UniProtKB - P9WKE5 (KPYK_MYCTU)
Protein
Pyruvate kinase
Gene
pyk
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalytic activityi
- EC:2.7.1.401 Publication
Cofactori
Protein has several cofactor binding sites:: glycolysis Pathwayi
This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase (gap)
- Phosphoglycerate kinase (pgk)
- 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
- Enolase (eno)
- Pyruvate kinase (pyk)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | SubstrateBy similarity | 1 | |
Metal bindingi | 35 | PotassiumBy similarity | 1 | |
Metal bindingi | 37 | PotassiumBy similarity | 1 | |
Metal bindingi | 67 | PotassiumBy similarity | 1 | |
Binding sitei | 74 | ATPBy similarity | 1 | |
Binding sitei | 155 | ATPBy similarity | 1 | |
Sitei | 218 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 220 | MagnesiumBy similarity | 1 | |
Binding sitei | 243 | Substrate; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 244 | MagnesiumBy similarity | 1 | |
Binding sitei | 244 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 276 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 35 – 38 | ATPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase activity Source: UniProtKB-KW
- magnesium ion binding Source: InterPro
- potassium ion binding Source: InterPro
- pyruvate kinase activity Source: GO_Central
GO - Biological processi
- glycolytic process Source: GO_Central
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Glycolysis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-5805-MONOMER |
UniPathwayi | UPA00109;UER00188 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pyk Synonyms:pykA Ordered Locus Names:Rv1617 ORF Names:MTCY01B2.09 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1617 |
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: GO_Central
- cytosol Source: MTBBASE
- plasma membrane Source: MTBBASE
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 37 | S → A: Partial loss of phosphorylation. Decrease in activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000112082 | 1 – 472 | Pyruvate kinaseAdd BLAST | 472 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 37 | Phosphoserine; by PknJ; in vitro1 Publication | 1 |
Post-translational modificationi
Phosphorylated by PknJ. Dephosphorylated by PstP.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P9WKE5 |
PTM databases
iPTMneti | P9WKE5 |
Interactioni
Subunit structurei
Homotetramer.
By similarityProtein-protein interaction databases
STRINGi | 83332.Rv1617 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WKE5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the pyruvate kinase family.Curated
Phylogenomic databases
eggNOGi | ENOG4105CA9 Bacteria COG0469 LUCA |
KOi | K00873 |
OMAi | QVPIVQK |
PhylomeDBi | P9WKE5 |
Family and domain databases
Gene3Di | 2.40.33.10, 1 hit 3.20.20.60, 1 hit 3.40.1380.20, 1 hit |
InterProi | View protein in InterPro IPR001697 Pyr_Knase IPR015813 Pyrv/PenolPyrv_Kinase-like_dom IPR040442 Pyrv_Kinase-like_dom_sf IPR011037 Pyrv_Knase-like_insert_dom_sf IPR018209 Pyrv_Knase_AS IPR015793 Pyrv_Knase_brl IPR015795 Pyrv_Knase_C IPR036918 Pyrv_Knase_C_sf IPR015806 Pyrv_Knase_insert_dom_sf |
PANTHERi | PTHR11817 PTHR11817, 1 hit |
Pfami | View protein in Pfam PF00224 PK, 1 hit PF02887 PK_C, 1 hit |
PRINTSi | PR01050 PYRUVTKNASE |
