UniProtKB - P9WKJ3 (ILVH_MYCTU)
Protein
Putative acetolactate synthase small subunit
Gene
ilvH
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.By similarity
Catalytic activityi
- EC:2.2.1.6
: L-isoleucine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase large subunit IlvG (ilvG), Acetolactate synthase large subunit IlvB1 (ilvB1), Putative acetolactate synthase small subunit (ilvH), Putative acetolactate synthase large subunit IlvB2 (ilvB2), Putative acetolactate synthase large subunit IlvX (ilvX)
- Ketol-acid reductoisomerase (NADP(+)) (ilvC)
- Dihydroxy-acid dehydratase (ilvD)
- Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.
Pathwayi: L-valine biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes L-valine from pyruvate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- Acetolactate synthase large subunit IlvG (ilvG), Acetolactate synthase large subunit IlvB1 (ilvB1), Putative acetolactate synthase small subunit (ilvH), Putative acetolactate synthase large subunit IlvB2 (ilvB2), Putative acetolactate synthase large subunit IlvX (ilvX)
- Ketol-acid reductoisomerase (NADP(+)) (ilvC)
- Dihydroxy-acid dehydratase (ilvD)
- Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- acetolactate synthase activity Source: GO_Central
- acetolactate synthase regulator activity Source: InterPro
GO - Biological processi
- isoleucine biosynthetic process Source: GO_Central
- valine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7260-MONOMER |
UniPathwayi | UPA00047;UER00055 UPA00049;UER00059 |
Names & Taxonomyi
Protein namesi | Recommended name: Putative acetolactate synthase small subunit (EC:2.2.1.6)Alternative name(s): Acetohydroxy-acid synthase small subunit Short name: AHAS Short name: ALS |
Gene namesi | Name:ilvH Synonyms:ilvN Ordered Locus Names:Rv3002c ORF Names:MTV012.16c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3002c |
Subcellular locationi
GO - Cellular componenti
- acetolactate synthase complex Source: GO_Central
- cell wall Source: MTBBASE
- cytosol Source: GO_Central
- plasma membrane Source: MTBBASE
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151416 | 1 – 168 | Putative acetolactate synthase small subunitAdd BLAST | 168 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 44 | Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)1 Publication |
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | P9WKJ3 |
Expressioni
Inductioni
The expression gradually decreases as growth progresses.1 Publication
Interactioni
Subunit structurei
Dimer of large and small chains.
By similarityProtein-protein interaction databases
STRINGi | 83332.Rv3002c |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 82 | ACTPROSITE-ProRule annotationAdd BLAST | 76 |
Sequence similaritiesi
Belongs to the acetolactate synthase small subunit family.Curated
Phylogenomic databases
eggNOGi | ENOG4108ZP8 Bacteria COG0440 LUCA |
KOi | K01653 |
OMAi | EHPDISR |
PhylomeDBi | P9WKJ3 |
Family and domain databases
CDDi | cd04878 ACT_AHAS, 1 hit |
Gene3Di | 3.30.70.1150, 1 hit |
InterProi | View protein in InterPro IPR004789 Acetalactate_synth_ssu IPR027271 Acetolactate_synth/TF_NikR_C IPR019455 Acetolactate_synth_ssu_C IPR002912 ACT_dom IPR039557 AHAS_ACT |
PANTHERi | PTHR30239 PTHR30239, 1 hit |
Pfami | View protein in Pfam PF10369 ALS_ss_C, 1 hit |
TIGRFAMsi | TIGR00119 acolac_sm, 1 hit |
PROSITEi | View protein in PROSITE PS51671 ACT, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WKJ3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSPKTHTLSV LVEDKPGVLA RVAALFSRRG FNIESLAVGA TECKDRSRMT
60 70 80 90 100
IVVSAEDTPL EQITKQLNKL INVIKIVEQD DEHSVSRELA LIKVQADAGS
110 120 130 140 150
RSQVIEAVNL FRANVIDVSP ESLTVEATGN RGKLEALLRV LEPFGIREIA
160
QSGMVSLSRG PRGIGTAK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45808.1 |
PIRi | E70855 |
RefSeqi | NP_217518.1, NC_000962.3 WP_003415167.1, NZ_NVQJ01000041.1 |
Genome annotation databases
EnsemblBacteriai | CCP45808; CCP45808; Rv3002c |
GeneIDi | 887226 |
KEGGi | mtu:Rv3002c mtv:RVBD_3002c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45808.1 |
PIRi | E70855 |
RefSeqi | NP_217518.1, NC_000962.3 WP_003415167.1, NZ_NVQJ01000041.1 |
3D structure databases
SMRi | P9WKJ3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3002c |
Proteomic databases
PaxDbi | P9WKJ3 |
Genome annotation databases
EnsemblBacteriai | CCP45808; CCP45808; Rv3002c |
GeneIDi | 887226 |
KEGGi | mtu:Rv3002c mtv:RVBD_3002c |
Organism-specific databases
TubercuListi | Rv3002c |
Phylogenomic databases
eggNOGi | ENOG4108ZP8 Bacteria COG0440 LUCA |
KOi | K01653 |
OMAi | EHPDISR |
PhylomeDBi | P9WKJ3 |
Enzyme and pathway databases
UniPathwayi | UPA00047;UER00055 UPA00049;UER00059 |
BioCyci | MTBH37RV:G185E-7260-MONOMER |
Family and domain databases
CDDi | cd04878 ACT_AHAS, 1 hit |
Gene3Di | 3.30.70.1150, 1 hit |
InterProi | View protein in InterPro IPR004789 Acetalactate_synth_ssu IPR027271 Acetolactate_synth/TF_NikR_C IPR019455 Acetolactate_synth_ssu_C IPR002912 ACT_dom IPR039557 AHAS_ACT |
PANTHERi | PTHR30239 PTHR30239, 1 hit |
Pfami | View protein in Pfam PF10369 ALS_ss_C, 1 hit |
TIGRFAMsi | TIGR00119 acolac_sm, 1 hit |
PROSITEi | View protein in PROSITE PS51671 ACT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILVH_MYCTU | |
Accessioni | P9WKJ3Primary (citable) accession number: P9WKJ3 Secondary accession number(s): L0TE14, O53249, P65161 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 30 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways