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Entry version 38 (16 Oct 2019)
Sequence version 1 (16 Apr 2014)
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Protein

ATP-dependent DNA helicase UvrD2

Gene

uvrD2

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA-dependent ATPase, stimulated equally by ss-and dsDNA. Has both ATPase and helicase activities, and translocates along ssDNA displacing bound streptavidin. Its essentiality for growth does not depend on its helicase activity.2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.035 sec(-1) with ATP.
  1. KM=68.0 µM for ATP1 Publication
  2. KM=0.049 µM for ssDNA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei299ATPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi34 – 39ATPPROSITE-ProRule annotation6

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionDNA-binding, Helicase, Hydrolase
    Biological processDNA damage, DNA repair
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MTBH37RV:G185E-7467-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    ATP-dependent DNA helicase UvrD2 (EC:3.6.4.12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:uvrD2
    Ordered Locus Names:Rv3198c
    ORF Names:MTV014.42c
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83332 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000001584 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    Organism-specific databases

    Mycobacterium tuberculosis strain H37Rv genome database

    More...
    TubercuListi
    Rv3198c

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Essential for growth; the 100 C-terminal residues are not required for growth.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi266Q → R: Loss of growth, no ATPase activity. No DNA unwinding. Does not translocate along DNA. 1 Publication1
    Mutagenesisi508E → A: Not essential for growth, no helicase activity. Wild-type ATPase activity, translocates along DNA. No DNA unwinding. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001020791 – 700ATP-dependent DNA helicase UvrD2Add BLAST700

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P9WMP9

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    83332.Rv3198c

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P9WMP9

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini10 – 301UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST292
    Domaini302 – 553UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST252
    Domaini626 – 700HRDCPROSITE-ProRule annotationAdd BLAST75

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni601 – 700Not required for growthAdd BLAST100

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the helicase family. UvrD subfamily.Curated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105C4R Bacteria
    COG0210 LUCA

    KEGG Orthology (KO)

    More...
    KOi
    K03657

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    LLLYWAQ

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P9WMP9

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    1.10.10.160, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR013986 DExx_box_DNA_helicase_dom_sf
    IPR014017 DNA_helicase_UvrD-like_C
    IPR000212 DNA_helicase_UvrD/REP
    IPR010997 HRDC-like_sf
    IPR002121 HRDC_dom
    IPR027417 P-loop_NTPase
    IPR014016 UvrD-like_ATP-bd
    IPR034739 UvrD/AddA_N

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR11070 PTHR11070, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00570 HRDC, 1 hit
    PF00580 UvrD-helicase, 1 hit
    PF13361 UvrD_C, 2 hits

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00341 HRDC, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF47819 SSF47819, 1 hit
    SSF52540 SSF52540, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS50967 HRDC, 1 hit
    PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
    PS51217 UVRD_HELICASE_CTER, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P9WMP9-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSIASDPLIA GLDDQQREAV LAPRGPVCVL AGAGTGKTRT ITHRIASLVA
    60 70 80 90 100
    SGHVAAGQVL AVTFTQRAAG EMRSRLRALD AAARTGSGVG AVQALTFHAA
    110 120 130 140 150
    AYRQLRYFWS RVIADTGWQL LDSKFAVVAR AASRTRLHAS TDDVRDLAGE
    160 170 180 190 200
    IEWAKASLIG PEEYVTAVAA ARRDPPLDAA QIAAVYSEYE ALKARGDGVT
    210 220 230 240 250
    LLDFDDLLLH TAAAIENDAA VAEEFQDRYR CFVVDEYQDV TPLQQRVLSA
    260 270 280 290 300
    WLGDRDDLTV VGDANQTIYS FTGASPRFLL DFSRRFPDAA VVRLERDYRS
    310 320 330 340 350
    TPQVVSLANR VIAAARGRVA GSKLRLSGQR EPGPVPSFHE HSDEPAEAAT
    360 370 380 390 400
    VAASIARLIA SGTPPSEVAI LYRVNAQSEV YEEALTQAGI AYQVRGGEGF
    410 420 430 440 450
    FNRQEIKQAL LALQRVSERD TDAALSDVVR AVLAPLGLTA QPPVGTRARE
    460 470 480 490 500
    RWEALTALAE LVDDELAQRP ALQLPGLLAE LRRRAEARHP PVVQGVTLAS
    510 520 530 540 550
    LHAAKGLEWD AVFLVGLADG TLPISHALAH GPNSEPVEEE RRLLYVGITR
    560 570 580 590 600
    ARVHLALSWA LSRSPGGRQS RKPSRFLNGI APQTRADPVP GTSRRNRGAA
    610 620 630 640 650
    ARCRICNNEL NTSAAVMLRR CETCAADVDE ELLLQLKSWR LSTAKEQNVP
    660 670 680 690 700
    AYVVFTDNTL IAIAELLPTD DAALIAIPGI GARKLEQYGS DVLQLVRGRT
    Length:700
    Mass (Da):75,604
    Last modified:April 16, 2014 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i60A1B7520855C81C
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AL123456 Genomic DNA Translation: CCP46012.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    D70951

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_217714.1, NC_000962.3
    WP_003416822.1, NZ_NVQJ01000003.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    CCP46012; CCP46012; Rv3198c

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    888902

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    mtu:Rv3198c
    mtv:RVBD_3198c

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|83332.111.peg.3569

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL123456 Genomic DNA Translation: CCP46012.1
    PIRiD70951
    RefSeqiNP_217714.1, NC_000962.3
    WP_003416822.1, NZ_NVQJ01000003.1

    3D structure databases

    SMRiP9WMP9
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi83332.Rv3198c

    Proteomic databases

    PaxDbiP9WMP9

    Genome annotation databases

    EnsemblBacteriaiCCP46012; CCP46012; Rv3198c
    GeneIDi888902
    KEGGimtu:Rv3198c
    mtv:RVBD_3198c
    PATRICifig|83332.111.peg.3569

    Organism-specific databases

    TubercuListiRv3198c

    Phylogenomic databases

    eggNOGiENOG4105C4R Bacteria
    COG0210 LUCA
    KOiK03657
    OMAiLLLYWAQ
    PhylomeDBiP9WMP9

    Enzyme and pathway databases

    BioCyciMTBH37RV:G185E-7467-MONOMER

    Family and domain databases

    Gene3Di1.10.10.160, 1 hit
    InterProiView protein in InterPro
    IPR013986 DExx_box_DNA_helicase_dom_sf
    IPR014017 DNA_helicase_UvrD-like_C
    IPR000212 DNA_helicase_UvrD/REP
    IPR010997 HRDC-like_sf
    IPR002121 HRDC_dom
    IPR027417 P-loop_NTPase
    IPR014016 UvrD-like_ATP-bd
    IPR034739 UvrD/AddA_N
    PANTHERiPTHR11070 PTHR11070, 1 hit
    PfamiView protein in Pfam
    PF00570 HRDC, 1 hit
    PF00580 UvrD-helicase, 1 hit
    PF13361 UvrD_C, 2 hits
    SMARTiView protein in SMART
    SM00341 HRDC, 1 hit
    SUPFAMiSSF47819 SSF47819, 1 hit
    SSF52540 SSF52540, 1 hit
    PROSITEiView protein in PROSITE
    PS50967 HRDC, 1 hit
    PS51198 UVRD_HELICASE_ATP_BIND, 1 hit
    PS51217 UVRD_HELICASE_CTER, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiUVRD2_MYCTU
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P9WMP9
    Secondary accession number(s): L0TBT8, O53344, P64320
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 16, 2014
    Last modified: October 16, 2019
    This is version 38 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
      Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
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