UniProtKB - P9WMZ3 (PIMB_MYCTU)
Protein
GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase
Gene
pimB
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM) (PubMed:19395496). Catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (PubMed:19395496). PimB also catalyzes the addition of a mannosyl residue from GDP-Man to the position 6 of phosphatidyl-myo-inositol bearing an acylated alpha-1,2-linked mannose residue (Ac1PIM1) to generate monoacylated phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose residues (Ac1PIM2) (By similarity). The addition of the second mannosyl residue by PimB preferentially occurs before the acylation of the mannosyl residue transferred by PimA (By similarity). Also able to transfer a mannosyl residue from GDP-Man to the position 6 of a phosphatidyl-myo-inositol (PI), but this reaction is very slow (By similarity).By similarity1 Publication
Catalytic activityi
- 1,2-diacyl-sn-glycero-3-phospho-[α-D-mannopyranosyl-(1→2)-D-myo-inositol] + GDP-α-D-mannose = a 2,6-O-bis(α-D-mannopyranosyl)-1-phosphatidyl-1D-myo-inositol + GDP + H+By similarityEC:2.4.1.346By similarity
- 1,2-diacyl-sn-glycero-3-phospho-[α-D-6-acyl-mannopyranosyl-(1→2)-D-myo-inositol] + GDP-α-D-mannose = 2-O-(α-D-mannosyl)-6-O-(6-O-acyl-α-D-mannosyl)-1-phosphatidyl-1D-myo-inositol + GDP + H+By similarityEC:2.4.1.346By similarity
: phosphatidylinositol metabolism Pathwayi
This protein is involved in the pathway phosphatidylinositol metabolism, which is part of Phospholipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway phosphatidylinositol metabolism and in Phospholipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 205 | GDP-mannoseBy similarity | 1 | |
Binding sitei | 210 | GDP-mannoseBy similarity | 1 | |
Binding sitei | 262 | GDP-mannose; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 299 | GDP-mannoseBy similarity | 1 |
GO - Molecular functioni
- glycolipid 6-alpha-mannosyltransferase activity Source: UniProtKB
- mannosyltransferase activity Source: MTBBASE
- phosphatidylinositol alpha-mannosyltransferase activity Source: UniProtKB
GO - Biological processi
- glycolipid biosynthetic process Source: UniProtKB
- pathogenesis Source: UniProtKB-KW
- phosphatidylinositol metabolic process Source: UniProtKB-UniPathway
- phospholipid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Virulence |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-6398-MONOMER |
UniPathwayi | UPA00949 |
Names & Taxonomyi
Protein namesi | Recommended name: GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferase1 Publication (EC:2.4.1.346By similarity)Alternative name(s): Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferaseBy similarity Alpha-mannosyltransferaseBy similarity Short name: Alpha-ManTBy similarity Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferaseCurated Phosphatidylinositol alpha-mannosyltransferaseBy similarity Short name: PI alpha-mannosyltransferaseBy similarity |
Gene namesi | Name:pimB1 Publication Ordered Locus Names:Rv2188c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2188c |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000393733 | 1 – 385 | GDP-mannose-dependent alpha-(1-6)-phosphatidylinositol monomannoside mannosyltransferaseAdd BLAST | 385 |
Proteomic databases
PaxDbi | P9WMZ3 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105JNK Bacteria COG0438 LUCA |
KOi | K13668 |
OMAi | FDAEQPF |
PhylomeDBi | P9WMZ3 |
Family and domain databases
InterProi | View protein in InterPro IPR001296 Glyco_trans_1 IPR028098 Glyco_trans_4-like_N |
Pfami | View protein in Pfam PF13439 Glyco_transf_4, 1 hit PF00534 Glycos_transf_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WMZ3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRVLLVTND FPPRRGGIQS YLGEFVGRLV GSRAHAMTVY APQWKGADAF
60 70 80 90 100
DDAARAAGYR VVRHPSTVML PGPTVDVRMR RLIAEHDIET VWFGAAAPLA
110 120 130 140 150
LLAPRARLAG ASRVLASTHG HEVGWSMLPV ARSVLRRIGD GTDVVTFVSS
160 170 180 190 200
YTRSRFASAF GPAASLEYLP PGVDTDRFRP DPAARAELRK RYRLGERPTV
210 220 230 240 250
VCLSRLVPRK GQDTLVTALP SIRRRVDGAA LVIVGGGPYL ETLRKLAHDC
260 270 280 290 300
GVADHVTFTG GVATDELPAH HALADVFAMP CRTRGAGMDV EGLGIVFLEA
310 320 330 340 350
SAAGVPVIAG NSGGAPETVQ HNKTGLVVDG RSVDRVADAV AELLIDRDRA
360 370 380
VAMGAAGREW VTAQWRWDTL AAKLADFLRG DDAAR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44965.1 |
PIRi | F70937 |
RefSeqi | NP_216704.2, NC_000962.3 WP_003411369.1, NZ_NVQJ01000008.1 |
Genome annotation databases
EnsemblBacteriai | CCP44965; CCP44965; Rv2188c |
GeneIDi | 887278 |
KEGGi | mtu:Rv2188c mtv:RVBD_2188c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44965.1 |
PIRi | F70937 |
RefSeqi | NP_216704.2, NC_000962.3 WP_003411369.1, NZ_NVQJ01000008.1 |
3D structure databases
SMRi | P9WMZ3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2188c |
Proteomic databases
PaxDbi | P9WMZ3 |
Genome annotation databases
EnsemblBacteriai | CCP44965; CCP44965; Rv2188c |
GeneIDi | 887278 |
KEGGi | mtu:Rv2188c mtv:RVBD_2188c |
Organism-specific databases
TubercuListi | Rv2188c |
Phylogenomic databases
eggNOGi | ENOG4105JNK Bacteria COG0438 LUCA |
KOi | K13668 |
OMAi | FDAEQPF |
PhylomeDBi | P9WMZ3 |
Enzyme and pathway databases
UniPathwayi | UPA00949 |
BioCyci | MTBH37RV:G185E-6398-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR001296 Glyco_trans_1 IPR028098 Glyco_trans_4-like_N |
Pfami | View protein in Pfam PF13439 Glyco_transf_4, 1 hit PF00534 Glycos_transf_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PIMB_MYCTU | |
Accessioni | P9WMZ3Primary (citable) accession number: P9WMZ3 Secondary accession number(s): L0T936, O53522, Q7D7D7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways