UniProtKB - P9WKD9 (KU_MYCTU)
Protein
Non-homologous end joining protein Ku
Gene
mku
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
With LigD forms a non-homologous end joining (NHEJ) repair enzyme. Binds linear dsDNA with 5'- and 3'-overhangs but not closed circular dsDNA nor ssDNA. One dimer binds for every 30 bp. Recruits and stimulates the ligase activity of LigD but not of T4 ligase or a human ligase complex (LIG4/XRCC4). Attenuates the 3'- to 5'-exonuclease activity of LigD. Stimulates the template-directed addition of dNTPs by LigD on 5'-overhangs and nuclease activity on 3'-overhangs.4 Publications
GO - Molecular functioni
- double-stranded DNA binding Source: UniProtKB
- protein homodimerization activity Source: MTBBASE
GO - Biological processi
- DNA recombination Source: UniProtKB-KW
- double-strand break repair via nonhomologous end joining Source: UniProtKB
- positive regulation of ligase activity Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair |
Names & Taxonomyi
Protein namesi | Recommended name: Non-homologous end joining protein KuUniRule annotationAlternative name(s): Mt-Ku |
Gene namesi | Name:mku Ordered Locus Names:Rv0937c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0937c |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000425945 | 1 – 273 | Non-homologous end joining protein KuAdd BLAST | 273 |
Proteomic databases
PaxDbi | P9WKD9 |
Interactioni
Subunit structurei
Homodimer (Probable).
Interacts with LigD in the absence of DSBs, and with the isolated Pol domain of LigD in the presence of DNA.
Interacts with Sir2, possibly in a trimeric complex with LigD.
UniRule annotationCurated4 PublicationsGO - Molecular functioni
- protein homodimerization activity Source: MTBBASE
Protein-protein interaction databases
STRINGi | 83332.Rv0937c |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 193 | KuUniRule annotationAdd BLAST | 184 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 111 – 273 | Sufficient for interaction with LigDAdd BLAST | 163 |
Sequence similaritiesi
Belongs to the prokaryotic Ku family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1273, Bacteria |
OMAi | IRYRKVC |
PhylomeDBi | P9WKD9 |
Family and domain databases
CDDi | cd00789, KU_like, 1 hit |
Gene3Di | 2.40.290.10, 1 hit |
HAMAPi | MF_01875, Prokaryotic_Ku, 1 hit |
InterProi | View protein in InterPro IPR006164, Ku70/Ku80_beta-barrel_dom IPR009187, Prok_Ku IPR016194, SPOC-like_C_dom_sf |
PANTHERi | PTHR41251, PTHR41251, 1 hit |
Pfami | View protein in Pfam PF02735, Ku, 1 hit |
PIRSFi | PIRSF006493, Prok_Ku, 1 hit |
SMARTi | View protein in SMART SM00559, Ku78, 1 hit |
SUPFAMi | SSF100939, SSF100939, 1 hit |
TIGRFAMsi | TIGR02772, Ku_bact, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WKD9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRAIWTGSIA FGLVNVPVKV YSATADHDIR FHQVHAKDNG RIRYKRVCEA
60 70 80 90 100
CGEVVDYRDL ARAYESGDGQ MVAITDDDIA SLPEERSREI EVLEFVPAAD
110 120 130 140 150
VDPMMFDRSY FLEPDSKSSK SYVLLAKTLA ETDRMAIVHF TLRNKTRLAA
160 170 180 190 200
LRVKDFGKRE VMMVHTLLWP DEIRDPDFPV LDQKVEIKPA ELKMAGQVVD
210 220 230 240 250
SMADDFNPDR YHDTYQEQLQ ELIDTKLEGG QAFTAEDQPR LLDEPEDVSD
260 270
LLAKLEASVK ARSKANSNVP TPP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43685.1 |
PIRi | A70585 |
RefSeqi | NP_215452.1, NC_000962.3 WP_003404795.1, NZ_NVQJ01000001.1 |
Genome annotation databases
EnsemblBacteriai | CCP43685; CCP43685; Rv0937c |
GeneIDi | 885050 |
KEGGi | mtu:Rv0937c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43685.1 |
PIRi | A70585 |
RefSeqi | NP_215452.1, NC_000962.3 WP_003404795.1, NZ_NVQJ01000001.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 83332.Rv0937c |
Proteomic databases
PaxDbi | P9WKD9 |
Genome annotation databases
EnsemblBacteriai | CCP43685; CCP43685; Rv0937c |
GeneIDi | 885050 |
KEGGi | mtu:Rv0937c |
Organism-specific databases
TubercuListi | Rv0937c |
Phylogenomic databases
eggNOGi | COG1273, Bacteria |
OMAi | IRYRKVC |
PhylomeDBi | P9WKD9 |
Family and domain databases
CDDi | cd00789, KU_like, 1 hit |
Gene3Di | 2.40.290.10, 1 hit |
HAMAPi | MF_01875, Prokaryotic_Ku, 1 hit |
InterProi | View protein in InterPro IPR006164, Ku70/Ku80_beta-barrel_dom IPR009187, Prok_Ku IPR016194, SPOC-like_C_dom_sf |
PANTHERi | PTHR41251, PTHR41251, 1 hit |
Pfami | View protein in Pfam PF02735, Ku, 1 hit |
PIRSFi | PIRSF006493, Prok_Ku, 1 hit |
SMARTi | View protein in SMART SM00559, Ku78, 1 hit |
SUPFAMi | SSF100939, SSF100939, 1 hit |
TIGRFAMsi | TIGR02772, Ku_bact, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KU_MYCTU | |
Accessioni | P9WKD9Primary (citable) accession number: P9WKD9 Secondary accession number(s): L7N5V9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | February 10, 2021 | |
This is version 32 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families