SUPFAMi | SSF50800 SSF50800, 1 hit SSF51621 SSF51621, 1 hit SSF52935 SSF52935, 1 hit |
TIGRFAMsi | TIGR01064 pyruv_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00110 PYRUVATE_KINASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WKE5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRRGKIVCT LGPATQRDDL VRALVEAGMD VARMNFSHGD YDDHKVAYER
60 70 80 90 100
VRVASDATGR AVGVLADLQG PKIRLGRFAS GATHWAEGET VRITVGACEG
110 120 130 140 150
SHDRVSTTYK RLAQDAVAGD RVLVDDGKVA LVVDAVEGDD VVCTVVEGGP
160 170 180 190 200
VSDNKGISLP GMNVTAPALS EKDIEDLTFA LNLGVDMVAL SFVRSPADVE
210 220 230 240 250
LVHEVMDRIG RRVPVIAKLE KPEAIDNLEA IVLAFDAVMV ARGDLGVELP
260 270 280 290 300
LEEVPLVQKR AIQMARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV
310 320 330 340 350
LDGADALMLS GETSVGKYPL AAVRTMSRII CAVEENSTAA PPLTHIPRTK
360 370 380 390 400
RGVISYAARD IGERLDAKAL VAFTQSGDTV RRLARLHTPL PLLAFTAWPE
410 420 430 440 450
VRSQLAMTWG TETFIVPKMQ STDGMIRQVD KSLLELARYK RGDLVVIVAG
460 470
APPGTVGSTN LIHVHRIGED DV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44381.1 |
PIRi | G70557 |
RefSeqi | NP_216133.1, NC_000962.3 WP_003898945.1, NZ_NVQJ01000016.1 |
Genome annotation databases
EnsemblBacteriai | CCP44381; CCP44381; Rv1617 |
GeneIDi | 885501 |
KEGGi | mtu:Rv1617 mtv:RVBD_1617 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44381.1 |
PIRi | G70557 |
RefSeqi | NP_216133.1, NC_000962.3 WP_003898945.1, NZ_NVQJ01000016.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5WRP | X-ray | 2.85 | A/B/C/D | 1-472 | [»] | |
5WS8 | X-ray | 2.62 | A/B/C/D | 1-472 | [»] | |
5WS9 | X-ray | 1.90 | A/B/C/D | 1-472 | [»] | |
5WSA | X-ray | 2.85 | A/B/C/D | 1-472 | [»] | |
5WSB | X-ray | 2.25 | A/B/C/D | 1-472 | [»] | |
5WSC | X-ray | 2.40 | A/B/C/D | 1-472 | [»] | |
6ITO | X-ray | 2.55 | A/B/C/D | 1-472 | [»] | |
SMRi | P9WKE5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1617 |
PTM databases
iPTMneti | P9WKE5 |
Proteomic databases
PaxDbi | P9WKE5 |
Genome annotation databases
EnsemblBacteriai | CCP44381; CCP44381; Rv1617 |
GeneIDi | 885501 |
KEGGi | mtu:Rv1617 mtv:RVBD_1617 |
Organism-specific databases
TubercuListi | Rv1617 |
Phylogenomic databases
eggNOGi | ENOG4105CA9 Bacteria COG0469 LUCA |
KOi | K00873 |
OMAi | QVPIVQK |
PhylomeDBi | P9WKE5 |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00188 |
BioCyci | MTBH37RV:G185E-5805-MONOMER |
Family and domain databases
Gene3Di | 2.40.33.10, 1 hit 3.20.20.60, 1 hit 3.40.1380.20, 1 hit |
InterProi | View protein in InterPro IPR001697 Pyr_Knase IPR015813 Pyrv/PenolPyrv_Kinase-like_dom IPR040442 Pyrv_Kinase-like_dom_sf IPR011037 Pyrv_Knase-like_insert_dom_sf IPR018209 Pyrv_Knase_AS IPR015793 Pyrv_Knase_brl IPR015795 Pyrv_Knase_C IPR036918 Pyrv_Knase_C_sf IPR015806 Pyrv_Knase_insert_dom_sf |
PANTHERi | PTHR11817 PTHR11817, 1 hit |
Pfami | View protein in Pfam PF00224 PK, 1 hit PF02887 PK_C, 1 hit |
PRINTSi | PR01050 PYRUVTKNASE |
SUPFAMi | SSF50800 SSF50800, 1 hit SSF51621 SSF51621, 1 hit SSF52935 SSF52935, 1 hit |
TIGRFAMsi | TIGR01064 pyruv_kin, 1 hit |
PROSITEi | View protein in PROSITE PS00110 PYRUVATE_KINASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KPYK_MYCTU | |
Accessioni | P9WKE5Primary (citable) accession number: P9WKE5 Secondary accession number(s): L0T783, O06134 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 36 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references