UniProtKB - P9WMP9 (UVRD2_MYCTU)
Protein
ATP-dependent DNA helicase UvrD2
Gene
uvrD2
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
DNA-dependent ATPase, stimulated equally by ss- and dsDNA. Has both ATPase and helicase activities, and translocates along ssDNA displacing bound streptavidin. Its essentiality for growth does not depend on its helicase activity.2 Publications
Catalytic activityi
- EC:3.6.4.12
Cofactori
Mg2+1 Publication
Kineticsi
kcat is 0.035 sec(-1) with ATP.
- KM=68.0 µM for ATP1 Publication
- KM=0.049 µM for ssDNA1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 299 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 34 – 39 | ATPPROSITE-ProRule annotation | 6 |
GO - Molecular functioni
- 3'-5' DNA helicase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- DNA-dependent ATPase activity Source: MTBBASE
- DNA helicase activity Source: MTBBASE
- magnesium ion binding Source: MTBBASE
GO - Biological processi
- DNA repair Source: GO_Central
- recombinational repair Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:uvrD2 Ordered Locus Names:Rv3198c ORF Names:MTV014.42c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3198c |
Subcellular locationi
GO - Cellular componenti
- cytosol Source: GO_Central
- DNA helicase complex Source: GO_Central
Pathology & Biotechi
Disruption phenotypei
Essential for growth; the 100 C-terminal residues are not required for growth.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 266 | Q → R: Loss of growth, no ATPase activity. No DNA unwinding. Does not translocate along DNA. 1 Publication | 1 | |
Mutagenesisi | 508 | E → A: Not essential for growth, no helicase activity. Wild-type ATPase activity, translocates along DNA. No DNA unwinding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102079 | 1 – 700 | ATP-dependent DNA helicase UvrD2Add BLAST | 700 |
Proteomic databases
PaxDbi | P9WMP9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 301 | UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 292 | |
Domaini | 302 – 553 | UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 252 | |
Domaini | 626 – 700 | HRDCPROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 601 – 700 | Not required for growthAdd BLAST | 100 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0210, Bacteria |
OMAi | LLLYWAQ |
PhylomeDBi | P9WMP9 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit |
InterProi | View protein in InterPro IPR013986, DExx_box_DNA_helicase_dom_sf IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR010997, HRDC-like_sf IPR002121, HRDC_dom IPR027417, P-loop_NTPase IPR014016, UvrD-like_ATP-bd IPR034739, UvrD/AddA_N |
PANTHERi | PTHR11070, PTHR11070, 1 hit |
Pfami | View protein in Pfam PF00570, HRDC, 1 hit PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 2 hits |
SMARTi | View protein in SMART SM00341, HRDC, 1 hit |
SUPFAMi | SSF47819, SSF47819, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50967, HRDC, 1 hit PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WMP9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIASDPLIA GLDDQQREAV LAPRGPVCVL AGAGTGKTRT ITHRIASLVA
60 70 80 90 100
SGHVAAGQVL AVTFTQRAAG EMRSRLRALD AAARTGSGVG AVQALTFHAA
110 120 130 140 150
AYRQLRYFWS RVIADTGWQL LDSKFAVVAR AASRTRLHAS TDDVRDLAGE
160 170 180 190 200
IEWAKASLIG PEEYVTAVAA ARRDPPLDAA QIAAVYSEYE ALKARGDGVT
210 220 230 240 250
LLDFDDLLLH TAAAIENDAA VAEEFQDRYR CFVVDEYQDV TPLQQRVLSA
260 270 280 290 300
WLGDRDDLTV VGDANQTIYS FTGASPRFLL DFSRRFPDAA VVRLERDYRS
310 320 330 340 350
TPQVVSLANR VIAAARGRVA GSKLRLSGQR EPGPVPSFHE HSDEPAEAAT
360 370 380 390 400
VAASIARLIA SGTPPSEVAI LYRVNAQSEV YEEALTQAGI AYQVRGGEGF
410 420 430 440 450
FNRQEIKQAL LALQRVSERD TDAALSDVVR AVLAPLGLTA QPPVGTRARE
460 470 480 490 500
RWEALTALAE LVDDELAQRP ALQLPGLLAE LRRRAEARHP PVVQGVTLAS
510 520 530 540 550
LHAAKGLEWD AVFLVGLADG TLPISHALAH GPNSEPVEEE RRLLYVGITR
560 570 580 590 600
ARVHLALSWA LSRSPGGRQS RKPSRFLNGI APQTRADPVP GTSRRNRGAA
610 620 630 640 650
ARCRICNNEL NTSAAVMLRR CETCAADVDE ELLLQLKSWR LSTAKEQNVP
660 670 680 690 700
AYVVFTDNTL IAIAELLPTD DAALIAIPGI GARKLEQYGS DVLQLVRGRT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46012.1 |
PIRi | D70951 |
RefSeqi | NP_217714.1, NC_000962.3 WP_003416822.1, NZ_NVQJ01000003.1 |
Genome annotation databases
EnsemblBacteriai | CCP46012; CCP46012; Rv3198c |
GeneIDi | 45427188 888902 |
KEGGi | mtu:Rv3198c |
PATRICi | fig|83332.111.peg.3569 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46012.1 |
PIRi | D70951 |
RefSeqi | NP_217714.1, NC_000962.3 WP_003416822.1, NZ_NVQJ01000003.1 |
3D structure databases
SMRi | P9WMP9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3198c |
Proteomic databases
PaxDbi | P9WMP9 |
Genome annotation databases
EnsemblBacteriai | CCP46012; CCP46012; Rv3198c |
GeneIDi | 45427188 888902 |
KEGGi | mtu:Rv3198c |
PATRICi | fig|83332.111.peg.3569 |
Organism-specific databases
TubercuListi | Rv3198c |
Phylogenomic databases
eggNOGi | COG0210, Bacteria |
OMAi | LLLYWAQ |
PhylomeDBi | P9WMP9 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit |
InterProi | View protein in InterPro IPR013986, DExx_box_DNA_helicase_dom_sf IPR014017, DNA_helicase_UvrD-like_C IPR000212, DNA_helicase_UvrD/REP IPR010997, HRDC-like_sf IPR002121, HRDC_dom IPR027417, P-loop_NTPase IPR014016, UvrD-like_ATP-bd IPR034739, UvrD/AddA_N |
PANTHERi | PTHR11070, PTHR11070, 1 hit |
Pfami | View protein in Pfam PF00570, HRDC, 1 hit PF00580, UvrD-helicase, 1 hit PF13361, UvrD_C, 2 hits |
SMARTi | View protein in SMART SM00341, HRDC, 1 hit |
SUPFAMi | SSF47819, SSF47819, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50967, HRDC, 1 hit PS51198, UVRD_HELICASE_ATP_BIND, 1 hit PS51217, UVRD_HELICASE_CTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UVRD2_MYCTU | |
Accessioni | P9WMP9Primary (citable) accession number: P9WMP9 Secondary accession number(s): L0TBT8, O53344, P64320 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 44 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families