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Protein

Putative phthiocerol dimycocerosate transporter LppX

Gene

lppX

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Might be involved in translocating phthiocerol dimycocerosates (PDIM) from the cell membrane to the outer membrane; PDIM forms part of the cell wall.1 Publication

GO - Molecular functioni

  • lipid transporter activity Source: MTBBASE

GO - Biological processi

  • DIM/DIP cell wall layer assembly Source: MTBBASE
  • growth of symbiont in host organelle Source: MTBBASE
  • pathogenesis Source: MTBBASE

Keywordsi

Biological processLipid transport, Transport, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Putative phthiocerol dimycocerosate transporter LppX1 Publication
Alternative name(s):
Lipoprotein LppX
Gene namesi
Name:lppX
Ordered Locus Names:Rv2945c
ORF Names:MTCY24G1.04
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv2945c

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

No growth phenotype when grown in culture, no release of phthiocerol dimycocerosates (PDIM) to the culture filtrate. Attenuated virulence, about 80-fold less recovery of bacteria from lungs of 3 week infected BALB/c mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26PROSITE-ProRule annotation1 PublicationAdd BLAST26
ChainiPRO_000001812127 – 233Putative phthiocerol dimycocerosate transporter LppXAdd BLAST207

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi27N-palmitoyl cysteine1 Publication1 Publication1
Lipidationi27S-diacylglycerol cysteine1 Publication1 Publication1

Post-translational modificationi

Modified by Lgt on Cys-27 with an S-linked diacylglycerol with a mixture of C16 and C19 fatty acids (palmitic and tuberculostearic acid), signal peptide is removed by LspA, modified by Lnt with an amide-linked mixture of C16 and C19 fatty acids, expressed in M.bovis and M.smegmatis (PubMed:19661058, PubMed:24093492). Hexose glycosylated in N-terminus between residues 27 and 55; there may be 2 sugar moieties in this region, expressed in M.bovis (PubMed:24093492).1 Publication1 Publication

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP9WK65

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv2945c

Structurei

Secondary structure

1233
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP9WK65
SMRiP9WK65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Forms a U-shaped beta-half-barrel with a large hydrophobic cavity (2835 Angstroms3) which is large enough to hold a single phthiocerol dimycocerosate (PDIM) molecule.1 Publication

Sequence similaritiesi

Belongs to the LppX/LprAFG lipoprotein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

OMAiEHRPRIR
PhylomeDBiP9WK65

Family and domain databases

CDDicd16334 LppX-like, 1 hit
InterProiView protein in InterPro
IPR029046 LolA/LolB/LppX
IPR009830 LppX/LprAFG
PfamiView protein in Pfam
PF07161 LppX_LprAFG, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD017293 DUF1396, 1 hit
SUPFAMiSSF89392 SSF89392, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P9WK65-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNDGKRAVTS AVLVVLGACL ALWLSGCSSP KPDAEEQGVP VSPTASDPAL
60 70 80 90 100
LAEIRQSLDA TKGLTSVHVA VRTTGKVDSL LGITSADVDV RANPLAAKGV
110 120 130 140 150
CTYNDEQGVP FRVQGDNISV KLFDDWSNLG SISELSTSRV LDPAAGVTQL
160 170 180 190 200
LSGVTNLQAQ GTEVIDGIST TKITGTIPAS SVKMLDPGAK SARPATVWIA
210 220 230
QDGSHHLVRA SIDLGSGSIQ LTQSKWNEPV NVD
Length:233
Mass (Da):24,140
Last modified:April 16, 2014 - v1
Checksum:iFFACCDB2C7C2E87F
GO

Mass spectrometryi

Molecular mass is 26300 Da from positions 27 - 233. Determined by MALDI. Expressed in M.bovis, lipidated and glycosylated.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45749.1
PIRiF70668
RefSeqiNP_217461.1, NC_000962.3
WP_003414872.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiCCP45749; CCP45749; Rv2945c
GeneIDi887956
KEGGimtu:Rv2945c

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA Translation: CCP45749.1
PIRiF70668
RefSeqiNP_217461.1, NC_000962.3
WP_003414872.1, NZ_KK339370.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BYOX-ray2.15A27-233[»]
ProteinModelPortaliP9WK65
SMRiP9WK65
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv2945c

Proteomic databases

PaxDbiP9WK65

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP45749; CCP45749; Rv2945c
GeneIDi887956
KEGGimtu:Rv2945c

Organism-specific databases

TubercuListiRv2945c

Phylogenomic databases

OMAiEHRPRIR
PhylomeDBiP9WK65

Family and domain databases

CDDicd16334 LppX-like, 1 hit
InterProiView protein in InterPro
IPR029046 LolA/LolB/LppX
IPR009830 LppX/LprAFG
PfamiView protein in Pfam
PF07161 LppX_LprAFG, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD017293 DUF1396, 1 hit
SUPFAMiSSF89392 SSF89392, 1 hit
PROSITEiView protein in PROSITE
PS51257 PROKAR_LIPOPROTEIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLPPX_MYCTU
AccessioniPrimary (citable) accession number: P9WK65
Secondary accession number(s): L0TCQ0, P65306, P96286
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 16, 2014
Last modified: October 25, 2017
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
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