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UniProtKB - P9WMZ1 (GLGM_MYCTU)

Entry version 33 (16 Oct 2019)
Sequence version 1 (16 Apr 2014)
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Protein

Alpha-maltose-1-phosphate synthase

Gene

glgM

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the biosynthesis of the maltose-1-phosphate (M1P) building block required for alpha-glucan production by the key enzyme GlgE (PubMed:18808383, PubMed:27513637). Catalyzes the formation of an alpha-1,4 linkage between glucose from ADP-glucose and glucose 1-phosphate (G1P) to yield maltose-1-phosphate (M1P) (PubMed:27513637). Also able to catalyze the elongation of the non-reducing ends of glycogen, maltodextrin and maltoheptaose using ADP-glucose as sugar donor, however the rate of the reaction appears to be too low to be physiologically relevant (PubMed:27513637). GlgM is also able to use UDP-glucose as sugar donor with G1P, however, it is less efficient than with ADP-glucose (PubMed:27513637). UDP-glucose is not used as sugar donor when glycogen is used as acceptor (PubMed:27513637).2 Publications

Miscellaneous

Maltose-1-phosphate (M1P) is generated by two alternative routes: the TreS-Pep2 branch and the GlgC-GlgM branch, however it seems that TreS-Pep2 branch provides most of M1P for the GlgE pathway in M.tuberculosis.1 Publication
Attempts to disrupt both the Rv3032 gene and glgA in order to create a mutant simultaneously deficient in both alpha-1,4-glucosyltransferases turned out to be unsuccessful. Thus, M.tuberculosis H37Rv requires a functional copy of at least one of these two genes for growth.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited at high G1P concentrations.1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

Kcat is 97.9 sec(-1) for G1P as substrate (with 0.5 mM of ADP-glucose). Kcat is 80.6 sec(-1) for G1P as substrate (with 4 mM of ADP-glucose). Kcat is 39 sec(-1) for G1P as substrate (with 0.1 mM of ADP-glucose). Kcat is 29.2 sec(-1) for ADP-glucose as substrate (with 1 mM of G1P). Kcat is 20 sec(-1) for ADP-glucose as substrate (with 0.25 mM of G1P). Kcat is 19.8 sec(-1) for ADP-glucose as substrate (with 2.5 mM of G1P). Kcat is 15.4 sec(-1) for ADP-glucose as substrate (with 4 mM of G1P). Kcat is 0.09 sec(-1) for rabbit liver glycogen as substrate. Kcat is 0.014 sec(-1) for alpha-glucan as substrate.1 Publication
  1. KM=0.046 mM for ADP-glucose (with 4 mM of G1P)1 Publication
  2. KM=0.063 mM for ADP-glucose (with 2.5 mM of G1P)1 Publication
  3. KM=0.121 mM for ADP-glucose (with 1 mM of G1P)1 Publication
  4. KM=0.145 mM for ADP-glucose (with 0.25 mM of G1P)1 Publication
  5. KM=0.4 mM for alpha-glucan1 Publication
  6. KM=0.695 mM for G1P (with 4 mM of ADP-glucose)1 Publication
  7. KM=0.816 mM for G1P (with 0.1 mM of ADP-glucose)1 Publication
  8. KM=0.9 mM for rabbit liver glycogen1 Publication
  9. KM=1.290 mM for G1P (with 0.5 mM of ADP-glucose)1 Publication
  1. Vmax=136 µmol/min/mg enzyme toward G1P (with 0.5 mM of ADP-glucose)1 Publication
  2. Vmax=113 µmol/min/mg enzyme toward G1P (with 4 mM of ADP-glucose)1 Publication
  3. Vmax=54.2 µmol/min/mg enzyme toward G1P (with 0.1 mM of ADP-glucose)1 Publication
  4. Vmax=40.5 µmol/min/mg enzyme toward ADP-glucose (with 1 mM of G1P)1 Publication
  5. Vmax=27.7 µmol/min/mg enzyme toward ADP-glucose (with 0.25 mM of G1P)1 Publication
  6. Vmax=27.5 µmol/min/mg enzyme toward ADP-glucose (with 2.5 mM of G1P)1 Publication
  7. Vmax=21.4 µmol/min/mg enzyme toward ADP-glucose (with 4 mM of G1P)1 Publication
  8. Vmax=0.12 µmol/min/mg enzyme with rabbit liver glycogen as substrate1 Publication
  9. Vmax=0.02 µmol/min/mg enzyme with alpha-glucan as substrate1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: capsule polysaccharide biosynthesis

This protein is involved in the pathway capsule polysaccharide biosynthesis, which is part of Capsule biogenesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway capsule polysaccharide biosynthesis and in Capsule biogenesis.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCapsule biogenesis/degradation, Carbohydrate metabolism

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		MetaCyc:G185E-5382-MONOMER
MTBH37RV:G185E-5382-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00164
UPA00934

