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Protein

DNA-binding transcriptional activator DevR/DosR

Gene

devR

Organism
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Member of the two-component regulatory system DevR/DevS (DosR/DosS) involved in onset of the dormancy response. Regulates an approximately 48-member regulon (PubMed:12953092). When phosphorylated binds and activates the promoter of DevR regulon genes in response to hypoxia (By similarity). Accepts a phosphate group from DevS (DosS) and from DosT (By similarity). Does not regulate transcription of dosT (By similarity).By similarity1 Publication

Miscellaneous

The dev nomenclature derives from the increased expression (differentially expressed in virulent strain, dev) of these genes in virulent H37Rv versus avirulent H37Ra. The dos nomenclature derives from experiments in M.bovis showing the same genes are essential for dormancy survival.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi167 – 186H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation, Two-component regulatory system

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding transcriptional activator DevR/DosRCurated
Gene namesi
Name:devR
Synonyms:dosR
Ordered Locus Names:MT3219
OrganismiMycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Taxonomic identifieri83331 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001020 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity
  • host phagosome By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Host cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004273111 – 217DNA-binding transcriptional activator DevR/DosRAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei544-aspartylphosphatePROSITE-ProRule annotation1
Modified residuei198Phosphothreonine; by PknHBy similarity1
Modified residuei205Phosphothreonine; by PknHBy similarity1

Post-translational modificationi

Phosphorylated on Asp-54 by both DevS (DosS) and DosT. Phosphorylated on Thr-198 and Thr-205 by PknH. Phosphorylation by PknH enhances DevR dimerization. Aspartate phosphorylation and threonine phosphorylation cooperatively enhance DevR binding to DNA (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

A member of the dormancy regulon. Moderately expressed under aerobic conditions, it is strongly induced in response to reduced oxygen tension (hypoxia) and low levels of nitric oxide (NO).1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP9WMF8
SMRiP9WMF8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 119Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini143 – 208HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Phylogenomic databases

KOiK07695
OrthoDBiPOG091H05B9

Family and domain databases

CDDicd06170 LuxR_C_like, 1 hit
cd00156 REC, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR001789 Sig_transdc_resp-reg_receiver
IPR000792 Tscrpt_reg_LuxR_C
PfamiView protein in Pfam
PF00196 GerE, 1 hit
PF00072 Response_reg, 1 hit
PRINTSiPR00038 HTHLUXR
SMARTiView protein in SMART
SM00421 HTH_LUXR, 1 hit
SM00448 REC, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
SSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS00622 HTH_LUXR_1, 1 hit
PS50043 HTH_LUXR_2, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P9WMF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVFLVDDH EVVRRGLVDL LGADPELDVV GEAGSVAEAM ARVPAARPDV
60 70 80 90 100
AVLDVRLPDG NGIELCRDLL SRMPDLRCLI LTSYTSDEAM LDAILAGASG
110 120 130 140 150
YVVKDIKGME LARAVKDVGA GRSLLDNRAA AALMAKLRGA AEKQDPLSGL
160 170 180 190 200
TDQERTLLGL LSEGLTNKQI ADRMFLAEKT VKNYVSRLLA KLGMERRTQA
210
AVFATELKRS RPPGDGP
Length:217
Mass (Da):23,294
Last modified:April 16, 2014 - v1
Checksum:i1BA535C26CC4EB51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000516 Genomic DNA Translation: AAK47557.1
PIRiF70645
RefSeqiWP_003416369.1, NZ_KK341227.1

Genome annotation databases

EnsemblBacteriaiAAK47557; AAK47557; MT3219
KEGGimtc:MT3219
PATRICifig|83331.31.peg.3469

Entry informationi

Entry nameiDEVR_MYCTO
AccessioniPrimary (citable) accession number: P9WMF8
Secondary accession number(s): L0TBX9
, P95193, Q79CX8, Q7D625
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: February 28, 2018
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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