UniProtKB - P9WKA1 (LEP_MYCTU)
Protein
Signal peptidase I
Gene
lepB
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Miscellaneous
Was identified as a high-confidence drug target.
Catalytic activityi
- Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.1 Publication EC:3.4.21.89
Activity regulationi
Inhibited by 1-(2,5-dichlorophenyl)-3-(dimethylamino)propan-1-one (MD3).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 96 | Curated | 1 | |
Active sitei | 174 | Curated | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: GO_Central
GO - Biological processi
- signal peptide processing Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7155-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Signal peptidase I (EC:3.4.21.89)Short name: SPase I Alternative name(s): Leader peptidase I |
Gene namesi | Name:lepB Ordered Locus Names:Rv2903c ORF Names:MTCY274.34c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2903c |
Subcellular locationi
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 66 | CytoplasmicSequence analysisAdd BLAST | 66 | |
Transmembranei | 67 – 87 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 88 – 294 | ExtracellularSequence analysisAdd BLAST | 207 |
GO - Cellular componenti
- extracellular region Source: MTBBASE
- integral component of plasma membrane Source: MTBBASE
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109511 | 1 – 294 | Signal peptidase IAdd BLAST | 294 |
Proteomic databases
PaxDbi | P9WKA1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S26 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG4107QYR Bacteria COG0681 LUCA |
KOi | K03100 |
OMAi | VECCDDQ |
PhylomeDBi | P9WKA1 |
Family and domain databases
InterProi | View protein in InterPro IPR036286 LexA/Signal_pep-like_sf IPR000223 Pept_S26A_signal_pept_1 IPR019758 Pept_S26A_signal_pept_1_CS IPR019756 Pept_S26A_signal_pept_1_Ser-AS IPR015927 Peptidase_S24_S26A/B/C IPR019533 Peptidase_S26 |
Pfami | View protein in Pfam PF00717 Peptidase_S24, 1 hit PF10502 Peptidase_S26, 1 hit |
PRINTSi | PR00727 LEADERPTASE |
SUPFAMi | SSF51306 SSF51306, 1 hit |
TIGRFAMsi | TIGR02227 sigpep_I_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00501 SPASE_I_1, 1 hit PS00761 SPASE_I_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WKA1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTETTDSPSE RQPGPAEPEL SSRDPDIAGQ VFDAAPFDAA PDADSEGDSK
60 70 80 90 100
AAKTDEPRPA KRSTLREFAV LAVIAVVLYY VMLTFVARPY LIPSESMEPT
110 120 130 140 150
LHGCSTCVGD RIMVDKLSYR FGSPQPGDVI VFRGPPSWNV GYKSIRSHNV
160 170 180 190 200
AVRWVQNALS FIGFVPPDEN DLVKRVIAVG GQTVQCRSDT GLTVNGRPLK
210 220 230 240 250
EPYLDPATMM ADPSIYPCLG SEFGPVTVPP GRVWVMGDNR THSADSRAHC
260 270 280 290
PLLCTDDPLP GTVPVANVIG KARLIVWPPS RWGVVRSVNP QQGR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45705.1 |
PIRi | B70927 |
RefSeqi | NP_217419.1, NC_000962.3 WP_003414715.1, NZ_NVQJ01000006.1 |
Genome annotation databases
EnsemblBacteriai | CCP45705; CCP45705; Rv2903c |
GeneIDi | 887157 |
KEGGi | mtu:Rv2903c mtv:RVBD_2903c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45705.1 |
PIRi | B70927 |
RefSeqi | NP_217419.1, NC_000962.3 WP_003414715.1, NZ_NVQJ01000006.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 83332.Rv2903c |
Proteomic databases
PaxDbi | P9WKA1 |
Genome annotation databases
EnsemblBacteriai | CCP45705; CCP45705; Rv2903c |
GeneIDi | 887157 |
KEGGi | mtu:Rv2903c mtv:RVBD_2903c |
Organism-specific databases
TubercuListi | Rv2903c |
Phylogenomic databases
eggNOGi | ENOG4107QYR Bacteria COG0681 LUCA |
KOi | K03100 |
OMAi | VECCDDQ |
PhylomeDBi | P9WKA1 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7155-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR036286 LexA/Signal_pep-like_sf IPR000223 Pept_S26A_signal_pept_1 IPR019758 Pept_S26A_signal_pept_1_CS IPR019756 Pept_S26A_signal_pept_1_Ser-AS IPR015927 Peptidase_S24_S26A/B/C IPR019533 Peptidase_S26 |
Pfami | View protein in Pfam PF00717 Peptidase_S24, 1 hit PF10502 Peptidase_S26, 1 hit |
PRINTSi | PR00727 LEADERPTASE |
SUPFAMi | SSF51306 SSF51306, 1 hit |
TIGRFAMsi | TIGR02227 sigpep_I_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00501 SPASE_I_1, 1 hit PS00761 SPASE_I_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEP_MYCTU | |
Accessioni | P9WKA1Primary (citable) accession number: P9WKA1 Secondary accession number(s): L0TAZ5, Q10789 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 31 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names