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Alpha-maltose-1-phosphate synthase1 Publication (EC:2.4.1.3421 Publication)
Short name:
M1P synthase1 Publication
Alternative name(s):
ADP-alpha-D-glucose:alpha-D-glucose-1-phosphate 4-alpha-D-glucosyltransferase1 Publication
M1P-producing glucosyltransferase1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:glgM1 Publication
Synonyms:glgA
Ordered Locus Names:Rv1212c
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83332 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

Organism-specific databases

Mycobacterium tuberculosis strain H37Rv genome database

More...TubercuListi		Rv1212c

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Inactivation of glgM affects the production of extracellular alpha-glucan (two-fold reduction), but not that of intracellular glycogen and 6-O-methylglucosyl lipopolysaccharides (MGLP) (PubMed:18808383, PubMed:27513637). Cells lacking this gene are also impaired in their ability to persist in both the spleen and the lungs of mice (PubMed:18808383). Combined inactivation of both glgM and ostA is lethal, potentially due to accumulation of toxic levels of ADP-glucose (PubMed:27513637). Combined inactivation of both glgM and treS results in absence of alpha-glucan (PubMed:27513637).2 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004139781 – 387Alpha-maltose-1-phosphate synthaseAdd BLAST387

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P9WMZ1

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...STRINGi		83332.Rv1212c

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P9WMZ1

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG4107QPH Bacteria
COG0438 LUCA

KEGG Orthology (KO)

More...KOi		K16148

Identification of Orthologs from Complete Genome Data

More...OMAi		WYTGLAG

Database for complete collections of gene phylogenies

More...PhylomeDBi		P9WMZ1

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR001296 Glyco_trans_1
IPR028098 Glyco_trans_4-like_N
IPR011875 M1P_synthase

Pfam protein domain database

More...Pfami		View protein in Pfam
PF13439 Glyco_transf_4, 1 hit
PF00534 Glycos_transf_1, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR02149 glgA_Coryne, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P9WMZ1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRVAMLTREY PPEVYGGAGV HVTELVAYLR RLCAVDVHCM GAPRPGAFAY 
        60         70         80         90        100
RPDPRLGSAN AALSTLSADL VMANAASAAT VVHSHTWYTA LAGHLAAILY 
       110        120        130        140        150
DIPHVLTAHS LEPLRPWKKE QLGGGYQVST WVEQTAVLAA NAVIAVSSAM 
       160        170        180        190        200
RNDMLRVYPS LDPNLVHVIR NGIDTETWYP AGPARTGSVL AELGVDPNRP 
       210        220        230        240        250
MAVFVGRITR QKGVVHLVTA AHRFRSDVQL VLCAGAADTP EVADEVRVAV 
       260        270        280        290        300
AELARNRTGV FWIQDRLTIG QLREILSAAT VFVCPSVYEP LGIVNLEAMA 
       310        320        330        340        350
CATAVVASDV GGIPEVVADG ITGSLVHYDA DDATGYQARL AEAVNALVAD 
       360        370        380 
PATAERYGHA GRQRCIQEFS WAYIAEQTLD IYRKVCA
Length:387
Mass (Da):41,533
Last modified:April 16, 2014 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBC4C4B66980BAAFF
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP43968.1

Protein sequence database of the Protein Information Resource

More...PIRi		B70610

NCBI Reference Sequences

More...RefSeqi		NP_215728.1, NC_000962.3
WP_003898773.1, NZ_NVQJ01000039.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		CCP43968; CCP43968; Rv1212c

Database of genes from NCBI RefSeq genomes

More...GeneIDi		887805

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mtu:Rv1212c
mtv:RVBD_1212c

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP43968.1
PIRiB70610
RefSeqiNP_215728.1, NC_000962.3
WP_003898773.1, NZ_NVQJ01000039.1

3D structure databases

SMRiP9WMZ1
ModBaseiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1212c

Proteomic databases

PaxDbiP9WMZ1

Genome annotation databases

EnsemblBacteriaiCCP43968; CCP43968; Rv1212c
GeneIDi887805
KEGGimtu:Rv1212c
mtv:RVBD_1212c

Organism-specific databases

TubercuListiRv1212c

Phylogenomic databases

eggNOGiENOG4107QPH Bacteria
COG0438 LUCA
KOiK16148
OMAiWYTGLAG
PhylomeDBiP9WMZ1

Enzyme and pathway databases

UniPathwayiUPA00164
UPA00934
BioCyciMetaCyc:G185E-5382-MONOMER
MTBH37RV:G185E-5382-MONOMER

Family and domain databases

InterProiView protein in InterPro
IPR001296 Glyco_trans_1
IPR028098 Glyco_trans_4-like_N
IPR011875 M1P_synthase
PfamiView protein in Pfam
PF13439 Glyco_transf_4, 1 hit
PF00534 Glycos_transf_1, 1 hit
TIGRFAMsiTIGR02149 glgA_Coryne, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGLGM_MYCTU
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P9WMZ1
Secondary accession number(s): L0T8Z4, O05313, Q7D8L6
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 16, 2019
This is version 33 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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