View protein in SMART SM00827 PKS_AT, 1 hit |
SUPFAMi | SSF52151 SSF52151, 1 hit SSF55048 SSF55048, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FABD_MYCTU | |
Accessioni | P9WNG5Primary (citable) accession number: P9WNG5 Secondary accession number(s): L0TAL7, P63458, Q10501 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references